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GR Safe I Nucleic Acid Gel Stain, 10,000X in Water
Catalog #: IV-1001
http://www.labsupplymall.com
Store at 4 oC or Room Temperature
without UV light using blue light
Introduction
transilluminator.
GR Safe I is a new, safe nucleic acid stain for
detecting double-stranded DNA, single-stranded DNA,
and RNA in agarose gel. It can be used for replacing
mutagenic ethidium bromide (EB). GR Safe emits
green fluorescence when bound to dsDNA and red
fluorescence when bound to ssDNA or RNA. This new
stain has two fluorescence excitation maxima when
bound to nucleic acid, one centered at approximately
290 nm and one at approximately 490nm. GR Safe is
as sensitive as EB, and much cheaper than SYBR
Green, SYBR Gold and SYBR Safe, and you can use
GR Safe just as the way you used EB.

Cut out DNA bands for subclonning under safer
blue light: No mutations caused by EB and UV
light.
Compared to EB which is a very strong mutagen, GR
Safe caused very few mutations in the Ames test. In
addition, GR Safe had a negative result in mouse
marrow chromophilous erythrocyte micronucleus test
and mouse spermary spermatocyte chromosomal
aberration test.


Available at 10,000X in H2O for better safety
No more toxic and flammable organic solvent
Room temperature storage for better
convenience, stable at room temperature for
years --No more freeze-and-thaw cycle!
Fig. 1, Comparison of GR Safe II with EtBr.
Top: GR Safe I. Bottom: EtBr.
Storage: Store at room temperature.

Sensitivity is comparable to Ethidium Bromide
and SYBR Safe.
Disposal:

Add GR Safe to warm agarose gel solution as
you did with ethidium bromide before--No
need to do lengthy post electrophoresis staining,
save your valuable time..
Gel: Biosafety trash bag.
TAE, TBE or Sodium Borate Buffers: sink or
consult a chemical safety officer at your
institution.

Compatible with UV
transilluminator
and
documentation systems.
Protocol:

Will not affect downstream experiments:
compatible with all gel purification kits tested,
will not inhibit ligation reaction etc.

Compatible
with
Sodium
Borate
Electrophoresis Buffer: Run gel 2-3 times faster
at higher voltage, resolve shaper bands in
minutes, and less heat generation.

or blue
common
light
gel
Watch DNA migrate at your bench, in real-time
1. Prepare 40 ml of agarose gel solution
(concentration from 0.8~2.0%) with TAE or
Borate Buffer in a 250 ml flask and mix it
thoroughly. Place the flask in the microware,
heat on high until the solution is completely
clear and no small floating particles are visible
(about 2~3 minutes).
2. After the gel solution cool to about 55 oC,
add 1 to 4 µl of GR Safe to the solution. Swirl
©Copyright Lab Supply Mall, InnoVita Inc
847 Quince Orchard Blvd, Suite H, Gaithersburg, MD, 20878, USA  Phone: 888-666-0968  Fax: 301-208-0536
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GR Safe I Nucleic Acid Gel Stain, 10,000X in Water
Catalog #: IV-1001
http://www.labsupplymall.com
Store at 4 oC or Room Temperature
the flask gently to mix the solution and avoid
This item can be purchased from VWR (Cat#
forming bubbles.
15000-088, $318.00).
3. Pour the gel solution into a gel tray until the
comb teeth are immersed about 1/4~1/2 into the
gel solution.
Visi-Blue Converter Plate. 21Wx26Lcm.
Designed to convert UV light to 480nm blue
light for use with GR Safe, SYBR Green,
SYPRO Orange and EGFP stains. Scratch
resistant blue Plexiglas surface is safety fused
into metal frame for durability. For UVP
transilluminators.
4. After the agarose gel has solidified you can
perform electrophoresis using either 1X TAE or
1X Borate Buffer (Available from
http://www.labsupplymall.com).
6, Is is safer than SYBR Safe?
5. Detect the bands using UV or blue light
transilluminator.
It is safer than SYBR Safe.
FAQ
7, Is it more sensitive than SYBR Safe?
1, What filter should I use for blue light
transilluminator?
Similar.
Amber filter. You can buy it from
www.bhphotovideo.com, www.adorama.com,
www.ritzcamera.com or www.wolfcamera.com
2, Where to buy a blue light
transilluminator?
Labsupplymall.com
3, Where to buy blue light LED (torch) for
monitoring gel and cutting DNA band from
gel?
Ebay, Ebay Motors or other on-line stores
4, I got high background, what should I do?
Use less GR Safe, e.g., 1 µl/per 100 ml gel
solution
5, Can I use UV transilluminator?
Yes. You can also convert UV transilluminator
to Blue light transilluminator using a UVP
VISIBLUE CONVRTR PLATE, 21X26 cm.
©Copyright Lab Supply Mall, InnoVita Inc
847 Quince Orchard Blvd, Suite H, Gaithersburg, MD, 20878, USA  Phone: 888-666-0968  Fax: 301-208-0536
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