CHM 365 Name: Exam 2 Oct. 13, 2004 Do all of the questions. Part I
... All of the following statements are true about the relationships between [S], Km and Vmax EXCEPT: a) As the [S] is increased, v approaches the limiting value, Vmax. b) Km = Vmax /2. c) The rate of the reaction, v, follows a first order rate equation v = K'[A] and K' = Vmax/K m. d) The rate of produc ...
... All of the following statements are true about the relationships between [S], Km and Vmax EXCEPT: a) As the [S] is increased, v approaches the limiting value, Vmax. b) Km = Vmax /2. c) The rate of the reaction, v, follows a first order rate equation v = K'[A] and K' = Vmax/K m. d) The rate of produc ...
Bacterial enzymes that can deglycate glucose
... of the Schiff base resulting from the condensation of fructose 6phosphate and ammonia. Both glucoselysine and fructoselysine are compounds that are abundantly present in free form in vegetable and fruits, where their concentration may reach approx. 7 % of the fresh mass, i.e. 400 mM [15]. Thus it is ...
... of the Schiff base resulting from the condensation of fructose 6phosphate and ammonia. Both glucoselysine and fructoselysine are compounds that are abundantly present in free form in vegetable and fruits, where their concentration may reach approx. 7 % of the fresh mass, i.e. 400 mM [15]. Thus it is ...
Basics of BLAST - GEP Community Server
... • GenBank NR (protein and nucleotide versions) - Non-redundant large databases (compile and remove duplicates) - Anyone can submit, you can call your sequence anything - Low quality; names can be meaningless • EST databases - Short single reads of cDNA clones - Short single reads - High error rates ...
... • GenBank NR (protein and nucleotide versions) - Non-redundant large databases (compile and remove duplicates) - Anyone can submit, you can call your sequence anything - Low quality; names can be meaningless • EST databases - Short single reads of cDNA clones - Short single reads - High error rates ...
Supplementary Table VI. List of Deletions/Hypomorphic Alleles
... member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and l ...
... member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and l ...
6th Grade Organic Compounds
... Cells build biomolecules by bonding small molecules together to form chains ...
... Cells build biomolecules by bonding small molecules together to form chains ...
Oligomerization and activation of the FliI ATPase
... ATPase, and the lipid and protein interactions that locate and regulate it. A previous report has indicated that His-tagged FliI can exist in monomeric form (Minamino and Macnab, 2000a), and our gel filtration and multiangle light scattering analysis of the native soluble enzyme confirmed this earli ...
... ATPase, and the lipid and protein interactions that locate and regulate it. A previous report has indicated that His-tagged FliI can exist in monomeric form (Minamino and Macnab, 2000a), and our gel filtration and multiangle light scattering analysis of the native soluble enzyme confirmed this earli ...
Lecture 10 - Protein Turnover and Amino Acid
... 1.2 Cellular Protein Degradation Cellular proteins are degraded at different rates. Ornithine decarboxylase has a half-life of 11 minutes. Hemoglobin lasts as long as a red blood cell. Υ-Crystallin (eye lens protein) lasts as long as the organism does. ...
... 1.2 Cellular Protein Degradation Cellular proteins are degraded at different rates. Ornithine decarboxylase has a half-life of 11 minutes. Hemoglobin lasts as long as a red blood cell. Υ-Crystallin (eye lens protein) lasts as long as the organism does. ...
Protein Synthesis Worksheet
... 1. In DNA, adenine binds with ____________ and guanine binds with _____________. 2. In RNA, adenine binds with ____________ and guanine binds with _____________. 3. Transcription takes place in the ________________; translation takes place in the _______________. 4. The building blocks of nucleic ac ...
... 1. In DNA, adenine binds with ____________ and guanine binds with _____________. 2. In RNA, adenine binds with ____________ and guanine binds with _____________. 3. Transcription takes place in the ________________; translation takes place in the _______________. 4. The building blocks of nucleic ac ...
Rabphilin mutants defective for Rab3 binding
... Partitioning of full-length Rabphilin mutants in cell extracts PC12 cell fractions were prepared as described (McKiernan et al., 1996). HIT-T15 cell fractions were prepared in a similar manner, after lysing the cells by sonication for 1 second. After SDS-PAGE and transfer on a nitrocellulose membran ...
... Partitioning of full-length Rabphilin mutants in cell extracts PC12 cell fractions were prepared as described (McKiernan et al., 1996). HIT-T15 cell fractions were prepared in a similar manner, after lysing the cells by sonication for 1 second. After SDS-PAGE and transfer on a nitrocellulose membran ...
Enzyme Structure and Function
... sold an average of 2 per month in 1997 but sell an average of 25 per month in 2007) – Just like, with enzymes, the rate of the reaction increases. ...
... sold an average of 2 per month in 1997 but sell an average of 25 per month in 2007) – Just like, with enzymes, the rate of the reaction increases. ...
Gene Cloning, Expression, and Substrate Specificity of an Imidase
... HindIII, was inserted into the corresponding sites of the modified vector pET32a to form recombinant plasmid pEI. Expression and purification of imidase. Recombinant plasmid was transformed into E. coli BL21 (DE3) host cells for large-scale protein production. The host cells containing the recombina ...
