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... state. The activation of the BMP pathway within GSCs results in the transcriptional repression of the differentiation factor bag of marbles (bam). The Nanos-Pumilio translational repressor complex and the miRNA pathway also help to promote GSC selfrenewal. How the activities of different transcripti ...
... state. The activation of the BMP pathway within GSCs results in the transcriptional repression of the differentiation factor bag of marbles (bam). The Nanos-Pumilio translational repressor complex and the miRNA pathway also help to promote GSC selfrenewal. How the activities of different transcripti ...
The Formation of the Central Element of the
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
Here. - Blumenstiel Lab
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
Folie 1 - uni
... EGFR signaling and cell cycle control, and to uncover potential regulatory patterns {Uhlmann, 2012 #3}. This unraveled a network of modules being co-regulated by miRNAs. We have available the cell-lysates from the original screen as well as a number of validated antibodies and all required technolog ...
... EGFR signaling and cell cycle control, and to uncover potential regulatory patterns {Uhlmann, 2012 #3}. This unraveled a network of modules being co-regulated by miRNAs. We have available the cell-lysates from the original screen as well as a number of validated antibodies and all required technolog ...
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... mathematical models from these observations, and thereafter test their predictions using genetic methods to alter transcription factor activity and transgenesis to test the effects of modified DNA regulatory elements. These approaches are greatly aided by several advantages of early embryos. First, ...
... mathematical models from these observations, and thereafter test their predictions using genetic methods to alter transcription factor activity and transgenesis to test the effects of modified DNA regulatory elements. These approaches are greatly aided by several advantages of early embryos. First, ...
REVIEWS TBP-associated factors (TAF s): multiple, selective
... Although the whole-genome expression studies have clearly revealed that individual TAFIIs function as promoterselectivity factors, their mechanism of action remains to be elucidated. The three major proposed mechanisms of action are that TAFIIs: (1) serve as activator-binding sites, (2) mediate core ...
... Although the whole-genome expression studies have clearly revealed that individual TAFIIs function as promoterselectivity factors, their mechanism of action remains to be elucidated. The three major proposed mechanisms of action are that TAFIIs: (1) serve as activator-binding sites, (2) mediate core ...
Slide 1 - McGill University
... Structure of Cholecystokinin R eceptor Binding Sites and Mechanism of Activation/Inactivation by Agonists/Antagonists A wide repertoire of physiological effects of CCK and/or gastrin which mediated CCK1 and/or CCK2 receptors has been identified. Among actions of CCK which are mediated by the CCK1R, ...
... Structure of Cholecystokinin R eceptor Binding Sites and Mechanism of Activation/Inactivation by Agonists/Antagonists A wide repertoire of physiological effects of CCK and/or gastrin which mediated CCK1 and/or CCK2 receptors has been identified. Among actions of CCK which are mediated by the CCK1R, ...
Heat Shock Response and Protein Degradation
... synthesis and reduced degradation. The downstream effect of HSF2 activation by proteasome inhibitors is the induction of the same set of heat shock genes that are induced during heat shock by HSF1, thus revealing that HSF2 affords the cell with a novel heat shock gene-regulatory mechanism to respond ...
... synthesis and reduced degradation. The downstream effect of HSF2 activation by proteasome inhibitors is the induction of the same set of heat shock genes that are induced during heat shock by HSF1, thus revealing that HSF2 affords the cell with a novel heat shock gene-regulatory mechanism to respond ...
Complementary Signaling Pathways Regulate the Unfolded Protein
... Summary The unfolded protein response (UPR) is a transcriptional and translational intracellular signaling pathway activated by the accumulation of unfolded proteins in the lumen of the endoplasmic reticulum (ER). We have used C. elegans as a genetic model system to dissect UPR signaling in a multic ...
... Summary The unfolded protein response (UPR) is a transcriptional and translational intracellular signaling pathway activated by the accumulation of unfolded proteins in the lumen of the endoplasmic reticulum (ER). We have used C. elegans as a genetic model system to dissect UPR signaling in a multic ...
Immunity Hemopoiesis and Cellular Drosophila
... possessing a unique ability to achieve somatic gene rearrangement, which enables it to produce a myriad of Abs and receptors. The mammalian innate cellular immune response comes from both the lymphoid and myeloid lineages; among these neutrophils, macrophages, and dendritic cells are professional ph ...
... possessing a unique ability to achieve somatic gene rearrangement, which enables it to produce a myriad of Abs and receptors. The mammalian innate cellular immune response comes from both the lymphoid and myeloid lineages; among these neutrophils, macrophages, and dendritic cells are professional ph ...
2. Summary Final Thesis
... promote survival and proliferation of the cells, e.i FLT3 and c-KIT protein receptors. The second class of mutations impairs transcription factor that are essential for the regulation of normal myeloid development, e.i translocations of the genes targeting myeloid differentiation. Occurrence of clas ...
