C.N.R. Short-term Mobility Program 2008 Gabriella Pocsfalvi
... SSO2749. This protein shows conserved domain of Linocin_M18 bacteriocin protein. Many Grampositive bacteria produce antimicrobial peptides, generally termed bacteriocins. These polypeptides usually has less than 50 amino acid residues long cationic, contain an amphiphilic or hydrophobic region, and ...
... SSO2749. This protein shows conserved domain of Linocin_M18 bacteriocin protein. Many Grampositive bacteria produce antimicrobial peptides, generally termed bacteriocins. These polypeptides usually has less than 50 amino acid residues long cationic, contain an amphiphilic or hydrophobic region, and ...
Unidirectional tandem gene arrays
... Major expression systems are classified into procaryotic and eucaryotic. ...
... Major expression systems are classified into procaryotic and eucaryotic. ...
biochemistry/docs/Protein structure 1
... Primary sequence- The amino acid sequence of a polypeptide, listed from N-terminus to C-terminus. Secondary structure- Recurring structural feature of proteins stabilized exclusively by hydrogen bonds between peptide bond elements. Supersecondary structure- Recurring structural feature of proteins c ...
... Primary sequence- The amino acid sequence of a polypeptide, listed from N-terminus to C-terminus. Secondary structure- Recurring structural feature of proteins stabilized exclusively by hydrogen bonds between peptide bond elements. Supersecondary structure- Recurring structural feature of proteins c ...
File
... – Contain carbon, hydrogen, and oxygen • Carbon and hydrogen outnumber oxygen. (ex: Tristearin is a triglyceride that forms the principle fat in beef. (C57H110O6) ...
... – Contain carbon, hydrogen, and oxygen • Carbon and hydrogen outnumber oxygen. (ex: Tristearin is a triglyceride that forms the principle fat in beef. (C57H110O6) ...
Unit 3 Biochemistry - The Naked Science Society
... The most common elements found in living organisms include: ...
... The most common elements found in living organisms include: ...
ppt
... Translation is first step to form functional protein: • polypeptide chain must fold into appropriate conformation • often undergoes processing steps. ...
... Translation is first step to form functional protein: • polypeptide chain must fold into appropriate conformation • often undergoes processing steps. ...
MATERIALS AND METHODS Materials All chemicals used in the
... CGH buffer at 25 ºC at a flow rate of 0.3 mL/min, with detection at 280 nm. The column was calibrated with standard molecular weight markers. Circular Dichroism (CD) measurements- CD measurements were made on JASCO J810 spectropolarimeter calibrated with ammonium (+)-10-camphorsulfonate with 6 µM p ...
... CGH buffer at 25 ºC at a flow rate of 0.3 mL/min, with detection at 280 nm. The column was calibrated with standard molecular weight markers. Circular Dichroism (CD) measurements- CD measurements were made on JASCO J810 spectropolarimeter calibrated with ammonium (+)-10-camphorsulfonate with 6 µM p ...
Regulation
... D. Tryptophan biosynthesis is also negatively regulated but in a different way than the lac operon. 1. Tryptophan biosynthesis is induced: 2. Tryptophan biosynthesis is repressed when: ...
... D. Tryptophan biosynthesis is also negatively regulated but in a different way than the lac operon. 1. Tryptophan biosynthesis is induced: 2. Tryptophan biosynthesis is repressed when: ...
Primary Structure Specifies Tertiary Structure
... After the tertiary structures of proteins were first shown to be highly specific, the question arose as to how the order of amino acids determined the three-dimensional structure. The second protein whose structure was determined was ribonuclease A, an enzyme from cows that was readily available fro ...
... After the tertiary structures of proteins were first shown to be highly specific, the question arose as to how the order of amino acids determined the three-dimensional structure. The second protein whose structure was determined was ribonuclease A, an enzyme from cows that was readily available fro ...
Substances required for living processes
... When many amino acids join together a long-chain polypeptide is produced. The linking of amino acids in this way takes place during protein synthesis. There are around 20 different amino acids. Organisms join amino acids in different linear sequences to form a variety of polypeptides, then build th ...
... When many amino acids join together a long-chain polypeptide is produced. The linking of amino acids in this way takes place during protein synthesis. There are around 20 different amino acids. Organisms join amino acids in different linear sequences to form a variety of polypeptides, then build th ...
