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HONORS BIOLOGY STUDY GUIDE CHAPTER 10 Name______________________ Date___________ Per _______ RACE TO FIND THE STRUCTURE OF DNA: Read 10.1-10.3 Match the DNA Scientists pp. 182-187 A. Watson and Crick C. Rosalind Franklin B. Hershey and Chase D. James Chargaff _____used radioactive DNA and protein to find DNA =genetic material in T2 bacteriophages _____found A = T and C = G amounts in a DNA strand _____won Nobel Prize for finding the structure of DNA _____used X-ray crystallography to show the shape of DNA molecule READ 10.2 DNA and RNA are polymers of nucleotides pp. 186-7 1. What does DNA stand for? 2. Label the complementary base pairs. (A, T, C, G) 3. Label the phosphates. (P) 4. Label the hydrogen bonds. (H) 5. Label the deoxyribose sugars. (S) 6. Circle ONE nucleotide. 7. How do the opposite strands of the DNA molecule relate to each other? 8. What do the nitrogenous base letters stand for: A____________________ C__________________ T____________________ G__________________ 9. Which of the above are purines?______________________ Which of the above are pyrimidines?_____________________ Which are single ringed?__________________________ Which are double ringed?__________________________ 10. What does RNA stand for?_________________________________ How is RNA different from DNA in: sugar_________________ a base____________________________ 11. What functional group(s) is (are) in nitrogenous bases? Honors Biology Study Guide 10 p. 2 READ 10.4-5 p.188189 1. What does it mean that DNA replication is semi-conservative? Antiparallel? 2. Sketch replication bubbles of two daughter DNA molecules. 2. Fill in the missing bases to show DNA replication: Read 10.6-14 THE CENTRAL DOGMA pp. 190-197 1. Match structures: A-DNA D-ribosomal units B-mature mRNA E-protein C-Amino acid F-pre-mRNA Match processes: transcription (P) translation NUCLEUS G- anticodon H-tRNA I-codon Processes Honors Biology Study Guide 10 p. 3 3. What is the mRNA strand that would be copied from this DNA strand? G G C T A T A T C C T G C G C T A T A C G C T A ______ _____ ______ ______ _____ ______ _____ ______ 4. MATCH THE TERMS WITH THEIR DEFINITIONS: _____introns A. protein + this make up a ribosome subunit _____exons B. RNA protein _____tRNA D. DNA mRNA _____mRNA E. organelles that position tRNA and mRNA to make proteins _____rRNA F. triplet loop of bases on tRNA _____codon G. triplet of bases on mRNA _____anti-codon H. non-coding regions of RNA that are removed _____RNA polymerase I. coding regions of RNA that are expressed and leave nucleus _____ribosomes J. transcription enzyme _____transcription K. type of RNA used in translation (convert codons to protein) _____translation L. type of RNA to code from DNA to take code out of nucleus READ 10.7-8 Amino Acid Sequences 1. How many amino acids are common to all organisms?_______ 2. What do we call the length of three DNA bases?__________ 3. Looking at the following codon chart, write the amino acids that these codons would form: AUC___________________ AUG___________________ CAC___________________ What three codons would stop translation? READ 10.9 p. 193 TRANSCRIPTION Fill in the blanks by looking at Fig. 10.5 1. _______________________is the enzyme that adds RNA nucleotides. 2. _______________________is the start transcribing signal nucleotide sequence. 2. _______________________is the attachment of RNA polymerase to the promoter. 3. _______________________is when the RNA grows longer. 4._______________________is the DNA template when the RNA polymerase reaches a pointto stop. Biology Study Guide 10 p. 4 READ 10.10-12 p.194-6 1. What is the job of mRNA? 2. Not all the mRNA leaves the nucleus. What is the part of mRNA called that are noncoding and remain in the nucleus?_________________________What is the part of mRNA that is expressed and leaves the nucleus to travel to the ribosomes?______________________ 3.What type of RNA converts nucleic acid language (codons) to amino acid language?_______ 4. Identify on this tRNA where the anticodon would be and where the amino acid acceptor would be. 10.13-14 Read pp. 196-7 What happens at each site of translation at the ribosome: A site- P site- E site- What is translocation? READ 10.16 Mutations p199 Matching: _____silent mutation _____nonsense mutation _____missense mutation _____Reading frame _____mutation _____mutagens A. any change in the DNA sequence B. triplet grouping of nucleotides C. any physical or chemical agent causing mutations D. if no change in the protein product from a base sequence change E. a mutation does cause a change in the protein coded F. substitutions of bases that lead to a stop codon Biology Study Guide 10 p. 5 READ 10.17 Viral DNA may become part of the host chromosome p. 200 1. Why is a virus called “genes in a box?” (make a sketch) 2. FILL IN THE CHART OF THE COMPARISON OF THE TWO VIRUS CYCLES: Description LYTIC The virus lands on the cell and injects its ________. New phage _______ and ___________are synthesized. The phages then__________________. The cell _______________, releasing phages. LYSOGENIC Sketches Viral DNA is inserted into:______________________ The host cell replicates the _______________ DNA along with its own DNA. The prophages may remain in the host cell _______________. Occasionally something triggers the ____________ of the prophage from its host. The freed DNA now goes into the _________ cycle. READ 10.22-23 BACTERIA pp. 204-5 1. Most bacteria has a single chromosome in what shape?________ MATCH: _____DNA transfer between two bacteria _____uptake of DNA from the surrounding environment _____small, circular DNA separate from the bacteria regular chromosome _____plasmid that are resistant to antibiotics _____piece of DNA that makes sex pili and an origin of replication _____physical union of bacteria and transferring DNA . F factor B. R plasmid C. plasmid D. transformation E. transduction F. conjugation