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DE–2049 11 DISTANCE EDUCATION M.Phil. (Zoology) DEGREE EXAMINATION, MAY 2011. (Non-Semester) RESEARCH METHODOLOGY AND LABORATORY TECHNIQUES (2006 Batch) Time : Three hours Maximum : 100 marks Answer any FIVE questions. All questions carry equal marks. (5 20 = 100) 1. Give an account on principal and applications of light microscopy and electron microscopy. 2. Discuss about the principle and operation method of pH meter and add a note on preparation of buffers. 3. Critically evaluate the followings : (a) Effect of Radioactive isotopes on biological system (b) Principles of liquid scintillation counter. (c) Application of biological systems. various radioactive isotopes (d) Application of autoradiography. 4. Discuss the principle, method and application of HPLC. 5. Give an account on immune diffusion and Hybridoma technology. 6. Write notes on different types of blotting techniques and their applications. 7. Comment on the following : (a) Gene and Genome (b) Protein and Proteomes (c) Drug designing (d) Genomics to proteomics. 8. Describe the principle and application of X-ray diffraction. —————— 1 DE–9064 in DE–2050 12 DISTANCE EDUCATION M.Phil. (Zoology) (Non-Semester) DEGREE EXAMINATION, MAY 2011. ANIMAL DIVERSITY, RESOURCES AND CONSERVATION (2006 Batch) Time : Three hours Maximum : 100 marks Answer any FIVE questions. (5 20 = 100) All questions carry equal marks. 1. Give an account on Numerical taxonomy. Explain the differences in cladistics and phenetics approaches in taxonomy. 2. Elaborate the diversity of non-chordates and chordates. 3. Explain the anthropogenic mediated threats to animal biodiversity and discuss the conservative measures to overcome introduces exotic species mediated biodiversity collapses. 4. What are the major levels of biodiversity classification, explain with suitable examples? 5. What are the stages involved in Environmental impact assessment (EIA)? 6. Explain the role of Global warming in climatic changes. How Global Warming affects the biodiversity of marine environment? 7. What are the renewable energy sources? Discuss the importance of nuclear energy in sustainable energy production. 8. What are the threats to biodiversity and discuss their impacts? ———————–- DE–2051 13 DISTANCE EDUCATION M.Phil. (Zoology) (Non-Semester) DEGREE EXAMINATION, MAY 2011. ANIMAL BIOTECHNOLOGY 2 DE–9064 (2006 Batch) Time : Three hours Maximum : 100 marks Answer any FIVE questions. (5 20 = 100) All questions carry equal marks. 1. Explain the applications of cryopreservation and list out its risk in cell culture. 2. Describe various methods used to measure cell death. 3. Write detail account on application of embryonic stem cell. 4. Write an essay on “Cell separation techniques”. 5. Write notes on the following : (a) Baculo virus (b) Vaccinia virus (c) Cell cloning (d) DNA microarray (e) Transgenics. 6. Explain in detail on Chemical and Biological methods of gene transfer. 7. Enumerate the application of Gene therapy. 8. Write an essay on “Applications of animal biotechnology in production of hormones and other therapeutic proteins”. ––––––––––––––– 3 DE–9064