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POST-TREATMENT EVALUATION OF MAXILLARY CANINE POSITION IN ADOLESCENT CAUCASIAN MALES AND FEMALES John Katsis III, D.D.S. An Abstract Presented to the Graduate Faculty of Saint Louis University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Dentistry 2012 Abstract Introduction: Esthetic improvement is one of the primary reasons individuals seek orthodontic treatment. The maxillary canine is considered by many to have great importance for both function and esthetics. Limited information is available about the position of the maxillary canine in relation to skeletal landmarks and if the position can influence esthetic perception. Purpose: The purpose of this study was to evaluate the normal maxillary canine position in relation to skeletal landmarks, to determine post-treatment three-dimensional maxillary canine position with Cone Beam CT images, and to see if maxillary canine position could influence esthetic perception. Methods: The Bolton Standard template acted as the control sample and the maxillary canine position was determined by implementing a Cartesian coordinate system. The right and left maxillary canines of 48 males and 48 females who received orthodontic treatment were analyzed by digitization of Cone Beam CT volumes. The subject’s post- treatment smile photographs were ranked and quantified by nine orthodontic residents who completed a Q-sort. Correlations were determined between canine position and esthetic outcomes. Results: The only difference between 1 right and left canine position was the anterior-posterior position of the root apex. Statistically significant gender differences were found for the superior-inferior position of the right and left canine cusp tip, the mediallateral right and left canine root apex, and the mediallateral left canine cusp tip. No correlation was determined between the maxillary canine position and esthetic perception. Conclusion: The maxillary canine position in relation to skeletal landmarks was determined and does not appear to significantly impact esthetic perception according to this study. 2 POST-TREATMENT EVALUATION OF MAXILLARY CANINE POSITION IN ADOLESCENT CAUCASIAN MALES AND FEMALES John Katsis III, D.D.S. A Thesis Presented to the Graduate Faculty of Saint Louis University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Dentistry 2012 COMMITTEE IN CHARGE OF CANDIDACY: Professor Rolf G. Behrents, Chairperson and Advisor Professor Eustaquio A. Araujo Associate Clinical Professor Donald R. Oliver Associate Clinical Professor Ki Beom Kim i DEDICATION I dedicate this thesis to my parents. Throughout my life they have served as a source of inspiration on how to live a life of integrity, dedication, and commitment. I also would like to dedicate this to my siblings who have provided unwavering support in all my endeavors. ii ACKNOWLEDGEMENTS I would like to acknowledge the following individuals: Dr. Behrents for his mentorship and dedication to this project. I would not have been able to complete this project without him. Dr. Oliver for his support and help with the stylistic portion of writing my thesis. He was willing and eager to help at any time. Dr. Araujo for his creative input to the project. He is a wealth of knowledge and an excellent role model on how to lead a life of many passions. Dr. Ki Beom Kim for his help with the technological aspects of the project. His help was invaluable to the completion of the project. Dr. Heidi Israel for her help with the statistical analysis of the project. I would also like to thank my co-residents for their assistance and support while working on my thesis and throughout my entire residency. iii TABLE OF CONTENTS List of Tables...........................................vi List of Figures.........................................vii CHAPTER 1: INTRODUCTION...................................1 CHAPTER 2: REVIEW OF THE LITERATURE.......................3 Facial Esthetics in Orthodontics.....................3 Dental Esthetics in Orthodontics.....................4 Smile Esthetics in Orthodontics......................7 Tooth Morphology and Esthetic Perception.............9 Quantification of Esthetic Outcomes.................10 Quantification of Skeletal and Dental Relationships... .......................13 Advancements in Orthodontic Treatment...............14 Functional Importance of the Maxillary Canine.......16 Maxillary Canine Positional Characteristics in Relation to the Dental Arch....................18 Maxillary Canine Position in Relation to Soft-Tissue landmarks..........................20 Maxillary Canine Position in Relation to Skeletal Landmarks.............................21 Summary and Statement of Purpose....................25 References..........................................26 CHAPTER 3: JOURNAL ARTICLE Abstract............................................30 Introduction........................................32 Materials and Methods...............................36 Control Sample.................................36 Orientation of Control Sample..................36 Control Sample Cephalometric Landmarks.........38 Experimental Sample............................41 Orientation of Experimental Sample CBCT Volumes.......................41 Measurement Methods of Experimental Sample.....43 Establishing Data Points.......................43 Determination of Canine Angulation and Position..................................44 Q-sort.........................................45 Statistics.....................................47 Results.............................................50 Error Study....................................50 Control Sample Results.........................50 iv Descriptive Statistics of Experimental Sample.......................52 Scatter Plot Data..............................56 Maxillary Canine Position Patterns.............61 Esthetic Results...............................63 Esthetic Results in Relation to Canine Position...........................64 Discussion..........................................69 Three Dimensional Assessment of Maxillary Canine Position.................69 Maxillary Canine Position and Esthetic Perception.......................71 Conclusion..........................................74 Literature Cited....................................75 Appendix A (Histograms of linear distances from control sample)......................78 Appendix B (Graphs of best and worst esthetic Outcome XY and YZ canine position)........81 Appendix C (Photographs of subjects with highest and lowest combined esthetic scores)......82 Appendix D (Photographs of subjects with highest and lowest total canine position scores)..84 Vita Auctoris............................................86 v LIST OF TABLES Table 3.1: Cephalometric landmarks of control sample........................................39 Table 3.2: Bolton standard Cartesian coordinates.........51 Table 3.3: Bolton standard Cartesian coordinates.........51 Table 3.4: Bolton standard angular measurements..........51 Table 3.5: Descriptive statistics digitized landmarks of experimental sample........................52 Table 3.6: Descriptive statistics of angular measurements of experimental sample...........53 Table 3.7: Comparison of gender differences in experimental sample...........................54 Table 3.8: Comparison of right and left maxillary canine position in experimental sample...............55 Table 3.9: Correlations of esthetic scores and maxillary canine position scores........................67 vi LIST OF FIGURES Figure 2.1: Relationship of contact points, connectors, and embrasures...................6 Figure 2.2: Simon’s Facebow and Gnathostat devices......................................23 Figure 2.3: Simon’s Photostat device.....................24 Figure 3.1: Orientation of the PA tracing................37 Figure 3.2: Orientation of the lateral tracing......................................38 Figure 3.3: Cephalometric landmarks of PA tracing......................................40 Figure 3.4: Cephalometric landmarks of lateral tracing......................................40 Figure 3.5: Orientation of the CBCT volume...............42 Figure 3.6: Digitization of maxillary canine cusp tip and root apex................................44 Figure 3.7: Example of smile photograph..................46 Figure 3.8: Scatter plot of XY axis of right canine cusp tip.....................................56 Figure 3.9: Scatter plot of XY axis of right canine root apex....................................57 Figure 3.10: Scatter plot of ZY axis of right canine cusp tip....................................58 Figure 3.11: Scatter plot of ZY axis of right canine root apex...................................59 Figure 3.12: Scatter plot of XZ axis of right canine cusp tip....................................60 Figure 3.13: Scatter plot of XZ axis of right canine root apex...................................61 Figure 3.14: Histogram of esthetic Q-sort result.........63 vii Figure 3.15: Graph and correlation of esthetic scores vs. total canine position............65 Figure 3.16: Graph and correlation of esthetic scores vs. XY axis canine position..........65 Figure 3.17: Graph and correlation of esthetic scores vs. ZY axis canine position..........66 Figure 3.18: Graph and correlation of esthetic scores vs. XZ axis canine position..........66 Figure 3.19: Graph and correlation of total canine position scores vs. esthetic scores.........67 Figure 3.20: Histogram of linear distance from the norm of right canine cusp of the XY axis....78 Figure 3.21: Histogram of linear distance from the norm of right canine apex of the XY axis....78 Figure 3.22: Histogram of linear distance from the norm of right canine cusp of the ZY axis....79 Figure 3.23: Histogram of linear distance from the norm of right canine apex of the ZY axis....79 Figure 3.24: Histogram of linear distance from the norm of right canine cusp of the XZ axis....80 Figure 3.25: Histogram of linear distance from the norm of right canine apex of the XY axis....80 Figure 3.26: Graph of XY canine position of four best and four worst esthetic outcomes compared to Bolton Standard........81 Figure 3.27: Graph of ZY canine position of four best and four worst esthetic outcomes compared to Bolton Standard........81 Figure 3.28: Smile