Membrane Transport
... – collapsing ion gradients – facilitating entry by toxic agents – introducing a harmful catalytic activity ...
... – collapsing ion gradients – facilitating entry by toxic agents – introducing a harmful catalytic activity ...
Document
... • HlyA lacks an N-terminal secretion signal-peptide, but is nonetheless secreted efficiently secretion involves a sec-independent pathway Employed by various Gram-neg. species Each pathway specific for a single protein - although can be > 1 Type I pathway in cell to secrete different proteins. Each ...
... • HlyA lacks an N-terminal secretion signal-peptide, but is nonetheless secreted efficiently secretion involves a sec-independent pathway Employed by various Gram-neg. species Each pathway specific for a single protein - although can be > 1 Type I pathway in cell to secrete different proteins. Each ...
Protein folding and structure
... Difference between a prion disease and amyloid disease Thermodynamic and kinetic hypothesis of protein folding Why it is difficult to observe directly transition state ...
... Difference between a prion disease and amyloid disease Thermodynamic and kinetic hypothesis of protein folding Why it is difficult to observe directly transition state ...
Fundamentals of protein stability
... correctness of the model. These high quality transition curves enable us to improve our results of the heat capacity change involved in unfolding of native BPTI (ref. 2). Due to instrumental limitations of the DASM 1 calorimeters used previously it was not possible to measure beyond 110OC. Therefore ...
... correctness of the model. These high quality transition curves enable us to improve our results of the heat capacity change involved in unfolding of native BPTI (ref. 2). Due to instrumental limitations of the DASM 1 calorimeters used previously it was not possible to measure beyond 110OC. Therefore ...
A comprehensive investigation of ribosomal genes in complete
... Genes often missed during annotation process ...
... Genes often missed during annotation process ...
Biochemistry Review
... 4. What type of structures can carbon atoms form? Ring, Straight and Branched Chains, Single, Double, and Triple Bonds 5. What type of reaction joins monomers to form a polymer? Condensation What type of reaction breaks apart a polymer into monomers? Hydrolysis 6. Name the chemical compound that sto ...
... 4. What type of structures can carbon atoms form? Ring, Straight and Branched Chains, Single, Double, and Triple Bonds 5. What type of reaction joins monomers to form a polymer? Condensation What type of reaction breaks apart a polymer into monomers? Hydrolysis 6. Name the chemical compound that sto ...
ENGINEERING PROTEINS
... For the reaction aA + bB cC + dD The equilibrium constant is: Kc = Cc Dd / Aa Bb One in which the reaction rate is directly proportional to the substrate concentration. A DNA segment responsible for a particular protein (DNA codes for RNA which, in turn codes for proteins) The full set of ...
... For the reaction aA + bB cC + dD The equilibrium constant is: Kc = Cc Dd / Aa Bb One in which the reaction rate is directly proportional to the substrate concentration. A DNA segment responsible for a particular protein (DNA codes for RNA which, in turn codes for proteins) The full set of ...
DISULFIDE GROUPS Disulfide bonds in proteins are
... applied to a column of Sephadex G-25, equilibrated with 0.1 M acetic acid. The column was developed with the same solvent. Titrations with p-mercuribenzoate (Sections 10-2 and A-2) can be used to quantitate the extent of reaction. The same procedure has also been applied to trypsinogen, chymotrypsin ...
... applied to a column of Sephadex G-25, equilibrated with 0.1 M acetic acid. The column was developed with the same solvent. Titrations with p-mercuribenzoate (Sections 10-2 and A-2) can be used to quantitate the extent of reaction. The same procedure has also been applied to trypsinogen, chymotrypsin ...
Transcription/Translation foldable
... foldable Fold your paper so the two ends meet in the middle. Label Transcription on one side and Translation on the other. ...
... foldable Fold your paper so the two ends meet in the middle. Label Transcription on one side and Translation on the other. ...
Protein Structure Predictions 1
... Regular patterns of hydrogen bonding in proteins result in two patterns that emerge in nearly every protein structure known: the -helix and the -sheet The location of direction of these periodic, repeating structures is known as the secondary structure of the protein ...
... Regular patterns of hydrogen bonding in proteins result in two patterns that emerge in nearly every protein structure known: the -helix and the -sheet The location of direction of these periodic, repeating structures is known as the secondary structure of the protein ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.