Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – APRIL 2012
BT 1819 - MICROBIAL PHYSIOLOGY & GENETICS
Date : 17-04-2012
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART-A (20 marks)
Answer ALL questions
I Choose the correct answer:
(5 x 1 = 5marks)
1. Match
A) Jenner
B) Lister
C) Ehrlich
D) Metchnikoff
with i) phagocytosis ii) vaccination iii) antiseptic surgery iv) chemotherapy
a) A-ii, B-iv, C-iii, D-i
c) A-ii, B-iv, C-i, D-iii
b) A-ii, B-i, C-iii, D-iv
d) A-ii, B-iii, C-iv, D-i
2. _____________utilize solar radiation as a source of energy.
a) Heterotrophs
b) Phototrophs
c) Mixotrophs
d) Chemotrophs
3. F plasmid is made up of _________ and ________ locus.
a) tra & trb
b) trb & trc
c) trc & trd
d) trd & tre
4. ____________ gene recognizes unmethylated GATC during transition period.
a) MutH
b) MutL
c) MutI
d) MutS
5. Statement A: Clostridium botulinum is responsible for food poisoning called botulism.
Statement B: It is a Gram-negative, aerobic spore forming bacterium.
a) Statement A is correct, Statement B is incorrect
b) Statement B is correct, Statement A is incorrect
c) Both A and B are correct
d) Both A and B are incorrect
II State whether the following statements are True or False; if false give reason:
(5 x 1 = 5 marks)
6. Antiseptic is a chemical, usually applied on living tissues.
7. Phycocyanin is present in red algae.
8. Colicin is used to kill closely related strains of the bacteria ColE1.
9. UV radiation causes breaking of phosphate ester linkages.
10. Nitrification is the process of breaking down of nitrates to free atmospheric nitrogen.
III Complete the following:
(5 x 1 = 5marks)
11. If bacteria divide in one plane and remain as a chain, it is said to be ___________ and an
example is _________________.
12. Phosphoglycerate kinase phosphorylates 3-phosphoglycerate to produce ____________ .
13. ‘a’ cell of yeast produces _____________ which signals the presence of ‘a’ cell.
14. ______________ is an example of a chemical mutagen.
15. The study of microbes occurring in water is termed as _______ _____________.
IV Answer the following, each in about 50 words:
(5 x 1 = 5 marks)
16. What is a mesosome? Mention its function.
17. Define methanogenesis.
18. What is bacterial transduction?
19. Define SOS repair.
20. Distinguish between transient and resident flora.
PART-B
Answer the following, each in about 500 words; Draw diagrams wherever
necessary
(5 x 8 = 40 marks)
21. (a) Give the salient features of Whittaker’s five kingdom concept. Why are viruses not
included in this system of classification?
OR
(b) Draw a neat diagram of Gram-negative bacterial cell wall. Mention any three parts and
their functions.
22. (a) Explain the role of bacteriorhodopsin and phycobiliproteins during metabolism.
OR
(b) Briefly explain leghemoglobin and nif gene regulation.
23. (a) Give an account of bacterial conjugation.
OR
(b) Write notes on hydrogen iron and sulphur oxidation.
24. (a) Write an account on the trp operon.
OR
(b) What is the origin of mutation? Explain.
25. (a) Give an account on nitrogen cycle, with a note on the role of microbes.
OR
(b) Expand the following, each with a brief note:
i) VAM
ii) DMC
iii) SCP
iv) HIV
PART-C
Answer any TWO of the following, each within 1500 words; Draw diagrams wherever
necessary
(2 x 20 = 40 marks)
26. Give an account on any four physical agents used in the control of microbes.
27. Explain light and dark reactions in photosynthesis.
28. What is a bacteriophage? Explain the lytic and lysogenic cycle in detail.
29. Discuss the causal organism, symptoms and treatment of any one bacterial disease
studied by you.
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