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Acta Scientiarum. Technology ISSN: 1806-2563 [email protected] Universidade Estadual de Maringá Brasil Navarro da Silva, Adriana; Garcia-Cruz, Crispin Humberto A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii Acta Scientiarum. Technology, vol. 32, núm. 2, 2010, pp. 105-112 Universidade Estadual de Maringá Maringá, Brasil Available in: http://www.redalyc.org/articulo.oa?id=303226526006 Abstract Alginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 26-2 factorial design. The second was based on significant variables of the first, resulting in a full 33-0 factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g-1 cell h-1 and alginate from 100 to 1,600 mg g-1 of cell h-1. The productivity of both compounds was in the maximum incubation temperature of 62°C, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L-1. In both experiments the PHB extracted presented purity of 94%. Keywords Alginate, polyhydroxybutyrate (PHB), production, Azotobacter vinelandii. How to cite Complete issue More information about this article Journal's homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative
