Supplementary information - Word file (31 KB )
... 0.2% Tween]) was incubated with GST-Smad4 protein at 4oC for one hour. Beads were precipitated and resuspended in TBST+0.2% BSA, then incubated at 4oC for 10 minutes. This step was repeated and beads were then resuspended in 280l of TBST+0.2% BSA. 20l of this slurry (containing ~1g of GST-Smad4 p ...
... 0.2% Tween]) was incubated with GST-Smad4 protein at 4oC for one hour. Beads were precipitated and resuspended in TBST+0.2% BSA, then incubated at 4oC for 10 minutes. This step was repeated and beads were then resuspended in 280l of TBST+0.2% BSA. 20l of this slurry (containing ~1g of GST-Smad4 p ...
MCB Test 3 Review
... We know the structure factor amplitudes after successful data collection. Unfortunately, conventional x-ray diffraction doesn’t allow for direct phase measurement. This is know as the crystallographic phase problem. ...
... We know the structure factor amplitudes after successful data collection. Unfortunately, conventional x-ray diffraction doesn’t allow for direct phase measurement. This is know as the crystallographic phase problem. ...
Detergent-resistant membranes and the protein
... cholesterol, glycosphingolipids, sphingomyelin and saturated glycerophospholipids [2,21]. These DRM lipids can by themselves form a liquid-ordered-like state in which acyl chains are tightly packed, highly ordered and extended, supporting the raft hypothesis [22]. Extensive lipid analyses have revea ...
... cholesterol, glycosphingolipids, sphingomyelin and saturated glycerophospholipids [2,21]. These DRM lipids can by themselves form a liquid-ordered-like state in which acyl chains are tightly packed, highly ordered and extended, supporting the raft hypothesis [22]. Extensive lipid analyses have revea ...
Exam #3 2 Problem 1. (25 points) You study ligand binding to two
... Carbon monoxide, an odorless gas, binds to hemoglobin to form CO-hemoglobin. Crystals of CO-hemoglobin are isomorphous with those of oxyhemoglobin, which suggests that COHb has the same conformation as oxyhemoglobin. Each heme in Hb can bind one CO molecule, but O2 and CO cannot simultaneously bind ...
... Carbon monoxide, an odorless gas, binds to hemoglobin to form CO-hemoglobin. Crystals of CO-hemoglobin are isomorphous with those of oxyhemoglobin, which suggests that COHb has the same conformation as oxyhemoglobin. Each heme in Hb can bind one CO molecule, but O2 and CO cannot simultaneously bind ...
Control citrus HLB by blocking the functions of two critical effectors
... REPORT UPDATE (500 words-It is not necessary in this public report to disclose your institution's proprietary information or intellectual property.) The objectives of this project are to characterize the molecular interactions between the effectors and the host mitochondrial proteins; to screen for ...
... REPORT UPDATE (500 words-It is not necessary in this public report to disclose your institution's proprietary information or intellectual property.) The objectives of this project are to characterize the molecular interactions between the effectors and the host mitochondrial proteins; to screen for ...
Summary Summary Glutaredoxins (Grx) and Glyoxalases 1 and 2
... and second substrate, and carboxylic acid and GSH as first and second product, respectively). Glutathione is predominantly bound via two conserved basic residues close to the C-terminus of the enzyme. The mechanism not only explains acid-base catalysis, but also other enzyme-features such as unusual ...
... and second substrate, and carboxylic acid and GSH as first and second product, respectively). Glutathione is predominantly bound via two conserved basic residues close to the C-terminus of the enzyme. The mechanism not only explains acid-base catalysis, but also other enzyme-features such as unusual ...
Global Proteomics of the Extremophile Black Fungus Cryomyces
... At the same time the described characteristics of these fungi make it difficult to perform analysis on the cellular level. For instance the cell wall is the main obstacle in protein extraction from fungal biomass. Moreover, due to rigid cell wall and melanin content, also DNA extraction and purifica ...
