Purification and Biochemical Characterization of Digestive Lipase in
... Some biochemical characteristics are similar to those found in mammalian digestive lipases: molecular masses of ∼40–50 kDa with an alkaline pI between 6.8 and 7.8 (Moreau et al. 1992; Miled et al. 2000). In crustaceans, lipases are liberated by B cells of the midgut gland (Loizzi and Peterson 1971) ...
... Some biochemical characteristics are similar to those found in mammalian digestive lipases: molecular masses of ∼40–50 kDa with an alkaline pI between 6.8 and 7.8 (Moreau et al. 1992; Miled et al. 2000). In crustaceans, lipases are liberated by B cells of the midgut gland (Loizzi and Peterson 1971) ...
9. Wakil, S. J., Green, DE, Mii, S., and Mahler, HR (1954) Studies on
... 6. Acetyl-CoA carboxylase 2 mutant mice are protected against obesity and diabetes induced by high-fat/high carbohydrate diets. Fatty acid oxidation rates in the soleus muscle and in hepatocytes of Acc2-/- mice were significantly higher than those of WT cohorts and were not affected by the addition ...
... 6. Acetyl-CoA carboxylase 2 mutant mice are protected against obesity and diabetes induced by high-fat/high carbohydrate diets. Fatty acid oxidation rates in the soleus muscle and in hepatocytes of Acc2-/- mice were significantly higher than those of WT cohorts and were not affected by the addition ...
Aspects of Lipid Metabolism in Crustaceans Department of
... acids, whereas the lipid of fresh water forms characteristically yields a relatively larger quantity of saturated C I 6 and CIS fatty acids. Table 2 reveals that the marine crustaceans, P. borealis and M. norvegica, do indeed contain significant quantities of highly unsaturated (polyenic) fatty acid ...
... acids, whereas the lipid of fresh water forms characteristically yields a relatively larger quantity of saturated C I 6 and CIS fatty acids. Table 2 reveals that the marine crustaceans, P. borealis and M. norvegica, do indeed contain significant quantities of highly unsaturated (polyenic) fatty acid ...
Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... to an uronic acid. GAGs reside both in the ECM (extracellular matrix) and at the cell surface as constituents of proteoglycans. These sugars, apart from having important structural roles in the ECM, are also fundamental modulators of many biological processes, such as development, cell proliferation ...
... to an uronic acid. GAGs reside both in the ECM (extracellular matrix) and at the cell surface as constituents of proteoglycans. These sugars, apart from having important structural roles in the ECM, are also fundamental modulators of many biological processes, such as development, cell proliferation ...
Biochemical and genetic analysis of leucine-, isoleucine
... ments, Though their experimental system was limited, from the results of their studies they hypothesized that genes control phenotypic properties by governing the formation of the enzymes of the metabolic pathways of eye pigments. They proposed that each of the constituents of the cell is synthesize ...
... ments, Though their experimental system was limited, from the results of their studies they hypothesized that genes control phenotypic properties by governing the formation of the enzymes of the metabolic pathways of eye pigments. They proposed that each of the constituents of the cell is synthesize ...
The Complete Oxidation of Palmitate Yields 106 Molecules of ATP
... mitochondria into the blood and are transported to peripheral tissues. These ketone bodies were initially regarded as degradation products of little physiological value. However, the results of studies by George Cahill and others revealed that these derivatives of acetyl CoA are important molecules ...
... mitochondria into the blood and are transported to peripheral tissues. These ketone bodies were initially regarded as degradation products of little physiological value. However, the results of studies by George Cahill and others revealed that these derivatives of acetyl CoA are important molecules ...
Journal of Bacteriology
... M MnCl, and 0.02 M ATP, final pH 8.5. added instead of 20 uliters of water. Solvents. The following solvents were used: A, L-Alanine:D-alanine racemase was determined in isobutyric acid-1 M ammonia (5:3, v/v); B, ethanol-1 a mixture containing 10 Aliters of each of the folM ammonium acetate, pH 7.2 ...
... M MnCl, and 0.02 M ATP, final pH 8.5. added instead of 20 uliters of water. Solvents. The following solvents were used: A, L-Alanine:D-alanine racemase was determined in isobutyric acid-1 M ammonia (5:3, v/v); B, ethanol-1 a mixture containing 10 Aliters of each of the folM ammonium acetate, pH 7.2 ...
Purification and Some: Characteristics of a Monomeric Racemase
... the second base of the two-base model proposed by Adams (16), with the Lys 39 residue of the other subunit being the first base. Both Tyr 265 and Lys 39 are conserved in all alanine racemases whose amino acid sequences are known (13). Given that there are very few reports on monomeric alanine racema ...
... the second base of the two-base model proposed by Adams (16), with the Lys 39 residue of the other subunit being the first base. Both Tyr 265 and Lys 39 are conserved in all alanine racemases whose amino acid sequences are known (13). Given that there are very few reports on monomeric alanine racema ...
