Amino acid sequence restriction in relation to proteolysis
... s e n s i t i v e s i t e s in regions not s t a b i l i z e d by secondary structure. C o n s e q u e n t l y , dibasic structures not stabilized by either secondary structure or subsequent proline appear to form a type of 'restricted sequence' in proteins not destined for proteolysis. Thus, both s ...
... s e n s i t i v e s i t e s in regions not s t a b i l i z e d by secondary structure. C o n s e q u e n t l y , dibasic structures not stabilized by either secondary structure or subsequent proline appear to form a type of 'restricted sequence' in proteins not destined for proteolysis. Thus, both s ...
Proteomics Analysis of Immunoprecipitated Proteins Associated with
... be largely responsible for the difference in activity. A putative PEST sequence was also found between residues 415 and 438. In this study, we attempted to determine potential binding partners to Cot and their region of association by separating proteins that were pulled-down with various Cot constr ...
... be largely responsible for the difference in activity. A putative PEST sequence was also found between residues 415 and 438. In this study, we attempted to determine potential binding partners to Cot and their region of association by separating proteins that were pulled-down with various Cot constr ...
nerve agents - 20 Years of OPCW
... of the protein leading to its three dimensional structure. Helical substructures and so called beta-sheets (thick arrows) can be identified. The catalytic active site is buried deep inside the enzyme. It contains three amino acids crucial for catalytic activity: Serine 200, Histidine 440 and Glutama ...
... of the protein leading to its three dimensional structure. Helical substructures and so called beta-sheets (thick arrows) can be identified. The catalytic active site is buried deep inside the enzyme. It contains three amino acids crucial for catalytic activity: Serine 200, Histidine 440 and Glutama ...
Steroid and Thyroid Hormones
... e. It occurs in 2 ways. You can have double iodination, giving you 2 di-iodotyrosine, or you can have monoiodination giving you mono-iodotyrosine. f. Then there is an enzyme called a coupling enzyme that will piggyback one of these tyrosines on top of the other. It will transfer a portion of the iod ...
... e. It occurs in 2 ways. You can have double iodination, giving you 2 di-iodotyrosine, or you can have monoiodination giving you mono-iodotyrosine. f. Then there is an enzyme called a coupling enzyme that will piggyback one of these tyrosines on top of the other. It will transfer a portion of the iod ...
proteinCompression
... PROJECT DNA and Protein form the basic structure of the life and they can be represented by any normal text file. When it comes to compression, we see that they don’t show the good result from the normal compression techniques. In this project we have used a new technique called CP (Compressio ...
... PROJECT DNA and Protein form the basic structure of the life and they can be represented by any normal text file. When it comes to compression, we see that they don’t show the good result from the normal compression techniques. In this project we have used a new technique called CP (Compressio ...
Morphologically distinct phenotypes of spermatozoa in infertile men
... be identified by routine semen analysis because they are but secondary manifestations of underlying pathology. We hypothesize that proteomics would enable us to better understand the mechanism(s) underlying these pathologies. DESIGN: This proteomic study analyzed proteins in spermatozoa from inferti ...
... be identified by routine semen analysis because they are but secondary manifestations of underlying pathology. We hypothesize that proteomics would enable us to better understand the mechanism(s) underlying these pathologies. DESIGN: This proteomic study analyzed proteins in spermatozoa from inferti ...
The Escherichia coli SlyD Is a Metal Ion-regulated Peptidyl
... Hippel (23). Polyacrylamide gel electrophoresis was performed as described by Laemmli (19), and the protein bands were visualized by silver staining (24) or a modified Coomassie Blue staining method (20). Polyclonal antibodies against SlyD and SlpA were raised in rabbits (PAB productions, Hebertshau ...
... Hippel (23). Polyacrylamide gel electrophoresis was performed as described by Laemmli (19), and the protein bands were visualized by silver staining (24) or a modified Coomassie Blue staining method (20). Polyclonal antibodies against SlyD and SlpA were raised in rabbits (PAB productions, Hebertshau ...
Human stomach tissue lysate - soluble fraction (male, 66
... to ab44463: Extraction 1: soluble protein fraction ab44464 Human Stomach Tumor Tissue lysate ab44463 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44466 Human Stomach Tumor Tissue lysate ab44465 Human Stomach Normal Tissue lysate (matched) ...
... to ab44463: Extraction 1: soluble protein fraction ab44464 Human Stomach Tumor Tissue lysate ab44463 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44466 Human Stomach Tumor Tissue lysate ab44465 Human Stomach Normal Tissue lysate (matched) ...
