Development and evaluation of a reporter system for
... Reporter genes are commonly used in the construction of transcriptional fusions to regulatory elements of interest, and have facilitated greatly the analysis of gene expression and the study of individual promoters and their regulation. Moreover, the construction of translational fusions have also a ...
... Reporter genes are commonly used in the construction of transcriptional fusions to regulatory elements of interest, and have facilitated greatly the analysis of gene expression and the study of individual promoters and their regulation. Moreover, the construction of translational fusions have also a ...
Chapter 25 Amino Acids, Peptides, and Proteins
... Amino acids in which the two functional groups are separated by exactly one carbon atom are called _______ amino acids. Amino acids are coupled together by amide linkages called ____________ bonds. Relatively short chains of amino acids are called ___________. Only twenty amino acids are abundantly ...
... Amino acids in which the two functional groups are separated by exactly one carbon atom are called _______ amino acids. Amino acids are coupled together by amide linkages called ____________ bonds. Relatively short chains of amino acids are called ___________. Only twenty amino acids are abundantly ...
Acid Base Equilibrium Diploma Questions
... ____ 52. In the reaction represented by equation III, the yield of nitric acid coud be increased by a. replacing the mesh in the system with small platinum pellets b. decreasing the total pressure of the system by increasing the volume c. increasing the total pressure of the system by decreasing the ...
... ____ 52. In the reaction represented by equation III, the yield of nitric acid coud be increased by a. replacing the mesh in the system with small platinum pellets b. decreasing the total pressure of the system by increasing the volume c. increasing the total pressure of the system by decreasing the ...
Plant physiology
... in cytoplasmatic lipid droplets (CLDs; Murphy, 2012), although in some cases, such as in Chlamydomonas reinhardtii starchless mutants, they also accumulate in chloroplasts (Fan et al., 2011; Goodson et al., 2011). Recent studies indicate that the CLDs are closely associated with ER membranes and pos ...
... in cytoplasmatic lipid droplets (CLDs; Murphy, 2012), although in some cases, such as in Chlamydomonas reinhardtii starchless mutants, they also accumulate in chloroplasts (Fan et al., 2011; Goodson et al., 2011). Recent studies indicate that the CLDs are closely associated with ER membranes and pos ...
A Substrate Serves as a Hydrogen Atom Donor in the Enzyme
... analyzed by SP-HPLC (Figure 5). Since OXOD is formed by dehydration of HPODE and can be reduced to hydroxyoctadecadienoic acid (HODE) by NaBH4 but not by TPP, the distribution of products was expected to depend on the choice of reducing agent. As shown in Table 2, the relative proportions of 13-(9Z, ...
... analyzed by SP-HPLC (Figure 5). Since OXOD is formed by dehydration of HPODE and can be reduced to hydroxyoctadecadienoic acid (HODE) by NaBH4 but not by TPP, the distribution of products was expected to depend on the choice of reducing agent. As shown in Table 2, the relative proportions of 13-(9Z, ...
A New Subfamily of Major Intrinsic Proteins in
... three novel MIP-like genes, T6K12.29, MRG7.25, and F24I3.30, all very different from any of the previously described plant MIP genes belonging to the PIP, TIP, and NIP subfamilies (U. Johanson at the MIP 2000 meeting in Göteborg, Sweden, unpublished data). Phylogenetic comparison with Arabidopsis r ...
... three novel MIP-like genes, T6K12.29, MRG7.25, and F24I3.30, all very different from any of the previously described plant MIP genes belonging to the PIP, TIP, and NIP subfamilies (U. Johanson at the MIP 2000 meeting in Göteborg, Sweden, unpublished data). Phylogenetic comparison with Arabidopsis r ...
Prostatic acid phosphatase in serum and semen of dogs
... which indicated high heterogeneity of the individual values, maybe due to a sub clinical non-prostatic disease. It could suggest that the substrate used to measure the PAP concentration was not very specific. The PAP values in the serum were lower than those obtained in some other studies (Corazza e ...
... which indicated high heterogeneity of the individual values, maybe due to a sub clinical non-prostatic disease. It could suggest that the substrate used to measure the PAP concentration was not very specific. The PAP values in the serum were lower than those obtained in some other studies (Corazza e ...
13synthesis
... * Degradation of Phospholipids enzyme responsible for degradation are present in all tissues and pancreatic juice. - phospholipases hydrolyze the esters of phosphodiester bonds of phosphoglycerides. * Degradation of Sphingomyelin - Sphingolipids are degraded by sphingomyelinase which remove phospho ...
... * Degradation of Phospholipids enzyme responsible for degradation are present in all tissues and pancreatic juice. - phospholipases hydrolyze the esters of phosphodiester bonds of phosphoglycerides. * Degradation of Sphingomyelin - Sphingolipids are degraded by sphingomyelinase which remove phospho ...
for students of the Faculty of Medicine
... organisms. They are derived from organic acids, in which a hydrogen atom most often located near the α-carbon is substituted by the amino group. Some amino acids have two amino groups located at different carbon atoms, a few contain two or even three carboxyl groups. Two amino acids, proline and its ...
