Gene Section AFF3 (lymphoid nuclear protein related to AF4)
... Coding sequence of LAF4 compared to AF4 and site of fusion. Schematic representation of MLL, LAF4, AF4, and the putative MLL-LAF4 fusion protein. Domains in MLL are shaded: MT, DNA methyltransferase homology region; TRX, Drosophila trithorax homology. The percentage of amino acid homology between co ...
... Coding sequence of LAF4 compared to AF4 and site of fusion. Schematic representation of MLL, LAF4, AF4, and the putative MLL-LAF4 fusion protein. Domains in MLL are shaded: MT, DNA methyltransferase homology region; TRX, Drosophila trithorax homology. The percentage of amino acid homology between co ...
The Synthesis of Proteins
... • There are four kinds of bases (A, C, G, T) in DNA and 20 amino acids. If each base coded for one particular amino acid then only 4 of the 20 amino acids could be used in protein synthesis. If a pair of bases coded for an amino acid then 16 different amino acids could be used. This is still not eno ...
... • There are four kinds of bases (A, C, G, T) in DNA and 20 amino acids. If each base coded for one particular amino acid then only 4 of the 20 amino acids could be used in protein synthesis. If a pair of bases coded for an amino acid then 16 different amino acids could be used. This is still not eno ...
protein factory ingredient info
... Dextrose Dextrose is a high glycemic carbohydrate that should be used for pre or post workout supplementation. The objective in using dextrose is to cause a rapid increase in insulin levels thus shuttling amino acids into the muscle cells. This method of combining carbohydrates and protein before an ...
... Dextrose Dextrose is a high glycemic carbohydrate that should be used for pre or post workout supplementation. The objective in using dextrose is to cause a rapid increase in insulin levels thus shuttling amino acids into the muscle cells. This method of combining carbohydrates and protein before an ...
Title goes here
... Select significant alignments and make profile (weight matrix) using techniques for sequence weighting and pseudo counts Use weight matrix to align against sequence database to find new significant hits Repeat 2 and 3 (normally 3 times!) ...
... Select significant alignments and make profile (weight matrix) using techniques for sequence weighting and pseudo counts Use weight matrix to align against sequence database to find new significant hits Repeat 2 and 3 (normally 3 times!) ...
Properties of Amino Acids
... • The substrate concentration that produces a Vi that is one-half of Vmax is designated the Michaelis-Menten constant, Km(named after the scientists who developed the study of enzyme kinetics). • Km is (roughly) an inverse measure of the affinity or strength of binding between the enzyme and its sub ...
... • The substrate concentration that produces a Vi that is one-half of Vmax is designated the Michaelis-Menten constant, Km(named after the scientists who developed the study of enzyme kinetics). • Km is (roughly) an inverse measure of the affinity or strength of binding between the enzyme and its sub ...
Macromolecules Vocabulary and Concepts
... ? How does the degree of saturation of fatty acids in a cell membrane affect the fluidity of the membrane? o Steroids o Waxes o Vitamins – some vitamins are not water soluble (A, D, E, and K) Protein Many Uses: structural support, protection, transport, catalysis (enzymes), defense, regulation (ho ...
... ? How does the degree of saturation of fatty acids in a cell membrane affect the fluidity of the membrane? o Steroids o Waxes o Vitamins – some vitamins are not water soluble (A, D, E, and K) Protein Many Uses: structural support, protection, transport, catalysis (enzymes), defense, regulation (ho ...
Enzyme kineics
... tracing of the a-carbon atoms. N and C correspond to the amino- and carboxyl-terminals, respectively. The letters followed by numbers represent amino acids in the polypeptide chain. A hypothetical vertical line divides the actin molecule into two domains "large", left side, and "small", right side. ...