photographs of four subjects with highest combined esthetic scores.......82 Figure 3.29: Smile photographs of four subjects with lowest combined esthetic scores........83 viii Figure 3.30: Smile photographs of four subjects with highest combined canine position scores.....84 Figure 3.31: Smile photographs of four subjects with lowest combined canine position scores......85 ix CHAPTER 1: INTRODUCTION The improvement of facial and dental esthetics is one of the primary reasons individuals seek orthodontic treatment. Advancements in orthodontic treatment such as orthognathic surgery and temporary anchorage devices allow a clinician to effectively manipulate the dentition in three planes of space. The question arises as to where the teeth should be placed in order to maximize esthetics. Orthodontists have utilized a variety of diagnostic and treatment planning methods to achieve this. One such method developed by Simon, a German orthodontist in the early 20th century, utilized a facebow and articulator device to recreate the skeletal and dental relationship.1 He believed that individuals with normal relationships had the maxillary canine crown positioned along the orbital plane, defined as a frontal plane perpendicular to the midsagittal and the Frankfort horizontal planes.2 Other studies have examined the relationship of the canine to the orbital plane with conflicting results.2,3 Technological advancements, such as three-dimensional imaging, allow the orthodontist to accurately examine dental and skeletal relationships in three dimensions. 1 The purpose of this study is threefold. First, the normal maxillary canine position in relation to skeletal landmarks will be determined. Second, a post-treatment three-dimensional assessment of maxillary canine position utilizing CBCT volumes of Caucasian male and female subjects will be completed. Finally, the effect of the maxillary canine position on esthetic perception of frontal smiling photographs will be determined. 2 CHAPTER 2: REVIEW OF THE LITERATURE Facial Esthetics in Orthodontics Orthodontics is often referred to as an art and a science. The “art” portion of orthodontics is due to the fact that the clinician has the ability and responsibility to shape the dentition and surrounding tissues to create the most visually pleasing result possible. Although the orthodontic literature has a vast amount of information about esthetics that dates back over a century, a universal, objective way to define beauty on an individual basis may not be possible. The perception of facial esthetics has cultural, genetic, and sexual influences that vary significantly among individuals.4–6 Because of the subjective nature of esthetic judgment the evaluation of facial esthetics focuses primarily on symmetry and facial proportions.7 Even though esthetic preferences vary significantly among individuals, facial symmetry has been shown to routinely be positively correlated to esthetic perception. Facial symmetry is defined as the extent to which one side of the face mirrors the opposite side when divided vertically at the facial midline.5 3 Jones showed that individuals with high levels of facial symmetry were perceived to be more attractive and increased facial symmetry was also positively correlated with the perception of good health.6 In Little’s review of facial attractiveness, facial symmetry had positive correlations with growth rate, attractiveness, and fertility.5 The attainment of facial symmetry is an important consideration in orthodontic diagnosis and treatment planning. Dental Esthetics in Orthodontics In order to maximize smile esthetics, one must have a thorough understanding of dental esthetics. The shape and contour of the teeth has a great impact on dental esthetics. The maxillary central incisors should ideally have a width to height ratio of 0.8 with ranges reported in the literature between 0.66-0.8.8 Variations in this ratio can be due to reduced tooth mass, incomplete eruption, or aberrant gingival architecture. The position of the maxillary anterior teeth is important for smile esthetics. The dental midline relationship, symmetry, contact relationships, and tooth angulation all affect dental esthetics. 4 The dental midline is represented by the contact of the left and right maxillary central incisors. The dental midline should be coincident with soft tissue nasion and the base of the philtrum of the upper lip.9 The contact point of the mandibular central incisors should also be coincident with the maxillary dental midline. Dental symmetry is similar to facial symmetry in that it is determined by the extent the left and right maxillary anterior teeth mirror each other. The contact point among the maxillary teeth progressively moves more apically as the teeth move distally along the arch. The perceived contact area, also referred to as the connector, between the maxillary teeth diminishes moving from the midline to the distal portion of the arch. Dental embrasures are defined as the negative spaces incisal to the contact points of teeth. The incisal embrasure of the maxillary anterior teeth is the smallest at the dental midline between the right and left maxillary central incisor and should appear to grow larger moving distally through the arch.8 The contact and embrasure relationship of the teeth is influenced by the angulation of the tooth. According to Andrews, the maxillary anterior teeth should also exhibit a slight distal tip of the gingival portion of the crown when compared to the incisal portion.10 5 Figure 2.1: Relationship of contact points, connectors, and embrasures modified from Sarver.11 The gingival tissues framing the teeth play an important role in dental esthetics as well. The gingiva dictates the cervical shape of each tooth. In the absence of periodontal disease, the shape of the gingiva follows the cementoenamel junction and osseous crest. For an individual tooth the gingiva is shaped like an asymmetric arch with the apex of the arch slightly distal to the central axis of the tooth in the maxillary central incisors and maxillary canines. The apex of the gingiva in the maxillary laterals and mandibular incisors follows the long axis of the tooth.8,11 The gingival margins of the maxillary anterior teeth follow a “high-low-high” pattern in that the gingival margins of the maxillary central incisors and 6 canines have a more superior position when compared to the maxillary lateral incisors.8,9,11 Smile Esthetics in Orthodontics The position of the teeth within the mouth plays a significant role in the appearance of the smile. The term “smile arc” has been used to describe the relationship between the teeth and the lower lip in a posed smile. The smile arc is defined as “the relationship of the curvature of the incisal edges of the maxillary incisors and canines to the curvature of the lower lip in the posed smile.”12 The smile arc is related to the cant of the occlusal plane and can be significantly affected during treatment by excessive intrusion and inclination of the maxillary incisors.4,9,12 Some clinicians believe the smile arc must be maintained as a primary treatment goal. They utilize the smile arc to gauge bracket heights in an effort to minimize the flattening of the arc that often occurs with orthodontic treatment.9,12 Conversely, other research downplays the importance of the smile arc. Nanda and Burstone have demonstrated that the smile arc is influenced by head position and that it can be dramatically changed in the same individual simply by moving the head up and down.4 7 Another interaction between the teeth and the smile is the amount of space present lateral to the buccal surfaces of the maxillary posterior teeth. This space is referred to as the “buccal corridor” and the concept was originally developed as it related to denture fabrication by Frush and Fisher.13 Frush and Fisher warned that to eliminate the buccal corridor by the expansion of the dentition was characteristic of a denture and the presence of the buccal corridor created a more natural appearance. Buccal corridors have emerged in the contemporary orthodontic literature as a controversial topic. A study by Moore that digitally manipulated the transverse dental dimension in individuals found a positive correlation with reduced buccal corridors and improved smile esthetics.14 Hulsey found no correlation of buccal corridor size to smile esthetics when unaltered smile photographs of different subjects were judged by laypersons.15 A systematic review of smile esthetics found that in studies that digitally manipulated the buccal corridor in the same smile photograph, the reduction in the size of buccal corridors was positively correlated to improved smile esthetics. However studies that examined buccal corridor sizes in different subjects showed no correlation with excellent smile esthetics.16 8 The position of the lips in a smile is also of great importance to smile esthetics. The level of upper lip elevation while smiling, or the lip line, influences the maxillary anterior tooth and gingival display. In esthetic smiles, the upper lip is elevated to the height of the gingival margin of the maxillary anterior teeth.9,12 The lip line differs by gender with women having a higher lip line at rest. Because of this, women will have more incisor and gingival display when smiling. The lip line, both at rest and during smiling, decreases in height during the normal aging process.9 Sarver reports that an increased gingival display with smiling is youthful in appearance and may help to offset the normal appearance of aging.12 Tooth Morphology and Esthetic Perception Dental morphology of the anterior teeth is relatively unique to each individual. Differences exist in the roundness of the anterior teeth that can affect esthetic perception. Esthetic perception of anterior tooth morphology was evaluated among laypeople,17,18 restorative dentists,18 and orthodontists.18 In general, laypeople were less critical of differences in anterior tooth morphology and the shape of the maxillary canine did not significantly 9 influence a layperson’s esthetic perception.17 Restorative dentists tended to prefer males to have less rounded (more square) incisors and female incisor shape to be more rounded while orthodontists were less discriminating of incisor shape. Interestingly, orthodontist’s