Download Annexure `CD-01` L T P/S SW/FW TOTAL CREDIT UNITS 3 0 2 0 4

Annexure ‘CD-01’
Course Title: Basic and Applied Immunology
Course Code:
Credit Units: 4
Course Level: PG (M.Sc)
L
T
P/S
SW/FW
TOTAL
CREDIT
UNITS
3
0
2
0
4
Course Objectives:
Theory: To develop basic understanding of immunology and its importance in better understanding of cancer.
Practical: To have laboratory experience of immunology techniques used in cancer research.
Prerequisites: Basic knowledge of cell biology, and biochemistry.
Course Contents/Syllabus:
Module I: Immunology and Immune Cells
Types of immunity - humoral and cellular immunity, Innate and adaptive immunity, Physiology of immune response,
Specificity and memory, Hematopoiesis and differentiation, Primary and secondary lymphoid organs.
Cells of the immune system: T-cells, B-cells, NK cells, antigen-presenting cells, phagocytes, mast cells, Clonal selection
theory, CD markers.
Module II: Antigen-Antibody Interactions
Antibody: Basic structure and functions, Types of antibody, Antibody gene organization and diversity, Antibody receptors,
Antibody class switching, Polyclonal vs. monoclonal antibodies.
Antigens: nature and behavior, Properties and types of antigen, Antigen-antibody reactions: affinity and avidity, Immunization
and antibody response.
Module III: Antigen Recognition and Presentation
Major Histocompatibility Complex (MHC) - Structure, HLA in human health and diseases – HLA typing and cross matching,
Ontogeny and T-cell receptors, Genomic organization of TCR locus, Antigen processing and presentation of T-lymphocytes
(CD4+ and CD8+), Cross-presentation by MHC-I and class II
Module IV: Mechanism of Immune Response
Complement system, Lymphocyte Biology: Development, differentiation and activation of lymphocytes, Cytokines and cell
Weightage (%)
15% Weightage
20% Weightage
15% Weightage
20% Weightage
signaling, Cell-mediated cytotoxicity, Homeostatic Regulation of Immune Responses, Immune tolerance. Allergy and
Hypersensitivity: Allergic responses, Hypersensitivity reactions (type I and type II), Role of cytokines, IgE, Role of Mast cells,
Eosinophils, and Basophils
Autoimmune diseases: Organ-specific and Systemic autoimmune diseases with appropriate examples
Module V: Immunology and Cancer
Immunological basis of cancer, Role of different immune cells in Cancer, Cancer immuno-surveillance, Innate and acquired
immune responses to cancer, Role of tumor microenvironment. Immunological escape of cancer cells.
Module VI: Cancer Immunotherapy and Cancer Vaccines
Immunological approaches for cancer therapy – Antibody and cell-based therapies. Cancer Vaccines – types, design and
current status.
Student Learning Outcomes: At the end of this course, the students will be able to develop:
 Basic understanding of immunological principles with specific applications in cancer research.
 Hands-on experience for immunology techniques used in biomedical research with special focus on cancer.
Pedagogy for Course Delivery:
Lectures: 43 sessions
Presentation / Seminar: Nil
Class Test: 2 sessions
Total: 45 sessions
Practical: 28 sessions
Class Test: 2 sessions
Total: 30 sessions
Lab/ Practical details, if applicable:
List of Experiments
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Blood grouping by agglutination
Radial Immunodiffusion assays – SRID and DRID
Immuno-electrophoresis
ELISA – Dot, Regular, Sandwich
Immunohistochemistry
Isolation of peripheral blood mononuclear cells
Blood smear preparation
Staining of immune cells present in blood
WIDAL test for typhoid
Lymphoid organs and their microscopic organization
15% Weightage
15% Weightage


FACS / MACS
HLA Matching
Assessment/ Examination Scheme:
Lab/Practical/Studio
(%)
Theory L/T (%)
67%
End Term Examination
33%
100%
Theory Assessment (L&T):
Continuous Assessment/Internal Assessment
Components
(Drop down)
Weightage(%)
Class
Test 1
10
Class
Test 2
10
Home
Assignment
5
End
Attendance
Total
Term
Examination
5
70
100
Laboratory Assessment:
Components
(Drop down)
Weightage
(%)
Continuous Assessment/Internal
End Term Examination
Assessment
Class Test
Lab
Viva Attendance
Lab
Major
Minor
Viva
(Practical) Record
Record Experiment Experiment
(Practical)
/ Spotting
10
5
10
5
10
35
15
10
Total
100
Text / Reference Books:
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Basic Immunology. Basic Immunology, 4th Edition. Functions and Disorders of the Immune System. A. Abbas, A.H. Lichtman & S. Pillai. Elsevier
Saunders. 2012. ISBN-13: 978-1455707072
Kuby Immunology, 7th Edition. Judy Owen, Jenni Punt, Sharon Stranford. W.H. Freeman. 2013
Tumor Immunology and Immunotherapy Edited by. ISBN: 9781464137846.

Cancer Immunology and Immunotherapy. In Series “Current Topics in Microbiology and Immunology” Vol. 344. Springer-Verlag Berlin Heidelberg.
2011. 978-3-642-26788-8. Robert C. Rees. Oxford University Press. 2014. ISBN-13: 978-0199676866
Other Study Material:

Research Papers and Review Articles Published in Peer-Reviewed Scientific Journals.
Remarks and Suggestions:
Date: 11/09/2015