Rapid Translation System RTS 500 E. coli HY Kit
... Cell-free protein expression has a strong potential to meet these requirements because handling of intact cells (e.g., transformation, fermentation, lysis) is not necessary. In fact, this methodology only involves liquid handling and can thus be adapted to automated processes. Low yield has been the ...
... Cell-free protein expression has a strong potential to meet these requirements because handling of intact cells (e.g., transformation, fermentation, lysis) is not necessary. In fact, this methodology only involves liquid handling and can thus be adapted to automated processes. Low yield has been the ...
The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can
... proteins is consistent with that of a prevacuolar compartment (7). As can be seen, Gef1-GFP (but not Nhx1) is also present in Golgi fractions, consistent with previous studies (9, 23). An A. thaliana Homologue of NHX1 Functions in Yeast. The yeast strain we have built provides an important tool for ...
... proteins is consistent with that of a prevacuolar compartment (7). As can be seen, Gef1-GFP (but not Nhx1) is also present in Golgi fractions, consistent with previous studies (9, 23). An A. thaliana Homologue of NHX1 Functions in Yeast. The yeast strain we have built provides an important tool for ...
Novel Food Information - Drought Tolerant Corn - MON 87460
... collected for expression in all the previously assessed tissues under wellwatered and waterlimited conditions. Results from this trial were presented on a freshweight (fwt) and dryweight basis (dwt). Protein expression levels, both the mean and range values, were shown to be comparable between t ...
... collected for expression in all the previously assessed tissues under wellwatered and waterlimited conditions. Results from this trial were presented on a freshweight (fwt) and dryweight basis (dwt). Protein expression levels, both the mean and range values, were shown to be comparable between t ...
Full Text - Biochemical Society Transactions
... transcripts are detected in root tissues [20]. Our findings (summarized in Figure 1) reveal an unexpected degree of tissue specificity for different TIP isoforms. For example, a YFP fusion with TIP1;2, whose transcript is abundant and distributed throughout the root axis [19], is only detected at th ...
... transcripts are detected in root tissues [20]. Our findings (summarized in Figure 1) reveal an unexpected degree of tissue specificity for different TIP isoforms. For example, a YFP fusion with TIP1;2, whose transcript is abundant and distributed throughout the root axis [19], is only detected at th ...
GMS 6062 Pro Traf 02
... Mironov, A. A., Beznoussenko, G. V., Nicoziani, P., Martella, O., Trucco, A., Kweon, H. S., Di Giandomenico, D., Polishchuk, R. S., Fusella, A., Lupetti, P., et al. 2001. Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae, J ...
... Mironov, A. A., Beznoussenko, G. V., Nicoziani, P., Martella, O., Trucco, A., Kweon, H. S., Di Giandomenico, D., Polishchuk, R. S., Fusella, A., Lupetti, P., et al. 2001. Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae, J ...
De Novo Mutation Found in the Porphobilinogen Deaminase Gene
... of PBGD by reverse transcription and amplified a fragment containing the complete encoding region. This fragment was inserted into prokaryotic expression vector. The cells of recombinant strain E. coli BL21 were transformed by this construct. By side-directed mutagenesis we introduced desired mutati ...
... of PBGD by reverse transcription and amplified a fragment containing the complete encoding region. This fragment was inserted into prokaryotic expression vector. The cells of recombinant strain E. coli BL21 were transformed by this construct. By side-directed mutagenesis we introduced desired mutati ...
Chapter 5 Proteins - Liberty Public Schools
... functions; methionine begins protein synthesis, proline causes kinks in the protein polymer, ...
... functions; methionine begins protein synthesis, proline causes kinks in the protein polymer, ...
The role of protein–protein interactions in the intracellular traffic of
... different isoforms of 14-3-3 (σ, β, θ and ζ) were found to interact with TASK-1 [88]. Screening of a human heart cDNA library with the last 16 amino acids of human TASK-1 independently yielded 14-3-3β as an interacting protein [76]. Progressive truncation of the C-terminus from the proximal side sho ...
... different isoforms of 14-3-3 (σ, β, θ and ζ) were found to interact with TASK-1 [88]. Screening of a human heart cDNA library with the last 16 amino acids of human TASK-1 independently yielded 14-3-3β as an interacting protein [76]. Progressive truncation of the C-terminus from the proximal side sho ...
Choosing between pathogenicity and saprophytism: A case study
... genes and other extraneous sequences of phage genomic origin were removed by ‘webtraceminer’ (http://www.coniferdb.org/software/wtm1.2/), using the NCBI UniVec database as reference. ESTs with long poly(A) tails and those with more than 3% ambiguous base reads were also eliminated, generating a tota ...
... genes and other extraneous sequences of phage genomic origin were removed by ‘webtraceminer’ (http://www.coniferdb.org/software/wtm1.2/), using the NCBI UniVec database as reference. ESTs with long poly(A) tails and those with more than 3% ambiguous base reads were also eliminated, generating a tota ...
