Targeted Proteomic Study of the Cyclin
... Clb3 (Supplemental Figure S1 on Molecular Cell’s website) was observed, most likely because of its role in targeting this cyclin for degradation (Schwab et al., 2001; Visintin et al., 1997). The association of Cdc4 and Cdc53 with Clb3 (Supplemental Figure S1 on Molecular Cell’s website), could have ...
... Clb3 (Supplemental Figure S1 on Molecular Cell’s website) was observed, most likely because of its role in targeting this cyclin for degradation (Schwab et al., 2001; Visintin et al., 1997). The association of Cdc4 and Cdc53 with Clb3 (Supplemental Figure S1 on Molecular Cell’s website), could have ...
C.N.R. Short-term Mobility Program 2008 Gabriella Pocsfalvi
... DNA uptake as well. Nonetheless flagellation in archaea is widespread, structural and functional characterization of archaeal flagella today is still far less comprehensive than bacterial flagella or pili. The fla operon of S. solfataricus encodes seven open reading frames: five putative accessory p ...
... DNA uptake as well. Nonetheless flagellation in archaea is widespread, structural and functional characterization of archaeal flagella today is still far less comprehensive than bacterial flagella or pili. The fla operon of S. solfataricus encodes seven open reading frames: five putative accessory p ...
Molecular Mechanisms of Transforming Growth Factor
... Five TGF-b ligands have been cloned (TGF-b1–5). These ligands are secreted as 100-kDa inactive complexes. The inactive form consists of a dimer of the N-terminal peptide, noncovalently associated with the 25-kDa dimer of the biologically active form. Structurally, the ligands exhibit a high degree o ...
... Five TGF-b ligands have been cloned (TGF-b1–5). These ligands are secreted as 100-kDa inactive complexes. The inactive form consists of a dimer of the N-terminal peptide, noncovalently associated with the 25-kDa dimer of the biologically active form. Structurally, the ligands exhibit a high degree o ...
Gene Section MIER1 (mesoderm induction early response 1 homolog (Xenopus laevis))
... of the MIER1 isoforms are followed by common internal sequence containing several distinct domains: acidic, which function in transcriptional activation (Paterno et al., 1997); ELM2, responsible for recruitment of HDAC1 (Ding et al., 2003); SANT, which interacts with Sp1 (Ding et al., 2004) and PSPP ...
... of the MIER1 isoforms are followed by common internal sequence containing several distinct domains: acidic, which function in transcriptional activation (Paterno et al., 1997); ELM2, responsible for recruitment of HDAC1 (Ding et al., 2003); SANT, which interacts with Sp1 (Ding et al., 2004) and PSPP ...
Schizosaccharomyces pombe Git1 Is a C2
... unknown function as well as the UNC-13/Munc13 protein family involved in synaptic vesicle exocytosis. All of these proteins contain a similar C2 domain near their carboxy-terminus, along with two additional domains known as Munc13-homology domains 1 and 2 (Koch et al. 2000). C2 domains are often uti ...
... unknown function as well as the UNC-13/Munc13 protein family involved in synaptic vesicle exocytosis. All of these proteins contain a similar C2 domain near their carboxy-terminus, along with two additional domains known as Munc13-homology domains 1 and 2 (Koch et al. 2000). C2 domains are often uti ...
Alight-inducible organelle targeting system for dynamically
... cyclin function, we deleted CLB1 and directly tagged endogenous CLB2 with PIF at its C-terminus. By tagging cyclin at the endogenous locus, we gain the ability for optical control of the spatial and temporal dynamics of the sole M-phase cyclin. This enables us to control when and where cyclin is act ...
... cyclin function, we deleted CLB1 and directly tagged endogenous CLB2 with PIF at its C-terminus. By tagging cyclin at the endogenous locus, we gain the ability for optical control of the spatial and temporal dynamics of the sole M-phase cyclin. This enables us to control when and where cyclin is act ...