preferences for canine shape differed amongst the different type of incisor configuration with a preference to pointed canines in square incisors and rounded canines in rounded incisors. Overall, canine morphology was not as significant to dental esthetic perception as incisor morphology. The author’s attributed this to the fact that dental professionals primarily associate the canine with function and not esthetics. The reduced relative proportion of canine display in frontal smiling photographs could also be responsible for the reduced significance of canine morphology on esthetic perception.18 Quantification of Esthetic Outcomes Although esthetic determination is subjective and multifactorial,4 measurements exist that attempt to quantify esthetic outcomes. Two of the most common methods used to quantify perceived esthetic outcomes are the visual analog scale and the Q-sort. 10 The visual analog scale (VAS) is a method used to quantify how esthetically pleasing one finds an outcome. The individual utilizing the VAS places a hash mark along a linear scale that is typically 100 millimeters in length. In esthetic studies, one end of the linear scale would represent excellent esthetics and the other would represent poor esthetics. This hash mark can then be measured from either extreme to establish a numerical value of the rater’s perception. The VAS provides a result that is easy to interpret, although raters tend to avoid the extremes of the scale which can confound the results.19 The Q-sort is a method of evaluating esthetic outcomes developed by Stephensen that has the judge rank the treatment outcomes of a group of individuals.19 To accomplish this, the judge picks out the best and worst outcomes and separates them. The judge continues to select the best and worst remaining outcomes until all have been selected. Depending on the sample size, the rater will select progressively more subjects during each round of judgment. Once the Q-sort is complete, it will generate a quasi-normal curve that can be used to quantify objective outcomes.19 11 The Q-sort and VAS have been applied to orthodontic treatment outcomes by Schabel in two separate studies. In one study, the VAS and Q-sort were used to evaluate posttreatment smiles by orthodontists and laypersons to determine which was more reliable. Intraclass correlation coefficient measures of reliability found the Q-sort to be more reliable than the VAS. Correlation coefficients of the average VAS and Q-sort scores were very high (r=0.96 for both) but correlation coefficients of individual rating groups (orthodontist vs. laypersons, males vs. females) found a higher correlation with the Q-sort than the VAS.19 Another study by Schabel determined if any specific smile characteristic was consistently evident in both esthetic and unestethic treatment outcomes. Of the several parameters examined, the presence of mandibular incisors when smiling was the only smile characteristic examined that reliably predicted an unesthetic smile result.20 The lack of quantifiable and reliable esthetic factors further support the difficulty in quantifying esthetics in orthodontic treatment. 12 Quantification of Skeletal and Dental Relationships Orthodontists have utilized objective measures when diagnosing malocclusions to maximize treatment outcomes. The field of cephalometrics has extensively studied the composition and growth of the craniofacial complex. In the Bolton-Brush Growth Study, a longitudinal series of lateral and posterior-anterior (PA) cephalograms were taken annually of individuals to quantify facial growth. This study and many others like it has generated normative data about the position of the teeth, skeleton, and supporting soft tissues. Clinicians utilize this data in conjunction with the clinical exam to assist in diagnosis, treatment planning, and to assess growth and treatment outcomes. The advent of cone beam computed tomography (CBCT) scans in orthodontics now allows the clinician unrestricted views of the dentition and skeleton. Cone Beam CT scans are so named for the cone shaped x-ray beam that utilizes a fraction of the radiation when compared to traditional multislice computed tomography scans.21 A lateral and frontal cephalometric film can be constructed from the CBCT volume. Studies have examined the accuracy of measurements taken directly from the CBCT volume22 as well as measurements comparing constructed lateral21,23 and PA 13 cephalometric radiographs.24 In general, CBCT provide a very accurate representation of dental and skeletal anatomy.22 Lateral cephalometric radiographs compared to CBCT constructed lateral cephalometric radiographs of dry skulls showed statistically significant differences in several standard measures, however these differences were clinically insignificant when considering standard error of landmark identification.21 A similar study of the differences between constructed and standard PA cephalometric radiographs found minor, clinically insignificant differences in distance measurements, however significant differences were found comparing many standard angular measurements.24 The reason for these differences were attributed to errors in head positioning of the CBCT due to a lack of ear rods that are routinely used in standard cephalometric radiology. Advancements in Orthodontic Treatment The field of orthodontics has undergone significant advances in treatment efficacy. One of the most significant advances in dentistry and orthodontics are dental implants. Originally for restorative purposes, the use of dental implants has expanded into the field of 14 orthodontics. The first published case utilizing implants for orthodontic purposes was in 1983 by Creekmore and Ecklund for maxillary incisor intrusion.25,26 Years of research and development in this field has yielded an abundance of literature and treatment modalities. With the use of mini-screw implants, an orthodontist has more control than ever of the dentition. Orthognathic surgery is another such advance that allows for significant movements of the maxilla, mandible, and the dentition well beyond the limits of conventional orthodontics with or without growth modification.7 Dramatic skeletal, dental, and soft-tissue changes are possible with surgical and orthodontic interdisciplinary care. Now an orthodontist can visualize and diagnose a malocclusion in three dimensions and can effectively manipulate the dentition and supporting structures in three planes of space. The question arises of where exactly to place the teeth to maximize treatment outcomes. Orthodontists have relied upon the position of the maxillary canine in orthodontic diagnosis and treatment planning. 15 Functional Importance of Maxillary Canine The maxillary canine is a considered by many to be of utmost importance for the development of a functional occlusion.27 The concept of “canine protected occlusion” is based on a complete separation of the balancing side cusps and contacts during a lateral excursive movement.27,28 The advocates of canine protected occlusion cite anthropological,29 electromyographic,27 and pathological30 evidence as the basis of their clinical decision making. In theory, the long root and strong periodontal support of the canine is best suited to bear the brunt of occlusal forces during function.29 Some electromyography studies have shown a decrease in masticatory muscle activity in canine protected occlusions.27,31 Ronald Roth, one of the pioneers of canine protected occlusion, advocated orthodontic and equilibration therapy to eliminate balancing interferences, which he believed to be in “close association between the severity of temporomandibular joint pain-dysfunction and the location of balancing interferences on tooth-guided excursions.”30 Although the principles of canine protected occlusion (CPO) appear valid and therapeutic anecdotally, current literature does not completely support its benefits. 16 The maxillary canine’s morphology and high level of periodontal support is not in question, however the reduction in muscle activity with canine protected occlusion and reduction in temporomandibular joint dysfunction (TMD) has not held up to current scientific scrutiny. Electromyography studies have been shown to have low levels of reliability and a significant reduction in muscular activity with CPO has not been found consistently. also unclear. The role of occlusion in TMD is Bruxism and parafunction have been shown to be independent of occlusal relationships and no causal relationship has been found to support the notion that CPO, or any occlusal scheme, can prevent or cure TMD.28,32 Parafunction, bruxism, and TMD are multi-factorial in nature and the current therapeutic recommendations endorsed by the American Dental Association supports management of symptoms and therapy aimed at the reduction in damage to the occlusal system and supporting structures. Orthodontic and restorative therapies are not advocated by the American Dental Association as a cure or a method to prevent TMD.32 17 Maxillary Canine Positional Characteristics in Relation to the Dental Arch The arrangement of all the teeth in an esthetically pleasing configuration is one of the primary objectives of orthodontic therapy. The quantification of the three- dimensional position of the dentition was the focus of Andrews’ study on tooth positions in untreated, excellent occlusions. He examined the dental configuration of 120 diagnostic models and quantified the intra-arch relationships of the crowns of individual teeth. In this study, the maxillary canines exhibited a natural distal inclination of the root compared to the crown. He developed a novel system to gauge the buccal-lingual inclination of the dental crown by drawing a line tangent to the center of the buccal surface of the dental crown and compared it to a line drawn perpendicular to the occlusal plane. He found the maxillary canine to exhibit a lingual crown inclination, which he denoted as negative crown torque.10 He furthered his research and developed brackets with a built-in tip value of +6˚ for non-extraction cases and negative torque values built into the bracket base.33 Although Andrews was not the first to come up with the notion of a pre-adjusted appliance,33 his research and 18 bracket system were instrumental in the technological advancement of the specialty. Three-dimensional imaging advancements have allowed retesting of Andrews’ research with consideration of the root and crown configuration in relation to the occlusal plane. A novel system developed by Tong et al examined the dental angulations on CBCT volumes of 76 untreated patients with near-normal occlusions. The system implemented oriented two points (center of the crown and the center of the root) of each individual tooth and measured the long axis against the occlusal plane. This method was similar to Andrews’ but takes into account the root in addition to the crown. The mesiodistal angulation of the right and left maxillary canine was 11.99 and 10.79 degrees, a statistically significant difference. The faciolingual inclination of the right and left maxillary canine was 20.33 and 21.18 degrees respectively and was not statistically significant. Although this study provided valuable insight into the three-dimensional arrangement of the dentition within the arch, more research is needed to develop and confirm normative data for the teeth. 