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11-281-C100 Monoclonal Antibody to Intra-Acrosomal Protein (Canine) Purified Antibody (0.1 mg) Clone: Ds-2 Isotype: Mouse IgG Specificity: The antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm. Regulatory Status: RUO Immunogen: Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization. Species Reactivity: Canine (Dog) Application: Immunocytochemistry Application note: Membrane permeabilization (acetone) is essential. Western Blotting Purity: > 95% (by SDS-PAGE) Purification: Purified from ascites by protein-A affinity chromatography. Concentration: 1 mg/ml Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Storage / Stability: Store at 2-8oC. Do not freeze. Do not use after expiration date stamped on vial label. Expiration: See vial label Lot Number: See vial label References: *Geussova G, Peknicova J, Capkova J, Kalab P, Moos J, Philimonenko VV, Hozak P: Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction. Andrologia. 1997 Sep-Oct;29(5):261-8. Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz. For laboratory research only, not for drug, diagnostic or other use. EXBIO Praha | Nad Safinou II 341 | 252 42 Vestec u Prahy | Czech Republic Tel: +420 261 090 666 | Fax: +420 261 090 660 | [email protected] | www.exbio.cz