Download zn-1 (Only cell products will be distributed

zn-1
(Only cell products will be distributed.)
INVESTIGATOR
Name
Bill Trevarrow
Address Institute of Neuroscience, University of Oregon 1254; Eugene, OR 97403-1254
E-Mail [email protected]
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen (CC)
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
1-6: 2 day old ZF embryo membranes
Zebrafish (ZF)
mouse
Balb/c
female
spleen
1
13vi84
0.5
IP
Freund’s
2
16vii84
0.5
IP
Freund’s
3
12viii84
0.5
IP
Freund’s
4
18xii84
0.4
IP
Freund’s
5
1vii85
0.25/IP
0.05/IV
none
6
3vii85
0.05
IV
none
4vii85
mouse
SP 2/0
IgG1, mouse
cytoplasmic
good, all neurons, hair cells
not tried
Pos.: zebrafish embryos/larva (2d-4d)
Neg.: Xenopus, betta, trout, medaka, shark, chick, Haplochromis burtoni
ANTIGEN
Chemical properties
?
Molecular weight
~43kDa
Characterization
Immunoprecipitation
not done
Immunoblotting
yes
Purification
not done
Amino acid sequence analysis
Functional effects
not tried
Immunohistochemistry
4% paraformaldehyde, 4C, ON, cryostat, or wholemount
PUBLICATIONS :
Hanneman, E., Trevarrow, B., Metcalfe, W. K., Kimmel, C. B., and Westerfield, M. (1988). Segmental pattern of development of the
hindbrain and spinal cord of the zebrafish embryo. Development 103, 49-58.
Trevarrow, B., Marks, D.L., and Kimmel, C.B. (1990). Organization of hindbrain segments in the zebrafish embryo. Neuron 4, 669679.
(Continued)
zn-1 (Continued)
Kornblum, H.I., Corwin, J.T., and Trevarrow, B. (1990). Selective labeling of sensory hair-cells and neurons in auditory, vestibular,
and lateral line systems by a monoclonal antibody. J. Comp. Neur. 301(2), 162-170.
Larison, K. and Trevarrow, B. (sources)- (1994). Zebrafish monoclonal antibodies. in: The Zebrafish Book. Monte Westerfield
University of Oregon Press; edition 2.1.
Hutchinson, S.A., and Eisen, J.S. (2006). Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify
motoneuron subtype identity. Development 133, 2137-2147.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242