Aphelenchoides besseyi
... Rice white tip nematode, Aphelenchoides besseyi, is a kind of plant parasitic nematodes that cause serious losses in rice and many other crops. Fatty acid and retinoid binding protein (FAR) is a specific protein in nematodes and is related to development, reproduction, infection to the host, and dis ...
... Rice white tip nematode, Aphelenchoides besseyi, is a kind of plant parasitic nematodes that cause serious losses in rice and many other crops. Fatty acid and retinoid binding protein (FAR) is a specific protein in nematodes and is related to development, reproduction, infection to the host, and dis ...
Effects of Aging on Activities of Mitochondrial Electron Transport
... Mitochondria (0.04 mg protein per ml) were incubated at 25 ºC in assay buffer containing 0.3 mM acetyl CoA and 0.1 mM 2,2-dithiobisnitrobenzoic acid (DTNB). The reaction was started by the addition of 0.5 mM oxaloacatate and reduction of DTNB was followed at 412 nm (ε=13.6 mM-1cm-1). Western blot an ...
... Mitochondria (0.04 mg protein per ml) were incubated at 25 ºC in assay buffer containing 0.3 mM acetyl CoA and 0.1 mM 2,2-dithiobisnitrobenzoic acid (DTNB). The reaction was started by the addition of 0.5 mM oxaloacatate and reduction of DTNB was followed at 412 nm (ε=13.6 mM-1cm-1). Western blot an ...
Amino acids
... – Photosynthetic reactions take place on the thylakoid membranes – Formation of carbohydrate from CO2 takes place in stroma – Much larger than mitochondria Mitochondria – Similar to animal cells, responsible for energy generation in the dark Vacuole - functions in transport and storage of nutrients ...
... – Photosynthetic reactions take place on the thylakoid membranes – Formation of carbohydrate from CO2 takes place in stroma – Much larger than mitochondria Mitochondria – Similar to animal cells, responsible for energy generation in the dark Vacuole - functions in transport and storage of nutrients ...
1 Enzymes: The Biological Catalysts Definition: Enzymes are
... of reaction catalyzed and for a single substrate or a set of closely related substrates. RNA as an Enzyme Although enzymes are considered to be proteins, enzyme activity has recently been found in ribonucleic acid (RNA) in certain organisms. Enzyme Catalysis: The enzyme (E) has a reactive site (call ...
... of reaction catalyzed and for a single substrate or a set of closely related substrates. RNA as an Enzyme Although enzymes are considered to be proteins, enzyme activity has recently been found in ribonucleic acid (RNA) in certain organisms. Enzyme Catalysis: The enzyme (E) has a reactive site (call ...
Fluorescence Study of Bovine β-Lactoglobulin
... near the pocket [1-5]. In order to find out whether both Trp residues contribute to β-lactoglobulin fluorescence or not, time-resolved studies and static quenching were performed in presence of high concentrations of calcofluor white, a fluorophore that is specific to both carbohydrate residues and ...
... near the pocket [1-5]. In order to find out whether both Trp residues contribute to β-lactoglobulin fluorescence or not, time-resolved studies and static quenching were performed in presence of high concentrations of calcofluor white, a fluorophore that is specific to both carbohydrate residues and ...
protein - Warren County Schools
... are 20 different amino acids that are used to make proteins. •If a two-letter code were used there would not be enough codons to select all 20 amino acids. •That is, there are 4 bases in RNA, so 42 (4x 4)=16; where as 43 (4x4x4)=64. ...
... are 20 different amino acids that are used to make proteins. •If a two-letter code were used there would not be enough codons to select all 20 amino acids. •That is, there are 4 bases in RNA, so 42 (4x 4)=16; where as 43 (4x4x4)=64. ...
Nucleic Acid Lateral Flow Immunoassay for the Detection of
... Abstract: Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the othe ...
... Abstract: Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the othe ...
Covalent interactions are not required to permit or stabilize the non
... cells were washed and incubated in BHK medium with reduced concentrations of NCS (2 %) and methionine (1\5 normal concentration) at 3n5 h post-electroporation. After incubation at 37 mC for 30 min, proteins were radiolabelled by addition of 10 µCi\ml [$&S]methionine (1175 Ci\mmol ; Amersham) and inc ...
... cells were washed and incubated in BHK medium with reduced concentrations of NCS (2 %) and methionine (1\5 normal concentration) at 3n5 h post-electroporation. After incubation at 37 mC for 30 min, proteins were radiolabelled by addition of 10 µCi\ml [$&S]methionine (1175 Ci\mmol ; Amersham) and inc ...
Gene Section STK4 (serine/threonine kinase 4) Atlas of Genetics and Cytogenetics
... STK4 is a member of the GC kinase group of the STE20 family of serine/threonine protein kinases. STK4 homodimerizes through a C-terminal motif, and removal of the C terminus results in marked activation of the kinase. STK4 is cleaved by caspases during apotosis, releasing an active 34 kD kinase frag ...
... STK4 is a member of the GC kinase group of the STE20 family of serine/threonine protein kinases. STK4 homodimerizes through a C-terminal motif, and removal of the C terminus results in marked activation of the kinase. STK4 is cleaved by caspases during apotosis, releasing an active 34 kD kinase frag ...
Function of Conserved Tryptophans in the Aspergillus niger
... procedure was therefore modified as described in Materials and Methods. In order to improve the amount of W615K, we also attempted to purify it according to the alternative method. Unfortunately, this did not improve the recovered amount of W615K. During the gel filtration step, elution of the prote ...