19 Maxillary Canine Position in Relation to Soft Tissue Landmarks Common protocol in prosthodontic education is to place the maxillary canines “at the corner of the mouth.”34 Studies examining this have found variability among the canines in relation to the corner of the mouth and maxillary canines tend to be positioned mesial to the corner of the mouth. A study that directly measured the canine position in adolescent males found a statistically significant tendency for the right canine to be located 3.14 millimeters (mm) from cusp tip to the corner of the mouth and was 3.00 mm for the left canine.34 Another study evaluating vertical canine position in relation to the lips found that the vertical exposure of the maxillary canine measured against the resting lip was more consistent than maxillary incisor exposure among all age groups evaluated and also by gender. The average canine exposure in females was 0 mm (coincident with upper lip) with a range of -2 mm (inferior to the upper lip) to +2 mm (superior to the upper lip). The average canine exposure in males was -0.5 mm with a range of -3 mm to +2mm.35 Although resting lip position in relation to anterior tooth display is a helpful diagnostic aid, it is not used routinely by orthodontists. 20 Neither of these studies utilized skeletal landmarks when establishing canine position and cannot be applied to radiographic diagnostic measures used routinely in orthodontics. Maxillary Canine Position in Relation to Skeletal Landmarks A review of the literature examining the normal position of the maxillary canine in relation to the skull yielded few results. In 1924 Simon presented a paper about his novel method of orthodontic diagnosis which was based on skeletal and dental position in relation to the orbital plane. The orbital plane is defined as “a frontal plane determined by the lowest point of the infraorbital ridge and at right angles to the median sagittal and the Frankfort horizontal planes.”2 Simon developed his orbital plane theory from dried skulls by taking a sample of “several hundred cases of jaws with correct anatomical occlusion and examined them with the gnathostat.”1 He found that “in most of these cases the orbital plane passes through the cusps of the maxillary canines.” According to Simon, in normal skulls the orbital plane would also be coincident with the mandible at gnathion. He developed a system of diagnosis and treatment planning that would 21 classify both the skeletal and dental relationship. This would also serve as the basis of his treatment decisions.1 To determine the orbital plane on an individual basis Simon utilized a facebow device to register the occlusal relationship and then transferred this to an articulator. His facebow device recorded the patient’s occlusion in relation to Frankfort horizontal and also recorded the position of left and right orbitale. After the study models were mounted on his articulator Simon had a record of the patient’s occlusion in relation to Frankfort horizontal and the orbital plane. He had previously ascertained that the mid-sagittal plane was best represented by two points along the mid-palatal raphe by studying dried skulls. This set-up was referred to as a “gnathostat model.” 22 Figure 2.2: Simon’s facebow and gnathostat devices utilized to record the patient’s occlusion modified from his original journal article.1 Simon also developed a system of taking patient photographs that positioned the patient with Frankfort horizontal parallel to the floor and the orbital plane perpendicular to Frankfort horizontal.1 This system was developed prior to the advent of cephalometrics and therefore skeletal measurements could not be directly assessed on individual patients. 23 Figure 2.3: Photostat apparatus developed by Simon used for diagnosis and treatment planning modified from his original journal article.1 A study evaluating the maxillary canine position in dry skulls and the relation to the orbital plane was presented in 1926 by Oppenheim. He measured 159 skulls with “normal” occlusions and reported that porion-orbitalcanine angle was on average 104.5 degrees with a range of 96 to 115 degrees, an angulation greater than what Simon originally theorized. In skulls with Class II, division 1 malocclusions the range was 98 to 120 degrees with an average porion-orbital-canine angle of 109 degrees.2 The author was unable to find a study re-examining Simon’s orbital plane theory in vivo utilizing cephalometrics. 24 Summary and Statement of Purpose An improvement in facial and dental esthetics is a primary motivator in seeking orthodontic care. The orthodontic and dental literature has an abundance of information about improving esthetics and treatment outcomes. The author was unable to find any current literature examining maxillary canine position in relation to the skull. Additionally, little information is available about the influence of maxillary canine position on dental esthetics, even though it is considered to be one of the most critical teeth in the mouth for both esthetics and function. The purpose of this study is threefold. First, the normal maxillary canine position in relation to skeletal landmarks will be determined. Second, a post- treatment three-dimensional assessment of maxillary canine position utilizing CBCT volumes of Caucasian male and female subjects will be completed. Finally, the effect of the maxillary canine position on esthetic perception of frontal smiling photographs will be determined. 25 References 1. Simon PW. On gnathostatic diagnosis in orthodontics. Int J Orthod Oral Surg Radiogr. 1924(X):755–785. 2. Bercea MN. Review of an Article by Professor Dr. A. Oppenheim on “Prognathism from the Anthropolgical and Orthodontic Viewpoints”. Angle Orthod. 1928;100-108. 3. Connolly CJ. Relation of the orbital plane to position of teeth. Am J Phys Anthrop. 1927;(10):71–78 4. Burstone CJ, Nanda R. JCO Interviews, Part 1 facial esthetics. J Clin Orthod. 2007;41(2):79-87. 5. Little AC, Jones BC, DeBruine LM. Facial attractiveness: evolutionary based research. Philos T Roy Soc B. 2011;(366):1638–1659. 6. Jones BC, Little AC, Penton-Voak IS, Tiddeman BP, Burt DM, Perrett DI. Facial symmetry and judgements of apparent health Support(sic) for a “good genes” explanation of the attractiveness-symmetry relationship. Evol Hum Behav. 2001;(22):417-429. 7. Proffit WR, Fields HW, Sarver DM. Contemporary Orthodontics. 2007;St. Louis: Mosby Elsevier. 8. Sarver DM. Principles of cosmetic dentistry in orthodontics: Part 1. Shape and proportionality of anterior teeth. Am J Orthod Dentofacial Orthop. 2004;126(6):749–753. 9. Sabri R. The eight components of a balanced smile. J Clin Orthod. 2005; 39(3): 155-167. 10. Andrews LF. The six keys to normal occlusion. Am J Orthod. 1972;62(3):296-309. 11. Sarver DM, Yanosky M. Principles of cosmetic dentistry in orthodontics: part 2. Soft tissue laser technology and cosmetic gingival contouring. Am J Orthod Dentofacial Orthop. 2005;127(1):85–90. 26 12. Sarver DM. The importance of incisor positioning in the esthetic smile: The smile arc. Am J Orthod Dentofacial Orthop. 2001; 120(2):98-111. 13. Frush JP, Fisher RD. The dynesthetic interpretation of the dentogenic concept. J Prothet Dent.1958; 8(4):558581. 14. Moore T, Southard KA, Casko JS, Qian F, Southard TE. Buccal corridors and smile esthetics. Am J Orthod Dentofacial Orthop. 2005; 127(2):208-213. 15. Hulsey CM. An esthetic evaluation of lip-teeth relationships present in the smile. Am J Orthod.1970; 57(2):132-144. 16. Janson G, Branco NC, Fernandes TMF, Sathler R, Garib D, Lauris JRP. Influence of orthodontic treatment, midline position, buccal corridor and smile arc on smile attractiveness. A systematic review. Angle Orthod. 2011; 81(1):153-161. 17. Heravi F, Rashed R, Abachizadeh H. Esthetic preferences for the shape of anterior teeth in a posed smile. Am J Orthod Dentofacial Orthop 2011;139(6):806–814. 18. Anderson KM, Behrents RG, McKinney T, Buschang PH. Tooth shape preferences in an esthetic smile. Am J Orthod Dentofacial Orthop. 2005;(128):458–465. 19. Schabel BJ, McNamara JA , Franch L, Baccetti T. Q-sort assessment vs visual analog scale in the evaluation of smile esthetics. Am J Orthod Dentofacial Orthop. 2009;135(4):S61-71. 20. Schabel BJ, Franch L, Baccetti T, McNamara JA. Subjective vs objective evaluations of smile esthetics. Am J Orthod Dentofacial Orthop. 2009;135(4):S72-79. 27 21. van Vlijmen OJC, Bergé SJ, Swennen GRJ, Bronkhorst EM, Katsaros C, Kuijpers-Jagtam AM. Comparison of cephalometric radiographs obtained from cone-beam computed tomography scans and conventional radiographs. J Oral Maxillofac Surg. 2009;67(1):92–97. 22. Baumgaertel S, Palomo MJ, Palomo L, Hans MG. Reliability and accuracy of cone-beam computed tomography dental measurements. Am J Orthod Dentofacial Orthop. 2009;136(1):19-25. 23. Moshiri M, Scarfe WC, Hilgers ML, Scheetz JP, Silveira AM, Farman AG. Accuracy of linear measurements from imaging plate and lateral cephalometric images derived from cone-beam computed tomography. Am J Orthod Dentofacial Orthop. 2007;132(4):550-560. 24. van Vlijmen OJC, Maal TJJ, Berge´ SJ, Bronkhorst EM, Katsaros C, Kuijpers-Jagtman AM. A comparison between two-dimensional and three-dimensional cephalometry on frontal radiographs and on cone beam computed tomography scans of human skulls. Eur J Oral Sci 2009;117:300–305. 