Characterization of carnitine and fatty acid metabolism in the long
... acetonitrile, which was stored at − 20 ◦C until analysis. Plasma and urine samples were prepared as described previously [15], except that propylation reagent was used instead of butylation reagent, and samples were stored in acetonitrile at − 20 ◦C until analysis. On the day of analysis, 70 µl of t ...
... acetonitrile, which was stored at − 20 ◦C until analysis. Plasma and urine samples were prepared as described previously [15], except that propylation reagent was used instead of butylation reagent, and samples were stored in acetonitrile at − 20 ◦C until analysis. On the day of analysis, 70 µl of t ...
MONODEHYROASCORBATE REDUCTASE4 Is Required for Seed
... bodies purified from 2-d-old sdp2 seedlings but at a significantly lower rate than from the wild type (Figure 5B). This suggests that peroxidation of substrate and/or oxidation of additional oil body proteins also forms a partial barrier to oil hydrolysis by SDP1. To investigate whether SDP1 suffers ...
... bodies purified from 2-d-old sdp2 seedlings but at a significantly lower rate than from the wild type (Figure 5B). This suggests that peroxidation of substrate and/or oxidation of additional oil body proteins also forms a partial barrier to oil hydrolysis by SDP1. To investigate whether SDP1 suffers ...
Natural amino acids do not require their native tRNAs for efficient
... The involvement of tRNA structural elements beyond the anticodon in aminoacyl-tRNA (aa-tRNA) selection by the ribosome has revealed that substrate recognition is considerably more complex than originally envisioned in the adaptor hypothesis. By combining recent breakthroughs in aa-tRNA synthesis and ...
... The involvement of tRNA structural elements beyond the anticodon in aminoacyl-tRNA (aa-tRNA) selection by the ribosome has revealed that substrate recognition is considerably more complex than originally envisioned in the adaptor hypothesis. By combining recent breakthroughs in aa-tRNA synthesis and ...
Amino acid concentrations in fluids from the bovine oviduct and
... levels of essential amino acids in UF. Ratios of amino acids are known to be critical for protein synthesis in living cells, and the current observation is worth investigating whether the ratio rather than the absolute value of amino acid levels is more important for embryo development. This notion ...
... levels of essential amino acids in UF. Ratios of amino acids are known to be critical for protein synthesis in living cells, and the current observation is worth investigating whether the ratio rather than the absolute value of amino acid levels is more important for embryo development. This notion ...
Transcriptional Regulation of Metabolism
... the main characteristics of the transcription factors belonging to this family. The notion of “metabolic sensor” receptors was more particularly developed with respect to these nuclear receptors, as also explained in Appendix A with the accompanying Figure 1B. Appendixes B–F describe the main featur ...
... the main characteristics of the transcription factors belonging to this family. The notion of “metabolic sensor” receptors was more particularly developed with respect to these nuclear receptors, as also explained in Appendix A with the accompanying Figure 1B. Appendixes B–F describe the main featur ...
C 2 R
... 87 were compared. In most cases Phe87, was mutated to amino acids with small nonpolar side chains (Gly, Ala, Leu, Ile, Val), whereas only two uncharged polar amino acids (Ser, Tyr) were evaluated. Dependent on the substrate tested and the enzyme template, the type of mutation of Phe87 has differenti ...
... 87 were compared. In most cases Phe87, was mutated to amino acids with small nonpolar side chains (Gly, Ala, Leu, Ile, Val), whereas only two uncharged polar amino acids (Ser, Tyr) were evaluated. Dependent on the substrate tested and the enzyme template, the type of mutation of Phe87 has differenti ...
Title Biotin Biosynthesis in Microorganisms (Commemoration Issue
... the reactions from pimelic acid to dethiobiotin were elucidated and found to be of new types. Recent studies on the last step of the pathway are progressingwith intact cells to give a clue to elucidation of the conversion mechanism from dethiobiotin to biotin. The biosynthesisof biotin was found to ...
... the reactions from pimelic acid to dethiobiotin were elucidated and found to be of new types. Recent studies on the last step of the pathway are progressingwith intact cells to give a clue to elucidation of the conversion mechanism from dethiobiotin to biotin. The biosynthesisof biotin was found to ...
Environmental Surveillance of Polioviruses in Rio de - Arca
... Enterovirus RRL/Fiocruz. In the present study, the rate of polio ? non-polio enterovirus isolation in L20B and RD cell lines was 87 %, in agreement with a work by Vinjé et al. (2004), which reported 85 %. The detection of non-polio enteroviruses in the samples is an indication that the environmenta ...
... Enterovirus RRL/Fiocruz. In the present study, the rate of polio ? non-polio enterovirus isolation in L20B and RD cell lines was 87 %, in agreement with a work by Vinjé et al. (2004), which reported 85 %. The detection of non-polio enteroviruses in the samples is an indication that the environmenta ...
Studies on pituitary polypeptide hormones. 2. The action of pepsin
... have been inhibited in the presence of other products of digestion has not been eliminated. Nevertheless, both the N-terminal peptide fragment, T 1 (positions 1-11 in B-MSH), and the C-terminal fragment, S4 (positions 8-18 in pMSH), which contain five and six respectively of the seven amino acid res ...
