Glutathionylation in the Photosynthetic Model Organism
... culture was grown in 100 ml of TAP medium to 6 – 8 ⫻ 106 cells/ml. After centrifugation (4,000 ⫻ g, 3 min), the pellet was washed once with fresh TAP medium and resuspended to 8 ⫻ 106 cells/ml in TAP medium supplemented with BioGEE or BioGSSG (final concentration, 0.5–2 mM). After different incubati ...
... culture was grown in 100 ml of TAP medium to 6 – 8 ⫻ 106 cells/ml. After centrifugation (4,000 ⫻ g, 3 min), the pellet was washed once with fresh TAP medium and resuspended to 8 ⫻ 106 cells/ml in TAP medium supplemented with BioGEE or BioGSSG (final concentration, 0.5–2 mM). After different incubati ...
Chapter 9 Notes - Get a Clue with Mrs. Perdue
... A. __Optimum temperature___ i. greatest number of collisions between enzyme & substrate ii. human enzymes = 35°- 40°C (_body temp = 37 ̊ C_) B. ...
... A. __Optimum temperature___ i. greatest number of collisions between enzyme & substrate ii. human enzymes = 35°- 40°C (_body temp = 37 ̊ C_) B. ...
humanbiolecture2
... •Messenger RNA passes through the nuclear membrane to bring the genetic information to either ribosomes or rough endoplasmic reticulum for protein synthesis •At the ribosome, transfer RNA matches the messenger RNA code and brings amino acids, which become linked by dehydration synthesis to form prot ...
... •Messenger RNA passes through the nuclear membrane to bring the genetic information to either ribosomes or rough endoplasmic reticulum for protein synthesis •At the ribosome, transfer RNA matches the messenger RNA code and brings amino acids, which become linked by dehydration synthesis to form prot ...
marker - Cat`s TCM Notes
... in 'window period' after surface antigen disappears and before Two classes: anti-HBc IgM, and anti-HBc surface antibody appears. IgG also known as HBcAb or 'core antibody' HBeAg (water-soluble form of core Detectable in early phase of hepatitis B virus infection, shortly protein - secreted by cells ...
... in 'window period' after surface antigen disappears and before Two classes: anti-HBc IgM, and anti-HBc surface antibody appears. IgG also known as HBcAb or 'core antibody' HBeAg (water-soluble form of core Detectable in early phase of hepatitis B virus infection, shortly protein - secreted by cells ...
Investigation of the starch-binding properties of wheat friabilin
... 15kDa (under reducing conditions). The molecular weight calculated from the DNA sequences is 14kDa for both PA and GSPP. The difference between the calculated and apparent molecular weights of PA and GSPP might be caused by the large number of basic amino acid residues present in these polypeptides. ...
... 15kDa (under reducing conditions). The molecular weight calculated from the DNA sequences is 14kDa for both PA and GSPP. The difference between the calculated and apparent molecular weights of PA and GSPP might be caused by the large number of basic amino acid residues present in these polypeptides. ...
Drosophila melanogaster
... structurally very different and are taxonomically unrelated, but their similar effects and potential common myosin binding site in the different muscles in which they are expressed might signal their functional homology. Previous work in the Vigoreaux lab developed a series of transgenic D. melanoga ...
... structurally very different and are taxonomically unrelated, but their similar effects and potential common myosin binding site in the different muscles in which they are expressed might signal their functional homology. Previous work in the Vigoreaux lab developed a series of transgenic D. melanoga ...
Unit 2 Student Guided Notes Introduction Carbon is the basic
... acid and an Oxygen further down the chain. An alpha helix contains 3.6 amino acids per spiral. There are other secondary structures, but the alpha helix is the most common and the one you will need to know for this course. Protein Structure - Tertiary and Quaternary Structures The third level is des ...
... acid and an Oxygen further down the chain. An alpha helix contains 3.6 amino acids per spiral. There are other secondary structures, but the alpha helix is the most common and the one you will need to know for this course. Protein Structure - Tertiary and Quaternary Structures The third level is des ...