... At the same time the described characteristics of these fungi make it difficult to perform analysis on the cellular level. For instance the cell wall is the main obstacle in protein extraction from fungal biomass. Moreover, due to rigid cell wall and melanin content, also DNA extraction and purifica ...
ch 6 group review
... 14) A high-protein diet has been linked to a lower risk of heart disease. Page Ref: 204 15) Consuming too much of any one amino acid via supplements can impede absorption of other amino acids in the intestinal tract. Page Ref: 198 16) Vegetarians can only consume adequate protein by eating a variety ...
... 14) A high-protein diet has been linked to a lower risk of heart disease. Page Ref: 204 15) Consuming too much of any one amino acid via supplements can impede absorption of other amino acids in the intestinal tract. Page Ref: 198 16) Vegetarians can only consume adequate protein by eating a variety ...
tuesday_lect_prot_DBs
... Shamelessly copied from material done by Eija Korpelainen This also includes old material from my thesis www.hytti.uku.fi/~toronen/Gradu_verkkoon.zip and from CSC bio-opas http://www.csc.fi/oppaat/bio/ http://www.csc.fi/oppaat/bio/bio-opas.pdf ...
... Shamelessly copied from material done by Eija Korpelainen This also includes old material from my thesis www.hytti.uku.fi/~toronen/Gradu_verkkoon.zip and from CSC bio-opas http://www.csc.fi/oppaat/bio/ http://www.csc.fi/oppaat/bio/bio-opas.pdf ...
Inner nuclear membrane protein transport is mediated by multiple
... went unchallenged. However, with the exception of the correlation between the size of the peripheral channels and the measured diffusion limit, the results supporting lateral diffusion are equally consistent with a translocation mechanism involving vesicle fusion. Vesicle fusion has been extensively ...
... went unchallenged. However, with the exception of the correlation between the size of the peripheral channels and the measured diffusion limit, the results supporting lateral diffusion are equally consistent with a translocation mechanism involving vesicle fusion. Vesicle fusion has been extensively ...
Cell Physiology
... – each oxygen atom accepts two electrons from cytochrome a3 and two protons from the mitochondrial matrix forming water • body’s primary source of metabolic water – water synthesized in the body ...
... – each oxygen atom accepts two electrons from cytochrome a3 and two protons from the mitochondrial matrix forming water • body’s primary source of metabolic water – water synthesized in the body ...
β- Hydroxyacyl-acyl Carrier Protein Dehydratase (FabZ) from
... dehydratase–isomerase of E. coli in unsaturated fatty acid biosynthesis in vivo. FabA was also found to lack isomerase activity in vitro (Zhu et al., 2009). Moynié et al. reported that trans-2-enoyl-ACP polymerized in a progressive manner, and FabA in Pseudomonas aeruginosa underwent a second reacti ...
... dehydratase–isomerase of E. coli in unsaturated fatty acid biosynthesis in vivo. FabA was also found to lack isomerase activity in vitro (Zhu et al., 2009). Moynié et al. reported that trans-2-enoyl-ACP polymerized in a progressive manner, and FabA in Pseudomonas aeruginosa underwent a second reacti ...
Metabolism of erythrocytes
... 50% lipid bilayer (phospholipids, cholesterol) 50% proteins SDS-PAGE: separation of proteins (band 1-7) isolation and analysis (10 main proteins) Integral: Anion exchanger protein, Glycophorin A, B, C Peripheral: Spectrin, Ankyrin, Actin ...
... 50% lipid bilayer (phospholipids, cholesterol) 50% proteins SDS-PAGE: separation of proteins (band 1-7) isolation and analysis (10 main proteins) Integral: Anion exchanger protein, Glycophorin A, B, C Peripheral: Spectrin, Ankyrin, Actin ...
Sex linked inheritance, sex linkage in Drosophila and man, XO, XY
... Amino Acids Are First Activated by ATP and then transfer to tRNA to produce aminoacyl-tRNA (aminoacyl-tRNA synthetase). The activated tRNA is bound in the P site on the ribosome. ...