... HindIII, was inserted into the corresponding sites of the modified vector pET32a to form recombinant plasmid pEI. Expression and purification of imidase. Recombinant plasmid was transformed into E. coli BL21 (DE3) host cells for large-scale protein production. The host cells containing the recombina ...
MULTIPLE CHOICE. Choose the one alternative that best completes
... B) The compositions of the intracellular fluid and extracellular fluid are identical due to the free movement of molecules across the cell membrane. C) The Golgi apparatus contains the enzyme catalase to break down the hydrogen peroxide it produces when degrading oxygen-derived wastes. D) Proteins t ...
... B) The compositions of the intracellular fluid and extracellular fluid are identical due to the free movement of molecules across the cell membrane. C) The Golgi apparatus contains the enzyme catalase to break down the hydrogen peroxide it produces when degrading oxygen-derived wastes. D) Proteins t ...
protein folding - Federation of American Societies for Experimental
... the process. This help consists of proteins called chaperones (or chaperonins) that are associated with the target protein during part of its folding process. However, once folding is complete (or even before) the chaperone will leave its current protein molecule and go on to support the folding of ...
... the process. This help consists of proteins called chaperones (or chaperonins) that are associated with the target protein during part of its folding process. However, once folding is complete (or even before) the chaperone will leave its current protein molecule and go on to support the folding of ...
Proportion of animal protein Consumption
... Some of the animal-derived dietary proteins are much more positive in their δ13C values than those from plant sources. ...
... Some of the animal-derived dietary proteins are much more positive in their δ13C values than those from plant sources. ...
NMR-driven secondary and tertiary structure model of Ca
... (Fig. 4). Both of the fingerprint regions also contain acidic residues, which, in other EF-hand proteins, are known to interact with basic residues on the target [16]. Lastly, the fingerprint region within EF-III contains one or more Met residues, which may also be important in target interaction and ...
... (Fig. 4). Both of the fingerprint regions also contain acidic residues, which, in other EF-hand proteins, are known to interact with basic residues on the target [16]. Lastly, the fingerprint region within EF-III contains one or more Met residues, which may also be important in target interaction and ...
The Proteins of the Keratin Component of Bird`s Beaks
... form, 0·1 % ~-mercaptoethanol was included in the electrode buffer and sample solution.
... form, 0·1 % ~-mercaptoethanol was included in the electrode buffer and sample solution.
N D - Wiley
... The denatured state heat capacity, CpD, was approximated for simplicity by a linear function as in the work of Viguera and co-authors [1], even though a binomial behavior of CpD has been defined for proteins [2,3]. It was found that linear approximation of the CpD does not significantly affect the t ...
... The denatured state heat capacity, CpD, was approximated for simplicity by a linear function as in the work of Viguera and co-authors [1], even though a binomial behavior of CpD has been defined for proteins [2,3]. It was found that linear approximation of the CpD does not significantly affect the t ...
please click, ppt - Department of Statistics | Rajshahi University
... lymphoreticular system and likely from other sites prions proceed along the peripheral nervous system to finally reach the brain, either directly via the vagus nerve or via the spinal cord, under involvement of the sympathetic nervous system. ...
... lymphoreticular system and likely from other sites prions proceed along the peripheral nervous system to finally reach the brain, either directly via the vagus nerve or via the spinal cord, under involvement of the sympathetic nervous system. ...
Protein Structure Prediction and Structural Genomics
... from focusing on only novel folds to selecting all proteins in a model genome. A modelcentric view requires that targets be selected such that most of the remaining sequences can be modeled with useful accuracy by comparative modeling. Even with structural genomics, the structure of most of the prot ...
... from focusing on only novel folds to selecting all proteins in a model genome. A modelcentric view requires that targets be selected such that most of the remaining sequences can be modeled with useful accuracy by comparative modeling. Even with structural genomics, the structure of most of the prot ...
Gene regulation in bacteria -
... more complex than suggested above and the promoter is controlled by two different regulatory molecules. In response to low levels of its preferred energy source (glucose), E. coli produces a molecule called cyclic AMP (cAMP). This signal molecule interacts with the Cyclic AMP Receptor Protein (CRP) ...
... more complex than suggested above and the promoter is controlled by two different regulatory molecules. In response to low levels of its preferred energy source (glucose), E. coli produces a molecule called cyclic AMP (cAMP). This signal molecule interacts with the Cyclic AMP Receptor Protein (CRP) ...
Exercises in MBV-INF 4410/9410/9410A
... (MUTYH) (546 aa), N-glycosylase/DNA lyase isoform 1a (OGG1) (345 aa) and methylCpG-binding domain protein 4 (MBD4) (580 aa). The MBD4 protein may have an E-value worse than the PSI-BLAST threshold, but is still a homolog. Give the sequences short names. b) Make a multiple sequence alignment of the f ...
... (MUTYH) (546 aa), N-glycosylase/DNA lyase isoform 1a (OGG1) (345 aa) and methylCpG-binding domain protein 4 (MBD4) (580 aa). The MBD4 protein may have an E-value worse than the PSI-BLAST threshold, but is still a homolog. Give the sequences short names. b) Make a multiple sequence alignment of the f ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.