Download 9B2.1 anti-glass INVESTIGATOR Name Gerald M. Rubin

9B2.1 anti-glass
INVESTIGATOR
Name
Gerald M. Rubin
Address Molecular & Cell Biology, University of California at Berkeley, 539 LSA/GM Lab, Berkeley, CA 94720-3200
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
glass-trp E fusion protein (Moses and Rubin 1991)
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
mouse balb/c
P3 x 63Ag8.653
IgG1, kappa light chain
9B2.1 Ab recognizes the Drosophila protein glass. Glass is expressed at high levels in the
nucleus of the developing photoreceptor cells in a sequence that reflects their acquisition of
neuronal identity. 9B2.1 gives strong staining of the morphogenetic furrow and all of the
cells that arise from the furrow. It works well on Westerns.
ANTIGEN
Use PLP fix only.
Chemical properties
Molecular weight
Characterization
Immunoprecipitation
Glass Ag in tissues is apparently methanol sensitive.
Immunoblotting
Purification
Amino acid sequence analysis
Functional effects
Immunohistochemistry
PUBLICATIONS :
Ellis, M.C., O'Neill, E.M., and Rubin, G.M. (1993). Expression of Drosophila glass protein and evidence for negative
regulation of its activity in non-neuronal cells by another DNA-binding protein. Development 119, 855-865.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242