Introduction
... GR Safe I is a new, safe nucleic acid stain for detecting double-stranded DNA, single-stranded DNA, and RNA in agarose gel. It can be used for replacing mutagenic ethidium bromide (EB). GR Safe emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This new sta ...
... GR Safe I is a new, safe nucleic acid stain for detecting double-stranded DNA, single-stranded DNA, and RNA in agarose gel. It can be used for replacing mutagenic ethidium bromide (EB). GR Safe emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This new sta ...
Chapter 5: The Structure and Function of Macromolecules
... 7. Which one of the following carbohydrate molecules has the lowest molecular weight? (Concept 5.2 ) a) sucrose b) lactose c) glucose d) cellulose e) chitin 8. Amylase is an enzyme that breaks down starch. Why can't the same enzyme break down cellulose? (Concept 5.2 ) a) The enzyme cannot attack cel ...
... 7. Which one of the following carbohydrate molecules has the lowest molecular weight? (Concept 5.2 ) a) sucrose b) lactose c) glucose d) cellulose e) chitin 8. Amylase is an enzyme that breaks down starch. Why can't the same enzyme break down cellulose? (Concept 5.2 ) a) The enzyme cannot attack cel ...
Protein Folding Activity
... Amino acids are small molecules that link together in long chains to form proteins. They are often referred to as the "building blocks" of proteins. The sequence of amino acids in a protein, and hence the protein function, are determined by the genetic code, defined by the sequence of nucleotides in ...
... Amino acids are small molecules that link together in long chains to form proteins. They are often referred to as the "building blocks" of proteins. The sequence of amino acids in a protein, and hence the protein function, are determined by the genetic code, defined by the sequence of nucleotides in ...
Slide 1
... • At least two genes are directly controlled by CsgD: csgBA (curli subunits) and adrA (cellulose) • Genomic and proteomic approach comparing csgDproficient to strains in which no csgD expression is detectable to identify novel csgD-dependent genes ...
... • At least two genes are directly controlled by CsgD: csgBA (curli subunits) and adrA (cellulose) • Genomic and proteomic approach comparing csgDproficient to strains in which no csgD expression is detectable to identify novel csgD-dependent genes ...
Protein Crystallization
... All entropic phenomena associated with the protein during crystallization are unfavorable: ¾ loss of degrees of freedom due to incorporation of molecules into the lattice which is an unavoidable entropic cost of crystallization; 30 – 100 kJ mol-1 at room T ¾ loss of entropy due to ordering of otherw ...
... All entropic phenomena associated with the protein during crystallization are unfavorable: ¾ loss of degrees of freedom due to incorporation of molecules into the lattice which is an unavoidable entropic cost of crystallization; 30 – 100 kJ mol-1 at room T ¾ loss of entropy due to ordering of otherw ...
Electrochromatography
... the "radius of gyration" discussed in polymer physics must usually be understood as a mean over all polymer molecules of the sample and over time Rg determined experimentally with static light scattering as well as with small angle neutron- and x-ray scattering. The hydrodynamic radius is nume ...
... the "radius of gyration" discussed in polymer physics must usually be understood as a mean over all polymer molecules of the sample and over time Rg determined experimentally with static light scattering as well as with small angle neutron- and x-ray scattering. The hydrodynamic radius is nume ...
Role of basic character of α-sarcin`s NH2-terminal β
... explain the ability of α-sarcin to interact with phospholipid bilayers, the protein would be initially adsorbed to the charged polar head groups of the phospholipids, and then would partially penetrate the interface of the bilayer to interact with a portion of the lipid hydrocarbon chains [1,6,26,31 ...
... explain the ability of α-sarcin to interact with phospholipid bilayers, the protein would be initially adsorbed to the charged polar head groups of the phospholipids, and then would partially penetrate the interface of the bilayer to interact with a portion of the lipid hydrocarbon chains [1,6,26,31 ...
Using storage organelles for the accumulation and encapsulation of
... available within a species, such as transgenic tobacco, transplastomic tobacco, transient expression in tobacco using Agrobacterium tumefaciens, plant viruses or hybrids thereof, hydroponic tobacco root secretion, hairy root cultures, and tobacco suspension cells [5]. This diversity has resulted in ...