Journal club
... • Screened a number of E. coli promoters to work upstream of cel3A. • One of these, wrbA, gives growth rates nearly the same on cellobiose as on glucose (and there is no added cellobiose transporter, so must be using a glucose transporter OK – he never considers transport!) • Did the same with the x ...
... • Screened a number of E. coli promoters to work upstream of cel3A. • One of these, wrbA, gives growth rates nearly the same on cellobiose as on glucose (and there is no added cellobiose transporter, so must be using a glucose transporter OK – he never considers transport!) • Did the same with the x ...
3040 - Zhang, Hongyu
... target protein based on the template structure. The major steps in building the homology model are the conserved region modelling and loop region modelling. Conserved regions refer to those regions with conserved amino acids in the sequence alignment, most often those regions having standard seconda ...
... target protein based on the template structure. The major steps in building the homology model are the conserved region modelling and loop region modelling. Conserved regions refer to those regions with conserved amino acids in the sequence alignment, most often those regions having standard seconda ...
osmosis - Biofizika
... Transmembrane proteins – It can bind to the hydrophobic part of the membrane. Peripheral membrane proteins– not directly linked to the membrane. Glycoproteins - these oligosaccharides are attached to the extracellular side of the membrane proteins. Glycosyl-phosphatidylinositol (GPI) - are covalentl ...
... Transmembrane proteins – It can bind to the hydrophobic part of the membrane. Peripheral membrane proteins– not directly linked to the membrane. Glycoproteins - these oligosaccharides are attached to the extracellular side of the membrane proteins. Glycosyl-phosphatidylinositol (GPI) - are covalentl ...
structure and function of procaryotic cells
... material that has high tensile strength. Such a material is murein, the ubiquitous component of bacterial cell walls. The cell walls of Bacteria deserve special attention for several reasons: 1. They are an essential structure for viability, as described above. 2. They are composed of unique compone ...
... material that has high tensile strength. Such a material is murein, the ubiquitous component of bacterial cell walls. The cell walls of Bacteria deserve special attention for several reasons: 1. They are an essential structure for viability, as described above. 2. They are composed of unique compone ...
Proteins
... D. Amino acids with basic side chains The side chain radicals of basic amino acids accept protons (see Figure 1.3). At physiologic pH the side radicals in lysine and arginine residues are positively charged. In contrast, histidine is slightly basic. Therefore, it is mainly uncharged at physiologic p ...
... D. Amino acids with basic side chains The side chain radicals of basic amino acids accept protons (see Figure 1.3). At physiologic pH the side radicals in lysine and arginine residues are positively charged. In contrast, histidine is slightly basic. Therefore, it is mainly uncharged at physiologic p ...
Chapter 27 Protein Metabolism
... There are about 25 of them in an E. coli cell. There are two major subunits, each with multiple proteins. They are relatively small, with molecular weights less than 10,000. ...
... There are about 25 of them in an E. coli cell. There are two major subunits, each with multiple proteins. They are relatively small, with molecular weights less than 10,000. ...
PDF
... shown that phactr3, also known as scapinin, localizes to the plasma membrane, including lamellipodia and membrane ruffles. In the present study, experiments using deletion and point mutants showed that the basic and hydrophobic residues in the N-terminus play crucial roles in the localization to the ...
... shown that phactr3, also known as scapinin, localizes to the plasma membrane, including lamellipodia and membrane ruffles. In the present study, experiments using deletion and point mutants showed that the basic and hydrophobic residues in the N-terminus play crucial roles in the localization to the ...
novel nucleotide carrier proteins of Protochlamydia
... et al., 1999; Linka et al., 2003; Daugherty et al., 2004). PamNTT2 transports all four RNA nucleotides, and most remarkably, PamNTT4 has been identified as the first carrier protein able to transport nicotineamide adenine dinucleotide (NAD+) in an intact form across a biological membrane (Haferkamp ...
... et al., 1999; Linka et al., 2003; Daugherty et al., 2004). PamNTT2 transports all four RNA nucleotides, and most remarkably, PamNTT4 has been identified as the first carrier protein able to transport nicotineamide adenine dinucleotide (NAD+) in an intact form across a biological membrane (Haferkamp ...
Amino acids introduction
... Amino acids form chains, the sequence or primary structure. These chains fold in -helices, b-strands, b-turns, and loops (or for short, helix, strand, turn and loop), the secondary structure. These secondary structure elements fold further to make whole proteins, but more about that later. There ar ...
... Amino acids form chains, the sequence or primary structure. These chains fold in -helices, b-strands, b-turns, and loops (or for short, helix, strand, turn and loop), the secondary structure. These secondary structure elements fold further to make whole proteins, but more about that later. There ar ...
2012 patel DE perox
... are shown in Fig. 8. As the activity observed in a cell lysate depends on both the intrinsic activity of a protein and the level of its expression, we estimated expression levels by gel electrophoresis. As shown in Fig. 9, all sequences expressed at similar levels. ...
... are shown in Fig. 8. As the activity observed in a cell lysate depends on both the intrinsic activity of a protein and the level of its expression, we estimated expression levels by gel electrophoresis. As shown in Fig. 9, all sequences expressed at similar levels. ...