... tracing of the a-carbon atoms. N and C correspond to the amino- and carboxyl-terminals, respectively. The letters followed by numbers represent amino acids in the polypeptide chain. A hypothetical vertical line divides the actin molecule into two domains "large", left side, and "small", right side. ...
birkbeck college - Principles of Protein Structure
... The amino acid tyrosine can undertake four types of interaction with neighbouring atoms or groups within a protein molecule. Describe these interactions in detail. [10 Marks] ...
... The amino acid tyrosine can undertake four types of interaction with neighbouring atoms or groups within a protein molecule. Describe these interactions in detail. [10 Marks] ...
Assessing Methods of Detecting Osteogenesis Imperfecta.
... The collagen and genetic samples were obtained from skin fibroblasts from a young boy suspected of having OI, as this is far less invasive than obtaining collagen from placental or amniotic cells. Protein analysis of collagen using PAGE is basically a matter of differentiating a control sample of no ...
... The collagen and genetic samples were obtained from skin fibroblasts from a young boy suspected of having OI, as this is far less invasive than obtaining collagen from placental or amniotic cells. Protein analysis of collagen using PAGE is basically a matter of differentiating a control sample of no ...
lecture 7
... - prefoldin can stabilize an unfolded protein for subsequent folding by chaperonin (explanation in class) - range of proteins archaeal prefoldin stabilizes is considerable: 14-62 kDa Archaeal prefoldin (with 2 different subunits) may play a general role in protein folding whereas the eukaryotic ch ...
... - prefoldin can stabilize an unfolded protein for subsequent folding by chaperonin (explanation in class) - range of proteins archaeal prefoldin stabilizes is considerable: 14-62 kDa Archaeal prefoldin (with 2 different subunits) may play a general role in protein folding whereas the eukaryotic ch ...
The trans-Golgi network GRIP-domain proteins form α
... Cell extracts were diluted in 50 mM Tris/HCl (pH 6.8) containing 100 mM dithiothreitol, 2 % (w/v) SDS and 10 % (v/v) glycerol and subjected to SDS/PAGE. Proteins were then transferred on to PVDF membranes, and the membrane was dried. Antibodies were diluted in PBS containing 5 % (w/v) skim milk powd ...
... Cell extracts were diluted in 50 mM Tris/HCl (pH 6.8) containing 100 mM dithiothreitol, 2 % (w/v) SDS and 10 % (v/v) glycerol and subjected to SDS/PAGE. Proteins were then transferred on to PVDF membranes, and the membrane was dried. Antibodies were diluted in PBS containing 5 % (w/v) skim milk powd ...
Study Guide for Midterm 3 – Chem 109 C
... to steric repulsion - adjacent amino acids can't have the same charge ( for example two lys) due to electrostatic repulsion B. Beta pleated sheet - extended zigzag structure - parallel beta sheets have the adjacent chains both running N end to C end - anti parallel beta sheets run in opposite direct ...
... to steric repulsion - adjacent amino acids can't have the same charge ( for example two lys) due to electrostatic repulsion B. Beta pleated sheet - extended zigzag structure - parallel beta sheets have the adjacent chains both running N end to C end - anti parallel beta sheets run in opposite direct ...
Coming Soon !!! The next lecture will review step 4 and cover this as
... 1- Get the amino acid sequence of the protein. (Primary structure) 2- Search for other proteins with high homology and known structure. 3- Model the amino acid sequence of the query protein onto the fold of the homologous template protein, use simulated molecular dynamics to allow the new amino acid ...
... 1- Get the amino acid sequence of the protein. (Primary structure) 2- Search for other proteins with high homology and known structure. 3- Model the amino acid sequence of the query protein onto the fold of the homologous template protein, use simulated molecular dynamics to allow the new amino acid ...
Purification and proteomic characterization of plastids from Brassica
... [21]. We present a modified procedure here for rapeseed embryos. With any protoplast isolation method it is necessary to physically abrade the plant material initially to increase the surface area for cell wall digestion. Several methods were tested including pressing between glass plates and mincin ...