25. Creekmore TD, Eklund MK. The possibility of skeletal anchorage. J Clin Orthod 1983;4(4):266–269. 26. Papadopoulos MA, Tarawneh F. The use of miniscrew implants for temporary skeletal anchorage in orthodontics: A comprehensive review. Oral Surg Oral Med O 2007;103(5):e6–e15. 27. Okeson JP. Management of Temporomandibular Disorders and Occlusion. 2008;St. Louis: Mosby Elsevier. 28. Rinchuse DJ, Kandasamy S, Sciote J. A contemporary and evidence-based view of canine protected occlusion. Am J Orthod Dentofacial Orthop 2007;132(1):90–102. 29. D’Amico, A. The canine teeth: normal functional relation of the natural teeth of man. J S Calif Dent Assoc 1958;26:6-23. 28 30. Roth RH. Temporomandibular pain-dysfunction and occlusal relationships. Angle Orthod 1973;43(2):136–152. 31. Williamson EH, Lundquist DO. Anterior guidance: its effect on electromyographic activity of the temporal and masseter muscles. J Prosthet Dent 1983;49(6):816–823. 32. Management of temporomandibular disorders. National Institutes of Health Technology Assessment Conference Statement. JADA 1996;127(11):1595–1606. 33. Andrews LF. The straight-wire appliance. Br J Orthod 1979;6(3):125–143. 34. Parkash H, Bhalla LR, Khanna VK. Position of maxillary canine tip in relation to corner of mouth. J Indian Dent Assoc 1970;42(4):98–104. 35. Misch CE. Guidelines for maxillary incisal edge position-a pilot study: the key is the canine. J Prosthodont 2008;17(2):130–134. 29 CHAPTER 3: JOURNAL ARTICLE Abstract Introduction: Esthetic improvement is one of the primary reasons individuals seek orthodontic treatment. The maxillary canine is considered by many to have great importance for both function and esthetics. Limited information is available about the position of the maxillary canine in relation to skeletal landmarks and if the position can influence esthetic perception. Purpose: The purpose of this study was to evaluate the normal maxillary canine position in relation to skeletal landmarks, to determine post-treatment three-dimensional maxillary canine position with Cone Beam CT images, and to see if maxillary canine position could influence esthetic perception. Methods: The Bolton Standard template acted as the control sample and the maxillary canine position was determined by implementing a Cartesian coordinate system. The right and left maxillary canines of 48 males and 48 females who received orthodontic treatment were analyzed by digitization of Cone Beam CT volumes. The subject’s post- treatment smile photographs were ranked and quantified by nine orthodontic residents who completed a Q-sort. Correlations were determined between canine position and 30 esthetic outcomes. Results: The only difference between right and left canine position was the anterior-posterior position of the root apex. Statistically significant gender differences were found for the superior-inferior position of the right and left canine cusp tip, the mediallateral right and left canine root apex, and the mediallateral left canine cusp tip. No correlation was determined between the maxillary canine position and esthetic perception. Conclusion: The maxillary canine position in relation to skeletal landmarks was determined and does not appear to significantly impact esthetic perception according to this study. 31 Introduction The improvement of facial and dental esthetics is one of the primary reasons individuals seek orthodontic treatment. A large body of orthodontic literature exists on the subject of dental esthetics. Esthetic factors such as a high-degree of facial symmetry, an upper lip-line upon smiling that fully displays the upper incisors, and wellproportioned dental and gingival architecture have been consistently found to improve esthetic perception.1–6 Conversely, esthetic factors such as smile-arc consonance, the extent of buccal corridors, and the presence of the golden proportion in soft and hard tissue is highly contentious within the orthodontic literature.7–10 This stems from the difficulty in objectifying and quantifying esthetics, a topic that is greatly affected by cultural and personal influences.1,11 Methods exist to attempt to quantify esthetic outcomes. One such method is the Visual Analog Scale (VAS), which has a judge place a hash mark on a linear scale that is typically 100 millimeters in length. In an esthetic study, one end would represent an excellent esthetic outcome and the other would represent a poor 32 esthetic outcome. The hash mark on the visual analog scale can be measured to quantify a rater’s perception. Although the VAS is a method that is easy to complete and easy to interpret, judges tend to avoid extremes which confounds the results.12 Another method to quantify esthetic outcomes is with a Q-sort. A Q-sort involves a judge to separate the best and worst esthetic outcomes. The judge continues to separate more of the best and worst remaining outcomes until a quasi-normal curve is generated. These two methods of quantifying esthetics were examined in orthodontic treatment outcomes by Schabel et al, they found that the Qsort was more reliable and had a higher correlation among raters than the VAS.12 Orthodontists rely on a variety of diagnostic methods to plan treatment in order to maximize esthetics, stability, and function. Establishing a problem list and treatment plan for each individual patient involves a thorough assessment of the soft tissue characteristics, skeletal relationships, and dental configuration. Traditionally, orthodontic diagnosis was based on a lateral cephalogram, which is a two-dimensional representation of a three-dimensional object. With the advent of Cone-Beam 33 Computed Tomography (CBCT) a new era of three-dimensional diagnosis and treatment planning is on the horizon. Orthodontic diagnosis has focused primarily upon the position of the incisors and the molars in relation to the other teeth, the skull, and the supporting soft tissue. Interestingly, little information is available about maxillary canine position in both normal and abnormal dental and skeletal relationships, even though the canine is considered by many to be of great importance to occlusion and function.13,14 In the early 20th century German orthodontist Simon, developed a method of diagnosis and treatment planning hinged upon the maxillary canine’s position to the orbital plane.15 The orbital plane is defined as “a frontal plane determined by the lowest point of the infraorbital ridge and at right angles to the median sagittal and the Frankfort Horizontal planes.”16 Simon’s method of diagnosis was dependent upon a sophisticated facebow and articulator system that was able to reproduce a patient’s occlusion in relation to Frankfort Horizontal, the mid-saggital plane, and the orbital plane. Simon used his articulator system and measured dry skulls of patients with normal occlusion. He reported that the orbital plane passed through the 34 maxillary canine and the embrasure between the mandibular canine and first premolar in the majority of these cases. Simon’s theory was re-evaluated by Oppenheim in 1928 with conflicting results. Oppenheim found that the maxillary canine angle was not perpendicular to Frankfort Horizontal as reported by Simon, but was more procumbent with an average of 104.5 degrees.16 Both of these studies were completed prior to the advent of cephalometrics. A review of the literature did not yield a current study examining the relationship between maxillary canine position and skeletal landmarks. The purpose of this study is threefold. First, the normal maxillary canine position in relation to skeletal landmarks will be determined. Second, a post-treatment three-dimensional assessment of maxillary canine position utilizing CBCT volumes of Caucasian male and female subjects will be completed. Finally, the effect of the maxillary canine position on esthetic perception of frontal smiling photographs will be determined. 35 Materials and Methods Control Sample The control sample was composed of the Bolton Standard Cephalometric Template of 15 year old males and females. The Bolton Standard Template was created as a composite average of 32 males and 32 females selected from the Bolton-Brush Longitudinal Growth Study. The subjects in the Bolton Standard group had no orthodontic treatment and were previously deemed to have excellent facial esthetics, dental esthetics, and occlusal relationships. The Bolton Standard Template is comprised of both a lateral and posterior-anterior (PA) tracing of excellent quality. Orientation of Control Sample A Cartesian coordinate system was utilized to uniformly orient the PA and lateral tracings. The x-axis of the PA tracing was established through left and right orbitale. The y-axis of the PA cephalogram was constructed through the facial midline at a perpendicular to x-axis. The intersection of the x and y axis marked the (0,0) point. Because the Bolton Standard Template is constructed from a series of PA cephalograms, which has the patient face the x-ray film, the right and left side of the tracing 36 are reversed. The Cartesian coordinate system for the lateral cephalogram was established with Frankfort Horizontal as the z-axis (horizontal). The orbital plane, as defined by Simon, was constructed by a perpendicular plane from Frankfort Horizontal through orbitale and represented the y-axis (vertical). The (0,0) mark on the lateral cephalogram was at the intersection of Frankfort Horizontal and the orbital plane. Figure 3.1: Orientation of the PA tracing. 37 Figure 3.2: Orientation of the lateral tracing. Control Sample Cephalometric Landmarks The landmarks identified on the lateral cephalogram were canine cusp tip, canine root apex, orbitale, and porion. The landmarks identified on the PA cephalogram were right maxillary canine cusp tip, right maxillary canine root apex, left maxillary canine cusp tip, left maxillary canine root apex, right orbitale, left orbitale, right ear rod, and left ear rod. 38 Table 3.1: Cephalometric Landmarks of Control Sample Porion (PO) Orbitale (OR) Canine Cusp Tip (CCT) Canine Root Apex (CRA) The midpoint of the line connecting the most superior point of the radiopacity generated by each of the two ear rods of the cephalostat.17 The lowest point on the average of the right and left borders of the bony orbit.17 The cusp tip of the maxillary canine. The apex of the maxillary canine. Maxillary right and left canine cusp tip and apex were identified and the (x,y) coordinates recorded on the PA cephalogram. The (z,y) coordinates were recorded on the lateral cephalogram of the canine cusp tip and root apex. The (z,y) coordinates from the lateral tracing were applied to the right and left canine even though only one canine is drawn on the Bolton Standard Template. 