Protein synthesis
... within the proteins themselves. These sequences can be responsible for proteins being secreted, imported into the nucleus or targeted to other organelles. ...
... within the proteins themselves. These sequences can be responsible for proteins being secreted, imported into the nucleus or targeted to other organelles. ...
MedBiochem Exam For each of the following questions, choose the
... E. amytal 34. Oligomycin interferes with synthesis of "high energy" compounds by A. blocking the transfer of electrons from cytochrome b to cytochrome c. B. uncoupling electron transport from oxidative phosphorylation. C. closing the proton channel through the stalk of ATP synthetase. D. inhibiting ...
... E. amytal 34. Oligomycin interferes with synthesis of "high energy" compounds by A. blocking the transfer of electrons from cytochrome b to cytochrome c. B. uncoupling electron transport from oxidative phosphorylation. C. closing the proton channel through the stalk of ATP synthetase. D. inhibiting ...
Degradation by bacteria of membrane and soluble protein in seawater
... extract was added to the solution of Pronase E (Sigma; 500 mg ml-' dissolved in ASW),incubated for 30 mln at 22°C. The protease-treated membrane fraction was then washed twice and collected by ultracentrifugation (55000 X g, 1 h). Biochemical characterization of radiolabeled preparations. To examine ...
... extract was added to the solution of Pronase E (Sigma; 500 mg ml-' dissolved in ASW),incubated for 30 mln at 22°C. The protease-treated membrane fraction was then washed twice and collected by ultracentrifugation (55000 X g, 1 h). Biochemical characterization of radiolabeled preparations. To examine ...
Process Biochemistry
... process is based on extracting muscle protein at pH 2–3 or 11–12, followed by iso-electric precipitation. Precipitated protein is then collected by centrifugation to obtain the protein isolate. In most of those studies, researchers successfully used fresh raw material [7– 16]. In our study, the proc ...
... process is based on extracting muscle protein at pH 2–3 or 11–12, followed by iso-electric precipitation. Precipitated protein is then collected by centrifugation to obtain the protein isolate. In most of those studies, researchers successfully used fresh raw material [7– 16]. In our study, the proc ...
What is NPN in feed, How does it work
... that often occurs when cattle are started on urea supplements. Don’t feed urea supplements to shipped cattle that have been off feed for two or three days. Urea should not supply more than one third of the protein equivalent in the total ration. Cattle on moderate-energy growing rations should limit ...
... that often occurs when cattle are started on urea supplements. Don’t feed urea supplements to shipped cattle that have been off feed for two or three days. Urea should not supply more than one third of the protein equivalent in the total ration. Cattle on moderate-energy growing rations should limit ...
Aspekte der Thermodynamik in der Strukturbiologie Einführung in
... • Bioinformatics = computational branch of molecular biology • in vivo – in vitro – in silico • Bioinformatics in a narrower sense: Databases and computational methods for sequences and sequence-related properties of proteins, DNA, and RNA ...
... • Bioinformatics = computational branch of molecular biology • in vivo – in vitro – in silico • Bioinformatics in a narrower sense: Databases and computational methods for sequences and sequence-related properties of proteins, DNA, and RNA ...
Slide 1
... A, E, M common in -helices N: residues in all protein structures M: residues in -helices Y: Total Ala in protein structures X: Ala in -helices Propensity Ala in -helix: (X/Y)/(M/N) ...
... A, E, M common in -helices N: residues in all protein structures M: residues in -helices Y: Total Ala in protein structures X: Ala in -helices Propensity Ala in -helix: (X/Y)/(M/N) ...
Section 2C Addition of an Epitope Tag Sequence to a Target Gene
... © One oligonucleotide contains the complete coding sequence for the epitope tag; the other oligonucleotide contains nucleotides that will hybridize to that coding sequence Caution: For the coding sequence, choose codons that are most likely to be used in the organism where the target protein will be ...