A modular approach to sphingolipid analogs mediated by aziridines: Synthesis
... galactosylceramides, or higher glycosilated ceramide species called glycosphingolipids. When galactosylceramide adds a sulphate group at 3‐position of the sugar residue gives rise to sulfatides, while the addition of a sialic acid in the carbohydrate head group of glycosphingolipids ...
... galactosylceramides, or higher glycosilated ceramide species called glycosphingolipids. When galactosylceramide adds a sulphate group at 3‐position of the sugar residue gives rise to sulfatides, while the addition of a sialic acid in the carbohydrate head group of glycosphingolipids ...
Conversion of trypsin to a functional threonine protease
... several Tn variants were constructed in which the disulfide bond was removed and Ser 195 was substituted with threonine. The C42A/C58V/S195T-Tn variant (AVT-Tn) was designed to remove the Cys 42–Cys 58 disulfide bond and introduce a hydrophobic character into the modified region. This change was des ...
... several Tn variants were constructed in which the disulfide bond was removed and Ser 195 was substituted with threonine. The C42A/C58V/S195T-Tn variant (AVT-Tn) was designed to remove the Cys 42–Cys 58 disulfide bond and introduce a hydrophobic character into the modified region. This change was des ...
NH2
... •In the liver:The formation of glutamine can be considered as a mechanism for scavenging NH3 that has not been incorporated into urea. •In the brain: It removes the toxic effect of NH3 in the brain .Then the glutamine goes via the blood to the kidneys where it become hydrolyzed by glutaminase into g ...
... •In the liver:The formation of glutamine can be considered as a mechanism for scavenging NH3 that has not been incorporated into urea. •In the brain: It removes the toxic effect of NH3 in the brain .Then the glutamine goes via the blood to the kidneys where it become hydrolyzed by glutaminase into g ...
Saccharomyces cerevisiae Metabolic engineering of for production
... select for better-performing mutants (Vinci & Byng, 1999)]. While classical strain improvement continues to be of great importance in industrial biotechnology, it is increasingly being complemented by metabolic engineering, which has been defined as ‘the improvement of cellular activities by manipul ...
... select for better-performing mutants (Vinci & Byng, 1999)]. While classical strain improvement continues to be of great importance in industrial biotechnology, it is increasingly being complemented by metabolic engineering, which has been defined as ‘the improvement of cellular activities by manipul ...
09_chapter 2
... structures: that is Amygdala and Hippocampus, where processing of emotions and memory occurs respectively. During stress the blood brain barrier also becomes more permeable for corticosteroids, resulting in the activation of glucocorticoid receptors of Amygdala, Hippocampus and prefrontal cortex. Ne ...
... structures: that is Amygdala and Hippocampus, where processing of emotions and memory occurs respectively. During stress the blood brain barrier also becomes more permeable for corticosteroids, resulting in the activation of glucocorticoid receptors of Amygdala, Hippocampus and prefrontal cortex. Ne ...
Recent advances in biosynthesis of fatty acids derived products in
... The activation of acetate is so ATP intensive that it constrains the maximal yield of the acetyl-CoA derived products. In addition, the supply of acetyl-CoA is even more limited under anaerobic conditions, a preferred process for industrial applications. Many prokaryotes contain an acetylating aceta ...
... The activation of acetate is so ATP intensive that it constrains the maximal yield of the acetyl-CoA derived products. In addition, the supply of acetyl-CoA is even more limited under anaerobic conditions, a preferred process for industrial applications. Many prokaryotes contain an acetylating aceta ...
Invariant amino acids essential for decoding function of polypeptide
... controversies may be derived by a methodological distinction between in vivo and in vitro strategies or between genetic versus biochemical approaches. Here, we introduced various amino acid substitutions into four positions in the N domain of human eRF1. Two of them, 55 and 125, are invariant in all ...
... controversies may be derived by a methodological distinction between in vivo and in vitro strategies or between genetic versus biochemical approaches. Here, we introduced various amino acid substitutions into four positions in the N domain of human eRF1. Two of them, 55 and 125, are invariant in all ...
Amino Acid Sequences of Peptides from a Tryptic Digest of a Urea
... 1. A tryptic digest of the protein fraction U.S. 3 from oxidized wool has been separated into 32 peptide fractions by cation-exchange resin chromatography. 2. Most of these fractions have been resolved into their component peptides by a combination of the techniques of eation-exchange resin chromato ...
... 1. A tryptic digest of the protein fraction U.S. 3 from oxidized wool has been separated into 32 peptide fractions by cation-exchange resin chromatography. 2. Most of these fractions have been resolved into their component peptides by a combination of the techniques of eation-exchange resin chromato ...
Relació estructura-funció en la família de transportadors d'aminoàcids heteromultimèrics.
... related to neurotransmission (the GABA transporter GAT1 and the glutamate transporter GLT-1), these data allowed microsequencing or generation of specific antibodies that have been used to isolate cDNA clones (184, 424). Alternative strategies and serendipity allowed the identification of up to 22 c ...