... available within a species, such as transgenic tobacco, transplastomic tobacco, transient expression in tobacco using Agrobacterium tumefaciens, plant viruses or hybrids thereof, hydroponic tobacco root secretion, hairy root cultures, and tobacco suspension cells [5]. This diversity has resulted in ...
molecular biology and phylogeny
... PROCEDURES: You have already done and discussed the activity entitled "Making Cladograms". The final cladogram produced in that activity (using anatomical similarities) is shown below. The provided chart shows the amino acid sequence in a protein that is homologous (same) for the 20 organisms shown, ...
... PROCEDURES: You have already done and discussed the activity entitled "Making Cladograms". The final cladogram produced in that activity (using anatomical similarities) is shown below. The provided chart shows the amino acid sequence in a protein that is homologous (same) for the 20 organisms shown, ...
DNA, RNA, Protein Synthesis
... • This process takes place in the cytoplasm on ribosomes • Both mRNA and tRNA are necessary for this process ...
... • This process takes place in the cytoplasm on ribosomes • Both mRNA and tRNA are necessary for this process ...
Carbohydrates, Lipids, and proteins
... Both of the functional groups are covalently bonded to a central atom, called the alpha carbon Also bonded to the alpha carbon is a hydrogen atom and a chemical group symbolized by the letter R. R group is the variable part of an amino acid. R group structure determines the specific properties o ...
... Both of the functional groups are covalently bonded to a central atom, called the alpha carbon Also bonded to the alpha carbon is a hydrogen atom and a chemical group symbolized by the letter R. R group is the variable part of an amino acid. R group structure determines the specific properties o ...
Advances in Natural and Applied Sciences Aedes aegypti Aulanni’am
... by 12% SDS-PAGE from lab. scale cultures and from landing populations. Identification of immunogenic proteins from both sample was carried out by using Western Blot Analysis after cross reaction of Salivary Gland Extract (SGE) with 3 different human sera: from DHF patients, healthy persons who were ...
... by 12% SDS-PAGE from lab. scale cultures and from landing populations. Identification of immunogenic proteins from both sample was carried out by using Western Blot Analysis after cross reaction of Salivary Gland Extract (SGE) with 3 different human sera: from DHF patients, healthy persons who were ...
Lipids affect the function of membrane proteins
... Surprisingly, they saw this effect even when the lipids weren’t forming a bilayer, indicating that they behave in some ways like allosteric ligands. The scientists don’t know how much these results will predict what’s happening in actual mammalian membranes, Govaerts says, because those membranes hav ...
... Surprisingly, they saw this effect even when the lipids weren’t forming a bilayer, indicating that they behave in some ways like allosteric ligands. The scientists don’t know how much these results will predict what’s happening in actual mammalian membranes, Govaerts says, because those membranes hav ...
PowerPoint Presentation - Nerve activates contraction
... – Chemical energy used by all cells – Energy is released by breaking high energy ...
... – Chemical energy used by all cells – Energy is released by breaking high energy ...
Seminal Proteins
... Single and double stained Band shift PAGE assay: Although band shift PAGEs were originally designed to detect electrophoretic band dislocation in nucleic acids only and not in proteins [12], but the inclusion of double staining technique for the same gel could sometimes considered as an invaluable a ...
... Single and double stained Band shift PAGE assay: Although band shift PAGEs were originally designed to detect electrophoretic band dislocation in nucleic acids only and not in proteins [12], but the inclusion of double staining technique for the same gel could sometimes considered as an invaluable a ...
Organic Chemistry Answer Key
... A. It adjusts the pH of the reaction medium. B. It provides energy to carry out the reaction. C. It dissolves substance A in the reaction medium. D. It speeds up the reaction without being consumed. A scientist observes that, when the pH of the environment surrounding an enzyme is changed, the r ...
... A. It adjusts the pH of the reaction medium. B. It provides energy to carry out the reaction. C. It dissolves substance A in the reaction medium. D. It speeds up the reaction without being consumed. A scientist observes that, when the pH of the environment surrounding an enzyme is changed, the r ...
