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The Famous Pea Experiment Mendel’s results depended on a lot of peas—and a lot of patience Prior to Mendel, naturalists and scientists tried to understand heredity by crossbreeding different varieties of plants or animals. Nevertheless, Mendel was the first to conduct broad, thorough, systematic, and sufficiently rigorous experiments to discern any universal laws governing inheritance. Mendel began by identifying seven pairs of contrasting traits found among garden peas: Seed color (yellow or green) Seed shape (smooth or wrinkled) Pod color (yellow or green) Pod shape (inflated or pinched) Flower color (purple or white) Flower position (axial or terminal) Stem height (tall or short) For two years, the scientist grew different varieties of peas to make sure that their offspring were always the same. Then be began breeding different varieties together to make hybrids. He brushed the pollen off yellow pea plants and put it on green pea plants, and did the same for plants with each of the seven pairs of traits. He then grew generation after generation of hybrids and tracked the inheritance of the traits. Mendel noticed that some traits disappeared in the first generation of hybrids. He called these traits “recessive.” He called those that did appear “dominant.” In later generations the recessive traits reappeared—and in a mathematically predictable pattern. For example, later generations of plants had one green pea for every three yellow peas. The same ratio appeared for all seven pairs of traits. Mendel grew an estimated 28,000 pea plants over eight years. In 1864 he published the results of his experiment.Mendel picked common garden pea plants for the focus of his research because they can be grown easily in large numbers and their reproduction can be manipulated. Pea plants have both male and female reproductive organs. As a result, they can either self-pollinate themselves or cross-pollinate with another plant. In his experiments, Mendel was able to selectively cross-pollinate purebred plants with particular traits and observe the outcome over many generations. This was the basis for his conclusions about the nature of genetic inheritance. STOP What Mendel Discovered Mendel’s mathematical mind allowed him to “see” hidden patterns of heredity. Earlier scientists had noticed the disappearance and reappearance of traits in hybrid plants. What Mendel did differently was count. And count. And count. Mendel used mathematics to draw conclusions about what was happening deep inside the cell. Mendel concluded that every trait must be controlled by two “elements” (what we now call genes) that are present in every pea plant. As part of sexual reproduction, these elements separate and only one is passed down to the offspring. Whether a plant has green or yellow peas depends on the combination of elements that it received from its parents. If a plant receives two dominant elements, its trait will be dominant (i.e. yellow peas). If it receives two recessive elements, its trait will be recessive (i.e. green peas). If it receives one of each element, the dominant trait will mask the recessive trait. This is what caused all the green peas to disappear in Mendel’s first generation of plants. Mendel went on to cross plants that differed in more than one trait—round-yellow peas with wrinkled-green ones, or tall, violet-blossomed plants with short, white-blossomed ones. As in his initial experiments, the traits appeared in predictable ratios. This told Mendel that the elements governing traits were not linked, but passed separately to the offspring. Mendel’s results were published in a local scientific journal in 1866, but other scientists did not understand the importance of his work for several decades. Meet Modern Mendels Mendel’s scientific “heirs” use modern techniques to broaden our understanding of heredity. While Mendel would probably be amazed by the technology used by his modern counterparts, he would find the questions they are investigating familiar. Scientists today use DNA to explore evolution, conservation and other aspects of the natural world. For example: Mendelian genetics helps fill the branches on the Darwinian “Tree of Life.” The “Early Bird” project, led by Field Museum scientist Dr. Shannon Hackett aims to chart genetic links among major groups of birds. DNA can reveal things about an animal’s behavior that binoculars can’t. Field Museum scientist Dr. Kevin Feldheim samples DNA from lemon sharks in the Bahamas for a University of Illinois-Field Museum study of reproductive patterns— information that is crucial to protect these threatened creatures. DNA proves that Neanderthals and modern humans are not even distant relatives. In 1997 Svante Pääbo and a team of German and American scientists were the first to extract DNA from Neanderthals. Their research indicates that it has been more than 600,000 years since Neanderthal and Homo sapiens shared a common ancestor. Today’s plant geneticists study “gene flow.” Sonal Singhal, a student at Washington University in St. Louis, studies the exchange of genes between domesticated rice and its wild ancestor. “Gene flow” can produce weedy, invasive hybrids that interfere with cultivation and reduce crop yield.