Expressway Plus - Thermo Fisher Scientific
... High-performance protein expression Traditional protein expression systems involve expressing recombinant proteins in intact eukaryotic or prokaryotic cells. Although effective, these methods can be both time-consuming and technically challenging. The Expressway™ Plus Expression System eliminates t ...
... High-performance protein expression Traditional protein expression systems involve expressing recombinant proteins in intact eukaryotic or prokaryotic cells. Although effective, these methods can be both time-consuming and technically challenging. The Expressway™ Plus Expression System eliminates t ...
蛋白质结构基础(Introduction of Protein Structure)
... The majority of a-helices in globular proteins are curved or distorted somewhat compared with the standard Pauling-Corey model. Why? 1. The packing of buried helices against other secondary structure elements in the core of the protein 2. Proline residues induce distortions of around 20 degrees in t ...
... The majority of a-helices in globular proteins are curved or distorted somewhat compared with the standard Pauling-Corey model. Why? 1. The packing of buried helices against other secondary structure elements in the core of the protein 2. Proline residues induce distortions of around 20 degrees in t ...
Are You Getting It??
... The three base- pairs formed have equal strength. They bind in a antiparallel orientation. The first base in a codon can wobble. The first base in an anticodon can be a rare base. An anticodon can bind to only one codon. ...
... The three base- pairs formed have equal strength. They bind in a antiparallel orientation. The first base in a codon can wobble. The first base in an anticodon can be a rare base. An anticodon can bind to only one codon. ...
the peroxisomal endomembrane system and the role of the ER
... model yeast organism, S. cerevisiae, the entire ER acts as tER, facilitating the budding of COPII-coated vesicles (Rossanese et al., 1999). In contrast, the tER of mammalian cells is organized into discrete ER export sites (Hammond and Glick, 2000). It is therefore possible that by segregating a dis ...
... model yeast organism, S. cerevisiae, the entire ER acts as tER, facilitating the budding of COPII-coated vesicles (Rossanese et al., 1999). In contrast, the tER of mammalian cells is organized into discrete ER export sites (Hammond and Glick, 2000). It is therefore possible that by segregating a dis ...
Key - UCSB CLAS
... secondary ⇒ regular conformations assumed by segments of the protein’s backbone when it folds (in order to maximize H-bonds in the backbone) tertiary ⇒ the 3D structure of the entire protein quaternary ⇒ if a protein has more than one polypeptide chain (aka subunit) the quaternary structure is the w ...
... secondary ⇒ regular conformations assumed by segments of the protein’s backbone when it folds (in order to maximize H-bonds in the backbone) tertiary ⇒ the 3D structure of the entire protein quaternary ⇒ if a protein has more than one polypeptide chain (aka subunit) the quaternary structure is the w ...
Infant formula legislation
... should only be used on advice of a independent health worker as to the need for its use and the proper method of use. The label shall have no pictures of infants and women nor any other picture or text which idealizes the use of infant formula. The terms "humanized", "maternalized" or other simila ...
... should only be used on advice of a independent health worker as to the need for its use and the proper method of use. The label shall have no pictures of infants and women nor any other picture or text which idealizes the use of infant formula. The terms "humanized", "maternalized" or other simila ...
... Bonus (2 pts). Where could you find glycine residues on the Ramachandran plot? Why? The unfavorable regions are due to repulsive van der Waals interactions with the beta carbon. Since glycine has no beta carbon it could be found almost anywhere on the plot. Note: glycine has phi and psi angles and c ...
Identification of the Missing Links in Prokaryotic Pentose Oxidation
... Enzyme reactions were performed with cell-free extract (CFE) from S. solfataricus cultures grown on either D-Ara or D-Glu, which were harvested at mid-exponential phase. The reaction was started by adding 25 l of 3.5 mg/ml CFE to a mixture containing 10 mM potassium D-arabinonate, 1 mM MgCl2, and e ...
... Enzyme reactions were performed with cell-free extract (CFE) from S. solfataricus cultures grown on either D-Ara or D-Glu, which were harvested at mid-exponential phase. The reaction was started by adding 25 l of 3.5 mg/ml CFE to a mixture containing 10 mM potassium D-arabinonate, 1 mM MgCl2, and e ...