Anti-beta-Galactosidase, Rabbit IgG Fraction
... The Escherichia coli gene lacZ encoding β-galactosidase is an important reporter gene for detecting the expression of recombinant genes in animal cells. In situations where enzymatic detection of β-galactosidase is not practical, anti–β-galactosidase antibody has been widely used. Anti–β-galactosida ...
... The Escherichia coli gene lacZ encoding β-galactosidase is an important reporter gene for detecting the expression of recombinant genes in animal cells. In situations where enzymatic detection of β-galactosidase is not practical, anti–β-galactosidase antibody has been widely used. Anti–β-galactosida ...
Macromolecules Unit Study Guide
... 12. What are the elements that carbohydrates are made of? Carbon, hydrogen and oxygen 13. What are the monomers of carbohydrates? monosaccharides 14. What are the polymers of carbohydrates? polysaccharides 15. Give some examples of monosaccharides: glucose, galactose 16. Give some examples of polysa ...
... 12. What are the elements that carbohydrates are made of? Carbon, hydrogen and oxygen 13. What are the monomers of carbohydrates? monosaccharides 14. What are the polymers of carbohydrates? polysaccharides 15. Give some examples of monosaccharides: glucose, galactose 16. Give some examples of polysa ...
Tyrosinase Related Protein 75 (TRP75 / gp75) Ab-1
... This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly invest ...
... This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly invest ...
The Structure of a Prokaryotic Cell
... surface Not for locomotion Adhesion (surfaces, cells) ...
... surface Not for locomotion Adhesion (surfaces, cells) ...
Atomistic modeling of the structural components of the
... Russia Olga Grishina, Sararov State University, Russia Michail Slepchenkov, Sararov State University, Russia ...
... Russia Olga Grishina, Sararov State University, Russia Michail Slepchenkov, Sararov State University, Russia ...
Identification of the chlB Gene and the Gene Product Essential for
... at 4°C to remove cell debris. Soluble and insoluble membrane fractions were prepared by ultracentrifugation at 180,000 x g for 1 h. Proteins separated by SDS-PAGE were electro-transferred from the gel to a PVDF membrane (Immobilon P; Millipore, Bedford, MA, U.S.A.) as described by Fujita et al. (198 ...
... at 4°C to remove cell debris. Soluble and insoluble membrane fractions were prepared by ultracentrifugation at 180,000 x g for 1 h. Proteins separated by SDS-PAGE were electro-transferred from the gel to a PVDF membrane (Immobilon P; Millipore, Bedford, MA, U.S.A.) as described by Fujita et al. (198 ...
PASS MOCK EXAM
... b. Phospholipids favor flip motion rather than lateral motion c. Cholesterol is equally distributed over both sides d. Lipid self-assembly is an enzyme catalyzed process 34. Which class of membrane protein is incorrectly matched? a. Peripheral à can only be moved with detergent b. Lipid-ancho ...
... b. Phospholipids favor flip motion rather than lateral motion c. Cholesterol is equally distributed over both sides d. Lipid self-assembly is an enzyme catalyzed process 34. Which class of membrane protein is incorrectly matched? a. Peripheral à can only be moved with detergent b. Lipid-ancho ...
Bone building: perfect protein
... bones, this is built up with a special protein called osteocalcin (OC). It is a small protein, 49 amino acids long (5.8 kDa), and is ‘highly conserved’, meaning that its sequence is almost identical among vertebrates. Human OC has the sequence Tyr-Leu-Tyr-Gln-Trp-LeuGly-Ala-Pro-Val-Pro-TyrPro-Asp-Pr ...
... bones, this is built up with a special protein called osteocalcin (OC). It is a small protein, 49 amino acids long (5.8 kDa), and is ‘highly conserved’, meaning that its sequence is almost identical among vertebrates. Human OC has the sequence Tyr-Leu-Tyr-Gln-Trp-LeuGly-Ala-Pro-Val-Pro-TyrPro-Asp-Pr ...