... organisms. They are derived from organic acids, in which a hydrogen atom most often located near the α-carbon is substituted by the amino group. Some amino acids have two amino groups located at different carbon atoms, a few contain two or even three carboxyl groups. Two amino acids, proline and its ...
Molecular analysis of Physarum haemagglutinin I
... O u r previous study ( M o r i t a & Nishi, 1992) reported t h a t plasmodia of P. polycephalum produce two types of lectins, haemagglutinins I a n d 11, which migrate t o a b o u t 6 a n d 11 kDa u p o n Tricine-SDS-PAGE, respectively. In this study, direct E d m a n analysis of t h e b a n d migra ...
... O u r previous study ( M o r i t a & Nishi, 1992) reported t h a t plasmodia of P. polycephalum produce two types of lectins, haemagglutinins I a n d 11, which migrate t o a b o u t 6 a n d 11 kDa u p o n Tricine-SDS-PAGE, respectively. In this study, direct E d m a n analysis of t h e b a n d migra ...
Advantages of compound-specific stable isotope
... (glycine, valine, tyrosine and lysine) to soil of a Plantago lanceolata monoculture. Root and shoot samples were collected 24 h after label application and the isotope composition of the plant tissues was investigated using bulk and CSI measurements. Enrichment of 13C in the case of CSI measurements ...
... (glycine, valine, tyrosine and lysine) to soil of a Plantago lanceolata monoculture. Root and shoot samples were collected 24 h after label application and the isotope composition of the plant tissues was investigated using bulk and CSI measurements. Enrichment of 13C in the case of CSI measurements ...
Differences in the amino acid composition of muscles from pheasant
... acids analysed occurred in breast muscles from pheasants at a higher level as compared with broiler chickens. These differences were tested statistically highly significant (P ≤ 0.01). In the case of phenylalanine and lysine, the difference between the levels found in breast muscles of pheasants and ...
... acids analysed occurred in breast muscles from pheasants at a higher level as compared with broiler chickens. These differences were tested statistically highly significant (P ≤ 0.01). In the case of phenylalanine and lysine, the difference between the levels found in breast muscles of pheasants and ...
Molecular analysis of Physarum haemagglutinin I
... O u r previous study ( M o r i t a & Nishi, 1992) reported t h a t plasmodia of P. polycephalum produce two types of lectins, haemagglutinins I a n d 11, which migrate t o a b o u t 6 a n d 11 kDa u p o n Tricine-SDS-PAGE, respectively. In this study, direct E d m a n analysis of t h e b a n d migra ...
... O u r previous study ( M o r i t a & Nishi, 1992) reported t h a t plasmodia of P. polycephalum produce two types of lectins, haemagglutinins I a n d 11, which migrate t o a b o u t 6 a n d 11 kDa u p o n Tricine-SDS-PAGE, respectively. In this study, direct E d m a n analysis of t h e b a n d migra ...
Full Text - International Journal of Livestock Research
... to be a valuable animal model for insights into immune function. In particular, the development of B cells in this unique organ, the bursa of Fabricius, has provided a novel opportunity to study B cell development (Funk and Thompson, 1996). The bursa also functions as a peripheral gut-associated lym ...
... to be a valuable animal model for insights into immune function. In particular, the development of B cells in this unique organ, the bursa of Fabricius, has provided a novel opportunity to study B cell development (Funk and Thompson, 1996). The bursa also functions as a peripheral gut-associated lym ...
Anal. Chem. (2011) - American Chemical Society
... In the echem WY-cleavage method, electrochemical oxidation of proteins induces cleavage at the amino acid residues Y and W.11-14 Recently, this nonenzymatic and reagentless digestion approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative ...
... In the echem WY-cleavage method, electrochemical oxidation of proteins induces cleavage at the amino acid residues Y and W.11-14 Recently, this nonenzymatic and reagentless digestion approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative ...
Glucose-Regulated Anaplerosis and Cataplerosis in
... second and third pathways. However, the hypothesis has been proposed that anaplerosis, the refilling of Krebs cycle intermediates, is implicated in the KATP-independent pathway(s) (1,6,7), perhaps via malonyl-CoA formation and lipid esterification processes (8) or through a pyruvate/malate shuttle ( ...
... second and third pathways. However, the hypothesis has been proposed that anaplerosis, the refilling of Krebs cycle intermediates, is implicated in the KATP-independent pathway(s) (1,6,7), perhaps via malonyl-CoA formation and lipid esterification processes (8) or through a pyruvate/malate shuttle ( ...
Psoriasis - csnanatomy3 / FrontPage
... Types- Guttate [GUH-tate] Psoriasis • Appears in small, red, individual spots on the skin, normally the trunk or limbs. • Not as thick as plaque legions • A few examples of triggers for Guttate Psoriasis are upper respiratory infections, strep throat, stress and injury to the skin. • Treatments can ...
... Types- Guttate [GUH-tate] Psoriasis • Appears in small, red, individual spots on the skin, normally the trunk or limbs. • Not as thick as plaque legions • A few examples of triggers for Guttate Psoriasis are upper respiratory infections, strep throat, stress and injury to the skin. • Treatments can ...
