22. Think of two different proteins: both are enzymes. a) What
... Phosphorylation/Dephosphorylation: requires donor of phosphate group (ATP=P1+energy) d) What is the main difference between the mechanism in c) and allosteric control? Two enzymes are needed to regulate for covalent binding in phosphorylation/de. and no enzymes are used for non covalent allosteric b ...
... Phosphorylation/Dephosphorylation: requires donor of phosphate group (ATP=P1+energy) d) What is the main difference between the mechanism in c) and allosteric control? Two enzymes are needed to regulate for covalent binding in phosphorylation/de. and no enzymes are used for non covalent allosteric b ...
PCR-based gene synthesis to produce recombinant proteins for
... purified using the QIAquick PCR purification kit (Qiagen, Valencia, CA, USA), blunt-ended with Klenow fragment (Promega, Madison, WI, USA) according to the manufacturer's directions and further purified as described above. The resulting pET3a fragment was used for homologous recombination reactions ...
... purified using the QIAquick PCR purification kit (Qiagen, Valencia, CA, USA), blunt-ended with Klenow fragment (Promega, Madison, WI, USA) according to the manufacturer's directions and further purified as described above. The resulting pET3a fragment was used for homologous recombination reactions ...
SUPPLEMENTARY MATERIAL Title: Luteolin attenuate the D
... the protein quantities of each sample were determined by a BCA Protein Assay kit ((ibid.) Equal amounts of protein (100 μg) were isolated by 10% SDS-polyacrylamide gel electrophoresis and were electrophoretically transferred to a nitrocellulose membrane. The membrane was blocked with 5% skim milk po ...
... the protein quantities of each sample were determined by a BCA Protein Assay kit ((ibid.) Equal amounts of protein (100 μg) were isolated by 10% SDS-polyacrylamide gel electrophoresis and were electrophoretically transferred to a nitrocellulose membrane. The membrane was blocked with 5% skim milk po ...
Gene Section S100A4 (S100 calcium binding protein A4)
... and nuclear location. Differential distribution has been reported between stromal components of primary and metastatic tumour. Patterns of distribution could vary between tissues as well between species. No firm functional link has been made with the site/s of localisation. Expression patterns need ...
... and nuclear location. Differential distribution has been reported between stromal components of primary and metastatic tumour. Patterns of distribution could vary between tissues as well between species. No firm functional link has been made with the site/s of localisation. Expression patterns need ...
report on HMM
... weak bonds between the bases on each strand, forming base pairs (bp). Genome size is usually stated as the total number of base pairs; the human genome contains roughly 3 billion bp. A gene is a segment of a DNA molecule (ranging from fewer than 1 thousand bases to several million), located in a par ...
... weak bonds between the bases on each strand, forming base pairs (bp). Genome size is usually stated as the total number of base pairs; the human genome contains roughly 3 billion bp. A gene is a segment of a DNA molecule (ranging from fewer than 1 thousand bases to several million), located in a par ...
Selective Isotope-Labeling Methods for Protein Structural Studies
... or site-selective labeling is often pursued as it helps in spectral simplification and provides specific probes for structural and dynamic studies. Selective amino acid-type labeling also aids in sequence-specific resonance assignments by helping to identify resonances which are otherwise buried in ...
... or site-selective labeling is often pursued as it helps in spectral simplification and provides specific probes for structural and dynamic studies. Selective amino acid-type labeling also aids in sequence-specific resonance assignments by helping to identify resonances which are otherwise buried in ...
Coenzymes
... • Participates in acyl-group transfer reactions with carboxylic acids and fatty acids • CoA-dependent reactions include oxidation of fuel molecules and biosynthesis of carboxylic acids and fatty acids • Acyl groups are covalently attached to the -SH of CoA to form thioesters ...
... • Participates in acyl-group transfer reactions with carboxylic acids and fatty acids • CoA-dependent reactions include oxidation of fuel molecules and biosynthesis of carboxylic acids and fatty acids • Acyl groups are covalently attached to the -SH of CoA to form thioesters ...
Paper Title
... position in a DNA among individuals. Significant number of genetic diseases is caused by nonsynonymous SNPs manifested as single point mutations on the protein level. The ability to identify deleterious substitutions could be useful for protein engineering to test whether the proposed mutations do n ...
... position in a DNA among individuals. Significant number of genetic diseases is caused by nonsynonymous SNPs manifested as single point mutations on the protein level. The ability to identify deleterious substitutions could be useful for protein engineering to test whether the proposed mutations do n ...
Macromolecules II PDF
... linked by the–OH group on the 3´ carbon of one nucleotide and the phosphate on the 5´ carbon on the next ...
... linked by the–OH group on the 3´ carbon of one nucleotide and the phosphate on the 5´ carbon on the next ...
Functional analysis of the human CDC5L complex
... and analysed its interaction with other protein factors in HeLa nuclear extract. We show that the catalytic steps of splicing are inhibited (with the second catalytic step being more sensitive) upon immunodepletion of CDC5L from HeLa nuclear extracts, whereas spliceosome assembly still occurs. Splic ...
... and analysed its interaction with other protein factors in HeLa nuclear extract. We show that the catalytic steps of splicing are inhibited (with the second catalytic step being more sensitive) upon immunodepletion of CDC5L from HeLa nuclear extracts, whereas spliceosome assembly still occurs. Splic ...
PDF Datastream - Brown Digital Repository
... bacterial processes such as cell wall biogenesis as well as the promise of finding potential vaccine- or drug-targets for virulent bacteria. Meanwhile, rapid development in mass spectrometry based proteomics, especially quantitative analysis of targeted proteome, received increased research interest ...