An artificial transcription activator mimics the genomewide
... domain of Gal4. The chimerical protein was designed to contain three N-terminal HA epitopes and the SV40 nuclear targeting signal (NLS). This chimerical gene was conditionally expressed under the control of the GAL1-10 promoter. (B) Time course expression of the protein Pdr1*GAD after galactose indu ...
... domain of Gal4. The chimerical protein was designed to contain three N-terminal HA epitopes and the SV40 nuclear targeting signal (NLS). This chimerical gene was conditionally expressed under the control of the GAL1-10 promoter. (B) Time course expression of the protein Pdr1*GAD after galactose indu ...
Using Gene Expression Noise to Understand Gene Regulation
... quantifying these deviations reveal the mechanism of regulation? A key parameter to quantify the deviation from Poisson statistics is the Fano factor, which is the ratio between the variance, s2, and the mean, m, of the mRNA copy-number distribution, s2 =m (8). For a Poisson distribution, the Fano f ...
... quantifying these deviations reveal the mechanism of regulation? A key parameter to quantify the deviation from Poisson statistics is the Fano factor, which is the ratio between the variance, s2, and the mean, m, of the mRNA copy-number distribution, s2 =m (8). For a Poisson distribution, the Fano f ...
Systems biology of virus-host signaling network
... MAPK signaling (22), resulting in defective antigen presentation. These studies focused on a single pathway, however the extent of viral-mediated changes could be more fully understood by measuring changes across multiple pathways in the network and using computational methods to analyze the experim ...
... MAPK signaling (22), resulting in defective antigen presentation. These studies focused on a single pathway, however the extent of viral-mediated changes could be more fully understood by measuring changes across multiple pathways in the network and using computational methods to analyze the experim ...
Toll-like receptor signaling in cell proliferation and survival
... damaged or inflamed self tissues. Upon sensing these molecules, TLRs initiate a series of downstream signaling events that drive cellular responses including the production of cytokines, chemokines, and other inflammatory mediators. This outcome results from the intracellular assembly of protein compl ...
... damaged or inflamed self tissues. Upon sensing these molecules, TLRs initiate a series of downstream signaling events that drive cellular responses including the production of cytokines, chemokines, and other inflammatory mediators. This outcome results from the intracellular assembly of protein compl ...
Intercellular Communication during Plant
... of xylem cell types. As mentioned above, SHR mRNA is expressed in the stele, but the protein is found in the stele and endodermal cell nuclei (Helariutta et al., 2000; Nakajima et al., 2001). In the endodermis, SHR and one of its direct targets, SCARECROW (SCR), are necessary for endodermal specific ...
... of xylem cell types. As mentioned above, SHR mRNA is expressed in the stele, but the protein is found in the stele and endodermal cell nuclei (Helariutta et al., 2000; Nakajima et al., 2001). In the endodermis, SHR and one of its direct targets, SCARECROW (SCR), are necessary for endodermal specific ...
- The University of Liverpool Repository
... regeneration in damaged axon and sprouting of neighbouring axons ...
... regeneration in damaged axon and sprouting of neighbouring axons ...
Up to the minute project summary:
... Interestingly, while the Zscan protein cannot be detected until day 3, the message can be detected by RTPCR at day 0. A third unknown product of approximately 500 bases was also observed which we have not as yet sequenced. Eventually, sequence data will provide information on the function of this s ...
... Interestingly, while the Zscan protein cannot be detected until day 3, the message can be detected by RTPCR at day 0. A third unknown product of approximately 500 bases was also observed which we have not as yet sequenced. Eventually, sequence data will provide information on the function of this s ...
Direct Interaction between Rab3b and the Polymeric
... endogenous rab3b. We therefore constructed and stably transfected myc-tagged rab3b into MDCK cells. Addition of an epitope tag to the N terminus of the rab protein does not affect its subcellular localization and activity (Sönnichsen et al., 2000). Confocal microscopy revealed that myc-rab3b locali ...
... endogenous rab3b. We therefore constructed and stably transfected myc-tagged rab3b into MDCK cells. Addition of an epitope tag to the N terminus of the rab protein does not affect its subcellular localization and activity (Sönnichsen et al., 2000). Confocal microscopy revealed that myc-rab3b locali ...