39 Figure 3.3: Cephalometric landmarks of PA tracing. Figure 3.4: Cephalometric landmarks of lateral tracing. 40 Experimental Sample The experimental sample was composed of the posttreatment Cone Beam CT (CBCT) scans of 48 Caucasian males and 48 Caucasian females who had received orthodontic treatment at Case Western Reserve University. The subjects were selected based on the following criteria: a) The subject was Caucasian. b) A post-treatment CBCT volume was available. c) A post-treatment photograph of high quality was available that showed the frontal smile. d) The subject was 15 years old. e) The subject had both right and left maxillary canines. f) The subject did not have maxillary canine substitution treatment. g) The subject did not have significant restorative needs after orthodontic treatment. For example, subjects that needed restorations for missing and/or traumatized teeth or prosthetic enhancement for irregularly shaped teeth were excluded. Orientation of Experimental Sample CBCT Volumes The Cartesian coordinate system is determined by the orientation of the volume and therefore a reproducible 41 system must be implemented. Previous research using this method oriented the skull into an x, y, and z axis’s.18 The axial plane was represented by Frankfort Horizontal and was composed of a plane through right and left orbitale extending through right and left porion. The mid-saggital plane was created at a right angle to x-axis through the anatomic facial midline determined by inspection through crista galli and confirmed through sella turcica. The frontal plane was constructed to mimic the orbital plane and was generated by creating a plane perpendicular to Frankfort Horizontal through right and left orbitale. Figure 3.5: Orientation of the CBCT volume. A) X-Z axis along Frankfort Horizontal. B) Y-Z axis perpendicular to Xaxis through mid-sella. C) X-Y axis connecting right and left orbitale from the frontal view. 42 Measurement Methods of Experimental Sample The CBCT volumes of the experimental sample were evaluated using Dolphin 3D Imaging Software. The multi- planar viewing window allowed for simultaneous visualization of the frontal, sagittal, and coronal planes. The CBCT volume was rotated and orientated to allow visualization of the pulp chamber of the right and left maxillary canine. Orientation of the long axis of the maxillary canine was confirmed in all three planes of space. Establishing Data Points The maxillary canine position was quantified by digitizing the cusp tip and root apex of the right and left canines. The order the points digitized was always as follows: 1) Right maxillary canine cusp tip 2) Right maxillary canine root apex 3) Left maxillary canine cusp tip 4) Right maxillary canine root apex The digitized point’s accuracy was confirmed by visual inspection of the other planes. The coordinates were then exported to Microsoft excel according to the Cartesian 43 coordinate system with (0,0,0) representing the intersection of the X, Y, and Z axes. Figure 3.6: Digitization of maxillary canine cusp tip and root apex in Dolphin Imaging Software. Determination of Canine Angulation and Position Basic trigonometry was used to determine the lateral and frontal angulation of the maxillary canines. With the maxillary canine representing the hypotenuse of a right triangle, the two remaining sides were determined by finding the differences between the horizontal and vertical points. For example, when examining the frontal canine angle: 44 X2-X1 = Horizontal Leg of Triangle (A) Y2-Y1 = Vertical Leg of Triangle (B) The Pythagorean Theorem was then used to determine the length of the maxillary canine (hypotenuse) by the formula: A2 (Horizontal Leg) + B2 (Vertical Leg) = C2 (Hypotenuse) The angle of the maxillary canine was determined by finding the inverse tangent. The formula for determining this angle is: Inverse tangent(Opposite/Adjacent) = Canine Angle or Inverse tangent (A/B) = Canine Angle The maxillary canine angulation from the lateral view was determined in the exact same way with the z-axis orientated along the horizon and maintaining the y-axis as the vertical axis. The maxillary canine angulation was determined by using inverse tangent as described previously. Q-Sort As described earlier, the Q-sort method of judging esthetics is considered to be an effective and reliable way to quantify esthetics. Traditionally a Q-sort requires the judge to rate a sample of 96 subjects. Previous studies12,19 have shown that 45 a sample size of 48 is adequate to satisfy the requirements of a Q-sort. In this study, two separate Q-sorts were completed of photographs of 48 male subjects and 48 female subjects to assess if any gender differences exist. Post-treatment photographs of the frontal smile were cropped to 3 x 5 inches. Confounding anatomic factors such as the nose, eyes, and hair were cropped from the photograph. Photoshop® Software was used to remove any blemishes that could influence rater’s perception. Figure 3.7: Example of smile photograph used during Q-sort. Nine third year orthodontic residents from the Graduate Orthodontics Department of Saint Louis University Center for Advanced Dental Education volunteered to perform the Q-sort. The subjects were given consent forms in 46 accordance to Saint Louis University’s Institutional Review Board and agreed to participate in the study. Each resident completed two separate Q-sorts, one of the male subjects and one of the female subjects. The instructions given to each judge were as follows: “From the 48 photographs please pick the two best and two least esthetic smiles based only on the available photographs. Set aside the two best and the two worst at separate ends of the table. From the remaining 44 photographs please select the four best and four least esthetic smiles and set aside. From the remaining 36 photographs please select the five best and five least esthetic smiles. From the remaining 26 photographs please select the eight best and eight least esthetic smiles remaining. The ten photographs remaining should represent what you consider to be average looking smiles.” Statistics Statistics were calculated with SPSS 20.0 Statistical Software (SPSS, Inc., Chicago, IL). Descriptive statistics of the experimental sample were determined of the coordinate and angular measures. The x, y, and z coordinates of the canine cusp tip and apex of each experimental subject was compared to the control sample by 47 measuring the linear distance from the control value. To accomplish this six total scatter plots were generated of the canine cusp tip and root apex of the maxillary right canine. The scatter plot was orientated along the XY, YZ, and XZ axes and consisted of the control sample and all 96 subjects from the experimental sample. To determine the linear distance between the normal canine coordinates and experimental subject coordinates right angle trigonometry was utilized. The horizontal and vertical distance from the norm of each subject was determined. This created a right triangle with the hypotenuse representing the linear distance between the two points. The Pythagorean Theorem is used to calculate the length of the hypotenuse. A2 (Horizontal Leg) + B2 (Vertical Leg) = C2 (Hypotenuse) The Q-sort was analyzed by assigning a point value to each subject depending upon the esthetic category he/she was placed. This generated a system to quantify, rank, and distribute the esthetic outcomes. Q-sort distributions were also generated of the linear distances of the canine cusp tip and root apex of the maxillary right canine. Correlations were calculated between the best and worst 48 esthetic results as well as the best and worst canine position results. To calculate reliability of the study 12 of the CBCT volumes were re-orientated and re-digitized. The Cronbach’s Alpha measure of reliability was utilized. A Cronbach’s Alpha greater than 0.80 is considered reliable. 49 Results Error Study The Cronbach’s Alpha was 0.876 for the total combined digitized landmarks and re-orientation. This value indicates that the repeated measurements were not statistically significant from the original data points. Control Sample Results The x, y, and z coordinates of the maxillary canines from the control sample were determined from the Bolton Standard Templates. Because only one canine was traced for the lateral cephalogram template, the y and z data was applied to both the right and left side. The y coordinate could be determined from both the lateral and frontal template but the y value from the frontal template was used exclusively. The y values of the canine cusp differed by 4% for the canine cusp and apex. 50 Table 3.2: Bolton Standard Cartesian Coordinates – Male/Female PA Tracing (mm) Right Canine Cusp Right Canine Apex Left Canine Cusp Left Canine Apex X -17 Y -50 -13 -23 19 -50 14 -23 Table 3.3: Bolton Standard Cartesian Coordinates – Male/Female Lateral Tracing (mm) Right Canine Cusp Right Canine Apex Left Canine Cusp Left Canine Apex Z 7.5 Y -52 3.5 -24 7.5 -52 3.5 -24 The angular measures of the control sample were determined by direct measurement. The frontal canine angle is the angle of the maxillary canine to the mid-sagittal plane. The lateral canine angle is the angle of the maxillary canine to Frankfort Horizontal. Table 3.4: Bolton Standard Angular Measurements – Male/Female (degrees) Right Canine Frontal Angle Left Canine Frontal Angle Lateral Canine Angle 9 10 99 51 Descriptive Statistics of Experimental Sample Descriptive statistics of the x, y, and z coordinates of the experimental sample were calculated from the CBCT volumes. The x coordinates, which represent the right and left distance from the midline, tended to have the smallest range and standard deviation compared to y and z coordinates. Table 