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Principal Investigator/Program Director (Last, First, Middle): BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES. NAME POSITION TITLE Jihyeon Lim Assistant Professor eRA COMMONS USER NAME EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) INSTITUTION AND LOCATION Korea University Korea University University of Massachusetts at Amherst Albert Einstein College of Medicine DEGREE (if applicable) YEAR(s) BS MS PhD Postdoc 1991-1995 1995-1997 1999-2004 2004-2005 FIELD OF STUDY Chemistry Inorganic Chemistry Analytical Chemistry BioAnalytical Chemistry Positions and Honors 1995-1997 Research Assistant, Korea Basic Science Institute, Seoul branch, Korea 1997-1998 Research Scientist, Product Research Department, Hanwha Energy Co., Ltd., Daejon, South Korea 1999-2004 Research Assistant (Ph.D. Candidate), University of Massachusetts at Amherst, MA, NY 2004–2005 Research Associate, Department of Molecular Pharmacology, Albert Einstein College of Medicine at Bronx, NY 2005-2007 Associate, Laboratory for Macromolecular Analysis & Proteomics Albert Einstein College of Medicine at Bronx, NY 2007-present Assistant Professor, Department of Pathology, Albert Einstein College of Medicine at Bronx, NY Professional service 2006-present Reviewer, Analytical Biochemistry Memberships: American Chemical Society, American Society for Mass Spectrometry Publications 1. Du, P. C.; Stolovitzky, G.; Horvatovich, P.; Bischoff, R.; Lim, J.; Suits, F. “A noise model for mass spectrometry based proteomics” Bioinformatics 2008, 24 (8), 1070-1077. 2. Che, F.Y; Zhang, X.; Berezniuk I.; Callaway, M.; Lim, J; Fricker, L.D. “Optimization of neuropeptide extraction from the mouse hypothalamus” J. Proteome Res. 2007, 6(12), 4667-4676. 3. Srikanth, R.; Wilson, J; Bridgewater, J. D.; Numbers, J. R.; Lim, J.; Olbris, M.R.; Kettani,A.; Vachet, R.W. “Improved Sequencing of Oxidized Cysteine and Methionine Containing Peptides Using Electron Transfer Dissociation” J. Am. Soc. Mass Spectrom. 2007, 18(8),1499-506. 4. Bridgewater, J.D.; Srikhanth, R.; Lim, J.; Vachet, R.W. “The Effect of Histidine Oxidation on the Dissociation Patterns of Peptide Ions” J. Am. Soc. Mass Spectrom. 2007, 18(3), 553-562. 5. Bridgewater, J.D.; Lim, J.; Vachet, R.W. “Using Metal-Catalyzed Oxidation Reactions and Mass Spectrometry to Identify Amino Acid Residues within 10Å of the Metal Center in Cu-Binding Proteins” J. Am. Soc. Mass Spectrom. 2006, 17(11), 1552-1559. 6. Bridgewater, J.D.; Lim, J.; Vachet, R.W. “Transition Metal-Peptide Binding Studied by Metal-Catalyzed Oxidation Reactions and Mass Spectrometry” Anal. Chem. 2006, 78(7), 2432-2438. 7. Lim, J.; Berezniuk, I.; Che, F.; Parikh, R.; Biswas, R.; Pan, H.; Fricker, L.D. “Altered Neuropeptide Processing in Prefrontal Cortex of Cpe (fat/fat) Mice: Implications for Neuropeptide Discovery” J. Neurochem. 2006, 96(4), 1169-81. PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page Biographical Sketch Format Page Principal Investigator/Program Director (Last, First, Middle): 8. Fricker, L.D.; Lim, J.; Pan, H.; Che, F. “Peptidomics: Identification and Quantification of Endogenous Peptides in Neuroendocrine Tissues” Mass Spectrom. Rev. 2006, 25(2), 327-44. 9. Che, F.; Lim, J.; Biswas, R.; Pan, H; Fricker, L.D. “Quantitative Neuropeptidomics of Microwaveirradiated Mouse Brain and Pituitary” Mol. Cell. Proteomics 2005, 4(9),1391-405. 10. Lim, J.; Vachet, R. W. “Using Mass Spectrometry To Study Copper-Protein Binding under Native and Non-Native Conditions: -2-Microglobulin” Anal. Chem. 2004, 76(13), 3498-3504. 11. Lim, J.; Vachet, R. W. “Development of a Methodology Based on Metal-Catalyzed Oxidation Reactions and Mass Spectrometry to Determine the Metal Binding Sites in Copper Metalloproteins” Anal. Chem. 2003, 75(5), 1164-1172. 12. Lim, J. H.; Park, T. H.; Lee, H. J.; Lee, K. B.; Jang, H. G. “A New Functional Model Complex of Extradiol-Cleaving Catechol Dioxygenases: Properties and Reactivity of [Fe-II(BLPA)DBCH] BPh4” B. KOR. CHEM. SOC. 1999, 20 (12), 1428-1432. 13. Lim, J. H.; Lee, H. J.; Lee, K. B.; Jang, H. G. “A New Functional Model of Catechol Dioxygenases: Properties and Reactivity of [Fe(BLPA)DBC]BPh4 ” B. KOR. CHEM. SOC. 1997, 18 (11), 1166-1172. Research Support Recently, Dr. Lim’s research is more focused on the cancer biomarker discovery. She is developing independent and significant predictive measures of tumor behavior for head and neck squamous cell carcinoma and hepatocellular carcinoma. Quantitative measurements of protein expression either in tissues or sera utilizing multidimensional separation and mass spectrometry have been major tools to achieve the goal. A few peptide markers, which distinguished different tumor behaviors, have been discovered successfully. The results will be integrated with the RNA expression data to identify the best potential diagnostic and prognostic biomarkers to develop clinical diagnostics as a part of the system biology approach. Dr. Lim’s skills in the mass spectrometry area have been directed toward neuropeptidomics. Neuropeptides influence a wide range of physiological processes including feeding and body weight regulation, circadian rhythms, sleep, pain, fear, anxiety, learning and memory, and reward mechanisms. Quantitative and qualitative analyses of the vast array of neuropeptides are important for understanding brain function. Many challenges, however, exist with their analysis, including low concentrations and difficulties in labeling them for accurate quantitation. She has developed novel labeling approaches to facilitate neuropeptide quantitation. This method has been utilized to find up- or down-regulated neuropeptides under different stimuli to help in understanding the function of different neuropeptide hormones. PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page Continuation Format Page