Download Week 11 - Cellular Respiration - CMS

BIOLOGY 120 LAB
Exercise 9
CERRITOS COLLEGE
L. L. Harris
CELLULAR RESPIRATION
(rev. 12.08.01)
A. INTRODUCTION
Maintenance of all living things calls for a continuous supply and expenditure of energy. Energy is required to
maintain organization and, thereby, resist entropy.
What is the original source of this energy (most)? _________________________________________________
What process captures this energy? ___________________________________________________________
How is this energy transferred from one organism to another? _______________________________________
How does an organism release the energy? _____________________________________________________
Cellular Respiration – _______________________________________________________________________
________________________________________________________________________________________
Glycolysis – ______________________________________________________________________________
Anaerobic Respiration –_____________________________________________________________________
Animals & Some Bacteria – _____________________________________________________________
Yeast – _____________________________________________________________________________
Aerobic Respiration – _______________________________________________________________________
AEROBIC RESPIRATION CAN BE VIEWED AS THE "UNDOING" OF PHOTOSYNTHESIS:
CO2 + H2O --------------Light Energy---------------> GLUCOSE + O2
= PHOTOSYNTHESIS
GLUCOSE + O2 ----------------------------------------> CO2 + H2O + ATP
= AEROBIC RESPIRATION
Summary:
Glucose  Pyruvate + O2  ___________________________________________________
Glucose  Pyruvate + No O2  ___________________________________________________
(Animals/Bacteria)
Glucose  Pyruvate + No O2  ___________________________________________________
(Yeast)
ADDITIONAL VOCABULARY:
ECTOTHERM – _______________________________________________________________________
______________________________________________________________________________
ENDOTHERM – _______________________________________________________________________
______________________________________________________________________________
B. DEMONSTRATION OF AEROBIC RESPIRATION BY LIVING PLANT TISSUES
OBSERVE THE DEMO BEGUN BY YOUR INSTRUCTOR AT THE BEGINNING OF TODAY'S LAB.
GLUCOSE + O2 -----------------------> CO2 + H2O + ENERGY
CO2 + H2O <------------> H2CO3 <------------> H+ + HCO3
Bromthymol Blue – _________________________________________________________________________
+ Test For Carbon Dioxide – ________________________________________________________________
− Test For Carbon Dioxide – _________________________________________________________________
C. ANAEROBIC RESPIRATION BY YEAST CELLS
OBSERVE THE DEMO BEGUN BY YOUR INSTRUCTOR AT THE BEGINNING OF TODAY'S LAB.
D. DETERMINING WHETHER OR NOT OXYGEN GAS IS CONSUMED BY RESPIRING TISSUES.
The class will perform an investigation of cellular respiration by a living animal (chick or mouse). Follow
the instructions in the lab manual. Be able to answer the problems in the lab manual and on this
worksheet. Stick around we will discuss answers at lab’s end.
Respirometer – ____________________________________________________________________________
Why use Soda Lime? – ______________________________________________________________________
Why use Drierite? – ________________________________________________________________________
What is your data? – ________________________________________________________________________
We will not run an experimental control chamber along side the experimental chamber -- but if we did, what
would be the description of the control chamber? Hint: consider the title of this experiment (see above).
____________________________________________________________________________________
____________________________________________________________________________________
How is Metabolic Rate calculated? – ___________________________________________________________
Why is Metabolic Rate expressed as it is? (Metabolic Rate = ml O2 / [gram wgt x unit time]) ______________
____________________________________________________________________________________
HOME ASSIGNMENT:
M.R. COMPARISON FOR ENDOTHERMS VS. ECTOTHERMS
Endotherm data is from today’s exercise and the manual gives data for an ectotherm (frog). Be able to
discuss the following: A) Why is more costly, in terms of food, to be an endotherm? B) What is the
advantage offered the Endotherm? C) Could there be possible advantages to being an Ectotherm?