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Nutrients - Food a fact of life
Nutrients - Food a fact of life

... When too much carbohydrate is consumed and not used for energy over an extended period of time, it is stored as fat. Building up too much fat will increase body weight. Increase dental caries It is important that teeth are brushed twice a day and foods high in sugar should be eaten with main meals, ...
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I can - Net Start Class

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Expression of Hepatitis C Virus Structural Proteins in
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... showed that purification of the HCV structural proteins yields both the processed and aggregated forms of each protein. The 18 kDa and 22 kDa protein bands correspond to the processed HCV core protein expressed in yeast, in which the C-terminal hydrophobic domain (a.a. 154-191) is truncated [18, 20] ...


... c) (4 pts) You prepare a scientific paper on these experiments, in which you claim that the energy of a SH hydrogen bond is 10 kJ/mol weaker than the energy of an OH hydrogen bond. This claim is rejected by the reviewers of your paper! Although your measurements are correct, what mistake have you ma ...
The Protein Truncation Test
The Protein Truncation Test

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CELL TRANSPORT QUESTIONS 1. (a) Describe a chemical test you

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BBSRC 24/B11662 "Protein processing and electron transfer in

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Protein Supplies for Beef Cattle Diets

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Human lung tissue lysate - soluble fraction (female, 54 years)

... This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphologyspecific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the ...
Heat Shock Proteins and Neurodegenerative Disorders
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... *Next to the second carbohydrate add a series of dots ( . . .) to show that in a real carbohydrate the glucoses would just keep adding on to make a larger polymer. d. Like a fat and a protein, when a carbohydrate is made, water is made again! This time though, 3 molecules of water are made. Make the ...
Mitochondrial Uncoupling Proteins in Mammals and Plants
Mitochondrial Uncoupling Proteins in Mammals and Plants

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Searching for Important Amino Acids in DNA

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A human phenome-interactome network of protein complexes

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CH 5 CQ
CH 5 CQ

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Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
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