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Cellular Transport Osmosis and Diffusion! IMPORTANT DEFINITIONS • DIFFUSION: movement of particles from higher concentration to lower concentration • OSMOSIS: diffusion of water across a selectively permeable membrane In a cell, water always tries to reach an equal concentration on both sides of the membrane! DIFFUSION OSMOSIS What controls OSMOSIS? 1) A concentration gradient (unequal distribution of particles on each side of a membrane). During osmosis, only water diffuses across the selectively permeable membrane. OSMOSIS ISOTONIC SOLUTIONS! • Concentration of dissolved substances in solution is the same as concentration of dissolved substances inside the cell. • Water inside cell is equal to water in solution. • Cells in isotonic solution do not experience osmosis and retain their normal shape. • EX: Immunizations are isotonic solutions so they do not damage the cells by gain or loss of water. HYPOTONIC SOLUTIONS! • Concentration of dissolved substances is lower in solution outside the cell than concentration inside the cell. • There is more water outside the cell than inside. • Cells in hypotonic solutions experience osmosis in which water moves through membrane into cell. • EX: In animal cells, the pressure inside cell increases causing the cells to swell and sometimes burst! • EX: In plant cells, the rigid cell wall prevents bursting, but the cells become more firm. CUCUMBERS IN THE MIST! HYPERTONIC SOLUTIONS! • Concentration of dissolved substances outside cell is higher than concentration inside cell. • There is more water inside cell than outside. • Cells in hypertonic solutions experience osmosis in which water moves through membrane to outside of cell. • Ex: In plant cells, membrane and cytoplasm shrink away from cell wall and plant wilts. • EX: In animal cells, the pressure decreases and the cells shrivel. Don’t be so SALTY! Passive Transport: Mosey on through… • Molecules pass through the membrane by diffusion requiring no extra energy. Ex: water & lipids • Ion Channels -type of passive transport that uses membrane proteins to pass certain ions. Exp. Cl- Na+ Ca2+ Passive Transport Passive Transport Facilitated Diffusion: Help them out a bit! Remember those transport proteins in the phospholipid bilayer? Here’s where they fit in! • Facilitated diffusion: passive transport across membrane with help of transport proteins. • Ex: sugars & amino acids Active Transport: ENERGY REQUIRED! • Active transport is the movement of materials through a membrane across a concentration gradient. • This requires energy to counteract the movement of diffusion from high to low concentrations! How does it work? 1) Transport protein called “carrier protein” binds with particle that is going to be transported. 2) Energy is required. 3) Proteins can continue this process over and over. -Active transport allows a particle movement into or out of a cell against a concentration gradient. Exp. Na out of the cell, K into the cell. How carrier proteins work! Active Transport Active Transport Cellular Transport What about the big boys? • ENDOCYTOSIS: cells surrounds and takes in material from environment by engulfing the material! YUMMY! • EXOCYTOSIS: cells expel materials from cell, such as waste or indigestible particles. GROSS! • Both endo and exocytosis are moving large masses of material and require energy (ACTIVE TRANSPORT!) Endocytosis Exocytosis