... procedure was therefore modified as described in Materials and Methods. In order to improve the amount of W615K, we also attempted to purify it according to the alternative method. Unfortunately, this did not improve the recovered amount of W615K. During the gel filtration step, elution of the prote ...
Gene regulation in physiological stress
... regulation of the assembly and binding of RNA polymerase II and a group of general transcription factors at the gene promoter as well as mechanisms of chromatin remodelling and histone modification to allow polymerase to gain access to the DNA template. Such controls often alter overall transcriptio ...
... regulation of the assembly and binding of RNA polymerase II and a group of general transcription factors at the gene promoter as well as mechanisms of chromatin remodelling and histone modification to allow polymerase to gain access to the DNA template. Such controls often alter overall transcriptio ...
Protein Production and the Genetic Code
... attach to them like clothespins clamped onto a clothesline. 3. The process of converting the information in a sequence of amino acids that make up a protein is known as translation ...
... attach to them like clothespins clamped onto a clothesline. 3. The process of converting the information in a sequence of amino acids that make up a protein is known as translation ...
Cells and Molecules of Life
... (1) While the H:O ratio in carbohydrates is about 2:1, the H:O ratio in lipids is 18:1. (Read this as: “While the hydrogen to oxygen ratio in carbohydrates is about two to one, the hydrogen to oxygen ratio in lipids is eighteen to one.”) (2) Lipids are insoluble in water, but they are soluble in org ...
... (1) While the H:O ratio in carbohydrates is about 2:1, the H:O ratio in lipids is 18:1. (Read this as: “While the hydrogen to oxygen ratio in carbohydrates is about two to one, the hydrogen to oxygen ratio in lipids is eighteen to one.”) (2) Lipids are insoluble in water, but they are soluble in org ...
mini catalog_NEW - FOTODYNE Educational Products Division
... a DNA chip using genes related to lung cancer and smoking. Students start by conducting their own clinical research survey and then viewing healthy and diseased lung ...
... a DNA chip using genes related to lung cancer and smoking. Students start by conducting their own clinical research survey and then viewing healthy and diseased lung ...
Structure–function relationships of the variable - IMGT
... usually achieved by comparing the sequences of the variable domains of the selected rat or murine monoclonal antibody to human sequences and 3D structures available in databases. It should be emphasized that the three humanized antibodies were designed using the Kabat system for defining the CDRs [1 ...
... usually achieved by comparing the sequences of the variable domains of the selected rat or murine monoclonal antibody to human sequences and 3D structures available in databases. It should be emphasized that the three humanized antibodies were designed using the Kabat system for defining the CDRs [1 ...
Structure of LEP100, a Glycoprotein That Shuttles between
... microsomes by immune precipitation followed by SDSPAGE (see Materials and Methods). The NH2-terminal amino acid sequence of LEP100, determined by sequential automated Edman degradation and product analysis, was PheAspValArgAspSefrhrGlyLysValCysIleIleAlaXLeuThrValAlaPheSerValGluTyrLysXXGlyGlnLys. Pos ...
... microsomes by immune precipitation followed by SDSPAGE (see Materials and Methods). The NH2-terminal amino acid sequence of LEP100, determined by sequential automated Edman degradation and product analysis, was PheAspValArgAspSefrhrGlyLysValCysIleIleAlaXLeuThrValAlaPheSerValGluTyrLysXXGlyGlnLys. Pos ...
New Options for patients with imatinib
... Signalling pathways and targets involved in SCCHN and their potential inhibitors ...
... Signalling pathways and targets involved in SCCHN and their potential inhibitors ...
King Saud University
... 7. Entering QC data, Levy-Jennings Chart, etc. 8. Statistics for medical research and workload. ...
... 7. Entering QC data, Levy-Jennings Chart, etc. 8. Statistics for medical research and workload. ...
BrevdueNord.dk Additional Thoughts on Nutrition for Racing Gordon
... these scientists to track the glucose and determine its fate in the body), was incorporated into fatty acids in the liver within three minutes in hungry young pigeons, and that the content of fatty acids in liver reached a plateau in 15 minutes. Significant appearance of fatty acids in blood and fat ...
... these scientists to track the glucose and determine its fate in the body), was incorporated into fatty acids in the liver within three minutes in hungry young pigeons, and that the content of fatty acids in liver reached a plateau in 15 minutes. Significant appearance of fatty acids in blood and fat ...
A system in mouse liver for the repair of O6
... rapid. At an intermediate temperature of 20°C, 0 -methylguanine dissappeared at a much slower rate than at 37°C. It is noteworthy that after 60 min of incubation time, the amount of 0 -methylguanine released at 20°C approached the value achieved at 37°C. The effect of initial substrate concentration ...
... rapid. At an intermediate temperature of 20°C, 0 -methylguanine dissappeared at a much slower rate than at 37°C. It is noteworthy that after 60 min of incubation time, the amount of 0 -methylguanine released at 20°C approached the value achieved at 37°C. The effect of initial substrate concentration ...
Zinc Finger Folding Activity
... beta sheet. The structure of the zinc finger is stabilized by a zinc atom that binds 2 cysteine and 2 histidine sidechains, and by hydrophobic amino acid sidechains that are buried on the inside of the folded motif. Zinc finger proteins function as regulators of gene expression. They bind to the neg ...
... beta sheet. The structure of the zinc finger is stabilized by a zinc atom that binds 2 cysteine and 2 histidine sidechains, and by hydrophobic amino acid sidechains that are buried on the inside of the folded motif. Zinc finger proteins function as regulators of gene expression. They bind to the neg ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.