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Krakowski 1 Lane Tech College Prep High School LECTURE 2: PreCambrian: Origin of Life ORIGIN OF EARTH REVIEW: 1. Universe is 13.7 Billion Years Old 2. Solar System formed 4.6 BYA Most likely Scenerio for formation: Most matter in universe is light elements (Hydrogen) Heavy Elements (Carbon, Nitrogen, Oxygen) are formed only in core of dying stars Nearby Star Exploded Shockwave through Gas/Dust Cloud caused lumps to form Lumps Gravitationally move matter to themselves and cloud collapsed upon itself 99.9% of matter formed into the sun, the rest formed bodies such as planets, comets, etc. o The early Earth was a very different place: atmosphere :mostly carbon dioxide, nitrogen, and water vapor Earth was bombarded with asteroids and meteors Volcanic Eruptions formed first continents 4.1 BYA Oceans formed when Earth cooled 4.1 BYA Late Bombardment till about 3.9 BYA What is the Oldest Fossil of Life on Earth? o o How did the First Life Form Come to Exist? o o Spontaneous generation theory accepted until 1862 ________________________ (“life creation”) was a mystery… What is Spontaneous Generation? o First Life Fossils: __________________________ ~3.5 BYA Filamentous Strings of up to 40 cells Prokaryotes: _____________________________ organisms These prokaryotes began to __________________________ Produced oxygen Living things could arise from ____________________________things Examples: Francesco Redi’s Experiment fly maggots life arises from life NOT air Lazzaro Spallanzani’s experiment microorganisms arise from others not AIR Louis Pasteur’s experiment microorganisms arise from others not AIR How did First Life Form? o o Conditions on early Earth made the Origin of Life Possible In a sequence of stages about 3.8 BYA: 1. Abiotic synthesis of small organic molecules (inorganic _______________) 2. Joining of these small molecules into polymers (monomers ______________) 3. Packaging of molecules into “protobionts” (“precells/protocells”) 4. Origin of self-replicating molecules Lane Tech College Prep High School How did Step 1 Abiotic Synthesis occur? o o Hypothesis 1: 1920’s Oparin and Haldane (primordial soup) atmosphere = HIGH energy levels contributed to SPONTANEOUS chemical evolution Suggested early atmosphere= NH3, H2, CH3, some H20 Energy was supplied by _________________________________________ 1st organic (“life”) compounds may have been synthesized near submerged volcanoes and deep-sea vents o Hypothesis 2: Some organic compounds may have come from ____________________________ Carbon compounds have been found in some ___________________________ that landed on Earth90 Amino Acids have been found in meteors How did Step 2: Polymer Formation occur? Stanley Miller: Graduate student at UIC working with Harold Urey Build Apparatus to Test __________________________________Synthesis Simulated Early conditions on Earth Electrical Energy provided by spark discharge They found Amino Acids, Nucleic Acids, Fatty Acids, and other Organic Molecules! Repeated many times under many conditions (___________________- simulating the sun) Demonstrated that Oparin/Haldane Hypothesis _______________________________ Nobel Prize for Miller and Urey Did Extra-Terrestrial Sources Bring Complex Organic Molecules to Earth? o o o o Small organic (carbon) molecules polymerize when they are concentrated on hot sand, clay, or rock These molecule form polymers (_______________) of huge life molecules (biomolecules) Miller-Urey Experiment What was the Miller-Urey Experiment? o o o o o o o 2 Hypothesis: 1. Oparin/Haldane Hypothesis ___________________________- Soup 2. _____________________________ Source of Complex Organic Molecules STEP 1: CHEMICAL EVOLUTION o o o Krakowski 2 Miller/Urey Experiment will ___________________________if Oxygen is present ______________________________ probably were an important second source of organic compounds Comets and Meteorites are rich in organic compounds formed abiotically in deep space (water, ammonia, 74 amino acids) Recent experiments show that these molecules would survive impact! Step 3: Packaging of molecules into “(“precells/protocells”) How did this Occur? o o o o 1. Organic Molecules Accumulate 2. Polypeptides Form 3. Some assemblages were capable of carrying out primitive living reactions 4. Conversion of pre-cellular assemblages into organized cells enclosed by outer boundary membranes Lane Tech College Prep High School What are the Hypothesis for Protocell Formation? o o o 1. 2. 3. 4. 5. 6. Prokaryotic ______________________!!!!! use chemicals to make their OWN food/energy Cyanobacteria (Prokaryotic __________________________) evolve pump O2 in air (around 3.5 bya) Oxygen (O2) in atmosphere results in EARLY mass extinctions b/c it KILLS most life Prokaryotic __________________________________ (“O2 loving”) survive Cellular Respiration evolves Single Celled Eukaryotic organisms (around 2.1 BYA) Made by Endosymbiosis 7. Multicellular eukaroytic organisms Photosynthesis and the Oxygen Revolution o RNA! Trapped inside a “protocell” by chance about 4.0 BYA RNA came 1st b/c has the following properties: _____________________________!!! Less complex/less stable than DNA RNA into protein more direct than DNA into Protein Has 3 different phenotypes (characteristics), can fold into diverse shapes RIBOZYMES: ________________________ that can carry out reactions RNA can self assemble from individual nucleotides (polymerize easy into 1 strand) SHORT STRANDS OF RNA CAN REPLICATE Which Came First Autotrophs or Heterotrophs? o o o o o o Protocells – _______________________molecules surrounded by membrane - like structures Some properties associated with life Simple reproduction and metabolism Maintenance of internal environment Has membrane All were spontaneously FORMED by chance! Step 4: Which Came First RNA or DNA? o 2 Hypothesis Lipid Membrane Hypothesis ___________________________- lipids(fatty acids) that form vesicles o Coacervates- combo’s of proteins, lipid membranes and carbs Iron-Sulfide Chimney Hypothesis When hot iron sulfide in deep ocean vents, meets the cooler ocean, some of the mineral forms into chimneys These chimneys acted as ________________________________________ What were the First Cells? o Krakowski 3 Oxygenic photosynthesis probably evolved about 3.5 BYA in _______________________ A.k.a- Blue/green algae in oceans They made _____________________________________(fossils) What were the Effects of O2 accumulation in atmosphere about 2.7 billion years ago? o o Formed _________________________________ (reduced UV, warmed Earth) ~2.5 bya _________________________________ by UV radiation = ozone Protected from _________________________rays and provides _______________ Provided opportunity to gain energy from light Allowed organisms to exploit new ecosystems Lane Tech College Prep High School What were the First Eukaryotes? o o o o Single cell Multicellular eukaryotes appeared ~1.5 bya Oldest known fossils of eukaryotes relatively small algae (~1.2 bya) What were the Earliest Multicellular Eukaryotic Animals (METAZOA)? o ______________________________in inner membrane structures and functions Both have their own circular ___________________________ Both have their own ___________________________ What were the First Multicellular Organisms? o o The oldest fossils of eukaryotic cells date back 2.1 billion years ___________________________________ eukaryotics The theory of endosymbiosis proposes that _____________________________________ and CHLOROPLASTS were formerly small, free-living prokaryotes that were ________________________ by larger host cells Oldest known fossils of eukaryotes relatively small algae (~1.2 BYA) What Evidence Supports Endosymbiosis? o Krakowski 4 600 MYA _______________ then ___________________________ then _______________________ Marine and ________________________________________ What was the Ancestor of Metazoa? o Choanoflagellates based on _____________________________ and ______________________________________ Protists that form spherical colonies