Connection of the Mitochondrial Outer and Inner Membranes by
... were treated with proteinase K. Using a specific antiserum recognizing the COOH-terminal 12 amino acid residues of Fzo1, a protease-resistant fragment of ⵑ19 kD was detected by Western blotting (Fig. 1 B, lane 2). The calculated size of a COOH-terminal Fzo1 fragment including both predicted transmem ...
... were treated with proteinase K. Using a specific antiserum recognizing the COOH-terminal 12 amino acid residues of Fzo1, a protease-resistant fragment of ⵑ19 kD was detected by Western blotting (Fig. 1 B, lane 2). The calculated size of a COOH-terminal Fzo1 fragment including both predicted transmem ...
S11. Computational Molecular Modeling- Week 5. 3
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...
Gonococcal outer-membrane protein PIB
... Solid-phase peptide synthesis. This was done using a commercially available kit (Cambridge Research Biochemicals) in which peptides are synthesized onto polyethylene rods (Geysen et al., 1987). Synthesis was done as described previously (Virji & Heckels, 1989) using pentafluorophenyl active esters o ...
... Solid-phase peptide synthesis. This was done using a commercially available kit (Cambridge Research Biochemicals) in which peptides are synthesized onto polyethylene rods (Geysen et al., 1987). Synthesis was done as described previously (Virji & Heckels, 1989) using pentafluorophenyl active esters o ...
Slide 1
... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...
... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...
51`oUL cUoa
... relative amount of plasma protein fractions but the total protein content can undergo a change depending on the different disease states. A large number of methods are available for determination of plasma proteins and they are different in their sensitivity and specificity. The commonly used spectr ...
... relative amount of plasma protein fractions but the total protein content can undergo a change depending on the different disease states. A large number of methods are available for determination of plasma proteins and they are different in their sensitivity and specificity. The commonly used spectr ...
Oxidation – a molecule loses electrons
... 4. Oxidative Phosphorylation: Electron Transport Chain and Chemiosmosis a. All of the NADH and FADH2 molecules created in glycolysis and the Citric Acid Cycle become oxidized (lose their e-, therefore recycled back to NAD+ and FAD) to the proteins in the inner membrane of the mitochondria. While the ...
... 4. Oxidative Phosphorylation: Electron Transport Chain and Chemiosmosis a. All of the NADH and FADH2 molecules created in glycolysis and the Citric Acid Cycle become oxidized (lose their e-, therefore recycled back to NAD+ and FAD) to the proteins in the inner membrane of the mitochondria. While the ...
U4L26 Nitrogen - The University of Sydney
... – Using glutamate and ammonia • Glutamine effectively carries two amino groups ...
... – Using glutamate and ammonia • Glutamine effectively carries two amino groups ...
Knowledge-Based Integration of Neuroscience Data Sources
... – compatible terms not directly joinable – complex, indirect associations among schema elements ...
... – compatible terms not directly joinable – complex, indirect associations among schema elements ...
Sources of enzyme
... acetobutylicum which is clearly involved in the microbial conversion of polysaccharides to butanol and acetone. 2) amylase produced by Aspergillus niger, Penicillium ...
... acetobutylicum which is clearly involved in the microbial conversion of polysaccharides to butanol and acetone. 2) amylase produced by Aspergillus niger, Penicillium ...
LECT24 enz2
... How does that tell me how much enzyme is present? If we relate enzyme units to micrograms of enzyme, we can immediately determine the weight of enzyme by knowing the activity. What if the enzyme is not pure? We then relate activity to mg of protein present. That measurement gives us the specific ac ...
... How does that tell me how much enzyme is present? If we relate enzyme units to micrograms of enzyme, we can immediately determine the weight of enzyme by knowing the activity. What if the enzyme is not pure? We then relate activity to mg of protein present. That measurement gives us the specific ac ...
Template-Synthesized Protein Nanotubes
... the protein-immobilization agent glutaraldehyde (GA),14,15 leaving unreacted aldehyde groups on the pore walls. The membrane is then exposed to a solution of the desired protein, which reacts via free amino sites with the aldehyde groups on the pore wall. The membrane is then alternately exposed to ...
... the protein-immobilization agent glutaraldehyde (GA),14,15 leaving unreacted aldehyde groups on the pore walls. The membrane is then exposed to a solution of the desired protein, which reacts via free amino sites with the aldehyde groups on the pore wall. The membrane is then alternately exposed to ...