... have been inhibited in the presence of other products of digestion has not been eliminated. Nevertheless, both the N-terminal peptide fragment, T 1 (positions 1-11 in B-MSH), and the C-terminal fragment, S4 (positions 8-18 in pMSH), which contain five and six respectively of the seven amino acid res ...
The role of hyaluronic acid in protecting surface
... of SAPL is apparently threatened by the lysing activity of phospholipase A2 (PLA2). Methods. The effects of increasing concentrations of HA degraded by free radicals and non-degraded HA on the lysing activity of PLA2 were examined in vitro. Liposomes (lipid model membrane) containing phosphatidylcho ...
... of SAPL is apparently threatened by the lysing activity of phospholipase A2 (PLA2). Methods. The effects of increasing concentrations of HA degraded by free radicals and non-degraded HA on the lysing activity of PLA2 were examined in vitro. Liposomes (lipid model membrane) containing phosphatidylcho ...
Response of glucose metabolism enzymes in an acute porphyria
... observed in glucose metabolism enzymes could be partly related to the damage caused by ROS on their enzymatic protein structures, suggesting that they could be also linked to the beneficial role of glucose administration in acute hepatic porphyria cases. © 2005 Elsevier Ireland Ltd. All rights reser ...
... observed in glucose metabolism enzymes could be partly related to the damage caused by ROS on their enzymatic protein structures, suggesting that they could be also linked to the beneficial role of glucose administration in acute hepatic porphyria cases. © 2005 Elsevier Ireland Ltd. All rights reser ...
PDF hosted at the Radboud Repository of the Radboud University
... large bowel by anaerobic bacteria. It has been shown that these compounds can have tumour-promoting capacities in animal experiments. In epidemiological studies, colonic cancer risk is related to the faecal bile acid concentration. In serum and bile of patients with colonic adenomas, more deoxycholi ...
... large bowel by anaerobic bacteria. It has been shown that these compounds can have tumour-promoting capacities in animal experiments. In epidemiological studies, colonic cancer risk is related to the faecal bile acid concentration. In serum and bile of patients with colonic adenomas, more deoxycholi ...
Cloning and functional characterization of a new subtype of the
... astrocytes is similar to the hepatic system N, whereas the system present in neurons is distinct from the hepatic system N and also from the skeletal muscle system Nm. The neuronal system N, called Nb, also exhibits weak Li⫹ tolerance and pH sensitivity, similar to Nm, but is inhibited by glutamate, ...
... astrocytes is similar to the hepatic system N, whereas the system present in neurons is distinct from the hepatic system N and also from the skeletal muscle system Nm. The neuronal system N, called Nb, also exhibits weak Li⫹ tolerance and pH sensitivity, similar to Nm, but is inhibited by glutamate, ...
Isolation and Chemical Characterization of Plasma Membranes from
... (w/v) sucrose made up as described previously and 40% sucrose layered on top. After centrifugation (2 h, 82 500g, in a SW 27 rotor) the material sedimenting at the interface was collected and pelleted before being resuspended in 40 % sucrose. This was then centrifuged a second time (1-5h at 82 5008 ...
... (w/v) sucrose made up as described previously and 40% sucrose layered on top. After centrifugation (2 h, 82 500g, in a SW 27 rotor) the material sedimenting at the interface was collected and pelleted before being resuspended in 40 % sucrose. This was then centrifuged a second time (1-5h at 82 5008 ...
TINOSPORA CORDIFOLIA AFLATOXIN B INDUCED RENAL DAMAGE
... electrophilic functional groups. They play an important role in detoxification and metabolism of many xenobiotic and endobiotic compounds34. Superoxide dismutase is a very important enzyme that functions as a cellular antioxidant. It is present in cell cytoplasm and mitoch ...
... electrophilic functional groups. They play an important role in detoxification and metabolism of many xenobiotic and endobiotic compounds34. Superoxide dismutase is a very important enzyme that functions as a cellular antioxidant. It is present in cell cytoplasm and mitoch ...
2.2 Sol-gel immobilization
... the binding buffer. Both LipT6M_CE and LipT6M_HT fractions were analyzed for protein content by the Bradford assay (calibrated by using bovine-serum albumin) and used for soluble lipase activity assay, i.e. the hydrolysis of pNPL. The pure lipase was eluted from the column with a linear gradient of ...
... the binding buffer. Both LipT6M_CE and LipT6M_HT fractions were analyzed for protein content by the Bradford assay (calibrated by using bovine-serum albumin) and used for soluble lipase activity assay, i.e. the hydrolysis of pNPL. The pure lipase was eluted from the column with a linear gradient of ...
New Reactions in the Crotonase Superfamily: Structure of
... dehalogenase, a conserved Asp residue serves either as an acid/base catalyst (Asp 204 in the isomerase) or as a nucleophile (Asp 145 in the dehalogenase). However, for many members of the superfamily, the correlation between structure and function is still too ill-defined to allow either the predict ...
