Download BIOGRAPHICAL SKETCH

Program Director/Principal Investigator (Last, First, Middle):
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Christiane E. Wobus
Assistant professor
eRA COMMONS USER NAME (credential, e.g., agency login)
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
University of Goettingen, Germany
Michigan State University, East Lansing, MI
University of Heidelberg, Germany
University of Goettingen, Germany
Washington University School of Medicine, St.
Louis, MO
DEGREE
(if applicable)
Vordiplom
MS
Ph.D.
Vordiplom
Postdoctoral
Fellow
YEAR(s)
1992-94
1994-97
1997-2000
1992-94
2001-2006
FIELD OF STUDY
Biology
Plant Pathology
Virology, viral gene
Biology
Virology, viral
pathogenesis
A. Positions and Honors
Postionand and Employment
3/2007 - Assistant professor Microbiology and Immunology, University of Michigan Medical School, Ann
Arbor, MI
Honors
1997-2000
German Cancer Research Institute PhD fellowship
2002- 2005 Institutional NIH training grant appointment
7/2003
Travel grant – American Society for Virology
7/2004
Travel grant – American Society for Virology
11/2004 Travel grant - Second International Calicivirus Conference
6/2005
Travel grant – American Society for Virology
2005-2006 Postdoctoral fellowship, Midwest Regional Center of Excellence (MRCE) for Biodefense and
Emerging Infectious Disease Research
2006
Biological Science Scholar, University of Michigan
11/2007 Travel grant – Third International Calicivirus Conference
Professional Societies
2001 –
Member, American Society for Virology (Full member since 2007)
2006 –
Member, AAAS
2009 Member, AMS
B. original peer-reviewed publications
1. Hyde, J.L., Sosnovtsev, S.V., Green, K.Y., Wobus, C., Virgin, H.W., Mackenzie, J.M. (2009) Mouse
norovirus replication is associated with virus-induced vesicle clusters originating from membranes derived from
the secretory pathway. Journal of Virology 83, 9709-19. PMID: 19587041
2. Perry, J. W., Taube, S., Wobus, C. E. (2009) Murine Norovirus-1 entry into permissive macrophages and
dendritic cells is pH-independent. Virus Research 43, 125-9. PMID: 19463729
3. Taube, S., Perry, J. W., Yetming, K., Patel, S. P., Auble, H., Shu, L., Nawar, H. F., Lee, C. H., Connell, T.
D., Shayman, J. A., Wobus, C. E. (2009) Ganglioside-linked terminal sialic acid moieties on murine
macrophages function as attachment receptors for Murine Noroviruses (MNV). Journal of Virology 83, 4092101. PMID: 19244326
4. Chachu, K. A.,Strong, D. W., LoBue, A. D., Wobus, C. E., Baric, R. S., Virgin, H. W. 4th. (2008) Antibody is
critical for the clearance of MNV infection. Journal of Virology 82, 6610-7.
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Program Director/Principal Investigator (Last, First, Middle):
PI Name
5. Katpally, U., Wobus, C. E., Dryden, K., Virgin, H. W. 4th, Smith, T. J. (2008) The Structure of Antibody
Neutralized Murine Norovirus and Unexpected Differences to Virus Like Particles. Journal of Virology 82, 2079882.
6. Baert, L., Wobus, C. E., Van Coillie, E., Thackray, L. B., Debevere, J., Uyttendaele, M. (2008) Detection of
Murine Norovirus 1 using plaque assay, transfection assay and real-time RT-PCR before and after heat
exposure. Applied Environmental Microbiology 74, 543-6.
7. Thackray, L. B., Wobus, C. E., Chachu, K. A., Liu, B., Alegre, E. R., Henderson, K. S., Kelley, S. T., Virgin,
H. W. 4th (2007) Murine noroviruses comprising a single genogroup exhibit biological diversity despite limited
sequence divergence. Journal of Virology 81, 10460-10473.
8. Ward, V. K., McCormick, C. J., Clarke, I. N., Salim, O., Wobus, C. E., Thackray, L. B., Virgin, H. W. 4th,
Lambden, P. R. (2007) Recovery of infectious murine norovirus using pol II-driven expression of full-length
cDNA. PNAS 104, 11050-11055.
9. Mumphrey, S. M., Changotra, H., Moore, T. N., Heimann-Nichols, E. R., Wobus, C. E., Reilly, M. J.,
Moghadamfalahi, M., Shukla, D., Karst, S. M. (2007) Murine norovirus 1 infection is associated with
histopathological changes in immunocompetent hosts, but clinical disease is prevented by STAT1-dependent
interferon responses.
Journal of Virology 81, 3251-63.
10. Ward, J.M., Wobus, C. E., Thackray. L. B., Erexson, C. R., Faucette, L. J., Belliot, G., Barron, E. L.,
Sosnovtsev, S. V., Green, K. Y. (2006) Pathology of immunodeficient mice with naturally occurring murine
norovirus infection.
Toxicological Pathology 34, 708-15.
11. Sosnovtsev, S. V., Belliot, G., Chang, K. O., Prikhodko, V. G., Thackray, L. B., Wobus, C. E., Karst, S. M.,
Virgin, H. W., Green, K. Y. (2006) Cleavage map and proteolytic processing of the murine norovirus
nonstructural polyprotein in infected cells. Journal of Virology 80, 7816-31.