... related to neurotransmission (the GABA transporter GAT1 and the glutamate transporter GLT-1), these data allowed microsequencing or generation of specific antibodies that have been used to isolate cDNA clones (184, 424). Alternative strategies and serendipity allowed the identification of up to 22 c ...
Serine racemases from barley, Hordeum vulgare L., and other plant
... ∗∗ Okayama University, [email protected] This paper is posted at eScholarship@OUDIR : Okayama University Digital Information ...
... ∗∗ Okayama University, [email protected] This paper is posted at eScholarship@OUDIR : Okayama University Digital Information ...
Effects of dietary polyunsaturated fatty acids on
... April until August, whereas, in October, the hibernating group was placed in an environmental chamber (see below). Temperature telemeters were surgically implanted in animals from both groups, as described by Muleme and colleagues (Muleme et al., 2006), with ...
... April until August, whereas, in October, the hibernating group was placed in an environmental chamber (see below). Temperature telemeters were surgically implanted in animals from both groups, as described by Muleme and colleagues (Muleme et al., 2006), with ...
Lactic Acidosis
... It is one of the causes of an increased anion gap metabolic acidosis. Overall critically ill patients with lactic acidosis tend to have high mortality if their lactate levels are significantly elevated (>8 mmols/L). In each individual case however, the actual prognosis will be completely dependent o ...
... It is one of the causes of an increased anion gap metabolic acidosis. Overall critically ill patients with lactic acidosis tend to have high mortality if their lactate levels are significantly elevated (>8 mmols/L). In each individual case however, the actual prognosis will be completely dependent o ...
Purification and Characterization of Two Thermostable Proteases
... inhibitor PMSF, but not by EDTA and thiol reagents such as iodoacetamide, which was specific for true cysteine proteases. Therefore, the two proteases were classified as serine proteases. Proteases are also classified on the basis of their optimum pH of activity (e.g., acidic, neutral, or alkaline p ...
... inhibitor PMSF, but not by EDTA and thiol reagents such as iodoacetamide, which was specific for true cysteine proteases. Therefore, the two proteases were classified as serine proteases. Proteases are also classified on the basis of their optimum pH of activity (e.g., acidic, neutral, or alkaline p ...
Metabolism and function of bile acids
... 3.1. The classical and alternative bile acid biosynthetic pathways The classical pathway operates entirely in the liver (Fig. 4). It begins by c~-hydroxylation of carbon 7 of the cholesterol steroid nucleus. This reaction is catalyzed by the microsomal cytochrome P-450 monooxygenase referred to as c ...
... 3.1. The classical and alternative bile acid biosynthetic pathways The classical pathway operates entirely in the liver (Fig. 4). It begins by c~-hydroxylation of carbon 7 of the cholesterol steroid nucleus. This reaction is catalyzed by the microsomal cytochrome P-450 monooxygenase referred to as c ...
Role of NAD+-Dependent Malate Dehydrogenase in the Metabolism
... 5 mM), glycerate, lactate, α-ketoglutarate, citrate, serine, pyrophosphate (1 mM) or КH2PO4 (10 mM) were tested as potential effectors. To test the effect of divalent metals on the MDH activity, aquatic stock solutions of CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 were added to a final concen ...
... 5 mM), glycerate, lactate, α-ketoglutarate, citrate, serine, pyrophosphate (1 mM) or КH2PO4 (10 mM) were tested as potential effectors. To test the effect of divalent metals on the MDH activity, aquatic stock solutions of CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 were added to a final concen ...
Allicin, a naturally occurring antibiotic from garlic, specifically inhibits
... allicin, but hitherto no target enzyme could be identified. In view of the broad spectrum of effects and sensitive organisms, one would expect that a central point of metabolism must be affected by allicin. Inhibition of lipid and fatty acid biosynthesis causes large changes in the viability of cell ...
... allicin, but hitherto no target enzyme could be identified. In view of the broad spectrum of effects and sensitive organisms, one would expect that a central point of metabolism must be affected by allicin. Inhibition of lipid and fatty acid biosynthesis causes large changes in the viability of cell ...
ANTIBIOTICS
... The chemical structure of sometypical tetracyclines is shown in Fig. 10 and their biosynthesis in Figs. 11 and 12 (McCormick, 1965). Chlortetracycline (CTC) and tetracycline (TC) are produced by the actinomycete Streptomyces aureofaciens, whereas oxytetracycline (OTC) and tetracycline by the actinom ...
... The chemical structure of sometypical tetracyclines is shown in Fig. 10 and their biosynthesis in Figs. 11 and 12 (McCormick, 1965). Chlortetracycline (CTC) and tetracycline (TC) are produced by the actinomycete Streptomyces aureofaciens, whereas oxytetracycline (OTC) and tetracycline by the actinom ...