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LA4 INVESTIGATOR Name Thomas M. Jessell and Jane Dodd Address Center for Neurobiology & Behavior, Columbia University, 722 W. 168th St., New York, NY 10032 IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation AR42J cells mouse BALB/c female spleen 10/5/83, 10/19/83, 11/11/83 4.5 x 107 whole cells i.p. complete Freund’s adjuvant followed by incomplete Freund’s adjuvant 11/14/83 mouse NS1 MONOCLONAL ANTIBODY Isotype IgM Specificitycell binding: AR42J cells, human RBC/B group antigen Cell binding Immunohistology Antibody competition Species Specificity rodent ANTIGEN Chemical properties glycolipid; the epitope is an alpha-galactose extended lactoseries carbohydrate that may also Molecular weight be present on proteins Characterization Immunoprecipitation Immunoblotting several bands on TLC Purification Amino acid sequence analysis Functional effects Immunohistochemistry subsets of peripheral sensory structures PUBLICATIONS : Dodd, J., and Jessell, T.M. (1985). Lactoseries carbohydrates specify subsets of dorsal root ganglion neurons projecting to the superficial dorsal horn of rat spinal cord. J. Neurosci. 5, 3278-3294. Chou, D.K.H., Dodd, J., Jessell, T.M., Costello, C.E., and Jungalwala, F.B. (1989). Identification of alpha-galactose (alpha-fucose)-asialo-GM1 glycolipid expressed by subsets of rat dorsal ganglion neurons. J. Biol. Chem. 264, 3409-3415. Lee, C.J., Kong, H., Manzini, M.C., Albuquerque, C., Chao, M.V., and MacDermott, A.B. (2001). Kainate receptors expressed by a subpopulation of developing nociceptors rapidly switch from high to low Ca2+ permeability. J. Neurosci. 21(13), 4572-4581. ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: “The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.” Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242