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Understanding the Microscope Honors Biology Compound Microscope • Contains a combination of two lenses Eyepiece or ocular lens • Lens closest to the eye Objective Lens • Lens closest to the specimen or object How to figure out magnification • Eyepiece • 10x • 10x • 10x X X X X objective = Magnification Nosepiece • Objectives fastened to this revolving structure Stage • Where you place your slide Stage clips • Hold slide in place Body Tube • Light travels through here Light Source • Provides light Diaphragm • Regulates the light reaching the objective lens Base • Supports the microscope Arm • Holds the body tube, acts as a handle Coarse focusing knob • Used to focus under low power only Fine focusing knob • Used to focus under high power only A microscope reverses and inverts the image of an object seen under it Before viewing under the microscope e While viewing under the microscope e Things moving under a microscope are actually moving in the opposite direction Things moving under a microscope are actually moving in the opposite direction Stereomicroscope • Binocular microscope • Object is seen in 3-D • Only used to see large objects • Does not reverse or invert images Transmission Electron Microscope • Uses a beam of electrons instead of light rays • 200,000x magnification • tissues have to be sliced really thin, dry and in a vacuum chamber • can’t be used with living material Scanning Electron Microscope • provides images with 3-D quality • can’t be used with living material Micromanipulator • Used to dissect cells, can remove nuclei • Knowledge of cells has been increased by manipulation and dissection of cells High – Speed Centrifuge • Spin at high speeds • Cell components sort out at different layers according to density Microscopic Measurements • Use the unit called the micrometer • 1/1000 of a meter How to convert millimeters to micrometers • Multiply by one thousand…or • Move the decimal point 3 places to the right