... promote survival and proliferation of the cells, e.i FLT3 and c-KIT protein receptors. The second class of mutations impairs transcription factor that are essential for the regulation of normal myeloid development, e.i translocations of the genes targeting myeloid differentiation. Occurrence of clas ...
Review Article
... and SWI6 for activation at START, whereas CLBS may their regulation, both CLN2 and CLN3 gene products be regulated by the transcription factor DSCl (see have been shown to interact directly with ~ 3 4 ' ~ to ' ~ ~ below). form a complex in which ~ 3 4 ' ~is' active ~ ~ as a protein Although transcri ...
... and SWI6 for activation at START, whereas CLBS may their regulation, both CLN2 and CLN3 gene products be regulated by the transcription factor DSCl (see have been shown to interact directly with ~ 3 4 ' ~ to ' ~ ~ below). form a complex in which ~ 3 4 ' ~is' active ~ ~ as a protein Although transcri ...
Cytokine receptor signaling through the Jak–Stat–Socs pathway in
... Eight mammalian Socs proteins have been identified: Socs1–7 and cytokine-inducible SH2 protein (Cis). Members of the Socs family of proteins possess three domains: an N-terminal domain of variable length that is not well conserved between members and whose function remains largely unknown; a central ...
... Eight mammalian Socs proteins have been identified: Socs1–7 and cytokine-inducible SH2 protein (Cis). Members of the Socs family of proteins possess three domains: an N-terminal domain of variable length that is not well conserved between members and whose function remains largely unknown; a central ...
Hornstein, E. - Weizmann Institute of Science
... to generate a single strand miRNA. This mature miRNA recruits a protein complex (RISC, greens) that binds to the 3’ untranslated region of messenger RNA (blue) and target it either to cleavage or translational repression. ...
... to generate a single strand miRNA. This mature miRNA recruits a protein complex (RISC, greens) that binds to the 3’ untranslated region of messenger RNA (blue) and target it either to cleavage or translational repression. ...
A Novel Role of Annexin A2 in Human Type I Collagen Gene
... fibroblast cell line WI38, a transcriptional activator binds to the CME and probably functions cooperatively with the adjacent CBE binding factors in the regulation of the COL1A2 gene [Collins et al., 1997]. However, upon WI38 fibroblast transformation with the DNA tumour virus SV40 (termed SV-WI38), ...
... fibroblast cell line WI38, a transcriptional activator binds to the CME and probably functions cooperatively with the adjacent CBE binding factors in the regulation of the COL1A2 gene [Collins et al., 1997]. However, upon WI38 fibroblast transformation with the DNA tumour virus SV40 (termed SV-WI38), ...
PDF
... were present maternally and throughout stages 1–8, including stages where no immunostaining for HRO-WNT-A was detected (Figure 2b; in these experiments, we used Hro-wnt-A oligos that span an intron to avoid problems of genomic contamination, and we amplified 18S rRNA in parallel for each sample to c ...
... were present maternally and throughout stages 1–8, including stages where no immunostaining for HRO-WNT-A was detected (Figure 2b; in these experiments, we used Hro-wnt-A oligos that span an intron to avoid problems of genomic contamination, and we amplified 18S rRNA in parallel for each sample to c ...
CONTRIBUTION OF STEM CELLS AND DIFFERENTIATED CELLS
... choice of lineage being determined by the specific location or microenvironment of the cell3,8 (FIG. 2b). Stem cells are not the only cells within the epidermis that are capable of proliferation. Stem-cell progeny that are destined to undergo terminal differentiation can divide a small number of tim ...
... choice of lineage being determined by the specific location or microenvironment of the cell3,8 (FIG. 2b). Stem cells are not the only cells within the epidermis that are capable of proliferation. Stem-cell progeny that are destined to undergo terminal differentiation can divide a small number of tim ...
TREM2/DAP12 Complex Regulates Inflammatory Responses in
... in cells that participate in innate immune responses. By pairing with different triggering receptors expressed on myeloid cell (TREM) proteins, DAP12 can mediate both positive and negative cellular responses. In particular, TREM1 acts as an amplifier of the immune response, while TREM2 functions as ...
... in cells that participate in innate immune responses. By pairing with different triggering receptors expressed on myeloid cell (TREM) proteins, DAP12 can mediate both positive and negative cellular responses. In particular, TREM1 acts as an amplifier of the immune response, while TREM2 functions as ...
Apoptosis of Lung Epithelial Cells in Response to Meconium and
... Meconium aspiration induces inflammation of the lungs leading to clinical Meconium Aspiration Syndrome (MAS). Clinically MAS manifests hypoxia, hyperoxia, and acidosis requiring oxygen therapy, assisted ventilation, and in severe cases inhaled nitric oxide therapy and Extracorporeal Membrane Oxygena ...