... dehalogenase, a conserved Asp residue serves either as an acid/base catalyst (Asp 204 in the isomerase) or as a nucleophile (Asp 145 in the dehalogenase). However, for many members of the superfamily, the correlation between structure and function is still too ill-defined to allow either the predict ...
Induction of type-IIA secretory phospholipase A2 in animal models of
... Type-IIA secretory phospholipase A2 activity and surfactant phospholipid quantitation. Alveolar instillation of LPS or live bacteria led to a similar level of neutrophil influx into alveolar spaces and decreased static compliance. During both injuries, sPLA2-IIA messenger ribonucleic acid (mRNA) was ...
... Type-IIA secretory phospholipase A2 activity and surfactant phospholipid quantitation. Alveolar instillation of LPS or live bacteria led to a similar level of neutrophil influx into alveolar spaces and decreased static compliance. During both injuries, sPLA2-IIA messenger ribonucleic acid (mRNA) was ...
Glucose utilization by Streptomyces griseus
... principal carbohydrate with certain carbohydrates from the soybean mealsucrose, raffinose,stachyose (Street & Bailey, 1915; Sato, 1921). After sterilization some material resembling fructose is present. Cultures were harvested in triplicate at regular intervals and the residual carbohydrate determin ...
... principal carbohydrate with certain carbohydrates from the soybean mealsucrose, raffinose,stachyose (Street & Bailey, 1915; Sato, 1921). After sterilization some material resembling fructose is present. Cultures were harvested in triplicate at regular intervals and the residual carbohydrate determin ...
Fatty acids stimulate insulin secretion from human pancreatic
... human islets in the presence of fasting glucose concentration and that MUFAs were more potent than SFAs made us study the secretory response dynamically by perifusing human islets. Perifused human islets secreted insulin at 2.09 ± 0.12 fmol/min/μg protein in the presence of 5.5 mmol/l glucose (Fig. ...
... human islets in the presence of fasting glucose concentration and that MUFAs were more potent than SFAs made us study the secretory response dynamically by perifusing human islets. Perifused human islets secreted insulin at 2.09 ± 0.12 fmol/min/μg protein in the presence of 5.5 mmol/l glucose (Fig. ...
J B , Mar. 2004, p. 1531–1536 Vol. 186, No. 5
... of geranylgeranyldiphosphate (GGDP; C20PP), a reaction catalyzed by the carotenoid synthase CrtB (Fig. 1). The vast majority of the ⬎700 known carotenoids (9) arise as a result of different types and levels of modification of the C40 backbone, catalyzed by promiscuous (downstream) carotenoid biosynt ...
... of geranylgeranyldiphosphate (GGDP; C20PP), a reaction catalyzed by the carotenoid synthase CrtB (Fig. 1). The vast majority of the ⬎700 known carotenoids (9) arise as a result of different types and levels of modification of the C40 backbone, catalyzed by promiscuous (downstream) carotenoid biosynt ...
Characterization of a Regular Array in the Wall of
... Isolation of regularly arranged subunits and preparation of wall fragments free of the subunits. The regularly arranged subunits were isolated from the wall fraction of L. buchneri by extraction with 4 M-guanidine hydrochloride (GHCl) as described previously (Masuda & Kawata, 1980). The resulting fr ...
... Isolation of regularly arranged subunits and preparation of wall fragments free of the subunits. The regularly arranged subunits were isolated from the wall fraction of L. buchneri by extraction with 4 M-guanidine hydrochloride (GHCl) as described previously (Masuda & Kawata, 1980). The resulting fr ...
Hydrolysisof Glutathioneby Human Liver `y
... conjugates were the substrate, the enzyme source was kidney. The kidney is now considered in the rat-and, by implication, in man-to be the major site of catabolism of glutathione and glutathione conjugates (4, 7). In this work we demonstrate that human liver y-glutamyltransferase has kinetic propert ...
... conjugates were the substrate, the enzyme source was kidney. The kidney is now considered in the rat-and, by implication, in man-to be the major site of catabolism of glutathione and glutathione conjugates (4, 7). In this work we demonstrate that human liver y-glutamyltransferase has kinetic propert ...
Science Course Outline Template
... have tidied and cleaned your equipment and workspace as instructed by the demonstrators and technical staff. ...
... have tidied and cleaned your equipment and workspace as instructed by the demonstrators and technical staff. ...
Transamination and asymmetry in glutamate transport across the
... amino acid glutamate (the concentration of glutamate in the plasma of Rana c a t e s b e i a n a is about 90 pM; Boyd, 1976), while the high intraceilular level of glutamate will favour transamination to alanine. Since the basolateral membrane transport of alanine appears to be symmetrical, both exi ...
... amino acid glutamate (the concentration of glutamate in the plasma of Rana c a t e s b e i a n a is about 90 pM; Boyd, 1976), while the high intraceilular level of glutamate will favour transamination to alanine. Since the basolateral membrane transport of alanine appears to be symmetrical, both exi ...
Hepoxilin
Hepoxilins (HxA3 and HxB3) are nonclassic eicosanoid hormones involved in inflammation.