The Metabolism of Acetate by the Blue-green Algae
... other than carbon dioxide has been noted by many workers. Exceptions to this statement include Tolypothrix tenius, the growth of which is stimulated by glucose (Kiyohara et al. 1960; 1962) and ChlorogZoc.n.fi.itscliii, which has been reported to grow in the dark on sLicrose albeit the growth rate wa ...
... other than carbon dioxide has been noted by many workers. Exceptions to this statement include Tolypothrix tenius, the growth of which is stimulated by glucose (Kiyohara et al. 1960; 1962) and ChlorogZoc.n.fi.itscliii, which has been reported to grow in the dark on sLicrose albeit the growth rate wa ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... acyltransferase family. However, the recent report of the 3-D structures of CrAT and COT has provided valuable insights into the molecular basis of substrate specificity and catalytic activity in the acyltransferase family (Jogl 2003, Jogl 2005). ...
... acyltransferase family. However, the recent report of the 3-D structures of CrAT and COT has provided valuable insights into the molecular basis of substrate specificity and catalytic activity in the acyltransferase family (Jogl 2003, Jogl 2005). ...
... structure, that is, the active amino acid in their reactive site, which are serine-, cysteine-, aspartic-, and metallo-PIs (Losso, 2008). In specially, plant serine-PIs are grouped into Bovine pancreatic trypsin inhibitor (Kunitz), Pancreatic secretory trypsin inhibitor (Kazal), Streptomyces subtili ...
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... Stand-Alone Endocrine Testing ................................................82 ...
... Stand-Alone Endocrine Testing ................................................82 ...
Some Aspects of Fatty Acid Oxidation in Isolated Fat
... 1. Mitochondria were prepared from fat-ells isolated from rat epididymal adipose tissues of fed and 48 h-starved rats to study some aspects of fatty acid oxidation in this tissue. The data were compared with values obtained in parallel experiments with liver mitochondria that were prepared and incub ...
... 1. Mitochondria were prepared from fat-ells isolated from rat epididymal adipose tissues of fed and 48 h-starved rats to study some aspects of fatty acid oxidation in this tissue. The data were compared with values obtained in parallel experiments with liver mitochondria that were prepared and incub ...
Carbon and electron flow in Clostridium butyricum
... glycerol, a more reduced substrate than glucose, requires the disposal of the two extra hydrogen atoms; this could be accomplished by stimulating H, production. Surprisingly, the reducing power is not used to form H,, but rather a more reduced compound, 1,3-PD. The concentrations of the fermentation ...
... glycerol, a more reduced substrate than glucose, requires the disposal of the two extra hydrogen atoms; this could be accomplished by stimulating H, production. Surprisingly, the reducing power is not used to form H,, but rather a more reduced compound, 1,3-PD. The concentrations of the fermentation ...
Identification of a New Glycerol-3-phosphate - UNC
... membrane (microsomal GPAT), and the other is located in the outer mitochondrial membrane (mtGPAT). Although the microsomal GPAT has not been cloned or purified, its activity is easily distinguished, because, unlike mtGPAT, the microsomal isoform is inhibited by sulfhydryl reagents (2). In most tissu ...
... membrane (microsomal GPAT), and the other is located in the outer mitochondrial membrane (mtGPAT). Although the microsomal GPAT has not been cloned or purified, its activity is easily distinguished, because, unlike mtGPAT, the microsomal isoform is inhibited by sulfhydryl reagents (2). In most tissu ...
The Enzymes of Ammonia Assimilation and their
... The activities of GS, NADP-GOGAT and NADP-GDH in extracts of members of the ‘herbicola’, ‘carotovora’ and ‘amylovora’ clusters, grown with different sources of nitrogen, are shown in Table 1. NAD-GOGAT, NAD-GDH and corresponding amidotransferases and dehydrogenases (both NAD- and NADP-linked) able t ...
... The activities of GS, NADP-GOGAT and NADP-GDH in extracts of members of the ‘herbicola’, ‘carotovora’ and ‘amylovora’ clusters, grown with different sources of nitrogen, are shown in Table 1. NAD-GOGAT, NAD-GDH and corresponding amidotransferases and dehydrogenases (both NAD- and NADP-linked) able t ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... 3.2. EXPRESSION OF RAT CARNITINE ACETYLTRANSFERASE IN YEAST Plasmids pYES and pYESCrATwt were transformed in the S. cerevisiae strain FY23∆cat2. Uracil-positive (Ura+) transformants were selected and overexpression of rat CrAT was induced by growth of the cells on a galactose-containing medium, as d ...
... 3.2. EXPRESSION OF RAT CARNITINE ACETYLTRANSFERASE IN YEAST Plasmids pYES and pYESCrATwt were transformed in the S. cerevisiae strain FY23∆cat2. Uracil-positive (Ura+) transformants were selected and overexpression of rat CrAT was induced by growth of the cells on a galactose-containing medium, as d ...
