Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
FIRST SEMESTER – APRIL 2014
BT 1828 - IMMUNOLOGY & IMMUNOTECHNOLOGY
Date : 07/04/2014
Time : 09:00-12:00
Dept. No.
Max. : 100 Marks
PART – A
Answer all the questions
(20 marks)
I. Choose the correct answer:
(5×1 = 5 marks)
(1) Which one of the following is not an antigen presenting cell?
(a) B cell (b) Macrophage (c) Mast cell (d) Dendritic cell
(2) Lipid antigens are presented to T cells by
(a) MHC I
(b) MHC II
(c) CD1
(d) CD 28
(3) Tissue typing can be done using
(a) Immunodiffusion (b) Mixed lymphocyte reaction (c) Agglutination (d) Elispot assay
(4) A myeloma cell used for hybridoma technique should be
(a) DHFR-negative (b) Antibiotic resistant (c) HGPRT-negative (d) HAT-resistant
(5) Which one of the following technique is used for counting different types of cells?
(a) Flow cytometry
(b) Immunochromatography
(c) Western blotting
(d) Agglutination
II. State whether the following statements are true or false, if false give reason: (5×1= 5 marks)
(6) IL-1promotes T and B lymphocyte proliferation.
(7) An individual can inherit a maximum of 12 different MHC II molecules.
(8) Alpha fetoprotein is a tumour-associated antigen.
(9) Vector vaccines are live recombinant vaccines.
(10) Indirect ELISA can be used to detect antibodies against HIV in a serum sample.
III. Complete the following:
(5×1 = 5 marks)
(11) _______ is a secondary lymphoid organ.
(12) The human immunoglobulin heavy chain gene is present on chromosome ________.
(13) IgE have to bind to ________ cells to trigger Type I hypersensitivity.
(14) _______ vaccines consist of only the purified major antigenic determinant.
(15) _________ membrane is used for immunoscreening of recombinant libraries.
IV. Answer the following, each within 50 words only
(5×1 = 5 marks)
(16) What are Peyer’s patches?
(17) Name any two types of macrophages.
(18) Explain how cancer immunotherapy can be done to increase antigen presenting cell activity.
(19) Give any two reasons for the low immune response to P. falciparum infection.
(20) What is meant by immune panning?
PART – B
Answer the following, each in about 500 words only.
Draw diagrams wherever necessary.
(5×8 = 40 marks)
21(a) Explain the structure and function of the spleen.
OR
(b) Describe the different cells of the immune system.
22(a) Describe the immune responses mounted against bacteria and bacterial evasion mechanisms.
OR
(b) Give an account of the immune response to viruses and viral evasion of the immune system.
23(a) Discuss the methods of tissue typing for organ transplantation.
OR
(b) Write notes on:
(i) Oncogenes and the function of oncogenic proteins
(ii) Types of transplants
24(a) Write notes on the production of a peptide vaccine for V. cholerae.
OR
(b) Explain the following:
(i) Conventional vaccines
(6)
(ii) Tamiflu
(2)
25(a) Describe the technique of immuno-PCR.
OR
(b) How can cells be sorted using magnetic sorting techniques?
PART – C
Answer any two of the following, each within 1500 words;
Draw diagrams wherever necessary.
26. Explain the types, mechanism of induction and treatment of autoimmunity.
27. Discuss the different pathways of complement fixation.
28. Describe the following:
(i) Antibody engineering in E.coli
(ii) Immunoscreening of recombinant libraries
29. Write notes on the production and applications of monoclonal antibodies.
(2×20 = 40 marks)