Dynamic in vivo interactions among Myc network members
... mad1 and mxi1 nullizygous mice have demonstrated distinct roles for the encoded proteins in tissue-speci®c negative control of cellular proliferation (Foley et al., 1998; Schreiber-Agus et al., 1998). Despite the well-described interactions of Myc network proteins, little is known about their distri ...
... mad1 and mxi1 nullizygous mice have demonstrated distinct roles for the encoded proteins in tissue-speci®c negative control of cellular proliferation (Foley et al., 1998; Schreiber-Agus et al., 1998). Despite the well-described interactions of Myc network proteins, little is known about their distri ...
Differential localization of LTA synthesis proteins and their
... variety of Gram-positive bacteria and its synthesis and function has been studied most extensively in S. aureus (Fischer, 1990; Rahman et al., 2009; Reichmann and Gründling, 2011). In this organism, the PGP backbone chain is decorated with D-alanine esters and linked to the outside of the membrane t ...
... variety of Gram-positive bacteria and its synthesis and function has been studied most extensively in S. aureus (Fischer, 1990; Rahman et al., 2009; Reichmann and Gründling, 2011). In this organism, the PGP backbone chain is decorated with D-alanine esters and linked to the outside of the membrane t ...
Analysis of Protein Phosphorylation Using Mass Spectrometry
... Conventionally, radioactive 32P is introduced into cellular proteins via labeled ATP to trace phosphorylation. Fractionation techniques, such as high-performance liquid chromatography and one or two dimensional gel electrophoreses are utilized subsequently for the detection of the radioactive protei ...
... Conventionally, radioactive 32P is introduced into cellular proteins via labeled ATP to trace phosphorylation. Fractionation techniques, such as high-performance liquid chromatography and one or two dimensional gel electrophoreses are utilized subsequently for the detection of the radioactive protei ...
Enzymes
... 3 Enzymes reduce activation energy by holding substrate in a way which causes reaction to occur more easily forming enzyme-product complex ...
... 3 Enzymes reduce activation energy by holding substrate in a way which causes reaction to occur more easily forming enzyme-product complex ...
TD12 Characterization of DnaJ substrate specificity Reference
... DnaJ transfer onto PVDF membrane. Once there, it is stuck for good. Block PVDF membrane with serum or milk (Passivate the surface of the membrane, so it is no longer sticky) Add anti-DnaJ antibody, it binds to DnaJ Rinse off unbound antibody Add anti-rabbit secondary antibody conjugated to alkaline ...
... DnaJ transfer onto PVDF membrane. Once there, it is stuck for good. Block PVDF membrane with serum or milk (Passivate the surface of the membrane, so it is no longer sticky) Add anti-DnaJ antibody, it binds to DnaJ Rinse off unbound antibody Add anti-rabbit secondary antibody conjugated to alkaline ...
From DNA to Protein
... • messenger RNA (mRNA) – codes for proteins • ribosomal RNA (rRNA) – forms the core of the ribosomes, machinery for making proteins • transfer RNA (tRNA) – carries the amino acid for the growing protein chain DNA Transcription in Bacteria • RNA polymerase must know where the start of a gene is in or ...
... • messenger RNA (mRNA) – codes for proteins • ribosomal RNA (rRNA) – forms the core of the ribosomes, machinery for making proteins • transfer RNA (tRNA) – carries the amino acid for the growing protein chain DNA Transcription in Bacteria • RNA polymerase must know where the start of a gene is in or ...
RNA analysis on non-denaturing agarose gel electrophoresis
... 2. Heat an aliquot of the RNA solution at 70°C for 1 min and place it on ice before loading on a gel. 3. Load a known amount of DNA or RNA ladder alongside your RNA sample as a standard for determining the RNA concentration. RNA concentration can be roughly estimated assuming that the efficiency of ...
... 2. Heat an aliquot of the RNA solution at 70°C for 1 min and place it on ice before loading on a gel. 3. Load a known amount of DNA or RNA ladder alongside your RNA sample as a standard for determining the RNA concentration. RNA concentration can be roughly estimated assuming that the efficiency of ...
Analysis of the Nitrous Oxide Reduction Genes, nosZDFYL, of
... dimeric N2O reductases. The relevant DNA region of about 3.6 kbp was also sequenced and found to consist of four genes, nosDFYL based on the similarity with the N2O reduction genes of Pseudomonas stutzeri. The gene product of A. cycloclastes nosF (299 amino acid residues) has a consensus ATP-binding ...
... dimeric N2O reductases. The relevant DNA region of about 3.6 kbp was also sequenced and found to consist of four genes, nosDFYL based on the similarity with the N2O reduction genes of Pseudomonas stutzeri. The gene product of A. cycloclastes nosF (299 amino acid residues) has a consensus ATP-binding ...
Struct Bio 101
... A single molecule is a very weak scatterer of X-rays. Most of the X-rays will pass through the molecule without being diffracted. Those rays which are diffracted are too weak to be detected. Solution: Analyzing diffraction from crystals instead of single molecules. A crystal is made of a three-dimen ...
... A single molecule is a very weak scatterer of X-rays. Most of the X-rays will pass through the molecule without being diffracted. Those rays which are diffracted are too weak to be detected. Solution: Analyzing diffraction from crystals instead of single molecules. A crystal is made of a three-dimen ...
Presentation
... of 1 amino acid substitution per 100 amino acids. BLOSUM X: all sequences with a similarity higher than X were summarized into one ...
... of 1 amino acid substitution per 100 amino acids. BLOSUM X: all sequences with a similarity higher than X were summarized into one ...