12. Daughenbaugh, K. F., Wobus, C. E., Hardy, M. E. (2006) Murine norovirus VPg binds translation initiation
factors in infected cells. Virol Journal 23;3-33.
13. Wobus, C. E., Thackray, L. B., Virgin, H. W. 4th (2006) Murine norovirus: a model system to study
norovirus biology and pathogenesis. Journal of Virology 80, 5104-12.
14. Hsu, C.C., Wobus, C. E., Steffen, E. K., Riley, L. K., Livingston, R. S. (2005) Development of a
microsphere-based serologic multiplexed fluorescent immunoassay and reverse transcriptase-PCR assay to
detect Murine Norovirus 1 (MNV-1) infection in mice. Clinical and Diagnostic Laboratory Immunology 12, 114551.
15. Wobus, C. E., Karst S. M., Thackray, L. B., Chang, K.-O., Sosnovstev S., Belliot, G., Krug, A., Mackenzie,
J. M., Green., K. Y., Virgin, H. W. (2004) Replication of a norovirus in culture reveals a tropism for dendritic
cells and macrophages. PLOS Biology 2. e432.
16. Karst S. M.*, Wobus, C. E.*, Lay, M., Davidson, J., Virgin, H. W. (2003) STAT-1 dependent innate
immunity to a Norwalk-like virus. Science 299, 1575-1578.
17. Kern, A., Schmidt, K., Leder, C., Müller, O.J., Wobus, C. E., Bettinger, K., Von der Lieth, C. W., King, J. A.,
and J. A. Kleinschmidt. (2003) Identification of a heparin-binding motif on adeno-associated virus type 2
capsids. Journal of Virology 77, 11072-81.
18. Girod A.*, Wobus C.E.*, Z. Zadori, M. Ried, K. Leike, P. Tijssen, J. A. Kleinschmidt, M. Hallek. (2002) The
VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus
infectivity. Journal of General Virology 83, 973-978.
19. Wobus, C. E., Hügle-Dörr, B., Girod, A., Petersen, G., Hallek, M. and J. A. Kleinschmidt. (2000)
Monoclonal antibodies against the AAV-2 capsid: epitope mapping and identification of capsid domains
involved in AAV-2 - cell interaction and neutralization of AAV-2 infections. Journal of Virology 74, 9281-9293.
20. Girod, A., Ried, M., Wobus, C., Lahm, H., Leike, K., Kleinschmidt, J., Deleage, G., and M. Hallek. (1999)
Genetic capsid modifications allow efficient re-targeting of adeno-associated virus type 2. Nature Medicine 5,
1052-1056.
21. Wobus, C. E., Skaf, J. S., Schultz, M. H., and G. A. de Zoeten. (1998) Sequencing, genomic localization
and initial characterization of the VPg of pea enation mosaic enamovirus. Journal of General Virology 79,
2023-2025.
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Program Director/Principal Investigator (Last, First, Middle):
PI Name
22. Skaf, J. S., Rucker, D. G., Demler, S. A., Wobus, C. E., and G. A. de Zoeten. (1997) The coat protein is
dispensable for the establishment of systemic infections by pea enation mosaic enamovirus. Plant Molecular
Interactions 10, 929-932.
C. Research Support
Ongoing Research Support
Start-up funds Wobus (PI)
3/2007
Department of Microbiology and Immunology, University of Michigan Medical - School
Rackham faculty research grant Wobus (PI)
3/2008 – 3/2010
University of Michigan
“Mechanism of norovirus interaction with intestinal monolayers”
Goal: The goal of this project is to determine whether murine norovirus crosses the intestinal epithelial
monolayer using M cells and/or DCs and identify the mechanism of non-immune IgA mediated MNV
transcytosis in an in vitro murine intestinal epithelial model.
Role: PI
Career Development grant Wobus (PI)
3/2008-2/2010
Great Lakes Regional Center for Excellence in Biodefense and Emerging Infectious Diseases Research
NIAID, NIH
“Identification of small molecule inhibitors and novel drug targets for noroviruses”
Goal: The goal of this project is to identify small molecule inhibitors of norovirus entry and identify the cellular
and viral determinants of norovirus transcytosis in an in vitro murine intestinal epithelial model.
Role: PI
Elizabeth Crosby faculty grant Wobus (PI)
11/2008-10/2009
University of Michigan
“The role of sialic acid during murine norovirus infection in vitro and in vivo”
Goal: This grant provides salary support to work on a project that aims to elucidate the role of sialic acid
containing proteins and lipids during MNV infection of murine macrophages in culture and in a mouse model.
Role: PI
University of Michigan Wobus (PI)
2/2009-1/2010
2008 Genetics and Genomics Pilot Feasibility Grant Program – 2nd Round,
“16S genetic analysis of the gastrointestinal microbiome following enteric norovirus infection”
Goal: This project funds the investigation of changes in the intestinal microbiota following norovirus infection
using 454 high-throughput sequencing.
Role: PI
R01 Wobus (PI)
07/24/2009 – 06/30/2013
NIH, NIAID
“Mechanisms of norovirus entry”
Goal: The aim of this project is to dissect viral and host determinants of murine norovirus entry in culture and
in mice.
Role: PI
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