... Meconium aspiration induces inflammation of the lungs leading to clinical Meconium Aspiration Syndrome (MAS). Clinically MAS manifests hypoxia, hyperoxia, and acidosis requiring oxygen therapy, assisted ventilation, and in severe cases inhaled nitric oxide therapy and Extracorporeal Membrane Oxygena ...
Reference
... dephosphorylation of c-Jun at the C-terminal domain (8), the functional role of this dephosphorylation in regulating the c-Jun/Sp1 interaction was investigated. We found that PMA induced dephosphorylation of c-Jun C-terminus in A431 cells. c-Jun mutant TAM-67-M3A, which contains three substitute ala ...
... dephosphorylation of c-Jun at the C-terminal domain (8), the functional role of this dephosphorylation in regulating the c-Jun/Sp1 interaction was investigated. We found that PMA induced dephosphorylation of c-Jun C-terminus in A431 cells. c-Jun mutant TAM-67-M3A, which contains three substitute ala ...
Differential roles for the low-affinity phosphate transporters Pho87
... as the pho84Δ strain is unable to rapidly reverse Pi -starvation responses. However, we previously observed rapid Pi signalling in strains deleted of PHO4 or PHO81 [19], both of which do not display expression of PHO84. Thus either expression of PHO84 below detection levels is sufficient to sustain ...
... as the pho84Δ strain is unable to rapidly reverse Pi -starvation responses. However, we previously observed rapid Pi signalling in strains deleted of PHO4 or PHO81 [19], both of which do not display expression of PHO84. Thus either expression of PHO84 below detection levels is sufficient to sustain ...
The phosphatidylinositol transfer protein RdgBβ binds 14-3
... were trypsinized, washed and re-suspended in 240 μl of SET buffer [0.25 M sucrose, 1 mM EDTA and 10 mM Tris/HCl (pH 7.4)] with protease inhibitor cocktail (Sigma–Aldrich). To harvest the cytosol, the cells were sonicated 3×15 s followed by ultracentrifugation at 43 000 rev./min for 60 min at 4 ◦ C ( ...
... were trypsinized, washed and re-suspended in 240 μl of SET buffer [0.25 M sucrose, 1 mM EDTA and 10 mM Tris/HCl (pH 7.4)] with protease inhibitor cocktail (Sigma–Aldrich). To harvest the cytosol, the cells were sonicated 3×15 s followed by ultracentrifugation at 43 000 rev./min for 60 min at 4 ◦ C ( ...
Provisional PDF - BioMed Central
... as Nova1, RBFox1 and nPTB, and their targets, have been identified in neural tissues and cells [5-7]. Many isoforms produced by alternative splicing have distinctly different functions, and play important biological roles [8,9]. Therefore, it is important to determine critical alternative splicing n ...
... as Nova1, RBFox1 and nPTB, and their targets, have been identified in neural tissues and cells [5-7]. Many isoforms produced by alternative splicing have distinctly different functions, and play important biological roles [8,9]. Therefore, it is important to determine critical alternative splicing n ...
MODERN METHODS in BIOCHEMISTRY
... be connected to ligands or spacers which contain primary amine groups (such as proteins). • Cyanogen bromide is extremely toxic as it releases hydrogen cyanide on acidification. ...
... be connected to ligands or spacers which contain primary amine groups (such as proteins). • Cyanogen bromide is extremely toxic as it releases hydrogen cyanide on acidification. ...
Hedgehog signaling pathway
The Hedgehog signaling pathway (or signalling pathway; see spelling differences) is a signaling pathway that transmits information to embryonic cells required for proper development. Different parts of the embryo have different concentrations of hedgehog signaling proteins. The pathway also has roles in the adult. Diseases associated with the malfunction of this pathway include basal cell carcinoma.The Hedgehog signaling pathway is one of the key regulators of animal development and is present in all bilaterians. The pathway takes its name from its polypeptide ligand, an intercellular signaling molecule called Hedgehog (Hh) found in fruit flies of the genus Drosophila. Hh is one of Drosophila's segment polarity gene products, involved in establishing the basis of the fly body plan. The molecule remains important during later stages of embryogenesis and metamorphosis.Mammals have three Hedgehog homologues, DHH, IHH, and SHH, of which Sonic (SHH) is the best studied. The pathway is equally important during vertebrate embryonic development. In knockout mice lacking components of the pathway, the brain, skeleton, musculature, gastrointestinal tract and lungs fail to develop correctly. Recent studies point to the role of Hedgehog signaling in regulating adult stem cells involved in maintenance and regeneration of adult tissues. The pathway has also been implicated in the development of some cancers. Drugs that specifically target Hedgehog signaling to fight this disease are being actively developed by a number of pharmaceutical companies.