3.5: Descriptive Statistics of Digitized Landmarks (mm) of Experimental Sample – Total Sample (n=96) Right Canine Cusp X Right Canine Cusp Y Right Canine Cusp Z Right Canine Apex X Right Canine Apex Y Right Canine Apex Z Left Canine Cusp X Left Canine Cusp Y Left Canine Cusp Z Left Canine Apex X Left Canine Apex Y Left Canine Apex Z Mean (SD) -17.5 (1.4) Median Mode Range (Min/Max) -17.3 -16.7 8.5 (-22/-13.5) -47.7 (3.2) -47.1 -45.4 14.4 (-55.8/-42.6) 10.6 (3.3) 11.2 9.2 16.1 (1.8/-17.9) -14.1 (1.8) -14.2 -14.2 9.3 (-19.1 - -9.8) -24.5 (2.7) -24.2 -23.8 12.7 (-32.1/-19.4) 4.7 (2.5) 4.9 7 12 (-1/11) 17.2 (1.4) -47.6 (3.1) 10.7 (3.2) 13.9 (1.8) -24.4 (2.8) 4.4 (2.4) 17.1 16.6 -47.2 -46.1 11.0 11.5 13.9 14.4 -24.3 -21.2 4.6 5.8 6.2 (14.4/20.6) 15 (-55.6/-40.6) 15.1 (3.5/18.6) 10 (9.3/19.3) 12.8 (-32.3/-19.5) 10.6 (-0.7/9.9) 52 Table 3.6: Descriptive Statistics of Angular Measurements of Experimental Sample (degrees) – Total Sample (n=96) Right Canine Frontal Angle Left Canine Frontal Angle Right Canine Lateral Angle Left Canine Lateral Angle Mean (SD) 8.8 +/- 4.8 Media n 8.6 Mode Range (Min-Max) -4.5 25.7 (-4.5 – 21.1) -8.5 +/-4.8 -8.9 .00 25.3 (-18.9 – 6.3) 104.5 +/- 5.3 104.5 106.1 27.4 (90.8 – 118.2) 105.2 +/-5.6 105.0 104.6 26.5 (90.6 – 117.1) 53 A Student t-Test was completed to determine statistically significant differences in all parameters between genders. Table 3.7: Comparison of Gender Difference in Experimental Sample Right Canine Cusp X Right Canine Cusp Y Right Canine Cusp Z Right Canine Apex X Right Canine Apex Y Right Canine Apex Z Left Canine Cusp X Left Canine Cusp Y Left Canine Cusp Z Left Canine Apex X Left Canine Apex Y Left Canine Apex Z Males (n=48) Mean SD -17.7 1.4 Females (n=48) Intergroup Differences Mean -17.4 SD 1.4 .225 -49.0 3.0 -46.4 3.0 .000* 10.5 3.5 10.8 3.2 .640 -14.5 1.7 -13.6 1.7 .008* -24.5 2.6 -24.5 2.9 .987 4.4 2.5 5.1 2.4 .159 17.5 1.4 16.8 1.3 .016* -48.9 2.8 -46.5 3.0 .000* 10.4 3.3 10.9 3.1 .408 14.6 1.7 13.1 1.5 .000* -24.3 2.5 -24.5 3.1 .784 4.1 2.6 4.7 2.2 .164 *Denotes statistically significant (p<0.05). The results of the Student T-Test show statistically significant gender differences between several factors including right and left cups tip Y coordinates, right and left canine apex X coordinates, and the left canine cusp X coordinate. 54 A paired sample t-test was used to determine any differences between the right and left side canine position in the experimental sample. Table 3.8: Comparison of Right and Left Maxillary Canine Position of Experimental Sample (n=96) Correlation t-test Paired Differences r P Mean S.D. P Canine .121 .240 .36 1.86 .058 Cusp X* Canine .954 .000 -.04 .97 .722 Cusp Y Canine .955 .000 -.01 .99 .886 Cusp Z Canine .232 .023 .21 2.20 .354 Apex X* Canine .845 .000 -.04 1.54 .812 Apex Y Canine .877 .000 .30 1.21 .017† Apex Z *Absolute values used to relate distance from midline. †Denotes statistically significant (p<0.05). A strong correlation exists between the Y and Z coordinates for the cusp and apex. A weak correlation exists between the X coordinates of the cusp and apex. only coordinate to have a statistically significant difference between the right and left side was the Z coordinate of the canine apex. 55 The Scatter Plot Data Scatter plots of the Bolton standard (n=1) and the experimental sample (n=96) were generated to create a visual representation of the canine coordinate data. 0 -25 -20 -15 -10 -5 0 -10 -20 X-Y Right Canine Cusp Experimental Sample X-Y Right Canine Cusp Bolton Standard -30 -40 -50 -60 Figure 3.8: Scatter Plot of XY right canine cusp tip. 56 0 -25 -20 -15 -10 -5 0 -10 X-Y Right Canine Apex Experimental Sample -20 X-Y Right Canine Apex Bolton Standard -30 -40 -50 -60 Figure 3.9: Scatter plot of XY axis of right canine root apex. 57 0 0 5 10 15 20 25 -10 Z-Y Right Canine Cusp Experimental Sample Z-Y Right Canine Cusp Bolton Standard -20 -30 -40 -50 -60 Figure 3.10: Scatter Plot of ZY axis of right canine cusp tip. 58 0 -5 0 5 10 15 20 25 Z-Y Right Canine Apex Experimental Sample -10 Z-Y Right Canine Apex Bolton Standard -20 -30 -40 -50 -60 Figure 3.11: Scatter Plot of ZY axis of right canine root apex. 59 25 X-Z Right Canine Cusp Experimental Sample 20 X-Z Right Canine Cusp Bolton Standard 15 10 5 0 0 -5 -10 -15 -20 -25 Figure 3.12: Scatter plot of XZ axis of right canine cusp tip. The points are on a horizontal plane from a superior view. 60 25 X-Z Right Canine Apex Experimental Scatter 20 X-Z Right Canine Apex Bolton Standard 15 10 5 0 0 -5 -10 -15 -20 -25 -5 Figure 3.13: Scatter plot of XZ root apex. The points are on a horizontal plane from a superior view. Maxillary Canine Position Patterns The XY position of the canine cusp tip is ovoid in shape with the superior-inferior position of the canine having nearly twice the range of the medial-lateral position. The XY position of the root apex however is more circular in shape, with superior-inferior range only 3 mm greater than the medial-lateral position range. In both instances a high density of experimental subjects are very 61 near the normal position of the cusp tip and root apex. The pattern of the YZ position of the canine cusp tip resembles a parallelogram and has a similar range of superior-inferior and anterior-posterior position. The pattern of the YZ position of the root apex resembles a square. The density of the experimental subjects in proximity to the norm is much less when examining the canine cusp tip compared to the root apex. The pattern of the XZ canine cusp tip is rectangular in shape with the anterior-posterior position having twice the range of the medial-lateral. The XZ pattern of the root apex is also rectangular in shape with the anterior-posterior position slightly larger than the medial-lateral position. In both instances the highest density of experimental subjects is in the central portion of the scatter, however in both the control position of the maxillary canine cusp tip and root apex is more medial and posterior. 62 Esthetic Results The raters who completed the Q-sort generated 9 columns based on his/her esthetic preference. To calculate the results of the Q-sort, each subject was assigned a point value from 1 (least esthetic) to 9 (most esthetic) based on which column the rater placed the subject. The total point value of each subject was calculated by adding the results of all 9 Q-sorts. The subjects were ranked and a histogram of the total esthetic results was created. Histogram of Esthetic Q-Sort Result 25 Frequency 20 15 10 Frequency 5 0 0 10 20 30 40 50 60 70 80 More Bin Figure 3.14: Histogram of the total esthetic scores of all the subjects. The Kurtosis of the histogram is -0.42 and the skewness is 0.10. 63 Esthetic Results in Relation to Canine Position The linear distance from the norm (Bolton standard) of each subject was determined by right angle trigonometry. The ranks of the individuals were applied to the same frequency distribution as the Q-sort to create equal distributions. The histograms of the linear distances of each subject from the norm were generated and the results are reported in Appendix A. The linear distances from the norm were ranked and frequencies were created that were identical in distribution to the esthetic Q-sort. The canine position of the four best and four worst esthetic outcomes were evaluated by determining the frequency score. The frequency score was calculated by recording the score of the column where each subject was located in relation to the total distribution. 64 Esthetic Scores (X) vs. Total Canine Position (Y) 50 45 40 35 30 25 20 15 10 5 0 y = -0.0032x + 29.229 R² = 1E-04 Esthetic Scores vs. Canine Position Linear (Esthetic Scores vs. Canine Position) 0 20 40 60 80 100 Figure 3.15: Graph and correlation of the four subjects with the highest combined esthetic scores, four subjects with average combined esthetic scores, and the four subjects with the lowest combined esthetic scores in relation to total canine position coordinate scores Esthetic Scores (X) vs. XY Canine Position (Y) 18 16 y = 0.0011x + 9.8644 R² = 7E-05 14 12 Esthetic Scores (X) vs. Canine Position (Y) 10 8 Linear (Esthetic Scores (X) vs. Canine Position (Y)) 6 4 2 0 0 20 40 60 80 100 Figure 3.16: Graph and correlation of the four subjects with the highest combined esthetic scores, four subjects with average combined esthetic scores, and the four subjects with the lowest combined esthetic scores in relation to XY axis canine position coordinate scores. 65 Esthetic Scores (X) vs. ZY Canine Position (Y) 20 18 16 14 12 10 8 6 4 2 0 y = 0.0039x + 9.8198 R² = 0.0008 Esthetic Scores (X) vs. ZY Canine Position (Y) Linear (Esthetic Scores (X) vs. ZY Canine Position (Y)) 0 20 40 60 80 100 Figure 3.17: Graph and correlation of the four subjects with the highest combined esthetic scores, four subjects with average combined esthetic scores, and the four subjects with the lowest combined esthetic scores in relation to ZY axis canine position coordinate scores. Esthetic Scores (X) vs. XZ Canine Position (Y) 14 y = -0.0083x + 9.5448 R² = 0.0057 12 10 Esthetic Scores (X) vs. XZ Canine Position (Y) 8 6 Linear (Esthetic Scores (X) vs. XZ Canine Position (Y)) 4 2 0 0 20 40 60 80 100 Figure 3.18: Graph and correlation of the four subjects with the highest combined esthetic scores, four subjects with average combined esthetic scores, and the four subjects with the lowest combined esthetic scores in relation to XZ axis canine position coordinate scores. 66 Table 3.9: Correlations of Esthetic Scores and Maxillary Canine Position Scores Total Canine XY Canine ZY Canine XZ Canine Position Position Position Position 2 R 0.0001 0.00007 0.0008 0.0057 Graphs of the canine positions of the four best and four worst esthetic outcomes were compared to the Bolton Standard Template in the XY and YZ axis and are in Appendix B. Esthetic scores of the four best, four worst, and average total canine positions were also evaluated by determining the frequency score. A correlation of the subjects with the best total canine position score was run against their respective esthetic scores. Total Canine Position Scores (X) vs. Esthetic Scores (Y) 70 60 y = 0.2384x + 33.321 R² = 0.0347 50 40 30 Canine Position Scores (X) vs. Esthetic Scores 20 10 0 0 10 20 30 40 50 Linear (Canine Position Scores (X) vs. Esthetic Scores) Figure 3.19: Graph and correlation of the four subjects with the highest combined esthetic scores, four subjects with average combined esthetic scores, and the four subjects with the lowest combined esthetic scores in relation to ZY axis canine position coordinate scores. 