... bacterial processes such as cell wall biogenesis as well as the promise of finding potential vaccine- or drug-targets for virulent bacteria. Meanwhile, rapid development in mass spectrometry based proteomics, especially quantitative analysis of targeted proteome, received increased research interest ...
Biochemistry of connective tissue
... large elastic blood vessels such as the aorta. Elastin is also very important in the lungs, elastic ligaments, the skin, the bladder, elastic cartilage ...
... large elastic blood vessels such as the aorta. Elastin is also very important in the lungs, elastic ligaments, the skin, the bladder, elastic cartilage ...
The hnRNP C Proteins Contain a Nuclear Retention Sequence That
... HeLa cells were transfected with DNA encoding the epitopetagged protein of interest, and then fused to mouse NIH 3T3 cells to form heterokaryons, in which human and mouse nuclei share a common cytoplasm. 4 h after fusion, heterokaryons were fixed and stained for immunofluorescence microscopy to exam ...
... HeLa cells were transfected with DNA encoding the epitopetagged protein of interest, and then fused to mouse NIH 3T3 cells to form heterokaryons, in which human and mouse nuclei share a common cytoplasm. 4 h after fusion, heterokaryons were fixed and stained for immunofluorescence microscopy to exam ...
The Genome of Methanosarcina mazei
... the already mentioned non-coding regions (1.01 Mbp) and by 3,371 ORFs as compared to 1,738 identified ORFs in Mc. jannaschii (Bult et al., 1996). A striking feature of the M. mazei genome is that 1,043 of the 3,371 ORFs have their best blast scores outside the genus Methanosarcina in the bacterial d ...
... the already mentioned non-coding regions (1.01 Mbp) and by 3,371 ORFs as compared to 1,738 identified ORFs in Mc. jannaschii (Bult et al., 1996). A striking feature of the M. mazei genome is that 1,043 of the 3,371 ORFs have their best blast scores outside the genus Methanosarcina in the bacterial d ...
GPCR–G fusion proteins
... Construction of fusion protein DNAs and structural properties of fusion proteins Fusion proteins are generated by linking the GPCR C-terminus, which is located intracellularly, to the Nterminus of Ga (Refs 1–6, 9–13, 18). This is achieved by fusing the open reading frames of the two proteins using D ...
... Construction of fusion protein DNAs and structural properties of fusion proteins Fusion proteins are generated by linking the GPCR C-terminus, which is located intracellularly, to the Nterminus of Ga (Refs 1–6, 9–13, 18). This is achieved by fusing the open reading frames of the two proteins using D ...
Review Questions for Advanced Biochemistry Course
... A. This is one means by which the TCA cycle is linked to the electron-transport chain B. Succinate dehydrogenase is embedded in the inner mitochondrial membrane C. Neither the single- or 2-electron forms of FAD dissociate from the enzyme D. Complex II transfers its electrons directly to cytochrome c ...
... A. This is one means by which the TCA cycle is linked to the electron-transport chain B. Succinate dehydrogenase is embedded in the inner mitochondrial membrane C. Neither the single- or 2-electron forms of FAD dissociate from the enzyme D. Complex II transfers its electrons directly to cytochrome c ...
Strategy for Nonenveloped Virus Entry
... SDS-PAGE and immunoblotting. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was carried out on 10% acrylamide gels as described previously (51). Viral proteins were detected by staining with Coomassie brilliant blue R-250 (Sigma) or were transferred to a nitrocellulose membrane ...
... SDS-PAGE and immunoblotting. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was carried out on 10% acrylamide gels as described previously (51). Viral proteins were detected by staining with Coomassie brilliant blue R-250 (Sigma) or were transferred to a nitrocellulose membrane ...
Electrophoresis, Blotting and Immunodetection Gel
... Extract DNA from agarose gels in a single 10min spin with this easy-to-use kit. A gel nebuliser converts agarose to a spray from which DNA is separated (via a 0.45µm Ultrafree-MC filter). Prepares gel-purified PCR products for sequencing or cloning without need for further purification. Device volum ...
... Extract DNA from agarose gels in a single 10min spin with this easy-to-use kit. A gel nebuliser converts agarose to a spray from which DNA is separated (via a 0.45µm Ultrafree-MC filter). Prepares gel-purified PCR products for sequencing or cloning without need for further purification. Device volum ...
Nutrient Profile of Renal Specific Formula
... recommended to restrict fluids (water, coffee, tea, etc.) which is intricately linked with salt/sodium ...
... recommended to restrict fluids (water, coffee, tea, etc.) which is intricately linked with salt/sodium ...
PDF 28 - The Open University
... Unless otherwise stated, this resource is released under the terms of the Creative Commons Licence v4.0 http://creativecommons.org/licenses/by-nc-sa/4.0/deed.en_GB. Within that The Open University interprets this licence in the following way: www.open.edu/openlearn/about-openlearn/frequently-asked-q ...
... Unless otherwise stated, this resource is released under the terms of the Creative Commons Licence v4.0 http://creativecommons.org/licenses/by-nc-sa/4.0/deed.en_GB. Within that The Open University interprets this licence in the following way: www.open.edu/openlearn/about-openlearn/frequently-asked-q ...
PBHS AP Biology
... Sumner and the molecular weight worked out in 1938. In 1969 the amino acid sequence of bovine catalase was worked out. Then in 1981, the 3D structure of the protein was revealed. Hydrogen peroxide is a harmful by-product of many normal metabolic processes: To prevent damage, it must be quickly c ...
... Sumner and the molecular weight worked out in 1938. In 1969 the amino acid sequence of bovine catalase was worked out. Then in 1981, the 3D structure of the protein was revealed. Hydrogen peroxide is a harmful by-product of many normal metabolic processes: To prevent damage, it must be quickly c ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.