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Using Punnett Squares Dominant & Recessive Punnett Squares It is a special chart, or grid system, named after its inventor Reginald Crundall Punnett. (devised in the early 1900’s) Punnett Squares Geneticists often use Punnett squares to depict genetic crosses and to determine the possible gamete combinations of the offspring. Punnett Squares Punnett square: a diagram used to visualize genetic crosses (a chart that shows all the possible combinations of alleles that can result from a genetic cross) Punnett Squares How to work Dominant and Recessive Genetic Problems with Punnett Squares Show a cross between a tall and short pea plant Step One Write down the set of contrasting traits. Assign the capitol letter to the dominant trait and the lower case letter to the recessive trait. Tall T Short t Show a cross between a tall and short pea plant Step Two Write down possible genotypes and phenotypes. TT Tall Tt Tall tt Short Show a cross between a tall and short pea plant Step Three Fill in the genotypes of the parents on the punnett square. Punnett Squares On the top- the possible gametes of the female are listed. Down the left sidethe possible gametes of the male are listed t T T t Show a cross between a tall and short pea plant Step Four Fill in the genetic possibilities of the F1 t T T t Show a cross between a tall and short pea plant Step Four Take first allele from the male and first allele from the female and place in the first square. t T Tt T t Show a cross between a tall and short pea plant Step Four Take first allel from the male and second allele from the female and place in the second square. t T T t Tt Show a cross between a tall and short pea plant Step Four Take second allele from the male and first allele from the female and place in the third square. t T T Tt t Show a cross between a tall and short pea plant Step Four Take second allele from the male and second allele from the female and place in the fourth square. t t T T Tt Show a cross between a tall and short pea plant Step Four Fill in the genetic possibilities of the F1 t t T Tt Tt T Tt Tt Show a cross between a tall and short pea plant Step Five Write down the genotypic ratio and the phenotypic ratio. Purebred dominant : Hybrid : Purebred recessive Tall : Short Show a cross between a tall and short pea plant Step Five Write down the genotypic ratio and the phenotypic ratio. t t T Tt Tt T Tt Tt 0:4:0 4:0 Punnett Squares The gametes are then combined in each of the boxes within the square to give the possible gamete combinations of the offspring. F f F FF F f f Ff f f 1:2:1 3:1 F1 Punnett Squares Besides showing possible gene pairings, a Punnett square gives the probability of each pairing. It shows how often, on the average, a given pairing will occur. F f F FF F f f Ff f f 1:2:1 3:1 F1 Punnett Squares Punnett squares cannot tell which traits a specific offspring will exhibit but rather the chance, or odds, of having certain traits 1:2:1 3:1 Punnett Squares The number of boxes in a Punnett square does not stand for the number of offspring an organism will produce. The boxes represent the genetic possibilities of the offspring. Punnett Squares A small # of offspringmay not have the exact ratio of gene pairings. 1:2:1 3:1