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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY FIRST SEMESTER – NOVEMBER 2007 AE 15 BT 1807 - MICROBIOLOGY Date : 25/10/2007 Time : 1:00 - 4:00 Dept. No. Max. : 100 Marks PART A Answer all the Questions (20 Marks) I. Choose the correct answer. (5 x 1 = 5) 1. Statement A: Disinfection kills all forms of microbial life. Statement B: It is mainly applied to living tissues. (a) Statement A is correct, but statement B is incorrect. (b) Statement B is correct, but statement A is incorrect. (c) Both A and B are correct. (d) Both A and B are incorrect. 2. The instrument used in tyndallization (a) Autoclave (b) Tyndallizer (c) Arnold sterilizer (d) Gamma sterilizer. 3. Asymbiotic Nitrogen fixation was first recognized in (a) Azotobacter sp. (b) Clostridium botulinum (c) Rhizobium sp. (d) Clostridium pasteurianum. 4. Bacteria that is capable of oxidizing sulphur to sulphates is (a) Sulfobacterium (b) Methanobacterium (c) Acetobacter (d) Thiobacillus. 5. Chemotrophs obtain energy from (a) sunlight (b) H- donor (c) chemicals (d) carbon. II. State True or False; if false state the reason. (5x1=5) 6. β-carotene from Spirulina acts as an anti-cancerous agent. 7. Influenza virus is an example of enveloped helical virus. 8. Phosphorus is most commonly found as apatite in rock sediments. 9. Denitrification is the release of ammonia from nitrate. 10. Streptomycin is commercially produced as hydrochlorides. III. Complete the following: (5x1=5) 11. The term ‘antibiotic was coined by----------------------------------12. The bacteria that is devoid of cell wall is --------------------------13. --------------------------- causes crown gall disease in plants. 14. Thermolabile substances can be sterilized by -----------------------. 15. Many peptide vaccines, chemically linked together using polylysine is called ------------------------------IV. Answer the following questions each in about 50 words: (5x1=5) 16. What is humus? 17. What are sub-unit vaccines? 18. What are capnophiles? 19. Give short notes on Commensalism. 1 20.Define neurotoxin. PART B V. Answer any five of the following questions, each in about 350 words. (5x8=40) 21. Give a general account on viral structure, and replication. 22. Brief out the various methods of culture preservation. 23. Describe the biogeochemical cycling of Carbon. 24. Explain the industrial applications of Saccharomyces spp. 25. Explain briefly the environmental conditions required for the growth of bacteria. 26. Illustrate the procedure for the vinegar biosynthesis. 27. Brief out the life cycle of Neurospora. 28. Give a general account on the population interactions. PART C VI. Answer the following questions in detail, each in about 1200 words. (2x20=40) 29. (a) What is sterilization? Explain the physical methods of sterilization in detail. (or) (b) Describe the eucaryotic cell structure and function. 30. (a) Explain the mechanism of Symbiotic Nitrogen fixation. (or) (b) Discuss briefly the production process of bacterial and algal single- cell protein. ************** 2