... Amino Acids Are First Activated by ATP and then transfer to tRNA to produce aminoacyl-tRNA (aminoacyl-tRNA synthetase). The activated tRNA is bound in the P site on the ribosome. ...
Protein synthesis I Biochemistry 302 February 17, 2006
... that binds first to the P site. IF-2 is a G protein (GTPase). 50S subunit then joins the 30S pre-initiation complex. This occurs with simultaneous hydrolysis of GTP and release of IF-1, IF-2, and IF3. ...
... that binds first to the P site. IF-2 is a G protein (GTPase). 50S subunit then joins the 30S pre-initiation complex. This occurs with simultaneous hydrolysis of GTP and release of IF-1, IF-2, and IF3. ...
Final Exam from S06
... the backbone having the 5’ carbon of a purine opposite to a free 3’ carbon of pyrimidine. 2.2 A sexually reproducing animal has two genes on different chromosomes, one for head shape (H) and one for tail length (T). Its genotype is HhTt. Which of the following genotypes is possible in a gamete (meio ...
... the backbone having the 5’ carbon of a purine opposite to a free 3’ carbon of pyrimidine. 2.2 A sexually reproducing animal has two genes on different chromosomes, one for head shape (H) and one for tail length (T). Its genotype is HhTt. Which of the following genotypes is possible in a gamete (meio ...
Protein kinase Protein kinases are enzymes that add a phosphate
... at more than one residue by a given protein kinase, and many proteins can be phosphorylated by several protein kinases. There are more genes encoding protein kinases than there are genes encoding proteins for any other family of human enzymes except proteases. Workers have identified 478 typical an ...
... at more than one residue by a given protein kinase, and many proteins can be phosphorylated by several protein kinases. There are more genes encoding protein kinases than there are genes encoding proteins for any other family of human enzymes except proteases. Workers have identified 478 typical an ...
Gel Electrophoresis of DNA
... gel is run. The time of the run depends upon the amount of current and % gel, and requires experimentation • At the end of the run the gel is removed (it is actually quite stiff) • The gel is then visualized - UV light causes the bands of DNA to fluoresce ...
... gel is run. The time of the run depends upon the amount of current and % gel, and requires experimentation • At the end of the run the gel is removed (it is actually quite stiff) • The gel is then visualized - UV light causes the bands of DNA to fluoresce ...
How do ribosomes hold tRNA and mRNA together
... P sites are tRNA being bound to a peptide that is being synthesized. A sites are tRNA that is bound to an amino acid. E sites bounds a free tRNA before it leaves the ribosome. Are they found in prokaryotic cells? Yes. What is cytosol? It is the fluid inside of the cell. What exactly do Ribosomes do? ...
... P sites are tRNA being bound to a peptide that is being synthesized. A sites are tRNA that is bound to an amino acid. E sites bounds a free tRNA before it leaves the ribosome. Are they found in prokaryotic cells? Yes. What is cytosol? It is the fluid inside of the cell. What exactly do Ribosomes do? ...
Bioinformatics Analysis of Phenylacetaldehyde Synthase (PAAS), a
... proteins were predicted by using the method of preference functions (http://split.pmfst.hr/split/4). The predicted TM helix position of PhPAAS was 108-131 amino acids, however this trends was 251265 in RhPAAS. Transmembrane domain usually denotes a single transmembrane α-helix of a transmembrane pro ...
... proteins were predicted by using the method of preference functions (http://split.pmfst.hr/split/4). The predicted TM helix position of PhPAAS was 108-131 amino acids, however this trends was 251265 in RhPAAS. Transmembrane domain usually denotes a single transmembrane α-helix of a transmembrane pro ...
Chapter 2 part 3
... – Proteins are also enzymes that speed up the chemical reactions of metabolism. – Proteins such as hemoglobin are responsible for the transport of substances within the body. – Proteins also transport substances across cell membranes. ...
... – Proteins are also enzymes that speed up the chemical reactions of metabolism. – Proteins such as hemoglobin are responsible for the transport of substances within the body. – Proteins also transport substances across cell membranes. ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.