67 The pictures of the subjects with the best and worst esthetic outcomes are in Appendix C and the pictures of the subjects with the best and worst total canine position outcomes are in Appendix D. 68 Discussion The purpose of this study was to evaluate the maxillary canine position in relation to maxillary skeletal position in a normal and a treated population. The influence of maxillary canine position on dental esthetics was also evaluated. Three Dimensional Assessment of Maxillary Canine Position To establish control data, a Cartesian coordinate system was implemented to determine the maxillary canine position of the Bolton Standard Template. A similar Cartesian coordinate system was utilized on a sample of CBCT volumes. A reliability study found the orientation and digitization method utilized in this study to be reliable. Descriptive statistics of the average three dimensional positional data of the maxillary canines was determined. The only statistically significant difference between the right and left canine position was between the z coordinates of the canine apex. Gender differences in canine position were assessed as well. Statistically significant differences were found in the right and left canine cusp y coordinates, the right and left canine apex x 69 coordinates, and the left canine cusp x coordinate. The reason for these differences is unclear. The large sample size of the experimental sample provided an insight into the range of maxillary canine positions in three dimensions. The scatter plots offered visualizations of the anatomic distribution of the maxillary canine cusp tip and root apex. The range of the medial-lateral and anterior-posterior position of the canine cusp was smaller than the root apex. Conversely, the range of the superior-inferior position of the canine apex is smaller than the cusp tip. The reason behind the differences in ranges of the positions is unclear. All the CBCT volumes studied were taken after orthodontic treatment. Tooth movement is initiated at the level of the crown, where the bracket is attached. Orthodontic treatment typically is not completed without coupling of the maxillary canine to the mandibular dentition. Moving the maxillary canine into a position dictated in part by the mandibular dentition potentially reduces the influence of the maxillary skeletal anatomy on canine position. Changes in root morphology occur during orthodontic treatment due to root resorption and maturation of the root. The morphology of the canine crown is dictated prior to starting orthodontic therapy and barring significant 70 restorations or recontouring should not change during the course of treatment. A matched sample of pre-treatment CBCT volumes could provide more insight into treatment effects on canine position. Without this information or a three-dimensional norm definitive conclusions cannot be determined. The lateral angular measures of both the Bolton Standard and the experimental sample were 99 and 105 degrees respectively. This data conflicted with Simon’s Orbital Plane theory as the canines in the experimental sample were more protrusive and less upright. Oppenheim‘s study on a large sample (n=159) of dry skulls found the lateral canine to average 104.5 degrees, which is in near perfect agreement with our study. The frontal canine angle of the experimental sample was 8.8 degrees for the right canine and 8.5 degrees the left which was slightly more upright than the norm values. The author was unable to find any other study examining the frontal canine angle for comparison. Maxillary Canine Position and Esthetic Perception The influence of maxillary canine position and esthetic perception was assessed. The Bolton Standard was considered to be the ideal for canine position, dental 71 esthetics, and facial esthetics. The linear distance from the ideal was determined of all experimental subjects in all three axes. Normal distributions of the distances from the ideal were created and a scoring system was implemented to grade the subjects on their position within the frequency. The distances from the norms and the position of the subjects within the frequencies were correlated to the four best, the four worst, and four average esthetic outcomes. No correlation was found between esthetic outcome and maxillary canine position. Esthetic outcomes were compared to the three principal axes (XY, YZ, and XZ) and again no correlation was found. No correlation was found when evaluating the best, worst, and average canine positions with esthetic outcomes. Esthetic perception is highly variable and multifactorial. Personal preferences also significantly affect esthetic perception. The Q-sort the nine judges completed did yield a relatively normal distribution of esthetic outcomes but canine position appears to have little influence on overall esthetic perception. Anatomic factors such as lip thickness, dental morphology, and gingival display affect esthetic perception. Previous studies examining maxillary anterior crown morphology supports the notion that individuals may not be examining maxillary 72 canine teeth as critically as the other anterior teeth.20 Perhaps a more effective way to determine the maxillary canine’s position on esthetics would be to alter the position digitally within one picture of a subject’s smile. This would reduce the confounding factors that influence esthetic perception among different individuals. The majority of orthodontic diagnosis is based on the lateral cephalogram, a two-dimensional representation of a three-dimensional object. Technological advances such as CBCT scans, three-dimensional soft-tissue rendering, and CAD/CAM study models may start to shift attention away from the midline and towards the dentofacial complex as a whole. Improved treatment methods such as skeletal anchorage and orthognathic surgery afford the clinician unprecedented control of the dentition and supporting tissues. With continued research in this field an orthodontist will be able to maximize treatment outcomes and best serve his/her patients. 73 Conclusion The post-treatment position of the maxillary canine had a statistically significant difference between the left and right anterior-posterior position of the apex. Statistically significant gender differences were found for the superior-inferior position of the right and left canine cusp tip, the mesial-lateral right and left canine apex, and the medial-lateral left canine cusp. The lateral angulation of maxillary canine was more obtuse than originally hypothesized by Simon. The difference in frontal canine angulation was not statistically significant. No correlation was found between the subjects with the best or worst esthetic outcomes and their respective canine position. The subjects with the best canine position also had no correlation to esthetic outcomes. 74 Literature Cited 1. Burstone CJ, Nanda R. 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Am J Orthod Dentofacial Orthop. 2005;(128):458–465. 77 Appendix A Frequency Histogram 16 14 12 10 8 6 4 2 0 Frequency Bin Figure 3.20: Histogram of linear distances from the XY coordinate Bolton Standard of the right canine cusp. The kurtosis is -0.57 and the skewness is 0.35. Histogram 30 Frequency 25 20 15 10 Frequency 5 0 Bin Figure 3.21: Histogram of the linear distances from the XY coordinate of the Bolton Standard of the right canine apex. The kurtosis is 0.74 and the skewness is 0.83. 78 Frequency Histogram 16 14 12 10 8 6 4 2 0 Frequency Bin Figure 3.22: Histogram of the linear distances from the ZY coordinate of the Bolton Standard of the right canine cusp. The kurtosis is -0.66 and the skewness is 0.15. Histogram 30 Frequency 25 20 15 10 Frequency 5 0 Bin Figure 3.23: Histogram of the linear distances from the ZY coordinate of the Bolton Standard of the right canine apex. The kurtosis is 1.33 and the skewness is 0.85. 79 16 14 12 10 8 6 4 2 0 More 12 11 10 9 8 7 6 5 4 3 2 1 Frequency 0 Frequency Histogram Bin Figure 3.24: Histogram of the linear distances from the XZ coordinate of the Bolton Standard of the right canine cusp. The kurtosis is -0.25 and the skewness is 0.50. Histogram 30 Frequency 25 20 15 Frequency 10 5 0 0 1 2 3 4 5 6 7 8 9 More Bin Figure 3.25: Histogram of the linear distances from the XZ coordinate of the Bolton Standard of the right canine apex. The kurtosis is 0.36 and the skewness is 0.71. 80 Appendix B Figure 3.26: Graph of four best and worst esthetic outcome XY canine position compared to the Bolton Standard. Figure 3.27: Graph of four best and worst esthetic outcome YZ canine position compared to the Bolton Standard. 81 Appendix C Figure 3.28: scores Four subjects with highest combined esthetic 82 Figure 3.29: scores Four subjects with lowest combined esthetic 83 Appendix D Figure 3.30: Smile photographs of four subjects with highest combined canine position scores. 84 Figure 3.31: Smile photographs of four subjects with lowest combined canine position scores 85 Vita Auctoris John Katsis III was born in Hinsdale, Illinois on May 10th, 1983 to Dr. John Katsis Jr. and Joan Katsis. He has two older sisters who are teachers and one younger brother who is in medical school. He graduated from Hinsdale Central High School in 2001. He graduated with High Honors from the University of Illinois at Urbana-Champaign in May 2005 with a Bachelors of Science in Kinesiology. John began his dental education at the University of Illinois at Chicago in August of 2006. As both the son of an orthodontist and the benefactor of orthodontic treatment, he was fully aware of the personal and professional satisfaction that the field of orthodontics offered. In May of 2010 he graduated from dental school and delivered one of two student commencement speeches. He was accepted to the orthodontics residency program at Saint Louis University in December of 2009 and began his residency in June of 2010. He plans to complete his Masters of Science in Dentistry in December 2012. After graduation, John will join his father’s orthodontic practices in Bartlett and Bloomingdale, Illinois. 86