... [21]. We present a modified procedure here for rapeseed embryos. With any protoplast isolation method it is necessary to physically abrade the plant material initially to increase the surface area for cell wall digestion. Several methods were tested including pressing between glass plates and mincin ...
Beef Cookery - Georgia Beef Board
... Important for muscle fiber structure Stromal proteins (connective tissues) Appears in muscles as collagen (white, thin, transparent) and elastin (yellow) Collagen needs moist heat to convert to tender gelatin Remove collagen if using dry heat Elastin should be removed prior to cooking ...
... Important for muscle fiber structure Stromal proteins (connective tissues) Appears in muscles as collagen (white, thin, transparent) and elastin (yellow) Collagen needs moist heat to convert to tender gelatin Remove collagen if using dry heat Elastin should be removed prior to cooking ...
Macromolecules
... Several amino acids linked together are known as a polypeptide. Denaturing of Proteins: Proteins have a specific structure, which is important for their function. If the structure is distorted or destroyed by heat / ionic concentration / pH change, then the protein is said to be ‘denatured’. Denatur ...
... Several amino acids linked together are known as a polypeptide. Denaturing of Proteins: Proteins have a specific structure, which is important for their function. If the structure is distorted or destroyed by heat / ionic concentration / pH change, then the protein is said to be ‘denatured’. Denatur ...
to get the file - Chair of Computational Biology
... What is “Membrane Bioinformatics” ? Increasing interest in structure & function of membrane proteins (ion channels, G-protein coupled receptors), but only few structures are known structure prediction of membrane proteins, prediction of function from ...
... What is “Membrane Bioinformatics” ? Increasing interest in structure & function of membrane proteins (ion channels, G-protein coupled receptors), but only few structures are known structure prediction of membrane proteins, prediction of function from ...
Hydrophobic Interaction Chromatography (HIC
... Hydrophobic* Interaction Chromatography (HIC) protocol for the protein purification station *Hydrophobic literally means “water fearing.” It refers to substances that do not mix well with water (i.e., Oil is hydrophobic). Note how it stays together in small beads and does not mix with water. Purpose ...
... Hydrophobic* Interaction Chromatography (HIC) protocol for the protein purification station *Hydrophobic literally means “water fearing.” It refers to substances that do not mix well with water (i.e., Oil is hydrophobic). Note how it stays together in small beads and does not mix with water. Purpose ...
Domain fusion between SNF1-related kinase subunits during plant
... whether AKINβγ proteins establish similar associations with plant kinases, we assayed for interaction of ZmAKINβγ-1 with Arabidopsis SNF1-related kinase AKIN11 (Bhalerao et al., 1999). Full-length ZmAKINβγ-1 and two derivatives lacking the SNF4like domain (ZmAKINβγ∆γ-1) or the KIS motif (ZmAKINβγ∆K- ...
... whether AKINβγ proteins establish similar associations with plant kinases, we assayed for interaction of ZmAKINβγ-1 with Arabidopsis SNF1-related kinase AKIN11 (Bhalerao et al., 1999). Full-length ZmAKINβγ-1 and two derivatives lacking the SNF4like domain (ZmAKINβγ∆γ-1) or the KIS motif (ZmAKINβγ∆K- ...
What are Membranes?
... exchanger and glycophorin. The proteins span the membrane and are tethered to spectrin, a cytoskeletal protein, by another protein, ankyrin, limiting their lateral mobility. Ankyrin is anchored in the membrane by a covalently bound palmitoyl side chain (see Figure 11-14). Spectrin, a long, filamento ...
... exchanger and glycophorin. The proteins span the membrane and are tethered to spectrin, a cytoskeletal protein, by another protein, ankyrin, limiting their lateral mobility. Ankyrin is anchored in the membrane by a covalently bound palmitoyl side chain (see Figure 11-14). Spectrin, a long, filamento ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.