Purification, Identification and Characterisation of - DORAS
... absolutely necessary therefore showing high primary substrate specificity for the Pro-X bond, while a preference for a hydrophobic residue at the C-terminal end of the scissile bond (P1ʹ′) was evident. The enzyme also showed complete insensitivity to the prolyl oligopeptidase specific inhibitors, JT ...
... absolutely necessary therefore showing high primary substrate specificity for the Pro-X bond, while a preference for a hydrophobic residue at the C-terminal end of the scissile bond (P1ʹ′) was evident. The enzyme also showed complete insensitivity to the prolyl oligopeptidase specific inhibitors, JT ...
"Redundancy " & "Junk" for Codons
... A third type of disease prion disease all centers around the tRNA bringing the correct amino acid along with its correct directions in terms of amount (expression) & longevity (dwell time)for the ribosome to make the protein possessing the correct structure/information-function [as] dictated by the ...
... A third type of disease prion disease all centers around the tRNA bringing the correct amino acid along with its correct directions in terms of amount (expression) & longevity (dwell time)for the ribosome to make the protein possessing the correct structure/information-function [as] dictated by the ...
Modelling malic acid accumulation in fruits: relationships
... involved in the accumulation of both. This paper, which focuses on malic acid, is part of a larger study about modelling the processes involved in the elaboration of fruit acidity (Lobit et al., 2002, 2003). Data about the evolution of organic acid concentrations during fruit development have been p ...
... involved in the accumulation of both. This paper, which focuses on malic acid, is part of a larger study about modelling the processes involved in the elaboration of fruit acidity (Lobit et al., 2002, 2003). Data about the evolution of organic acid concentrations during fruit development have been p ...
o`level chemistry: mcq - Biochemistry Olympiad Website
... aggregates of amino acids (b) drops of oil or dirt that surrounds the molecule (c) a tadpole shaped fatty acid (d) cluster of soap molecules surrounding the dirt particle Answer: (d) Question 14: A diamond does not conduct electricity because: (a) its structure is very compact. (b) only carbon atoms ...
... aggregates of amino acids (b) drops of oil or dirt that surrounds the molecule (c) a tadpole shaped fatty acid (d) cluster of soap molecules surrounding the dirt particle Answer: (d) Question 14: A diamond does not conduct electricity because: (a) its structure is very compact. (b) only carbon atoms ...
Fermentation Processes Using Lactic Acid Bacteria
... (Delves-Broughton, 1990). Another commercially produced bacteriocin is pediocin PA-1 produced by Pediococcus acidilactici and marketed as ATTA TM 2431 (Kerry Bioscience, Carrigaline, Co, Cork, Ireland). The source of natural or controlled microbiota and/or antimicrobial compounds could be traditiona ...
... (Delves-Broughton, 1990). Another commercially produced bacteriocin is pediocin PA-1 produced by Pediococcus acidilactici and marketed as ATTA TM 2431 (Kerry Bioscience, Carrigaline, Co, Cork, Ireland). The source of natural or controlled microbiota and/or antimicrobial compounds could be traditiona ...
Tissue Engineered Human Skin Equivalents
... toxicity screening. Animal testing of cosmetic ingredients is strictly limited in the European Union; even if no alternative tests are available, the majority of the animal tests are banned [25]. Human cadaver skin and excised animal skin have been traditionally used as topical and transdermal perme ...
... toxicity screening. Animal testing of cosmetic ingredients is strictly limited in the European Union; even if no alternative tests are available, the majority of the animal tests are banned [25]. Human cadaver skin and excised animal skin have been traditionally used as topical and transdermal perme ...
Arachidonic Acid and Docosahexaenoic Acid Suppress Osteoclast Formation and Activity
... carbons and 2 double bonds that can be categorized into two principal families, namely: ω-3 LCPUFAs and ω-6 LCPUFAs [4]. The anti-inflammatory ω-3 LCPUFAs are derived from αlinolenic acid (ALA) while the pro-inflammatory ω-6 LCPUFAs are derived from linoleic acid (LA) [4, 8, 12, 13]. The human body ...
... carbons and 2 double bonds that can be categorized into two principal families, namely: ω-3 LCPUFAs and ω-6 LCPUFAs [4]. The anti-inflammatory ω-3 LCPUFAs are derived from αlinolenic acid (ALA) while the pro-inflammatory ω-6 LCPUFAs are derived from linoleic acid (LA) [4, 8, 12, 13]. The human body ...
Fatty Acids :biosynthesis
... additional enzymes. Same products plus one propionyl CoA. • Unsaturated fatty acids require additional enzymes. Same products, but less energy, compared to O saturated fatty acid the same length. CH3 CH2 C SCoA • See table 12.1 for a list of common propionyl CoA fatty acids. ...
... additional enzymes. Same products plus one propionyl CoA. • Unsaturated fatty acids require additional enzymes. Same products, but less energy, compared to O saturated fatty acid the same length. CH3 CH2 C SCoA • See table 12.1 for a list of common propionyl CoA fatty acids. ...
Hepoxilin
Hepoxilins (HxA3 and HxB3) are nonclassic eicosanoid hormones involved in inflammation.