Text (Acepted version abstract)
... rheumatoid arthritis patients (RA). Predicting response to anti-TNF drugs at baseline remains an elusive goal in RA management. The purpose of this study was to determine if baseline levels of circulating cytokines, soluble receptors, adhesion molecules and metabolic factors differentiate future res ...
... rheumatoid arthritis patients (RA). Predicting response to anti-TNF drugs at baseline remains an elusive goal in RA management. The purpose of this study was to determine if baseline levels of circulating cytokines, soluble receptors, adhesion molecules and metabolic factors differentiate future res ...
Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway
... transfection and washed twice with an ice cold PBS (phosphate-buffered saline) and then lysed on ice for 30 min in lysis buffer (Cell Signalling Technology, USA) supplemented with cocktail tablets and 0.1 mM PMSF with gentle shaking. The solution was then centrifuged at 12,000 g for 30 min at 4 °C t ...
... transfection and washed twice with an ice cold PBS (phosphate-buffered saline) and then lysed on ice for 30 min in lysis buffer (Cell Signalling Technology, USA) supplemented with cocktail tablets and 0.1 mM PMSF with gentle shaking. The solution was then centrifuged at 12,000 g for 30 min at 4 °C t ...
Amino Acids and Healthy Muscle - SEA
... promotion of our body muscles. In particular, the Branched Chain Amino Acids (BCAAs) which cannot be produced in our living body are called “essential amino acids”. Human beings should take BCAAs (valine, leucine and isoleucine) through meal (and / or supplement if needed) in considering the balance ...
... promotion of our body muscles. In particular, the Branched Chain Amino Acids (BCAAs) which cannot be produced in our living body are called “essential amino acids”. Human beings should take BCAAs (valine, leucine and isoleucine) through meal (and / or supplement if needed) in considering the balance ...
Constitutive expression of Vitis vinifera thaumatin
... SDS–PAGE with 1-mm-thick mini gels was carried out as described by Laemmli (1970). Proteins were diluted with an equal volume of SDS–PAGE buffer (Sigma, St Louis, MO) and diluted samples were boiled for 5 min and then cooled. Samples were centrifuged at 5590 g for 5 min at room temperature to remove ...
... SDS–PAGE with 1-mm-thick mini gels was carried out as described by Laemmli (1970). Proteins were diluted with an equal volume of SDS–PAGE buffer (Sigma, St Louis, MO) and diluted samples were boiled for 5 min and then cooled. Samples were centrifuged at 5590 g for 5 min at room temperature to remove ...
JSB-302
... The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the JSB-302 is 150 volts. In a 1% TBE gel, this translates into a run time of approximately 1 hour. Lower voltages may be used, of course, and as a general rule, a 75 volt run will take twice as long as a 150 ...
... The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the JSB-302 is 150 volts. In a 1% TBE gel, this translates into a run time of approximately 1 hour. Lower voltages may be used, of course, and as a general rule, a 75 volt run will take twice as long as a 150 ...
biochem 37 [4-20
... Transport defect of neutral AAs (Iso, Leu, Phe, Thr, Try, & Val) in both intestines and renal tubules i. system B0 [Nupnup, mmm….turkey--tryptophan] ...
... Transport defect of neutral AAs (Iso, Leu, Phe, Thr, Try, & Val) in both intestines and renal tubules i. system B0 [Nupnup, mmm….turkey--tryptophan] ...
Model Description Sheet
... neurotoxin, causing muscle paralysis in the host by blocking the release of the neurotransmitter, acetylcholine, from motor neurons associated with skeletal muscle. Despite this toxicity, BoNT/A is used pharmaceutically as a treatment for numerous neurological diseases, including migraines, dystonia ...
... neurotoxin, causing muscle paralysis in the host by blocking the release of the neurotransmitter, acetylcholine, from motor neurons associated with skeletal muscle. Despite this toxicity, BoNT/A is used pharmaceutically as a treatment for numerous neurological diseases, including migraines, dystonia ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.