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Plant Nutrition: Root Transporters on the Move
Plant Nutrition: Root Transporters on the Move

... retromer complex (for review, see Seaman, 2012). In mammals, the retromer is composed of two subcomplexes: the core retromer constituted by Vacuolar Protein Sorting (VPS)26, VPS29, and VPS35 and a dimer of Sorting Nexin (SNX; Seaman, 2012). In Arabidopsis, all members of the retromer are conserved; ...
Georgatsou E. and Alexandraki D. - IMBB-FORTH in
Georgatsou E. and Alexandraki D. - IMBB-FORTH in

... Since BPS is a chelator of both metals and induction in its presence cannot be attributed to either iron or copper depletion alone, we have additionally examined RNA accumulation in cells grown in YNBFC medium (SD medium without iron and copper), adding either iron or copper at standard concentratio ...
Homework # 7 Amino Acids, Proteins, and Enzymes
Homework # 7 Amino Acids, Proteins, and Enzymes

... molecular structure capable of recognizing a complementary molecular structure on the antigen which might be some proteins, polysaccharides, and nucleic acids. Small organic foreign molecules do not by themselves elicit antibody formation unless they become bonded to one of the larger biomolecules l ...
Cloning and characterization of the
Cloning and characterization of the

... coli synthase TruA (6). scPus1p and mPus1p have similar substrate specificities while the target RNAs of scPus2p have not yet been determined. Surprisingly, scPus1p also converts uridines at position 44 in U2 small nuclear (sn)RNA and is therefore the first yeast pseudouridine synthase characterized ...
PURIFICATION OF TAP TAGGED YEAST PROTEINS  Annika Väntänen
PURIFICATION OF TAP TAGGED YEAST PROTEINS Annika Väntänen

... long-chain fatty acids. These mitochondrially synthesised long-chain fatty acids, for example 3-hydroxymyristoyl-ACP, are possibly used for protein acylation which is one type of lipid modifications of proteins. The best characterised physiological function of the pathway is to provide the octanoyl ...
ABC Pumps and Their Role in Active Drug Transport
ABC Pumps and Their Role in Active Drug Transport

... Keywords: Blood brain barrier, ABC transporters, P-glycoprotein, MRPs, drug absorption and disposition. INTRODUCTION The therapeutic effect of many drugs is due to their ability to cross cellular barriers for targeting the biological sites. Usually it is accepted that lipophilic drugs can cross thes ...
AtVPS45 Is a Positive Regulator of the SYP41
AtVPS45 Is a Positive Regulator of the SYP41

... dwarfed. The severity of the dwarf phenotype correlated well with the amount of AtVPS45 protein detected by immunoblot (Fig. 1B), suggesting that AtVPS45 is required for normal plant growth. Although the RNAi construct does not target other SM genes, we nevertheless checked the levels of the SM prot ...
Protein Structure - Oregon State University
Protein Structure - Oregon State University

... You're lovely and serene
 With twenty zwitterions like
 Cysteine and alanine 
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Protein Structure Prediction
Protein Structure Prediction

... • Large numbers of genes being sequenced - need structures • Protein folding (from primary sequence to tertiary structure) is a fast self-organising process where a disordered non-functional chain of amino acids becomes a stable, compact, and functional molecule • The free energy difference between ...
kaedah-kaedah dalam biologi molekul
kaedah-kaedah dalam biologi molekul

... CENTRIFUGATION • PARTICLES IN SAMPLE WILL SEPARATE INTO SUPERNATANT AND PELLET OR IN BOTH DEPENDING ON THEIR SIZE, SHAPE, DENSITY AND CENTRIFUGATION CONDITION • THE PELLET CONTAINS ALL THE SEDIMENTED COMPONENT MIXTURE AND CAN CONTAIN MATERIALS THAT WAS NOT ...
Advances in Amino Acid Analysis
Advances in Amino Acid Analysis

... search branched into two broad paths: One following DNA and leading to modern molecular biology, and the other focusing on characterizing and understanding the function of proteins, the products of the genome. Genomic and protein sciences diverged into separate disciplines but were always linked by ...
Hongzhi Li School of Life Science
Hongzhi Li School of Life Science

