Insect Biochemistry 15:
... Protein was determined according to Bradford (1976), using Coomassie brilliant blue G250. Bovine serum albumin was used as standard. Glutathione, MnCI., and ADP did not give significant colour with this reagent at the levels used. Molecular weight determination The molecular weight of GS was estimat ...
... Protein was determined according to Bradford (1976), using Coomassie brilliant blue G250. Bovine serum albumin was used as standard. Glutathione, MnCI., and ADP did not give significant colour with this reagent at the levels used. Molecular weight determination The molecular weight of GS was estimat ...
Increased Functional Half-life of Fibroblast Growth Factor
... the addition of a novel cysteine that effectively recovers a vestigial disulfide bond of FGF-1. Furthermore, X-ray structure of the oxidized form of Ala66 → Cys mutant shows that the disulfide bond is formed with minimal structural perturbation and in a solvent excluded region of the protein. Theref ...
... the addition of a novel cysteine that effectively recovers a vestigial disulfide bond of FGF-1. Furthermore, X-ray structure of the oxidized form of Ala66 → Cys mutant shows that the disulfide bond is formed with minimal structural perturbation and in a solvent excluded region of the protein. Theref ...
Cis-elements of protein transport to the plant vacuoles
... affinities not detected in this assay system, but at least the aleurain peptide was found to have a much higher affinity for BP-80 than the sporamin peptide ( Kirsch et al., 1994). In a similar work with extracts of vesicles from pumpkin, binding of a 72 and an 82 kDa protein (PV72 and PV82) was obs ...
... affinities not detected in this assay system, but at least the aleurain peptide was found to have a much higher affinity for BP-80 than the sporamin peptide ( Kirsch et al., 1994). In a similar work with extracts of vesicles from pumpkin, binding of a 72 and an 82 kDa protein (PV72 and PV82) was obs ...
slides - NMRbox
... Knowledge of NMR assignments and structure allows careful peak-by-peak analysis which can correlate spectral changes with specific and subtle structural details such as a sidechain reorientation. NMR assignment is complicated, and generally requires 13C / 15N labeled protein. Y Aubin, DJ Hodgson, WB ...
... Knowledge of NMR assignments and structure allows careful peak-by-peak analysis which can correlate spectral changes with specific and subtle structural details such as a sidechain reorientation. NMR assignment is complicated, and generally requires 13C / 15N labeled protein. Y Aubin, DJ Hodgson, WB ...
Macromolecules
... two hydrogen bonds. Base pairs of this size fit perfectly into a double helix. This is the socalled Watson-Crick base pairing pattern. Double helices rich in GC pairs are more stable than those rich in AT (or AU) pairs because GC pairs have more hydrogen bonds Now, Specific AT (or AU) and GC base pa ...
... two hydrogen bonds. Base pairs of this size fit perfectly into a double helix. This is the socalled Watson-Crick base pairing pattern. Double helices rich in GC pairs are more stable than those rich in AT (or AU) pairs because GC pairs have more hydrogen bonds Now, Specific AT (or AU) and GC base pa ...
BIOMOLECULES: INTRODUCTION, STRUCTURE AND FUNCTION
... In the middle of the nineteenth century, the time when new breakthroughs in chemistry and physics were being made virtually everyday, a Dutch scientist Gerardus Mulder described the presence of a substance in living tissues as something which was “without doubt the most important of all substances ...
... In the middle of the nineteenth century, the time when new breakthroughs in chemistry and physics were being made virtually everyday, a Dutch scientist Gerardus Mulder described the presence of a substance in living tissues as something which was “without doubt the most important of all substances ...
Lecture 5 Tues 4-11-06
... a. Differ from Mt because they are surrounded by only a single membrane, do not contain DNA or ribosomes, & acquire all their proteins by selective import from the cytosol b. Post-translational mechanism of protein import like that of the nucleus Does not involve unfolding of the cargo Involves a so ...
... a. Differ from Mt because they are surrounded by only a single membrane, do not contain DNA or ribosomes, & acquire all their proteins by selective import from the cytosol b. Post-translational mechanism of protein import like that of the nucleus Does not involve unfolding of the cargo Involves a so ...
מצגת של PowerPoint
... In this presentation we are dealing only with questions from the three modules which refer to categories B and C specifically. Copyright: by Dr Gila Olsher ...
... In this presentation we are dealing only with questions from the three modules which refer to categories B and C specifically. Copyright: by Dr Gila Olsher ...
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... radiolableled proteins present in VV-infected cells compared to those found in purified virions. ...
... radiolableled proteins present in VV-infected cells compared to those found in purified virions. ...
FEMS Microbiology Letters
... loss of zinc e¥ux and resistance. These residues are conserved in all CDF proteins and could form a charge relay system [3]. In addition, a H53R substitution also resulted in loss of resistance. His53 is present in all known zinc transporters but not in all CDF proteins. ZitB (H53M54) and CzcD both ...
... loss of zinc e¥ux and resistance. These residues are conserved in all CDF proteins and could form a charge relay system [3]. In addition, a H53R substitution also resulted in loss of resistance. His53 is present in all known zinc transporters but not in all CDF proteins. ZitB (H53M54) and CzcD both ...
Jake Wang - Critical Review of the Rosetta Algorithm
... Determining their structures has far-reaching implications in science and medicine. For example, once the structure of a protein is known, its function can be more readily elucidated. Additionally, appreciating how proteins fold can also facilitate drug design. While experimental determination of pr ...
... Determining their structures has far-reaching implications in science and medicine. For example, once the structure of a protein is known, its function can be more readily elucidated. Additionally, appreciating how proteins fold can also facilitate drug design. While experimental determination of pr ...
