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elucidate the contribution of proteins to tears. a challenge for
elucidate the contribution of proteins to tears. a challenge for

... indicates that pure solutions of lysozyme, lactoferrin, IgA, or tears without lipids behave as Newtonian fluids i.e. as an object is moved faster through a fluid (shear rate) the resistance it incurs (viscosity) does not change. Whole tears (with lipids) behave quite differently: their viscosity dro ...
Molecular properties of cardiac tail
Molecular properties of cardiac tail

... membrane protein SLMAP are consistent with structural role in arrangement of excitation-contraction coupling apparatus. Am J Physiol Heart Circ Physiol 288: H1810 –H1819, 2005. First published December 9, 2004; doi:10.1152/ajpheart.01015.2004.—The spatial arrangement of the cell-surface membranes (s ...
Protein thermostability in Archaea and Eubacteria
Protein thermostability in Archaea and Eubacteria

... Phe, Tyr, Asn, Lys, Gln, Thr, His, Ser, and Asn, in thermophiles but not in mesophiles (Kreil and Ouzounis, 2001). The authors attribute the observed unexpected higher values of Met and Val to the fact that Met is the start codon for almost all proteins, and that Met and Val belong to a conserved gr ...
Purification and proteomic characterization of plastids from Brassica
Purification and proteomic characterization of plastids from Brassica

... greater than 95% of the cell area on TEM micrographs [10]. 3.2 Embryoplastid isolation from 3 WAF embryos Previously published methods [5, 6] for embryoplast isolation from rapeseed rely on mechanical disruption of tissue in osmotically balanced media to release intact plastids. Our initial efforts ...
Operon Models
Operon Models

... 1. Using a serrated knife, cut an 8-inch segment from the noodle. This will be used as the repressor protein. 2. Again, each end of the noodle/operon should feature an unlabeled/untaped section, to show the continuation of the DNA strand. 3. Wrap spirals of colored electrical tape (or shade the nood ...
Transport Between the Endoplasmic Reticulum and Golgi Apparatus
Transport Between the Endoplasmic Reticulum and Golgi Apparatus

... Anterograde Transport from the ER is Mediated by COPII Although COPII-mediated export of proteins from the ER is not the only anterograde ER–Golgi route that has been proposed in plants, it has received the most attention and is therefore the best-characterized pathway between these organelles at th ...
Which Protein is Best?
Which Protein is Best?

Lecture 6 Thurs 4-13-06
Lecture 6 Thurs 4-13-06

... (MVB = late endosome), and the lysosome. Non-ubiquitinated receptors are recycled to the plasma membrane via recycling endosomes. Mono-ubiquitinated internalized proteins interact with endocytic adapter complexes through surface patches surrounding critical residues within ubiquitin. Adaptor protein ...
sorghum protein - Simply Sorghum
sorghum protein - Simply Sorghum

... bran is a rich source of condensed tannins, also known as proanthocyanidins, specifically containing epicatechin and catechin. Black sorghum bran contains anthocyanins, including 3-deoxyanthocyanins (apigeninidin) and luteolinidin7. The high levels of 3-deoxyanthocyanins are relatively rare in natur ...
The 14-3-3 proteins in regulation of cellular metabolism - BORA
The 14-3-3 proteins in regulation of cellular metabolism - BORA

... and interactomic studies clearly illustrate the diverse biological functions associated with this protein family. The extensive interactome of the 14-3-3 proteins and its regulation by protein phosphorylation events suggest a fundamental function of these proteins in signaling related to cellular me ...
Supplementary Data
Supplementary Data

... Figure S6: The R1 domain: Top) Sequence alignment deduced from the HCA comparison of the N-terminal part of the CFTR R domain (here named the R1 domain) with the regulatory domains found in some bacterial ABC transporters immediately after their NBDs (TOBE domains). The sequences of six of these pr ...
Notes - Part 1.
Notes - Part 1.