... Membrane compartmentalization allows specialized activities to proceed without external interference and enables cellular activities to be regulated independently of one another. ...
Perspective
Perspective

... Two-membrane prokaryotic cells are divided into at least five compartments: the cytoplasm, the inner membrane, the periplasm between the two membranes, the outer membrane, and the extracellular milieu. Moreover, the inner and outer leaflets of both membrane bilayers can be considered to be distinct ...
Different subcellular locations of secretome components of
Different subcellular locations of secretome components of

... to the existence of a smaller number of cell wall components in this part of the cell, it might be more suited for the accumulation of large proteins. ActA of the Gram-positive intracellular bacterial pathogen Listeria monocytogenes also spans the bacterial membrane and the peptidoglycan, thereby ex ...
Optimization of Ammonium Sulfate Concentration for Purification of
Optimization of Ammonium Sulfate Concentration for Purification of

... has previously been fused with the human immunoglobulin Fc fragment carrying the ER retention sequence, to produce the recombinant antigen-antibody complex GA733-FcK in a plant expression system (Lu et al., 2012; Park et al., 2015a,b). Moreover, expression of the recombinant protein GA733-FcK has be ...
Introduction to Proteins II
Introduction to Proteins II

... The Sequence of Amino Acids in a Protein [S23] a. The exact sequence of amino acids is unique for each protein. Protein Structure [S24] a. Is all the information necessary for a protein to fold just based on that primary sequence? The native structure in how a protein folds – is it based completely ...
How Much Protein? - Hammer Nutrition
How Much Protein? - Hammer Nutrition

... (BCOAD). This enzyme is relatively low at rest (4-7%), but during exercise, dephosphorylation releases it to as high as +25%. After exercise, both BCOAD and Leucine oxidation are attenuated, though total BCOAD is up-regulated.[2] Some research utilize higher amino acid catabolism, (+15%) during endu ...
What is an Excellent Source of Protein? Protein Quality
What is an Excellent Source of Protein? Protein Quality

... • Take Home Messages ...
Tomato LeAGP-1 is a plasma membrane-bound
Tomato LeAGP-1 is a plasma membrane-bound

... C-terminal sequence identified as a putative GPI-anchor addition sequence (Pogson and Davies 1995, Li and Showalter 1996). The pattern of LeAGP-1 expression in cultured cells and plants on the mRNA level as well as the protein level is known, the later analysis being facilitated with a LeAGP-1 speci ...
Structure - chula ise
Structure - chula ise

... Function: generate energy in the form of ATP in eucaryotes. Most of a eukaryotic cell’s ATP is generated from oxidation reactions (fatty acid breakdown, Kreb’s cycle) in the mitocondrion using a proton gradient set up in the space between the two membranes (chemiosmotic coupling). ...
Cancer Prone Disease Section Oculocutaneous Albinism Atlas of Genetics and Cytogenetics
Cancer Prone Disease Section Oculocutaneous Albinism Atlas of Genetics and Cytogenetics

... NP_000363.1) composed of 529 amino acids. TYR is a melanosomal membrane bound glycoenzyme with a type-3 copper active site. The mature TYR polypeptide includes an 18-amino acid long N-terminal signal peptide, six Nglycosylation sites, two copper binding sites (CuA and CuB) and one transmembrane (TM) ...
- Wiley Online Library
- Wiley Online Library

... eliminate the ability of the ribosome to start translation from this AUG1, but no expression of FH is expected from this first AUG1 due to the downstream frame shift. This construct allows us to test if the translation from the second AUG is possible in the presence of an intact first AUG. As shown ...
Full Text
Full Text

... sure specific characteristics of a biological system (e.g., the sequence of a gene or its level of expression) in a high-throughput manner, opening the possibility of automated functional identification. Automated functional identification is based on the principle of functional similarity, such tha ...
Diversity and Formation of Endoplasmic Reticulum
Diversity and Formation of Endoplasmic Reticulum

... et al., 2001). All the compartments that have been characterized share three common features: (1) The ER-derived compartments accumulate a large amount of a single protein or only a few different proteins. (2) The protein components do not act within the compartments, even if they are functional pro ...
Cell Transport - HRSBSTAFF Home Page
Cell Transport - HRSBSTAFF Home Page

... Membrane is permeable to water, but impermeable to larger molecules ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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