Download Purified Sp1 protein

Purified Sp1 protein
Catalog No: 31137
Expressed In: HeLa cells
Quantity: 2 µg
Source: Human
Buffer Contents: 2 µg of Purified Sp1 protein and Dilution Buffer AM1 (20 mM TrisCl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is
supplied at 0.08 µg/µl in Dilution Buffer AM1.
Background: Sp1 (specificity protein 1) is a human transcription factor involved in
gene expression in the early development of an organism. The protein is 785
amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor
contains a zinc finger protein motif, by which it binds directly to DNA and
enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind
the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element).
It was discovered in 1983 and has since been modified to form Sp1C, which has a
zinc finger protein DNA binding domain.
Protein Details: The natural Sp1 fraction (accession number NM 138473) was
purified from HeLa cell nuclear extract by using wheat germ agglutinin affinity
chromatography. The purified protein is greater than 95% homogeneous and
contains no detectable protease, DNase or RNase activity.
Application Notes: Purified Sp1 is suitable for in vitro transcription, DNase I
protection and gelshift assays. 50-100 ng is sufficient for reconstituted
transcription assays and 5-25 ng in a 20 µl volume are sufficient for gelshift
assays. The molecular weight of the protein is ~105 kDa. This protein is not
®
recommended for TransAM assays.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and
should be avoided.
Storage and Guarantee: This product is guaranteed for 6 months from date of
receipt.
This product is for research use only and is not for use in diagnostic procedures.
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