P. gingivalis
... Chronic Periodontitis Chronic periodontitis results from an exuberant inflammation induced by pathogenic oral microorganisms that stimulate host cells to release proinflammatory cytokines and exhibit increased production of Reactive Oxygen Species (ROS) as part of the host response to infection. ...
... Chronic Periodontitis Chronic periodontitis results from an exuberant inflammation induced by pathogenic oral microorganisms that stimulate host cells to release proinflammatory cytokines and exhibit increased production of Reactive Oxygen Species (ROS) as part of the host response to infection. ...
C H A P
... followed by transformation into both E. coli strains. For this reason, the process was repeated using the primers HPESTFW and HPESTBW, designed for the specific amplification of estV ORF and a few nucleotides of the upstream (11 bp, containing the ribosome binding site) and downstream (15 bp) region ...
... followed by transformation into both E. coli strains. For this reason, the process was repeated using the primers HPESTFW and HPESTBW, designed for the specific amplification of estV ORF and a few nucleotides of the upstream (11 bp, containing the ribosome binding site) and downstream (15 bp) region ...
Involvement of Polyamine Catabolism in the Regulation of Glucose
... regulation of glucose, energy and lipid metabolism was investigated using transgenic mice overexpressing or deficient for SSAT. The characterization of SSAT mice revealed that these mice exhibited severely reduced whole body WAT mass, elevated energy expenditure, high insulin sensitivity, a low tiss ...
... regulation of glucose, energy and lipid metabolism was investigated using transgenic mice overexpressing or deficient for SSAT. The characterization of SSAT mice revealed that these mice exhibited severely reduced whole body WAT mass, elevated energy expenditure, high insulin sensitivity, a low tiss ...
Title Biotin Biosynthesis in Microorganisms (Commemoration Issue
... Since early studies of biotin biosynthesis, it has been recognized that DTB is converted to biotin during growth of various microorganisms.62-67> Using resting cells, biotin biosynthesis from DTB was demonstrated with some bacteria64,ss-79) and yeasts.71-74) There have been no studies on enzymic syn ...
... Since early studies of biotin biosynthesis, it has been recognized that DTB is converted to biotin during growth of various microorganisms.62-67> Using resting cells, biotin biosynthesis from DTB was demonstrated with some bacteria64,ss-79) and yeasts.71-74) There have been no studies on enzymic syn ...
Oxidation and Synthesis of Fatty Acids in Soluble Enzyme Systems
... step of fatty acid oxidation, the conversion of the fatty acid to the corresponding coenzyme A derivative (10-12). Coenzyme A (CoA) is a complex nucleotide whose structure (shown in Fig. 1) has recently been established by Lipmann and his group (13, 14). For present purposes it can be conceived of a ...
... step of fatty acid oxidation, the conversion of the fatty acid to the corresponding coenzyme A derivative (10-12). Coenzyme A (CoA) is a complex nucleotide whose structure (shown in Fig. 1) has recently been established by Lipmann and his group (13, 14). For present purposes it can be conceived of a ...
Inhibitory Effects of Neurotransmitters and Steroids on Human
... using an L-7400 UV detector (Hitachi). The mobile phase was 7% acetonitrile containing 0.05% phosphoric acid and 1 mM sodium heptane sulfonate. The retention times of nicotine-⌬5⬘(1⬘)-iminium ion and nicotine were 18 min and 21 min, respectively. Since an authentic standard was not available, the re ...
... using an L-7400 UV detector (Hitachi). The mobile phase was 7% acetonitrile containing 0.05% phosphoric acid and 1 mM sodium heptane sulfonate. The retention times of nicotine-⌬5⬘(1⬘)-iminium ion and nicotine were 18 min and 21 min, respectively. Since an authentic standard was not available, the re ...
Design, Synthesis, and Antibacterial Properties of Dual
... First and foremost, I would like to express my deepest gratitude for my advisor, Dr. Grover Waldrop. His insight, guidance, advice, and support throughout the years have been nothing but beneficial and advantageous to my growth as a scientist. I would also like to thank Dr. Carol Taylor for her unde ...
... First and foremost, I would like to express my deepest gratitude for my advisor, Dr. Grover Waldrop. His insight, guidance, advice, and support throughout the years have been nothing but beneficial and advantageous to my growth as a scientist. I would also like to thank Dr. Carol Taylor for her unde ...
Characterization and the role of carbonic anhydrase
... In cyanobacteria, for the carbon concentrating mechanism to function, there is an absolute requirement for carbonic anhydrase activity within the carboxysome to convert the bicarbonate to carbon dioxide, and a simultaneous requirement that minimal carbonic anhydrase activity be found within the cyst ...
... In cyanobacteria, for the carbon concentrating mechanism to function, there is an absolute requirement for carbonic anhydrase activity within the carboxysome to convert the bicarbonate to carbon dioxide, and a simultaneous requirement that minimal carbonic anhydrase activity be found within the cyst ...