... © One oligonucleotide contains the complete coding sequence for the epitope tag; the other oligonucleotide contains nucleotides that will hybridize to that coding sequence Caution: For the coding sequence, choose codons that are most likely to be used in the organism where the target protein will be ...
Improved detection and identification of low
... over 20 h. IPG strips can also be run on IPGphor™ electrophoresis unit using paper bridges on Cup Loading Strip Holder, which achieves equally high spot resolution. All chemicals and reagents used for the second dimension of 2-D electrophoresis are described in reference 6. Initial equilibration of ...
... over 20 h. IPG strips can also be run on IPGphor™ electrophoresis unit using paper bridges on Cup Loading Strip Holder, which achieves equally high spot resolution. All chemicals and reagents used for the second dimension of 2-D electrophoresis are described in reference 6. Initial equilibration of ...
Pinar Tulay cell molecules_17
... • The principal storage polysaccharides are starch (in plant cells) and glycogen (in animal cells), both of which are composed of many glucose units. • Both starch and glycogen constitute good storage products because the bonds linking the glucose molecules are easily ...
... • The principal storage polysaccharides are starch (in plant cells) and glycogen (in animal cells), both of which are composed of many glucose units. • Both starch and glycogen constitute good storage products because the bonds linking the glucose molecules are easily ...
Identification of a novel human ... through its interaction with the ...
... polyclonal anti-Grb14 antibody (Santa Cruz Biotechnology, Santa Cruz, CA), D8 polyclonal anti-Flag antibody (Santa Cruz Biotechnology). ...
... polyclonal anti-Grb14 antibody (Santa Cruz Biotechnology, Santa Cruz, CA), D8 polyclonal anti-Flag antibody (Santa Cruz Biotechnology). ...
How to visually interpret biological data using networks
... that work together to control essential functions17. Similarity relationships. These link biological objects that are similar according to a common attribute. Many different similarity measures can be used, such as protein sequence similarity or gene coexpression based on correlated transcriptional ...
... that work together to control essential functions17. Similarity relationships. These link biological objects that are similar according to a common attribute. Many different similarity measures can be used, such as protein sequence similarity or gene coexpression based on correlated transcriptional ...
slides
... These methods were based on the helix- or sheet-forming propensities of individual amino acids, sometimes coupled with rules for estimating the free energy of forming secondary structure elements. Such methods were typically ~60% accurate in predicting which of the three states (helix/sheet/coil) a ...
... These methods were based on the helix- or sheet-forming propensities of individual amino acids, sometimes coupled with rules for estimating the free energy of forming secondary structure elements. Such methods were typically ~60% accurate in predicting which of the three states (helix/sheet/coil) a ...
Griffith_155
... gives a baseline reading. PI-PLC activity is then eluted with the same buffer containing 50% ethylene glycol (by volume). Fractions containing PI-PLC activity are pooled, and the ethylene glycol is removed and the solution concentrated by ultrafiltration. Enzyme solutions are stored in 20 mM Tris-HC ...
... gives a baseline reading. PI-PLC activity is then eluted with the same buffer containing 50% ethylene glycol (by volume). Fractions containing PI-PLC activity are pooled, and the ethylene glycol is removed and the solution concentrated by ultrafiltration. Enzyme solutions are stored in 20 mM Tris-HC ...
Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
... acids with substituted stable isotopic nuclei (e.g. 13C, 15N). Thus, in an experiment, two cell populations are grown in culture media that are identical except that one of them contains a “light” and the other a “heavy” form of a particular amino acid (e.g. 12C and ...
... acids with substituted stable isotopic nuclei (e.g. 13C, 15N). Thus, in an experiment, two cell populations are grown in culture media that are identical except that one of them contains a “light” and the other a “heavy” form of a particular amino acid (e.g. 12C and ...
ch4 FA 11 - Cal State LA
... The Plasma Membrane • Facilitated diffusion – Transporter catalyzes movement from one side to other, driven by movement down concentration gradient – 1st step of glycolysis lowers cytoplasmic concentration – Can be regulated ...