... (with negative  values) labelled  and /p correspond to the conformations of the amino acid residues in the common secondary structures. R corresponds to the helix (also known as the 3.613 helix), This has 3.6 residues per turn. It is a righthanded helix, with the carbonyls pointing towards the ...
Barbara Soldo
Barbara Soldo

... of interdomain transfer of aminoacyl to TycA holo-PCP domain added in trans. TycA PCP domain was coexpressed together with Srf protein, catalyzing its in vivo posttranslational priming with 4’-phosphopantetein cofactor. Although, HPLC analysis (not shown) indicates that the majority of PCP protein i ...
Effect of Structural Changes in Proteins Derived from GATA4
Effect of Structural Changes in Proteins Derived from GATA4

Influence of genomic G+ C content on average amino
Influence of genomic G+ C content on average amino

... (-5.4%), and Gly (+5.3%) in IMP, and for Ala (+9.3%), Lys ( - 8.6%), Asn (-6.2%), Arg (+6.0%) and Ile (-5.8%) in non-IMP. For these amino acids, the absolute variation of their frequencies is similar to their average content in proteins so that their relative variation is close to 100%. Since most p ...
The protein folds as complex natural forms: Evidence that the
The protein folds as complex natural forms: Evidence that the

Releasable conjugation of polymers to proteins
Releasable conjugation of polymers to proteins

... permit the selective modification of all solvent exposed amino acid residues of a given sort. There currently exist residue-specific reactions for permanently coupling polymers to at least 10 out of the 20 canonical amino acids found in proteins.7-10 However, in many cases, polymer-modification can ...
The cytoplasm of living cells: a functional mixture of thousands of
The cytoplasm of living cells: a functional mixture of thousands of

... phase behaviour of models of the cytoplasm, even in the absence of hard data on even one of the millions of interactions that occur in the cytoplasm. In the remainder of this introduction we will consider some of the basic questions we can ask about the cytoplasm. What is in it? A concentrated solut ...
Chapter 5 - macromolecules
Chapter 5 - macromolecules

... • Nucleotide polymers are linked together to build a polynucleotide • Adjacent nucleotides are joined by covalent bonds that form between the —OH group on the 3 carbon of one nucleotide and the phosphate on the 5 carbon on the next abc…lmnop • sugar-phosphate backbone (side of ladder) with nitroge ...
Flow Cytometry Protein A and Protein G Antibody Binding Beads
Flow Cytometry Protein A and Protein G Antibody Binding Beads

Protein primary structure: Amino acids
Protein primary structure: Amino acids

... I. Diversity of proteins Numerous biological functions are performed by proteins. These include oxygen transport and storage (hemoglobin and myoglobin, respectively), muscle functioning (titin), growth of bones (collagen), cell adhesion (fibronectin) etc. The diversity in functions suggests a wide d ...
Chapter 3 - Coastal Bend College
Chapter 3 - Coastal Bend College

... V. Membrane Proteins (MP) • Many fxns of the plasma membrane are determined by the combination of membrane proteins present. • The ability of these proteins to fxn properly is determined by their 3-D shape • There are 2 major types: ...
Anne Ye - A Critical Review of Computational Protein Design Strategies: Progress, Limitations, and Improvements
Anne Ye - A Critical Review of Computational Protein Design Strategies: Progress, Limitations, and Improvements

... catalysis, binding of other proteins or small molecules, and assembly into multimeric structures. Examples of particular interest are noted here. In 2008, a pioneering success in de novo enzyme design was achieved when Rosetta was used to generate the first ever retro-aldolase [21], for which there ...
Degradation signals within both terminal domains of the cauliflower
Degradation signals within both terminal domains of the cauliflower

... (a) The UPR technique. Reference and test proteins are separated by a ubiquitin moiety. This fusion is cleaved by ubiquitin (Ub)-speci®c proteases at the Ub±test protein junction, yielding equimolar amounts of both proteins (Levy et al., 1996). For this study the system was adapted by using two stab ...
The exocyst – the tethering complex for localized exocytosis
The exocyst – the tethering complex for localized exocytosis

... are driven by polar distribution of IAA influx and efflux carriers within the cell plasma membrane (recently reviewed in Friml 2003). Both exocytosis and endocytosis participate in carriers recycling (Geldner et al. 2003). Likewise, cloning of mutant loci of knolle (syntaxin) and gnom (ARF GEF) embr ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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