Ab initio modelling tutorial (part II)
... • For each phase, Rg, V and its scattering curve can be given • For each curve, contrast of each phase are specified ...
... • For each phase, Rg, V and its scattering curve can be given • For each curve, contrast of each phase are specified ...
The Additive Screen MD1-11
... There are 24 unique reagents supplied in the kit. The concentrations of the reagents have been designed to allow minimum dilution of the sample drop, and are sufficiently concentrated that vapour diffusion experiments can be carried out in as little as a 2.5µl drop. All additives have to be added to ...
... There are 24 unique reagents supplied in the kit. The concentrations of the reagents have been designed to allow minimum dilution of the sample drop, and are sufficiently concentrated that vapour diffusion experiments can be carried out in as little as a 2.5µl drop. All additives have to be added to ...
Playing Biology’s Name Game: Identifying Protein Names In
... An accompanying automatic curation procedure based on a simple token model of protein names An efficient search algorithm to analyze all abstracts in MEDLINE Parameters are optimized using machine learning techniques ...
... An accompanying automatic curation procedure based on a simple token model of protein names An efficient search algorithm to analyze all abstracts in MEDLINE Parameters are optimized using machine learning techniques ...
Practice Exam 1
... I post practice exams to allow you to get a real sense of the experience of taking a Biology 200 exam. The best way to use each exam is as follows. 1. Do NOT answer the questions as a problem set. 2. Study material using your lecture and lab notes and do problem sets FIRST. 3. When you feel you are ...
... I post practice exams to allow you to get a real sense of the experience of taking a Biology 200 exam. The best way to use each exam is as follows. 1. Do NOT answer the questions as a problem set. 2. Study material using your lecture and lab notes and do problem sets FIRST. 3. When you feel you are ...
The subunit of voltage sensitive Ca 2+ channels is a single
... Transmitter release was determined essentially as previously described [26]. Briefly, cells were incubated for 1.5 h at 37°C with 0.5 ml growth medium 0.85 ,ul [3H]DA (41 Ci/mmol) and 10/ag/ml pargyline, followed by extensive washings with medium (3 x 0.5 ml) and release buffer consisting of (mM): 1 ...
... Transmitter release was determined essentially as previously described [26]. Briefly, cells were incubated for 1.5 h at 37°C with 0.5 ml growth medium 0.85 ,ul [3H]DA (41 Ci/mmol) and 10/ag/ml pargyline, followed by extensive washings with medium (3 x 0.5 ml) and release buffer consisting of (mM): 1 ...
No Slide Title
... Amino acids bind, to form a protein. Upon binding, two protons from the NH3 and one oxygen from the carboxyl join to form a water. So the peptide bond has at the one side a C=O and at the other side an N-H. Only the ends of the chain are NH3 or carboxylic, and thus charged. ...
... Amino acids bind, to form a protein. Upon binding, two protons from the NH3 and one oxygen from the carboxyl join to form a water. So the peptide bond has at the one side a C=O and at the other side an N-H. Only the ends of the chain are NH3 or carboxylic, and thus charged. ...
Restriction enzyme
... DNA fragments will stick to a nylon or nitrocellulose membrane. The membrane is laid on top of the agarose gel and absorbent material (e.g. paper towels or a sponge) is placed on top. With time, the DNA fragments will travel from the gel to the membrane by capillary action as surrounding liquid is d ...
... DNA fragments will stick to a nylon or nitrocellulose membrane. The membrane is laid on top of the agarose gel and absorbent material (e.g. paper towels or a sponge) is placed on top. With time, the DNA fragments will travel from the gel to the membrane by capillary action as surrounding liquid is d ...
Q1. Babies find it difficult to digest proteins in their food. Baby food
... He tries four new enzymes, W, X, Y and Z, to see if he can reduce the time taken to predigest the protein. The graph shows the time taken for the enzymes to completely pre-digest the protein. The manufacturer uses the same concentration of enzyme and the same mass of protein in each experiment. ...