Gene Expression and Signal Transduction
... seed color, and seed shape, came to be called genes. Genes are the DNA sequences that encode the RNA molecules directly involved in making the enzymes and structural proteins of the cell. Genes are arranged linearly on chromosomes, which form linkage groups—that is, genes that are inherited together ...
... seed color, and seed shape, came to be called genes. Genes are the DNA sequences that encode the RNA molecules directly involved in making the enzymes and structural proteins of the cell. Genes are arranged linearly on chromosomes, which form linkage groups—that is, genes that are inherited together ...
title page
... CKB2 YOR039w Beta' regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB1, the many substrates include transcription factors and all RNA polymerases CLN3 YAL040c G1 cyclin involved in cell cycle pr ...
... CKB2 YOR039w Beta' regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB1, the many substrates include transcription factors and all RNA polymerases CLN3 YAL040c G1 cyclin involved in cell cycle pr ...
Gene Section NLRC4 (NLR Family, CARD domain containing 4)
... 3.370kb with an ORF encoding 1024 amino acids. CLAN-B, C and D have exon 4 spliced selectively to other exons forming shorter transcripts. Tumor suppressor p53 activates transcription of full length NLRC4 mRNA by binding to a site in the minimal promoter. ...
... 3.370kb with an ORF encoding 1024 amino acids. CLAN-B, C and D have exon 4 spliced selectively to other exons forming shorter transcripts. Tumor suppressor p53 activates transcription of full length NLRC4 mRNA by binding to a site in the minimal promoter. ...
Supplementary data
... functions as the ATPase subunit of this complex which is strongly related to the yeast SWI/SNF nucleosome remodeling complex. The TrxG proteins Ash1 and TRX exist in the other two distinct multiprotein complexes. Both are SET-domain proteins and function as histone methyltarnsferase. Ash1 selectivel ...
... functions as the ATPase subunit of this complex which is strongly related to the yeast SWI/SNF nucleosome remodeling complex. The TrxG proteins Ash1 and TRX exist in the other two distinct multiprotein complexes. Both are SET-domain proteins and function as histone methyltarnsferase. Ash1 selectivel ...
Plant hormone receptors: perception is everything
... gene products have been shown to bind cytokinin and complement yeast and bacterial two-component mutants in a cytokinin-dependent manner (Fig. 3A). In Arabidopsis, there are five genes that encode HPt proteins, designated AHPs, and it appears that these intermediates transmit the membrane perceived ...
... gene products have been shown to bind cytokinin and complement yeast and bacterial two-component mutants in a cytokinin-dependent manner (Fig. 3A). In Arabidopsis, there are five genes that encode HPt proteins, designated AHPs, and it appears that these intermediates transmit the membrane perceived ...
mbn cells results in dysregulation of Fer1HCH expression
... orthologues of Mrs3/4p. CG4963 was the only probable candidate retrieved (bit scores 205 and 191 and E-values 3 × 10−53 and 3 × 10−49 respectively). The alignment of CG4963’s amino acid sequence with yeast Mrs3/4p and zebrafish mfrn1 and mfrn2 showed a ∼ 10 % higher degree of sequence conservation b ...
... orthologues of Mrs3/4p. CG4963 was the only probable candidate retrieved (bit scores 205 and 191 and E-values 3 × 10−53 and 3 × 10−49 respectively). The alignment of CG4963’s amino acid sequence with yeast Mrs3/4p and zebrafish mfrn1 and mfrn2 showed a ∼ 10 % higher degree of sequence conservation b ...
MOLECULAR AND CELLULAR BIOLOGY
... The microtubule-associated protein tau normally functions to bind to and stabilize microtubules. However, evidence now indicates that tau may also play a critical role in signaling pathways linked to neuronal development and neurodegeneration. The tau association with numerous signaling proteins suc ...
... The microtubule-associated protein tau normally functions to bind to and stabilize microtubules. However, evidence now indicates that tau may also play a critical role in signaling pathways linked to neuronal development and neurodegeneration. The tau association with numerous signaling proteins suc ...
