Download Bio 313 worksheet 14 - Iowa State University

BIO 313 SI
WORKSHEET 14
Supplemental Instruction
Iowa State University
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For the following state whether it is a characteristic of Prokaryotes, Eukaryotes, or both
1. Transcription occurs in the nucleus and translation in the cytoplasm
2. Able to utilize post-transcriptional control
3. Transcription unit contains promoter, RNA coding region, and terminator
4. Transcription and translation are coupled events
5. Contain introns that are spliced out
6. Mature RNA has a polyA tail and 5’ cap
7. RNA is often polycistronic
8. Possesses colinearity
9. May contain a Rho factor
10. Undergoes splicing during mRNA processing
11. What is an operon?
12. The process of capping, polyadenylation, and splicing is collectively termed what?
13. What is capping? Explain what happens and where at on the RNA molecule
14. Why does capping occur?
15. What is polyadenylation?
16. Write out of the polyA consensus sequence
17. What is the purpose of the polyA tail? (2 things)
18. Where is the 3’ cleavage site in relation to the polyA consensus sequence? Use
upstream/downstream and say approximately how many bases
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19. Diagram 3’ cleavage and polyadenylation. Include the consensus sequence. Do this in 4
steps with the first step being the DNA molecule. Second step being the primary transcript
20. What is the purpose of pre-mRNA splicing
21. What ribonucleoprotein carries out splicing?
22. What part of the cell does splicing take place?
23. (#27 from book) What would be the most likely effect of a mutation at the following
locations in an E. coli gene?
a. -8
b. -35
c. -20
d. Start site of transcription
24. (#30 from book) Suppose that a mutation occurs in the middle of a large intron of a gene
encoding a protein. What will the most likely effect of the mutation be on the amino acid
sequence of that protein? Explain