File
... Diatoms are unicellular algae which live as plankton in fresh water and oceans. Biochemists investigated two species of diatom, Thalassiosira oceanica which lives in the open ocean where the water is clear and Thalassiosira weissflogii which lives in coastal waters where the water is often cloudy. I ...
... Diatoms are unicellular algae which live as plankton in fresh water and oceans. Biochemists investigated two species of diatom, Thalassiosira oceanica which lives in the open ocean where the water is clear and Thalassiosira weissflogii which lives in coastal waters where the water is often cloudy. I ...
File
... Cut out the mRNA strand and stick it together. Now cut out the 9 amino acid templates, then cut out the 9 R-groups to make 9 specific amino acids. ...
... Cut out the mRNA strand and stick it together. Now cut out the 9 amino acid templates, then cut out the 9 R-groups to make 9 specific amino acids. ...
Rampant Purifying Selection Conserves Positions with
... Posttranslational modifications (PTMs) fine-tune biochemical functions in a large percentage of human proteins (Mann and Jensen 2003; Seo and Lee 2004). In particular, phosphorylation and glycosylation of amino acid positions in thousands of human proteins are now known, many of which have been impl ...
... Posttranslational modifications (PTMs) fine-tune biochemical functions in a large percentage of human proteins (Mann and Jensen 2003; Seo and Lee 2004). In particular, phosphorylation and glycosylation of amino acid positions in thousands of human proteins are now known, many of which have been impl ...
Role of Interactions and Volume Variation in Discriminating Active
... complexes. Analysis and visualization of interactions in a set of protein-ligand complexes provide insight for structure-based drug design. In the present study, we have made a comprehensive analysis on similarities and differences observed in hydrophobic interactions and hydrogen bond interactions ...
... complexes. Analysis and visualization of interactions in a set of protein-ligand complexes provide insight for structure-based drug design. In the present study, we have made a comprehensive analysis on similarities and differences observed in hydrophobic interactions and hydrogen bond interactions ...
HMG B domain
... deletion of 11 residues between the the first and second AT hook in the latter. Alternative splicing also produces HMGA1c. The related HMGA2 (HMGI-C) protein is coded for by a separate gene. ...
... deletion of 11 residues between the the first and second AT hook in the latter. Alternative splicing also produces HMGA1c. The related HMGA2 (HMGI-C) protein is coded for by a separate gene. ...
Three-Dimensional Structure of Adenosylcobinamide Kinase
... the site for guanylylation is most likely a histidine (10). Since CobU requires GTP for the transferase activity and can utilize either ATP or GTP for the kinase activity, presumably there must be two separate binding sites for these nucleotides and hence two active sites. Thus CobU presents several ...
... the site for guanylylation is most likely a histidine (10). Since CobU requires GTP for the transferase activity and can utilize either ATP or GTP for the kinase activity, presumably there must be two separate binding sites for these nucleotides and hence two active sites. Thus CobU presents several ...
Fast Separation of Recombinant Human Erythropoietin
... of rEPO protein will be altered significantly. The lower the pH, the more changes1. Figure 3 shows both conditions of heating. Panel A shows data from a sample was heated at neutral pH. The column resolved the main peak of rEPO from its degraded products or isoforms very well. Note that the conforma ...
... of rEPO protein will be altered significantly. The lower the pH, the more changes1. Figure 3 shows both conditions of heating. Panel A shows data from a sample was heated at neutral pH. The column resolved the main peak of rEPO from its degraded products or isoforms very well. Note that the conforma ...
Transmembrane domains control exclusion of membrane proteins
... to the CD1b heavy chain ensured that assembly of CD1b was independent of endogenous b2-microglobulin (Im et al., 2004). In mammalian cells, TMDs were shown previously to be responsible for targeting of membrane proteins to the endoplasmic reticulum (Bonifacino et al., 1991) or to the Golgi apparatus ...
... to the CD1b heavy chain ensured that assembly of CD1b was independent of endogenous b2-microglobulin (Im et al., 2004). In mammalian cells, TMDs were shown previously to be responsible for targeting of membrane proteins to the endoplasmic reticulum (Bonifacino et al., 1991) or to the Golgi apparatus ...
Sauer, N. and Tanner, W.
... 2.3. Southern and Northern blot analysis 2.3.1. Southern blot DNA was isolated [12], digested with several restriction enzymes (see fig.3), separated on a 0.8% agarose gel, and blotted onto a nitrocellulose filter. The filter was probed with the cDNA clone pTF14, which starts at base 1042 of the seq ...