... He tries four new enzymes, W, X, Y and Z, to see if he can reduce the time taken to predigest the protein. The graph shows the time taken for the enzymes to completely pre-digest the protein. The manufacturer uses the same concentration of enzyme and the same mass of protein in each experiment. ...
Molecular Affinity and Permeability of Different Molecular Weight
... Molecular Affinity. Chitosan membrane showed a strong adsorption to the bovine serum protein at pH 7 (2.5% v/v). The main adsorption took place in the first hour. The membrane adsorptive capacity reached 3.69, 1.87, and 1.70 mg/g for the 480000, 250000, and 120000 Da chitosan membranes, respectively ...
... Molecular Affinity. Chitosan membrane showed a strong adsorption to the bovine serum protein at pH 7 (2.5% v/v). The main adsorption took place in the first hour. The membrane adsorptive capacity reached 3.69, 1.87, and 1.70 mg/g for the 480000, 250000, and 120000 Da chitosan membranes, respectively ...
Tutorial: Sequence-Based Analysis
... Each row contains a score for the alignment with each possible residue. Profile Searching Summation of Scores for Each Amino Acid Residue along Query Sequence Higher Match Values at Conserved Positions ...
... Each row contains a score for the alignment with each possible residue. Profile Searching Summation of Scores for Each Amino Acid Residue along Query Sequence Higher Match Values at Conserved Positions ...
CONCISE REPORT Late Expression of M and N
... membrane lysates were made with monoclonal antibodies 8 I 59 or 8 161, followed by rabbit anti-mouse Ig using the protein A containing S ...
... membrane lysates were made with monoclonal antibodies 8 I 59 or 8 161, followed by rabbit anti-mouse Ig using the protein A containing S ...
GFP-labelled Rubisco and aspartate aminotransferase are present
... Stable transgenic lines that showed both high and low levels of ¯uorescence were regenerated for each construct. None of the plants showed any obvious changes in growth or morphology relative to the wild type when grown on soil or in culture. In all transgenic lines, ¯uorescence was detected speci®c ...
... Stable transgenic lines that showed both high and low levels of ¯uorescence were regenerated for each construct. None of the plants showed any obvious changes in growth or morphology relative to the wild type when grown on soil or in culture. In all transgenic lines, ¯uorescence was detected speci®c ...
et al - The EMBO Journal
... the presence of the cdka;1 mutant allele (Chen et al, 2009). In depth analysis of the rbr-2 mutant plants revealed that wild-type RBR mRNA and protein are present in vegetative cells. In contrast, in meiocytes only a C-terminal truncated RBR protein is present, due to aberrant RBR mRNA splicing in t ...
... the presence of the cdka;1 mutant allele (Chen et al, 2009). In depth analysis of the rbr-2 mutant plants revealed that wild-type RBR mRNA and protein are present in vegetative cells. In contrast, in meiocytes only a C-terminal truncated RBR protein is present, due to aberrant RBR mRNA splicing in t ...
TIM BARREL
... Luciferase and NFP NFP deletes a 90-residue section that contains an αβαβα segment of TIM barrel Missing segment is compensated for by a single anti-parallel strand Shared regions are 30% identical, they are part of same operon, and homology was detected before Luciferase structure was solved Other ...
... Luciferase and NFP NFP deletes a 90-residue section that contains an αβαβα segment of TIM barrel Missing segment is compensated for by a single anti-parallel strand Shared regions are 30% identical, they are part of same operon, and homology was detected before Luciferase structure was solved Other ...
Reactivities of HIV-1 gag-Derived Peptides with Antibodies of HIV
... infected patients during antiviral treatment. Tests using viral or recombinant p24 antigen on Western blots are, however, hampered by a significant degree of antibody-cross-reactions present in sera of not-infected human individuals. An enzyme-linked immunosorbent assay (ELISA) based on synthetic pe ...
... infected patients during antiviral treatment. Tests using viral or recombinant p24 antigen on Western blots are, however, hampered by a significant degree of antibody-cross-reactions present in sera of not-infected human individuals. An enzyme-linked immunosorbent assay (ELISA) based on synthetic pe ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.