Sequence
... all, the signal-to-noise ratio is greatly improved for the specific purpose of identifying protein relatives. It is accepted that convergence phenomena in aa sequences are very rare and thus aa similarity almost always means homology. Furthermore, aa sequences may still show a similarity derived fro ...
... all, the signal-to-noise ratio is greatly improved for the specific purpose of identifying protein relatives. It is accepted that convergence phenomena in aa sequences are very rare and thus aa similarity almost always means homology. Furthermore, aa sequences may still show a similarity derived fro ...
Molecular characterisation of RecQ homologues in Arabidopsis
... After electrophoresis in 0.9% agarose the gel was denatured, neutralised, and transferred overnight onto a Hybond-NX nylon membrane (Amersham, Braunschweig) by capillary transfer using 20× SSC as transfer buffer. After blotting, the membrane was cross-linked with 120 mJ/cm2 in a Stratalinker 1800 (S ...
... After electrophoresis in 0.9% agarose the gel was denatured, neutralised, and transferred overnight onto a Hybond-NX nylon membrane (Amersham, Braunschweig) by capillary transfer using 20× SSC as transfer buffer. After blotting, the membrane was cross-linked with 120 mJ/cm2 in a Stratalinker 1800 (S ...
Differential Accumulation Pattern of Met-rich beta
... normal, produce flowers and can be nodulated ...
... normal, produce flowers and can be nodulated ...
... biochemical reaction has an energy barrier that has to be overcome for the reaction to take place. The energy barrier of biochemical reactions prevents them from occurring spontaneously, but an enzyme as a catalyst may bring about the reaction within a cell or organism. Through their catalytic actio ...
Document
... Why not XML? - XML is a widely used standard format for corpora annotation and is supported by a large number of tools. • The processing of complex annotations in XML – parsing, storing, querying, evaluation – is usually virtually impossible with off-the-shelf XML tools. • Developers need to develop ...
... Why not XML? - XML is a widely used standard format for corpora annotation and is supported by a large number of tools. • The processing of complex annotations in XML – parsing, storing, querying, evaluation – is usually virtually impossible with off-the-shelf XML tools. • Developers need to develop ...
Lecture 13 – Endocrine System 1
... polypeptides/small proteins – this is a large & diverse group of hormones which range in size from short chain polypeptides such as oxytocin & vasopressin, to small proteins which includes such hormones as growth hormone & prolactin. This group of hormones includes all hormones secreted by the hypot ...
... polypeptides/small proteins – this is a large & diverse group of hormones which range in size from short chain polypeptides such as oxytocin & vasopressin, to small proteins which includes such hormones as growth hormone & prolactin. This group of hormones includes all hormones secreted by the hypot ...
Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in
... When comparing the protein pattern of our preparation of yeast mitochondrial outer membranes (Fig. 1, l a n e / ) with that of Riezman ct al. (1983a), MOM72 appeared to be very similar in size and abundance to a 70-kD protein that had been used as a tool to study the biogenesis of mitochondrial oute ...
... When comparing the protein pattern of our preparation of yeast mitochondrial outer membranes (Fig. 1, l a n e / ) with that of Riezman ct al. (1983a), MOM72 appeared to be very similar in size and abundance to a 70-kD protein that had been used as a tool to study the biogenesis of mitochondrial oute ...
Studying Protein Flexibility in a Statistical Framework: Tools and
... structural alignments on version 1.39 of the scop database, which represented most the known structures as of the beginning of 1999. From this they found 4403 pairs of domains that had appreciable sequence similarity yet had great structural differences; these represented putative instances of confo ...
... structural alignments on version 1.39 of the scop database, which represented most the known structures as of the beginning of 1999. From this they found 4403 pairs of domains that had appreciable sequence similarity yet had great structural differences; these represented putative instances of confo ...
Allosteric Enzymes
... -chymotrypsin catalyzes the hydrolysis of two dipeptide fragments to give -chymotrypsin -chymotrypsin consists of three polypeptide chains joined by two of the five original disulfide bonds The X-ray crystallography of chymotrypsin has been determined The Protonated isoleucine side chain is in ...
... -chymotrypsin catalyzes the hydrolysis of two dipeptide fragments to give -chymotrypsin -chymotrypsin consists of three polypeptide chains joined by two of the five original disulfide bonds The X-ray crystallography of chymotrypsin has been determined The Protonated isoleucine side chain is in ...
Imunoglobulins
... IgM normally exists as a pentamer. So it has the highest molecular weight. The five units are linked by small polypeptide chain called J chain 2. Properties a) IgM is the third most common serum Ig (5-10% of total Ig). b) IgM i Times New Roman s the first Ig be made by a virgin B cells when it is st ...
... IgM normally exists as a pentamer. So it has the highest molecular weight. The five units are linked by small polypeptide chain called J chain 2. Properties a) IgM is the third most common serum Ig (5-10% of total Ig). b) IgM i Times New Roman s the first Ig be made by a virgin B cells when it is st ...
Slide 1
... • Proteins contain the elements C H O N & sometimes S • They are made by condensation reactions between amino acids forming long polypeptide chains. • The properties of each individual protein are determined by the aa sequence ...
... • Proteins contain the elements C H O N & sometimes S • They are made by condensation reactions between amino acids forming long polypeptide chains. • The properties of each individual protein are determined by the aa sequence ...
Biological Science, 5e (Freeman) Chapter 3 Protein Structure and
... Since structure correlates well with function, look for new ways to probe the complex structure of proteins in order to understand what they do and how they do it. One of the most powerful techniques in existence today is X-ray crystallography. The main difficulty with this technique is getting the ...
... Since structure correlates well with function, look for new ways to probe the complex structure of proteins in order to understand what they do and how they do it. One of the most powerful techniques in existence today is X-ray crystallography. The main difficulty with this technique is getting the ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.