Nuclear accumulation of hepatitis B virus preS fragments
... Cell lines were obtained from the ATCC (Rockville, MD). The human hepatoma cell line HepG2 and the human epitheloid carcinoma cell line HeLa were cultured in Dulbecco’s modified Eagle’s media (DMEM; GibcoBRL, Grand Island, NY) supplemented with 10% fetal bovine serum. Cells were cultured at 37°C in ...
... Cell lines were obtained from the ATCC (Rockville, MD). The human hepatoma cell line HepG2 and the human epitheloid carcinoma cell line HeLa were cultured in Dulbecco’s modified Eagle’s media (DMEM; GibcoBRL, Grand Island, NY) supplemented with 10% fetal bovine serum. Cells were cultured at 37°C in ...
After oral administration, bioactive substances and drugs must be
... Enhancement of the water solubility of bioactive substances and drugs by complexation will increase their absorption efficiency and bioavailability. This will allow reduction of the dose required to achieve the desired response, and reduction of any harmful side effects of the drug. The formation of ...
... Enhancement of the water solubility of bioactive substances and drugs by complexation will increase their absorption efficiency and bioavailability. This will allow reduction of the dose required to achieve the desired response, and reduction of any harmful side effects of the drug. The formation of ...
Analysis of the Expression Construct in Cell Lines Used
... The steps in the assembly of the expression construct should be described in detail. This description should include the source and function of the component parts of the expression construct , e.g., origins of replication, antibiotic resistance genes, promoters, enhancers, whether or not the protei ...
... The steps in the assembly of the expression construct should be described in detail. This description should include the source and function of the component parts of the expression construct , e.g., origins of replication, antibiotic resistance genes, promoters, enhancers, whether or not the protei ...
Lateral gene transfer and the evolution of plastid
... Chlorarachniophytes are amoeboflagellate algae that acquired photosynthesis secondarily by engulfing a green alga and retaining its plastid (chloroplast). An important consequence of secondary endosymbiosis in chlorarachniophytes is that most of the nuclear genes encoding plastid-targeted proteins h ...
... Chlorarachniophytes are amoeboflagellate algae that acquired photosynthesis secondarily by engulfing a green alga and retaining its plastid (chloroplast). An important consequence of secondary endosymbiosis in chlorarachniophytes is that most of the nuclear genes encoding plastid-targeted proteins h ...
6-Translation
... 6. Protein factors (initiation, elongation & release factors) 7. ATP & GTP as source of energy ...
... 6. Protein factors (initiation, elongation & release factors) 7. ATP & GTP as source of energy ...
chordin (H-300): sc-28964 - Santa Cruz Biotechnology
... chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGFβ-like bone morphogenetic proteins and sequestering them in latent complexes. Chordin dorsalizes early vertebrate embryonic tissues by binding to bone morphogenetic protei ...
... chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGFβ-like bone morphogenetic proteins and sequestering them in latent complexes. Chordin dorsalizes early vertebrate embryonic tissues by binding to bone morphogenetic protei ...
RBTopic3_6 Enzymes - wfs
... this, the reactions may occur faster. Enzymes are organic catalysts. They are proteins. 2. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. 3. The molecule ...
... this, the reactions may occur faster. Enzymes are organic catalysts. They are proteins. 2. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. 3. The molecule ...
Supplemental Text
... GSSG levels similar to or even higher than APAP alone. This indicated that the delayed GSH treatment did not prevent the mitochondrial oxidant stress, which is presumably initiated by binding of NAPQI to mitochondrial proteins.15 These data provided strong evidence that the delayed treatment with G ...
... GSSG levels similar to or even higher than APAP alone. This indicated that the delayed GSH treatment did not prevent the mitochondrial oxidant stress, which is presumably initiated by binding of NAPQI to mitochondrial proteins.15 These data provided strong evidence that the delayed treatment with G ...
Principles of Metabolic Regulation
... • Living systems thrive by keeping some metabolic reactions far from equilibrium while the levels of metabolites are in steady state ...
... • Living systems thrive by keeping some metabolic reactions far from equilibrium while the levels of metabolites are in steady state ...
do not
... How do enzymes work? 1) Enzymes act upon a substance called a substrate 2) The enzyme has an indent in it called the active site where the substrate can fit into, kind of like a lock and a key ...
... How do enzymes work? 1) Enzymes act upon a substance called a substrate 2) The enzyme has an indent in it called the active site where the substrate can fit into, kind of like a lock and a key ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.