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Download Prof. Dinko Mitrecic, MD, PhD Laboratory for Stem Cells
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Department of Medical and Clinical Biophysics Faculty of Medicine Pavol Jozef Šafárik University in Košice, Slovakia Expertise in: • Mass spectrometry based proteomic analysis – top down and bottom-up approach, label free quantification and statistical analysis in proteomics, multidimensional protein/peptide separation including 2DnanoHPLC and 2D electrophoresis, post translational modification analysis with ETD fragmentation. • Mass spectrometers: MALDI TOF (Ultraflextreme Bruker), ion trap (Amazon speed ETD Bruker), QTOF (Agilent 6538), QQQ (Agilent 6460) – all coupled with nanoHPLC. • Proteome profiling of body fluids – blood (specifically platelets and leukocytes), urine, saliva and cerebrospinal fluid. • Proteome profiling of cell lines - breast cancer cell line MCF7 and stem cells. • Cell cultures characterization - monitoring off cell proliferation, morphology change, attachment quality in a label-free and real-time manner • Isolation of stem cells from chorion and bone marrow and their purification by magnetic nanoparticles. https://www.ncbi.nlm.nih.gov/pubmed/27620743 https://www.ncbi.nlm.nih.gov/pubmed/26802999 https://www.ncbi.nlm.nih.gov/pubmed/26772123 https://www.ncbi.nlm.nih.gov/pubmed/26543081 https://link.springer.com/article/10.1007/s10337-017-3246-x Expected benefits and activities during participation in BIONECA: • We will share our experiences with groups working in the field of proteomics and LC-MS based analysis (e.g. method development, proteomic raw data analysis by different softwares, etc) • We will share our experiences and protocols in stem cells isolations from chorion membrane and bone marrow, purification/separation of isolated stem cells by magnetic nanoparticles. • Would like to learn from groups who are experienced in bioinformatics,especially signal pathways analysis by different open source softwares. • Would like to learn about surface/bulk modification of biodegradabile nanomaterials and their homogeneity analysis. • Would like to learn about targeted treatment in cancer therapy by using nanoparticles