... The Plasma Membrane • Facilitated diffusion – Transporter catalyzes movement from one side to other, driven by movement down concentration gradient – 1st step of glycolysis lowers cytoplasmic concentration – Can be regulated ...
The HSSP database of protein structure–sequence
... vary considerably while maintaining the same overall 3-D structure. One can therefore group sequence-similar proteins into families of structural homologues. If the 3-D structure of only one family member is known, then by implication one can derive the basic 3-D structure, or fold, of all family me ...
... vary considerably while maintaining the same overall 3-D structure. One can therefore group sequence-similar proteins into families of structural homologues. If the 3-D structure of only one family member is known, then by implication one can derive the basic 3-D structure, or fold, of all family me ...
Determination of the Binding Site-Size of the Protein
... percent of polyacrylamide gel will be considered for the bigger protein. Polyacrylamide gels, usually in 6-12% w/v, are made using an acrylamide to bisacrylamide weight ratio of 19:1 and TBE as the buffer in the gel and for electrophoresis. Electrophoresis buffers. The process of electrophoretic sep ...
... percent of polyacrylamide gel will be considered for the bigger protein. Polyacrylamide gels, usually in 6-12% w/v, are made using an acrylamide to bisacrylamide weight ratio of 19:1 and TBE as the buffer in the gel and for electrophoresis. Electrophoresis buffers. The process of electrophoretic sep ...
Nitrogen Metabolism
... • Proteins have different biological value (BV) • Major reasons why animal protein is called BV protein, whereas plant protein is of lower BV: – Animal protein is “complete” - contains all essential amino acids – Contains essential amino acids in larger amounts and in proper ...
... • Proteins have different biological value (BV) • Major reasons why animal protein is called BV protein, whereas plant protein is of lower BV: – Animal protein is “complete” - contains all essential amino acids – Contains essential amino acids in larger amounts and in proper ...
Cell Organisation
... • Contains pore complexes for shuttling of proteins, ribosomes and RNA: ribosomes and RNA produced in nucleus, must shuttle to cytoplasm for protein synthesis, some proteins (i.e. transcription factors) must shuttle back to nucleus ...
... • Contains pore complexes for shuttling of proteins, ribosomes and RNA: ribosomes and RNA produced in nucleus, must shuttle to cytoplasm for protein synthesis, some proteins (i.e. transcription factors) must shuttle back to nucleus ...
22. Analysis of Haloarchaeal Genomes
... ● produces pentoses for RNA, DNA synthesis ● produces NADPH for biosynthesis of amino acids, etc. ...
... ● produces pentoses for RNA, DNA synthesis ● produces NADPH for biosynthesis of amino acids, etc. ...
A Rab4-like GTPase in Dictyostelium discoideum
... Relative amounts of RabD were estimated by comparison to a standard curve generated using known amounts (0.01-1.0 µg) of the recombinant RabD antigen subjected to western blot analysis. Densitometric scans of photographic negatives of the blots indicated that the signal was linear over a 50-fold ran ...
... Relative amounts of RabD were estimated by comparison to a standard curve generated using known amounts (0.01-1.0 µg) of the recombinant RabD antigen subjected to western blot analysis. Densitometric scans of photographic negatives of the blots indicated that the signal was linear over a 50-fold ran ...
Supplementary information - Word file (31 KB )
... 0.2% Tween]) was incubated with GST-Smad4 protein at 4oC for one hour. Beads were precipitated and resuspended in TBST+0.2% BSA, then incubated at 4oC for 10 minutes. This step was repeated and beads were then resuspended in 280l of TBST+0.2% BSA. 20l of this slurry (containing ~1g of GST-Smad4 p ...
... 0.2% Tween]) was incubated with GST-Smad4 protein at 4oC for one hour. Beads were precipitated and resuspended in TBST+0.2% BSA, then incubated at 4oC for 10 minutes. This step was repeated and beads were then resuspended in 280l of TBST+0.2% BSA. 20l of this slurry (containing ~1g of GST-Smad4 p ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.