Identification of Genes Interacting with rnt-1 Through Large
... It is well-known that a major function of RUNX genes in cells is to regulate the balance between cell proliferation and differentiation (Coffman 2003). Because the decision between cell proliferation and differentiation must reflect the precise cellular environment, the roles and action mechanism of ...
... It is well-known that a major function of RUNX genes in cells is to regulate the balance between cell proliferation and differentiation (Coffman 2003). Because the decision between cell proliferation and differentiation must reflect the precise cellular environment, the roles and action mechanism of ...
Role of glypican 4 in the regulation of convergent extension
... residues. Xenopus activin (Thomsen et al., 1990), Xnr1 (Jones et al., 1995), XeFGF (a gift from Dr H. Okamoto), Xwnt11 (Tada and Smith, 2000), Xdsh with Myc-tag (Sokol, 1996), Xdsh-∆DEP and Xdsh∆DIX (Wallingford et al., 2000) have been reported previously. Each construct was linearized, and capped m ...
... residues. Xenopus activin (Thomsen et al., 1990), Xnr1 (Jones et al., 1995), XeFGF (a gift from Dr H. Okamoto), Xwnt11 (Tada and Smith, 2000), Xdsh with Myc-tag (Sokol, 1996), Xdsh-∆DEP and Xdsh∆DIX (Wallingford et al., 2000) have been reported previously. Each construct was linearized, and capped m ...
Crosstalk between Notch signaling Pathway and Glutamine uptake
... In the immune system, Notch signaling is intimately involved in the process of T versus B lymphocyte differentiation from a common lymphocyte progenitor (Radtke, F. 2004[17] ) . Earlier studies have shown that activation of naive CD4+ T cells along with simultaneous activation of Notch1 signaling pat ...
... In the immune system, Notch signaling is intimately involved in the process of T versus B lymphocyte differentiation from a common lymphocyte progenitor (Radtke, F. 2004[17] ) . Earlier studies have shown that activation of naive CD4+ T cells along with simultaneous activation of Notch1 signaling pat ...
RNA Interference of Signal Peptide-binding Protein SRP54 Elicits
... (5). SRP then targets the nascent chain-ribosome complex to the ER membrane by interacting with a membrane-bound SRP receptor. After this docking event that is GTP-dependent, the translocation of the polypeptide takes place co-translationally through the protein pore, the translocon (6, 7). The well ...
... (5). SRP then targets the nascent chain-ribosome complex to the ER membrane by interacting with a membrane-bound SRP receptor. After this docking event that is GTP-dependent, the translocation of the polypeptide takes place co-translationally through the protein pore, the translocon (6, 7). The well ...
Hedgehog signaling pathway
The Hedgehog signaling pathway (or signalling pathway; see spelling differences) is a signaling pathway that transmits information to embryonic cells required for proper development. Different parts of the embryo have different concentrations of hedgehog signaling proteins. The pathway also has roles in the adult. Diseases associated with the malfunction of this pathway include basal cell carcinoma.The Hedgehog signaling pathway is one of the key regulators of animal development and is present in all bilaterians. The pathway takes its name from its polypeptide ligand, an intercellular signaling molecule called Hedgehog (Hh) found in fruit flies of the genus Drosophila. Hh is one of Drosophila's segment polarity gene products, involved in establishing the basis of the fly body plan. The molecule remains important during later stages of embryogenesis and metamorphosis.Mammals have three Hedgehog homologues, DHH, IHH, and SHH, of which Sonic (SHH) is the best studied. The pathway is equally important during vertebrate embryonic development. In knockout mice lacking components of the pathway, the brain, skeleton, musculature, gastrointestinal tract and lungs fail to develop correctly. Recent studies point to the role of Hedgehog signaling in regulating adult stem cells involved in maintenance and regeneration of adult tissues. The pathway has also been implicated in the development of some cancers. Drugs that specifically target Hedgehog signaling to fight this disease are being actively developed by a number of pharmaceutical companies.