Download Gene Section EEF1A2 (eukaryotic translation elongation factor 1 alpha 2)

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Gene Section
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EEF1A2 (eukaryotic translation elongation factor 1
alpha 2)
Sanna Noubir, Jonathan M Lee
Department of Biochemistry, Microbiology, Immunology, University of Ottawa, 451 Smyth Road, Ottawa,
Ontario, Canada K1H 8M5. (SN, JML)
Published in Atlas Database: April 2008
Online updated version : http://AtlasGeneticsOncology.org/Genes/EEF1A2ID40408ch20q13.html
DOI: 10.4267/2042/44423
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence.
© 2009 Atlas of Genetics and Cytogenetics in Oncology and Haematology
Expression
Identity
In mice and humans, eEF1A2 is expressed only in
normal tissues of the brain, heart and skeletal muscle.
Expression is found in tumours of the breast, lung and
ovary.
Other names: EEF1AL; EF-1-alpha-2; EF1A; HS1;
STN; eEF1A-2
HGNC (Hugo): EEF1A2
Location: 20q13.3
Localisation
DNA/RNA
Diffusely cytoplasmic and nuclear.
Function
Description
This protein is an isoform of the alpha subunit of the
elongation factor-1 complex. There are two known
isoforms of protein elongation factor eEF1A (eEF1A1
and eEF1A2). eEF1A proteins are GTP-binding
proteins that interact with amino-acylated tRNA and
recruit them to the ribosome during the elongation
phase of protein translation. In addition, eEF1A2 binds
to and stimulates the lipid kinase activity of
phosphatidylinositol 4-kinase beta, the enzyme that
catalyzes the generation of phospha-tidylinositol 4phosphate from phosphatidylinositol.
8 exons and 7 introns.
Transcription
5 alternatives for transcript splicing.
Protein
Description
50kDa; 463 amino acids contains tRNA binding sites,
GTP binding and hydrolysis sites and putative actin
binding sites (see figure below).
Atlas Genet Cytogenet Oncol Haematol. 2009; 13(3)
196
EEF1A2 (eukaryotic translation elongation factor 1 alpha 2)
Noubir S, Lee JM
eEF1A2 also activates the Akt serine/threonine kinase
and can stimulate actin remodeling and increase the
rate of cell invasion and migration in vitro.
vitro and also enhances their tumori-genicity in mouse
xenograft models.
References
Homology
Anand N, Murthy S, Amann G, Wernick M, Porter LA, Cukier
IH, Collins C, Gray JW, Diebold J, Demetrick DJ, Lee JM.
Protein elongation factor EEF1A2 is a putative oncogene in
ovarian cancer. Nat Genet. 2002 Jul;31(3):301-5
EEF1A2 is highly homologous to the EEF1A1.
EEF1A2 and EEF1A1 share greater than 90% DNA
sequence and amino acid identity. The GTP binding
and hydrolysis domains also have homology to the Ras
GTPase.
Tomlinson VA, Newbery HJ, Wray NR, Jackson J, Larionov A,
Miller WR, Dixon JM, Abbott CM. Translation elongation factor
eEF1A2 is a potential oncoprotein that is overexpressed in
two-thirds of breast tumours. BMC Cancer. 2005 Sep 12;5:113
Mutations
Amiri A, Noei F, Jeganathan S, Kulkarni G, Pinke DE, Lee JM.
eEF1A2 activates Akt and stimulates Akt-dependent actin
remodeling, invasion and migration. Oncogene. 2007 May
10;26(21):3027-40
Note
Inactivation of mouse EEF1A2 leads to immunodeficiency, neuromuscular abnormalities and death by
30 days of age.
Jeganathan S, Lee JM. Binding of elongation factor eEF1A2 to
phosphatidylinositol 4-kinase beta stimulates lipid kinase
activity and phosphatidylinositol 4-phosphate generation. J Biol
Chem. 2007 Jan 5;282(1):372-80
Implicated in
Tumorigenesis
Kulkarni G, Turbin DA, Amiri A, Jeganathan S, AndradeNavarro MA, Wu TD, Huntsman DG, Lee JM. Expression of
protein elongation factor eEF1A2 predicts favorable outcome in
breast cancer. Breast Cancer Res Treat. 2007 Mar;102(1):3141
Disease
Breast, lung and ovarian cancer. EEF1A2 overexpression is found in approximatively 60% of breast,
30% of ovary and 40% of lung tumours.
Prognosis
High eEF1A2 protein expression is associated with
good prognosis in both breast and ovarian cancer. High
eEF1A2 protein expression predicts poor prognosis in
lung cancer.
Cytogenetics
Fluorescence in situ hybridization (FISH), showed high
copy numbers of EEF1A2 in 25% ovarian tumors.
Oncogenesis
The eEF1A2 gene is transforming and increases the invitro growth rate of mouse and human cells grown in-
Atlas Genet Cytogenet Oncol Haematol. 2009; 13(3)
Tomlinson VA, Newbery HJ, Bergmann JH, Boyd J, Scott D,
Wray NR, Sellar GC, Gabra H, Graham A, Williams AR, Abbott
CM. Expression of eEF1A2 is associated with clear cell
histology in ovarian carcinomas: overexpression of the gene is
not dependent on modifications at the EEF1A2 locus. Br J
Cancer. 2007 May 21;96(10):1613-20
Pinke DE, Kalloger SE, Francetic T, Huntsman DG, Lee JM.
The prognostic significance of elongation factor eEF1A2 in
ovarian cancer. Gynecol Oncol. 2008 Mar;108(3):561-8
This article should be referenced as such:
Noubir S, Lee JM. EEF1A2 (eukaryotic translation elongation
factor 1 alpha 2). Atlas Genet Cytogenet Oncol Haematol.
2009; 13(3):196-197.
197