Regulation of Ketone Body Metabolism and the Role of PPAR
... Activated Receptors (PPARs) are important regulators of cellular responses to variable nutrient supply during both fed and fasted states. Acting as transcription factors, and directly modulated by fatty acids and their derivatives, PPARs induce transcription of the proper set of genes, encoding prot ...
... Activated Receptors (PPARs) are important regulators of cellular responses to variable nutrient supply during both fed and fasted states. Acting as transcription factors, and directly modulated by fatty acids and their derivatives, PPARs induce transcription of the proper set of genes, encoding prot ...
CHARACTERIZATION OF B-LACTAMASES FROM TWO B
... steps (Table 2, Fig 2(a.b) and Fig. 3(a,b) indicate that P-lactamase from P.aeruginosa Z 90 was purified at 44.6 fold and the specific activity reached to 18.28 units/mg protein and the yield percentage was reached to 50.15 % while the p-lactamase from P. aeruginosa Z 33 was purified at 42.4 fold an ...
... steps (Table 2, Fig 2(a.b) and Fig. 3(a,b) indicate that P-lactamase from P.aeruginosa Z 90 was purified at 44.6 fold and the specific activity reached to 18.28 units/mg protein and the yield percentage was reached to 50.15 % while the p-lactamase from P. aeruginosa Z 33 was purified at 42.4 fold an ...
The relevance of carbon dioxide metabolism in
... thermophilus showed a different degree of energy status depending on the CO2 availability. In agreement with proteomic data, cells grown under N2 showed a significantly higher milk acidification rate compared with those grown in an enriched CO2 atmosphere. Experiments carried out on S. thermophilus ...
... thermophilus showed a different degree of energy status depending on the CO2 availability. In agreement with proteomic data, cells grown under N2 showed a significantly higher milk acidification rate compared with those grown in an enriched CO2 atmosphere. Experiments carried out on S. thermophilus ...
Malic acid production by Aspergillus oryzae
... concerning yields, rates and titers. This strain can then be used in a biorefinery, which is in analogy to the petroleum refinery producing fuels, power and chemicals, just that the ...
... concerning yields, rates and titers. This strain can then be used in a biorefinery, which is in analogy to the petroleum refinery producing fuels, power and chemicals, just that the ...
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase and
... their activity is linked to the energy status of the cell via the NAD+/NADH ratio (Imai et al., 2000; Houtkooper et al., 2010; Imai and Guarente, 2010). Global proteomic surveys have shown that mitochondrial proteins are extensively modified by lysine acetylation (Kim et al., 2006; Lombard et al., 2 ...
... their activity is linked to the energy status of the cell via the NAD+/NADH ratio (Imai et al., 2000; Houtkooper et al., 2010; Imai and Guarente, 2010). Global proteomic surveys have shown that mitochondrial proteins are extensively modified by lysine acetylation (Kim et al., 2006; Lombard et al., 2 ...
Carbohydrate-Based Mimetics in Drug Design: Sugar Amino Acids
... molecular recognition. In order to perform these functions, the correct folding of the biopolymers creating the active site is crucial, since any kind of interaction is observed only if the reactive groups are positioned in the correct spatial orientation to each other. Thus, the development of smal ...
... molecular recognition. In order to perform these functions, the correct folding of the biopolymers creating the active site is crucial, since any kind of interaction is observed only if the reactive groups are positioned in the correct spatial orientation to each other. Thus, the development of smal ...
Variability of Wax Ester Fermentation in Natural and Bleached
... In the absence of oxygen, the pyruvate stemming from glycolysis in Euglena is reduced to acetyl-CoA by pyruvate:NADP1 oxidoreductase (PNO), an oxygen-sensitive enzyme (Inui et al. 1987; Rotte et al. 2001) that also was recently found in the anaerobic protist Blastocystis (Lantsman et al. 2008). The ...
... In the absence of oxygen, the pyruvate stemming from glycolysis in Euglena is reduced to acetyl-CoA by pyruvate:NADP1 oxidoreductase (PNO), an oxygen-sensitive enzyme (Inui et al. 1987; Rotte et al. 2001) that also was recently found in the anaerobic protist Blastocystis (Lantsman et al. 2008). The ...
Marine Biotecnology
... Some biochemical characteristics are similar to those found in mammalian digestive lipases: molecular masses of ∼40–50 kDa with an alkaline pI between 6.8 and 7.8 (Moreau et al. 1992; Miled et al. 2000). In crustaceans, lipases are liberated by B cells of the midgut gland (Loizzi and Peterson 1971) ...
... Some biochemical characteristics are similar to those found in mammalian digestive lipases: molecular masses of ∼40–50 kDa with an alkaline pI between 6.8 and 7.8 (Moreau et al. 1992; Miled et al. 2000). In crustaceans, lipases are liberated by B cells of the midgut gland (Loizzi and Peterson 1971) ...