Nine essential amino acids
... “Most common dietary proteins contain all essential and non-essential amino acids in various concentrations. Some proteins, especially plant origin proteins, have been termed incomplete, because they do not contain the essential amino acids at the recommended level, while others, especially animal s ...
... “Most common dietary proteins contain all essential and non-essential amino acids in various concentrations. Some proteins, especially plant origin proteins, have been termed incomplete, because they do not contain the essential amino acids at the recommended level, while others, especially animal s ...
Macro-molecules short 2014
... The necessary elements can be obtained from any food (carbs, proteins, lipids) Phosphorus must come from our diet as well Choose you favorite Thanksgiving food and start a rumor that it is high in nucleic acids. Then use this to justify eating a lot of it on Thanksgiving ...
... The necessary elements can be obtained from any food (carbs, proteins, lipids) Phosphorus must come from our diet as well Choose you favorite Thanksgiving food and start a rumor that it is high in nucleic acids. Then use this to justify eating a lot of it on Thanksgiving ...
Antigenic Properties of Mycoplasma Organisms and Membranes
... high values for complement-fixing and growth-inhibiting antibodies. In contrast, the serological activity of the lipid fraction of M. mycoides var. mycoides was limited. Complement-fixing activity and precipitin reactions could be attributed to the galactan produced and excreted by this organism (Ma ...
... high values for complement-fixing and growth-inhibiting antibodies. In contrast, the serological activity of the lipid fraction of M. mycoides var. mycoides was limited. Complement-fixing activity and precipitin reactions could be attributed to the galactan produced and excreted by this organism (Ma ...
Document
... Kreb's Cycle and involves the physical addition of a free phosphate to ADP to form ATP. Oxidative phosphorylation, on the other hand, takes place along the electron transport chain, where ATP is synthesized indirectly from the creation of a proton gradient and the movement of these protons back accr ...
... Kreb's Cycle and involves the physical addition of a free phosphate to ADP to form ATP. Oxidative phosphorylation, on the other hand, takes place along the electron transport chain, where ATP is synthesized indirectly from the creation of a proton gradient and the movement of these protons back accr ...
Molecular Reaction Mechanisms of Proteins
... cm–1) (Colthup et al., 1990). The major problem in measuring reactions consists in selecting the small absorption bands of the molecular groups which undergo reactions from the large background absorption of water and of the quiescent entire protein. The absorbance bands are selected by the performa ...
... cm–1) (Colthup et al., 1990). The major problem in measuring reactions consists in selecting the small absorption bands of the molecular groups which undergo reactions from the large background absorption of water and of the quiescent entire protein. The absorbance bands are selected by the performa ...
to find the lecture notes for lecture 5 cellular communication click here
... to cyclic AMP (cAMP) in the cytosol – cAMP acts as a 2nd messenger – cAMP activates a series of proteins in the cytosol called kinases – kinases act to phosphorylate their targets – either activating them or inhibiting them – this speeds up/slows down physiological responses within the cell – phosph ...
... to cyclic AMP (cAMP) in the cytosol – cAMP acts as a 2nd messenger – cAMP activates a series of proteins in the cytosol called kinases – kinases act to phosphorylate their targets – either activating them or inhibiting them – this speeds up/slows down physiological responses within the cell – phosph ...
binding domains demonstrated in a plant split
... cocktail for plant cell extracts (Sigma P9599) was added to the extraction buffer. Proteins were concentrated by chloroform–methanol precipitation (Wessel and Flugge, 1984) and dissolved in SDS–PAGE sample buffer (50 mM TRIS pH 6.8, 10% glycerol, 2% b-mercaptoethanol, 0.025% bromophenol blue, 2% SDS ...
... cocktail for plant cell extracts (Sigma P9599) was added to the extraction buffer. Proteins were concentrated by chloroform–methanol precipitation (Wessel and Flugge, 1984) and dissolved in SDS–PAGE sample buffer (50 mM TRIS pH 6.8, 10% glycerol, 2% b-mercaptoethanol, 0.025% bromophenol blue, 2% SDS ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.