MODified™ Protein Domain Binding Kit Manual
... Recombinant His-tagged BRD4 (333-460) protein domain (Active Motif Catalog No. 31446) was incubated with the MODified Histone Peptide array in combination with a c-Myc antibody (Active Motif Catalog No. 39502) for control peptide P21 according to the MODified Histone Protein Domain Binding manual at ...
... Recombinant His-tagged BRD4 (333-460) protein domain (Active Motif Catalog No. 31446) was incubated with the MODified Histone Peptide array in combination with a c-Myc antibody (Active Motif Catalog No. 39502) for control peptide P21 according to the MODified Histone Protein Domain Binding manual at ...
Chapter 9
... • Nitric oxide is a mediator of immune function, a neurotransmitter, a signaling molecule, and endothelium-derived relaxing factor and other positive role in metabolism. • It is a cytotoxic free radical. • Is synthesized from arginine through action of the enzyme nitric oxide synthase (NOS). • Many ...
... • Nitric oxide is a mediator of immune function, a neurotransmitter, a signaling molecule, and endothelium-derived relaxing factor and other positive role in metabolism. • It is a cytotoxic free radical. • Is synthesized from arginine through action of the enzyme nitric oxide synthase (NOS). • Many ...
Targeting to the T. gondii plastid
... indicated constructs by suspending washed parasites in SDS-sample buffer and boiling for 2 minutes. Samples were analyzed by SDSPAGE, and immunoblotted using anti-GFP (1:10,000 dilution). Bound antibody was detected with 125I-protein A (NEN, Boston MA, USA). Protein import into chloroplasts Proteins ...
... indicated constructs by suspending washed parasites in SDS-sample buffer and boiling for 2 minutes. Samples were analyzed by SDSPAGE, and immunoblotted using anti-GFP (1:10,000 dilution). Bound antibody was detected with 125I-protein A (NEN, Boston MA, USA). Protein import into chloroplasts Proteins ...
Functional Characterization of the 180
... cyanogen bromide fragment of RRp as indicated in Fig. 1. Northern analysis of M D C K cell poly(A) + R N A using this clone as a probe revealed a single m R N A species of 5.56.0 kb in length (Fig. 2), sufficient to encode a 180-kD protein, that was at least 2-kb longer than our longest clone. All e ...
... cyanogen bromide fragment of RRp as indicated in Fig. 1. Northern analysis of M D C K cell poly(A) + R N A using this clone as a probe revealed a single m R N A species of 5.56.0 kb in length (Fig. 2), sufficient to encode a 180-kD protein, that was at least 2-kb longer than our longest clone. All e ...
The phosphorylation of proteins: a major mechanism for biological
... a third enzyme that undergoes reversible phosphorylation-dephosphorylation (Friedman & Larner, 1963). To this point the three known examples of enzyme regulation by phosphorylation-dephosphorylation, phosphorylase, phosphorylase kinase and the synthase, all involved glucogen metabolism, and it was c ...
... a third enzyme that undergoes reversible phosphorylation-dephosphorylation (Friedman & Larner, 1963). To this point the three known examples of enzyme regulation by phosphorylation-dephosphorylation, phosphorylase, phosphorylase kinase and the synthase, all involved glucogen metabolism, and it was c ...
Document
... Relate sample size to both validity and reproducibility Recognise and terms ‘mean’ ‘median’ and ‘mode’ ...
... Relate sample size to both validity and reproducibility Recognise and terms ‘mean’ ‘median’ and ‘mode’ ...
File 03
... (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). Acting as a second messenger the cGMP then acts on the muscle and causes it to relax (consequently allowing blood to enter the penis and erection takes place). However once cGMP has done its job, it is destroyed by an esterase enzyme ...
... (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). Acting as a second messenger the cGMP then acts on the muscle and causes it to relax (consequently allowing blood to enter the penis and erection takes place). However once cGMP has done its job, it is destroyed by an esterase enzyme ...
The method SPrOS (Specificity Projection On Sequence)
... identifiers of classes, to which the proteins are assigned. One and the same protein can be related to more than one class, allowing the intersected classes. The first row should contain headers. The last raw should contain the single symbol ‘#’. At least two classes must be provided. The specificit ...
... identifiers of classes, to which the proteins are assigned. One and the same protein can be related to more than one class, allowing the intersected classes. The first row should contain headers. The last raw should contain the single symbol ‘#’. At least two classes must be provided. The specificit ...
Chapter 17
... 1. Mitochondrion has outer and inner membranes. Inner compartment is called matrix where citric acid cycle and oxidative phosphorylation are carried out. 2. Inner membrane is impermeable to most hydrophilic substances, except for O2, CO2 and H2O. 3. NADH produced in cytosol by glycolysis is transpor ...
... 1. Mitochondrion has outer and inner membranes. Inner compartment is called matrix where citric acid cycle and oxidative phosphorylation are carried out. 2. Inner membrane is impermeable to most hydrophilic substances, except for O2, CO2 and H2O. 3. NADH produced in cytosol by glycolysis is transpor ...
Nature
... LeuT is a stable, sodium-coupled leucine transporter from the eubacterium Aquifex aeolicus and is the only member of the neurotransmitter sodium symporter (NSS or SLC6) family of secondary transporters that has so far been amenable to structural analysis17. Eukaryotic NSS counterparts include those ...
... LeuT is a stable, sodium-coupled leucine transporter from the eubacterium Aquifex aeolicus and is the only member of the neurotransmitter sodium symporter (NSS or SLC6) family of secondary transporters that has so far been amenable to structural analysis17. Eukaryotic NSS counterparts include those ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.