Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download General Lab Solutions
Document related concepts
Transcript
Formula for diluting: C1xV1=C2xV2 1X PBS: 50mL 20X PBS stock add dH2O to make 1 L Autoclave on liquid cycle for 30 minutes 20X TBST: 1L 200mL 1M Tris-HCl (pH 8) 600mL 5M NaCl 10mL Tween 20 190mL dH2O 500mL 100mL Tris-HCl (pH 8) 300mL 5M NaCl 5mL Tween 20 95mL dH2O 50X Blot Buffer: 42g NaHCO3 16g Na2CO3 dH2O to 1 L 10X Running Buffer: 30g Tris 144g Glycine 5g SDS (****Be careful with SDS****) dH2O to 800mL pH to 8.3 dH2O to 1 L Coomassie Stain: 50mL Methanol 10mL Glacial Acetic Acid 40mL dH2O 0.25g Coomassie Brilliant Blue Fix/Destain for Coomassie: 250mL Methanol 50mL Glacial Acetic Acid 200mL dH2O 20X PBS: 160g NaCl 4.02g KCl 4g KH2PO4 18.46g Na2HPO4 dH2O to 1 L pH to 7.5 w/ HCl autoclave on liquid cycle for 30 minutes Tris-HCl, pH 8 (1M, 1L): 121.14g Tris dH2O to 800mL pH to 8 with HCl dH2O to 1L 20X SSC: 175g NaCl 88g Sodium Citrate dH2O to 800mL pH to 7.0 with HCl dH2O to 1 L 10X TBE: 108g Tris 55g Boric Acid 40mL 0.5M EDTA (pH 8) dH2O to 1 L LB Broth: 25g LB Broth mix dH2O to 1L Autoclave on Liquid for 30 minutes LB-Amp Plates: Add 30.5g/L of distilled water Autoclave in flask(s) w/ stir bar for 30 minutes (liquid cycle) Spin on stir plate until 55 degrees Celsius Add 1ml Ampicillin (100mg/ml) for every liter of media Pour into 10cm plates Transfer plates from RT to cold room after 24 hours. 50X TAE: 242g Tris 57.1ml Glacial Acetic acid 100ml 0.5M EDTA (pH 8) OR 37.2g Na2EDTA.2H2O (in “E” section of chemicals) Bring to 1L with dH2O 10X MMR (NOT for Transgenics): 58.4g NaCl 1.49g KCl 1.2g MgSO4 2.94g CaCl2 11.9g HEPES dH2O to 800 ml pH to 7.4 w/ NaOH dH2O to 1L