... 2.3. Southern and Northern blot analysis 2.3.1. Southern blot DNA was isolated [12], digested with several restriction enzymes (see fig.3), separated on a 0.8% agarose gel, and blotted onto a nitrocellulose filter. The filter was probed with the cDNA clone pTF14, which starts at base 1042 of the seq ...
Applied and Environmental Microbiology
... cell surface-exposed epitopes to mark P. putida, a phoE-caa hybrid gene was constructed. For this purpose, plasmid pMR05, which carries aphoE gene with an NruI restriction site in the sequence coding for Asn-157 to Asp-159, which forms part of the fourth cell surface-exposed loop of the PhoE protein ...
... cell surface-exposed epitopes to mark P. putida, a phoE-caa hybrid gene was constructed. For this purpose, plasmid pMR05, which carries aphoE gene with an NruI restriction site in the sequence coding for Asn-157 to Asp-159, which forms part of the fourth cell surface-exposed loop of the PhoE protein ...
The effect of pH on the digestion of proteins in vitro by pepsin
... peptides from any of the digests of the soya-protein samples by either electrophoretic or chromatographic methods. DISCUSSION ...
... peptides from any of the digests of the soya-protein samples by either electrophoretic or chromatographic methods. DISCUSSION ...
Computational method on biochemistry
... • However, AMBER has been widely used not only for proteins and DNA, but also for many other classes of models, such as polymers and small molecules. For the latter classes of models, various authors have added parameters and extended AMBER in other ways to suit their calculations. The AMBER forcefi ...
... • However, AMBER has been widely used not only for proteins and DNA, but also for many other classes of models, such as polymers and small molecules. For the latter classes of models, various authors have added parameters and extended AMBER in other ways to suit their calculations. The AMBER forcefi ...
Proteome of amyloplasts isolated from developing wheat
... thawed three times to break the intact organelles. The lysate was centrifuged (10 000 g, 20 min, 4 8C) to collect the soluble proteins (supernatant fraction). The pellet, containing starch granules and other insoluble materials, was extracted with the same buffer plus 2% Triton X-100 and centrifuged ...
... thawed three times to break the intact organelles. The lysate was centrifuged (10 000 g, 20 min, 4 8C) to collect the soluble proteins (supernatant fraction). The pellet, containing starch granules and other insoluble materials, was extracted with the same buffer plus 2% Triton X-100 and centrifuged ...
Chapter 17 From Gene to Protein
... Translation- cellular process that converts the mRNA codons into amino acids to build proteins. First let’s practice reading the mRNA into amino acids and then I will outline the process of how it’s done step by step. Look at the sequence of mRNA below and the chart in Fig. ___ on page _____. ...
... Translation- cellular process that converts the mRNA codons into amino acids to build proteins. First let’s practice reading the mRNA into amino acids and then I will outline the process of how it’s done step by step. Look at the sequence of mRNA below and the chart in Fig. ___ on page _____. ...
Biochemistry of cell organelles
... • Overall structure of mitochondria, their shape and size; • Origin of mitochondria; • Dynamic behaviour of mitochondria; • Composition of mitochondria, functional role of different subcompartments; • Isolation of mitochondria, how to analyse the composition of mitochondria; • Visualization (imaging ...
... • Overall structure of mitochondria, their shape and size; • Origin of mitochondria; • Dynamic behaviour of mitochondria; • Composition of mitochondria, functional role of different subcompartments; • Isolation of mitochondria, how to analyse the composition of mitochondria; • Visualization (imaging ...
Endocytosis-like protein uptake in the bacterium Gemmata
... immunogold labeling of high-pressure frozen cells to investigate whether the internalized GFP was associated with any specific intracellular structures. Gold particles indicating presence of GFP in the paryphoplasm were often seen lining vesicle-like entities ca. 50–200 nm wide bounded by a single bi ...
... immunogold labeling of high-pressure frozen cells to investigate whether the internalized GFP was associated with any specific intracellular structures. Gold particles indicating presence of GFP in the paryphoplasm were often seen lining vesicle-like entities ca. 50–200 nm wide bounded by a single bi ...
08. mechanism of uptake - physiological role of nutrients
... According to this theory there are certain pre existing ions inside the cell which cannot diffuse outside through membrane. Such ions are called as in diffusible or fixed ions. However, the membrane is permeable to both anions and cations of the outer solutions. Suppose there are certain fixed anion ...
... According to this theory there are certain pre existing ions inside the cell which cannot diffuse outside through membrane. Such ions are called as in diffusible or fixed ions. However, the membrane is permeable to both anions and cations of the outer solutions. Suppose there are certain fixed anion ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.